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• Regulation of hair cell and stomatal size by a hair-cell specific peroxidase in the grass Brachypodium distachyon

  Tiago D. G. Nunes, et al., bioRxiv - Plant Biology 2023

  Quote: ... 600μl CuSO4 (1mg/ ml, Sigma/Merck #C3036), 500μl 2,4-D (5mg/ml in 1M KOH ...
• Regulation of hair cell and stomatal size by a hair-cell specific peroxidase in the grass Brachypodium distachyon

  Tiago D. G. Nunes, et al., bioRxiv - Plant Biology 2023

  Quote: ... and sterile Kinetin solution (0.2μg/ml final conc., Sigma/Merck #K3253). Calli were incubated at 28°C and a 16h light:8h dark cycle (70-80 μmol PAR m-2 s-1) ...
• Regulation of hair cell and stomatal size by a hair-cell specific peroxidase in the grass Brachypodium distachyon

  Tiago D. G. Nunes, et al., bioRxiv - Plant Biology 2023

  Quote: ... 500μl 2,4-D (5mg/ml in 1M KOH, Sigma/Merck #D7299), pH 5.8 ...
• Chronic cholesterol administration to the brain supports complete and long-lasting cognitive and motor amelioration in Huntington’s disease

  Giulia Birolini, et al., bioRxiv - Neuroscience 2023

  Quote: ... followed by incubation with anti-EM48 primary antibody (1:100; Merck Millipore, cat n° MAB5374) for 3h at RT ...
• Regulation of hair cell and stomatal size by a hair-cell specific peroxidase in the grass Brachypodium distachyon

  Tiago D. G. Nunes, et al., bioRxiv - Plant Biology 2023

  Quote: ... plus 2.1g of Phytagel (Sigma/Merck #P8169)) ...
• Valproic acid exposure affects social visual lateralization and asymmetric gene expression in zebrafish larvae

  Andrea Messina, et al., bioRxiv - Neuroscience 2023

  Quote: ... At 5 hpf embryos were placed into 10 cm Petri dishes containing E3 medium (control) and E3 medium with 1 μM VPA (Sigma-Aldrich, P4543; Merck Life Science Srl, Milan, Italy) for 24 or 48 h ...
• Promyelocytic Leukemia Protein regulates Angiogenesis and Epithelial-Mesenchymal Transition to limit metastasis in MDA-MB-231 breast cancer cells

  Amalia P Vogiatzoglou, et al., bioRxiv - Cancer Biology 2023

  Quote: ... Cell culture 8μm inserts (Merck KGaA, Germany) in FBS-free medium overlaying the lower compartment filled with FBs-containing media ...
• Promyelocytic Leukemia Protein regulates Angiogenesis and Epithelial-Mesenchymal Transition to limit metastasis in MDA-MB-231 breast cancer cells

  Amalia P Vogiatzoglou, et al., bioRxiv - Cancer Biology 2023

  Quote: ... stained with crystal violet (Merck) and counted under a microscope ...
• Promyelocytic Leukemia Protein regulates Angiogenesis and Epithelial-Mesenchymal Transition to limit metastasis in MDA-MB-231 breast cancer cells

  Amalia P Vogiatzoglou, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The secondary antibody was washed again three times with PBS and the cell nuclei were then counter stained with DAPI (Merck). Epifluorescence microscopy was done in an inverted Olympus IX70 and Confocal microscopy was done in a Zeiss Axioscope 2 Plus microscope equipped with a Bio-Rad Radiance 2100 laser scanning system and Lasersharp 2000 imaging software and a Leica SP8 inverted focus microscope and analyzed with the Leica Application Suite (Las ...
• Promyelocytic Leukemia Protein regulates Angiogenesis and Epithelial-Mesenchymal Transition to limit metastasis in MDA-MB-231 breast cancer cells

  Amalia P Vogiatzoglou, et al., bioRxiv - Cancer Biology 2023

  Quote: Tissues isolated from experimental animals were fixed with 10% formalin (Merck) and kept overnight at 4° C ...
• A Computational Approach Reveals the Ability of Amyloids to Sequester RNA: the Alpha Synuclein Case

