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Citations for Merck :
6051 - 6100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... the reaction buffer was exchanged to the lysis buffer five times by Amicon Ultra 10K (Merck Millipore). Then ...
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Docking Domain Engineering in a Modular Polyketide Synthase and its Impact on Structure and FunctionbioRxiv - Biochemistry 2023Quote: ... Proteins were concentrated with Amicon Ultra centrifugal filters from Merck Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Clarified lysates were normalised across samples and were added to 100 μl anti-FLAG M2 affinity gel (Merck) Micro Bio-Spin columns (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal FLAG-HRP (Sigma-Aldrich (Merck), A8592) ...
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bioRxiv - Biochemistry 2023Quote: Total RNA extraction was performed using the TRI Reagent (Merck, product code T9424) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: ... 0.2 % cholesteryl hemisuccinate (CHS) (Merck) and rotated for 1 h at 4 °C.
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bioRxiv - Biochemistry 2023Quote: Luria-Bertani (LB) agar (Merck) plates were inoculated with Saccharomyces cerevisiae ...
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bioRxiv - Biochemistry 2023Quote: ... Desalting was performed with C18 Zip Tips (Merck) as per manufacturer guidelines ...
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bioRxiv - Biochemistry 2023Quote: ... Buffer exchange to 1xHBS and protein concentration was conducted by ultrafiltration (Amicon Ultra 10 kDa MWCO, Merck).
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bioRxiv - Biochemistry 2023Quote: ... 150 mM NaCl) and concentrated by ultrafiltration (Amicon Ultra 50 kDa MWCO, Merck).
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bioRxiv - Biochemistry 2023Quote: ... sodium acetate and formic acid (FA) were obtained from Merck (Darmstadt, DE). Acetonitrile and 0.1% FA were obtained from Biosolve (Valkenswaard ...
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bioRxiv - Biochemistry 2023Quote: ... followed by an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck). The sample was transferred to a 5 mm NMR-tube (Labscape™ Essence ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were deproteinized with a 3 kDa MWCO concentrator (Merck Millipore). To measure CoA levels ...
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bioRxiv - Biochemistry 2023Quote: ... at 4 °C for 30 s and concentrated in a 100 kDa Amicon Ultra centrifugal filter (Merck Millipore), which was equilibrated with 1% GDN in IP buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting sample of holo-SmLPMO10A was concentrated to ~ 150 μM using an Amicon ®Ultra centrifugation filter (3 kDa cut-off, Merck) and the sample was split into three equal volumes of ~ 500 μL ...
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bioRxiv - Biochemistry 2023Quote: Milli-Q water was generated from Merck Milli-Q IQ 7003 system (Darmstadt ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
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bioRxiv - Biochemistry 2023Quote: ... The peak fractions containing the protein were pooled and concentrated in centrifugal concentrators (CLVG01230, Merck Millipore, Cork, Ireland) to a final concentration of 30 mg/ml for crystallization ...
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bioRxiv - Biochemistry 2023Quote: ... PYR-41 (Merck, 662105) or Bafilomycin A1 (Biozol ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were treated with stock solutions of MG-132 (Merck, 474790), PYR-41 (Merck ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Biochemistry 2023Quote: ... then transferred to a polyvinylidene difluoride (PVDF) membrane (IPVH00010; Merck Millipore, Burlington, MA, USA). Antibodies against UFM1 (ab109305 ...
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bioRxiv - Biochemistry 2023Quote: ... 200 μl of IP buffer and 10 μl of anti-FLAG M2 Affinity Agarose Gel (A2220, Merck Millipore) were added to the 200 μl of lysate ...
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bioRxiv - Biophysics 2023Quote: ... After transferring the proteins onto a PVDF membrane (Merck Millipore), the membranes were blocked in TBS-T (20 mM Tris ...
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bioRxiv - Biophysics 2023Quote: ... or FGFJI (20 ng ml−1, bFGF, R&D Systems; and 200 nM JAK inhibitor, Calbiochem, Merck Millipore, Germany). Cells were collected for mechanical characterization and RNA-Seq after 5-day exposure to the respective culture conditions (30).
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bioRxiv - Biophysics 2023Quote: ... and 50% formamide (Merck, S4117) for 30 minutes in RT ...
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bioRxiv - Biophysics 2023Quote: ... and ECL films (GE28-9068-37, Merck Millipore). Films were developed in an OptiMax X-ray film processor (KODAK ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) fetal bovine serum, 1 mM sodium pyruvate (gibco, 11360039) and 1% antibiotic/antimycotic solution (Merck, A5955). Medium was refreshed every 3 days during cultivation and every 24h in an experimental setting ...
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bioRxiv - Biochemistry 2023Quote: ... and rabbit monoclonal K48-specific Ub (Apu2) (Merck, 05-1307). The following secondary antibodies were used ...
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bioRxiv - Biochemistry 2023Quote: ... rabbit monoclonal K63-specific Ub (Apu3) (Merck, 05-1308), and rabbit monoclonal K48-specific Ub (Apu2 ...
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bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
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bioRxiv - Molecular Biology 2023Quote: ... for 3 h or with 100 ng/ml nocodazole (M1404, Merck) for 6 h ...
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bioRxiv - Molecular Biology 2023Quote: ... soluble contents of cells were pre-extracted before fixation by incubation for 1 min in 0.1% Triton (T8787-50ML, Merck) diluted in PBS 1X (10010-015 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated the slides for 5 min in fresh 5% GIEMSA solution (1.09203, Merck) diluted in 100 ml Gurr buffer (10582-013 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then induced for EmGFP-NSD3 expression with 2 μg/ml of doxycycline (D9891-1G, Merck) for 48 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Membranes were treated with the Immobilon ECL Ultra Western HRP substrate (WBKLS0500, Merck), and signals were detected using an Amersham Imager 680 (GE Healthcare ...
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bioRxiv - Molecular Biology 2023Quote: ... Following saturation for 1 h in PBS 1X containing 5% milk and 0.1% Tween 20 (655204-100ML, Merck), the membranes were incubated overnight at 4 °C with primary antibodies ...
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bioRxiv - Molecular Biology 2023Quote: ... or using Amersham Hyperfilm ECL film (GE28-9068-35, Merck). Quantification of band signal intensity was done with Fiji’s analyse gels tool.
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bioRxiv - Molecular Biology 2023Quote: ... and 0.5 mM dithiothreitol) supplemented with EDTA-free protease inhibitor tablets (5892953001, Merck) and a homemade phosphatase inhibitor cocktail (final concentrations of 5 mM sodium fluoride ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... thymidine (T1895-1G, Merck) was added to the medium 8 h post-transfection at a final concentration of 2 mM ...
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bioRxiv - Cell Biology 2023Quote: ... and the remaining supernatants then added to 4µg rabbit anti-HA antibody (Merck). After 30 minutes incubation at 4⁰C on a rotator ...
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bioRxiv - Genomics 2023Quote: ... 4 % DDT and 2 % clothianidin-impregnated papers prepared in acetone and Dow Corning 556 Silicone Fluid (BHD/Merck, Hesse, Germany). Flies overexpressing CYP6Z2 were also exposed to the primary product of hydrolysis of pyrethroids ...
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bioRxiv - Cell Biology 2023Quote: ... media was replaced with fresh media containing 50µM Biotin (Merck) and the cells were incubated for a further 24 or 6 hours (for BirA*-RAB18 and BioID2(Gly40Ser)-SEC22A experiments respectively) ...
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bioRxiv - Microbiology 2023Quote: ... respectively (Merck, Darmstadt, Germany). From the obtained absorption value ...
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bioRxiv - Molecular Biology 2023Quote: ... immunoreaction was visualised with 3,3’-diaminobenzidine (DAB) chromogen reaction (Sigma-Aldrich, Merck) and background-stained with haematoxylin (Amresco) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cultures were treated with extravidin (Sigma-Aldrich, Merck) for 1 h at a dilution of 1:500 in TPBS ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were resuspended in a modified sucrose electroporation buffer prepared by adding 272 mM sucrose (Sigma-Aldrich, Merck) to Opti-MEM™ Reduced Serum Medium (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Distal parts of the fore and hind limb buds of embryos were pooled and dissociated in 0.25% trypsin-EDTA (Sigma-Aldrich, Merck, St. Louis, MO, USA; pH 7.4) at 37 °C for 1 h ...