  Jakob Rupert, et al., bioRxiv - Biochemistry 2023

  Quote: ... total DNA from herring sperm (Sigma Merck) was resuspended in sterile ...
• Trellis Single-Cell Screening Reveals Stromal Regulation of Patient-Derived Organoid Drug Responses

  María Ramos Zapatero, et al., bioRxiv - Cancer Biology 2023

  Quote: ... 5-FU (Merck F6627), Oxaliplatin (Merck O9512) ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: ... NP-Osu (Biosearch, cat no: N-1010-100) was dissolved in dimethylformamide (DMF) (Merck) to a concentration of 10mg/ml while vortexing ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: Isolated cells were transferred onto 18mm coverslips coated with 0.001% poly-L-lysine (Merck, cat: P4707-50ML, 10× stock) and incubated for 10 mins at 37°C to ensure cell attachment ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: ... Chloramphenicol (Merck, cat no: C0378-5G) was used at 10μg/ml and 25μg/ml concentrations (prepared in 100% ethanol fresh for each culture experiment ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: ... and Ru265 (30μM, Merck). After five hours ...
• Dynamic mitochondrial transcription and translation in B cells control germinal centre entry and lymphomagenesis

  Yavuz F Yazicioglu, et al., bioRxiv - Immunology 2023

  Quote: ... with or without mitoTempo (100μM, Merck) and Ru265 (30μM ...
• On the evolution of the plant phytochrome chromophore biosynthesis

  Federica Frascogna, et al., bioRxiv - Plant Biology 2023

  Quote: ... a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa, Merck).
• On the evolution of the plant phytochrome chromophore biosynthesis

  Federica Frascogna, et al., bioRxiv - Plant Biology 2023

  Quote: ... after concentrating the protein (Amicon® Ultra MWCO 30 kDa, Merck, Darmstadt, Germany), a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... the brain was incubated in 0.5% Lucifer Yellow CH dilithium salt (Merck KGaA, Darmstadt, Germany) diluted in 0.1 M PBS overnight on a shaker at 4 °C and washed again with 0.1 M PBS (5x 10 min ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: Ten odors we used for stimulation: 1-hexanol (Merck KGaA, Darmstadt, Germany), 1-heptanol (Merck KGaA ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... & trans-cinnamaldehyde (Merck KGaA, Darmstadt, Germany). This set of odors is composed of single molecular odors that are perceivable by cockroaches (Sakura et al. ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: Cells arrested at G2 using RO-3306 (Merck) at 7μM final concentration were released and allowed to continue with cell cycle for 1 hour ...
• Acetyl transferase EP300 deficiency leads to chronic replication stress mediated by defective fork protection at stalled replication forks

  Angelica Barreto-Galvez, et al., bioRxiv - Cancer Biology 2023

  Quote: ... asynchronous cells were treated with CDK1 inhibitor RO-3306 (Merck) at a final concentration 7 μM for 7:30 h ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... chicken polyclonal anti-NFH antibody (Merck, AB5539, 1:200 dilution). The following secondaries were utilized for neural cell and breast cancer tissue staining ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... neural cells were stained with rabbit polyclonal anti-tyrosine hydroxylase antibody (Merck, AB152, 1:200 dilution), guinea pig polyclonal anti-DCX antibody (Merck ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... 0.5 mM EDTA and 0.5 mg/ml L-cysteine (#30090, Merck), in DMEM ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... We discarded the supernatant and carefully resuspended the pellet in 800 μl M2B-taxol (M2B supplemented with 10 μM taxol (T7402, Merck, Darmstadt, Germany).
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... The brains were then fixed with 4% PFA (#30525894, Merck) in PBS ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... with 1 mM CaCl2 (#A862982745, Merck), 0.5 mM EDTA and 0.5 mg/ml L-cysteine (#30090 ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... consisting of 10 mM MES (#M3671, Merck), 138 mM KCl (#K42209636128 ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... in Dulbecco’s Modified Eagle Medium (DMEM #D5671, Merck, Darmstadt, Germany), with the addition of 10% FCS (fetal calf serum ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... with the addition of 10% FCS (fetal calf serum, #S0615, Merck) and 4 mM glutamine (#25030-024 ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... Unreacted aldehyde groups were quenched using 0.1% NaBH4 (#71320, Sigma Aldrich now Merck), for 7 minutes in PBS ...
• Visualizing proteins by expansion microscopy

  Ali H. Shaib, et al., bioRxiv - Biophysics 2023

  Quote: ... cells were grown overnight on poly-L-lysine-coated coverslips (#P2658, Merck).
• The negative adipogenesis regulator DLK1 is transcriptionally regulated by TIS7 (IFRD1) and translationally by its orthologue SKMc15 (IFRD2)

  Ilja Vietor, et al., bioRxiv - Cell Biology 2023

  Quote: ... 1%(w/v) Triton X-100 (Merck), 2 mM EDTA) ...
• Shear rheology of methyl cellulose based solutions for cell mechanical measurements at high shear rates

  Beyza Büyükurgancı, et al., bioRxiv - Biophysics 2023

  Quote: ... the solutions were sterile filtered using membrane filters (Merck Millipore Steritop-GP polyethersulfone (PES ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1-heptanol (Merck KGaA, Darmstadt, Germany), 1-octanol (Thermo Fisher Scientific ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1-pentanol (Merck KGaA, Darmstadt, Germany), heptanal (Thermo Fisher Scientific ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... benzaldehyde (Merck KGaA, Darmstadt, Germany) & trans-cinnamaldehyde (Merck KGaA ...
• The mushroom body output encodes behavioral decision during sensory-motor transformation

  Cansu Arican, et al., bioRxiv - Neuroscience 2023

  Quote: ... octanal (Merck KGaA, Darmstadt, Germany), 2-heptanone (Thermo Fisher Scientific ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... guinea pig polyclonal anti-doublecortin antibody (Merck, AB2253, 1:200 dilution), chicken polyclonal anti- NFH antibody (Merck ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... mouse monoclonal anti-NeuN antibody (Merck, MAB377, 1:200 dilution), mouse monoclonal anti-Nestin antibody (CST ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The primary antibodies guinea pig polyclonal anti-doublecortin antibody (Merck, AB2253, 1:200 dilution), mouse monoclonal anti-CKAE1/AE3 (Dako ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... rabbit polyclonal anti-INA antibody (Merck, AB5354, 1:200 dilution), mouse monoclonal anti-NeuN antibody (Merck ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... chicken polyclonal anti- NFH antibody (Merck, AB5539, 1:200 dilution) overnight at 4 degrees ...
• Global and single-cell proteomics view of the co-evolution between neural progenitors and breast cancer cells in a co-culture model

  Ole Vidhammer Bjørnstad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... guinea pig polyclonal anti-DCX antibody (Merck, AB2253, 1:200 dilution), rabbit polyclonal anti-INA antibody (Merck ...
• Time-gated Fluorescence Lifetime Imaging in the Near Infrared Regime; A Comprehensive Study Toward In Vivo Imaging

  Meital Harel, Uri Arbiv, Rinat Ankri, bioRxiv - Bioengineering 2023

  Quote: ... then loaded onto glass slides spaced with silicon isolator sheets (Merck industries, Israel). The 3D plots of the excitation and emission spectra ...
• Time-gated Fluorescence Lifetime Imaging in the Near Infrared Regime; A Comprehensive Study Toward In Vivo Imaging

  Meital Harel, Uri Arbiv, Rinat Ankri, bioRxiv - Bioengineering 2023

  Quote: ... The dye solutions were placed between two glass slides separated by a silicon insulator film (Merck, Israel). The emitted fluorescence was recorded with a NIR objective lens (5018-SW ...
• The MAPK/ERK channel capacity exceeds 6 bit/hour

  Paweł Nałęcz-Jawecki, et al., bioRxiv - Systems Biology 2023

  Quote: ... hydrocortisone (0.5 μg/ml, Sigma-Aldrich/Merck).