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Citations for Merck :
6151 - 6200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted proteins were concentrated using Amicon spin concentrators (Merck Millipore) and subjected to gel filtration (Superdex 75 and 200 16/60 pg ...
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bioRxiv - Biochemistry 2023Quote: ... and transiently transfected with the plasmid DNA containing GFP-tagged protein of interest (See Table S7 for plasmid constructs) using GeneJuice transfection reagent (70967, Merck) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and the resulting gel disc was mounted in a live imaging chamber (Aireka Cells) with low-melt agarose (A7431, Merck). The chamber was filled with ddH2O to prevent sample shrinkage and drying ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 mM EDTA (E5134, Merck), 0.5 % Triton X-100 (T8787 ...
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bioRxiv - Molecular Biology 2023Quote: ... and 800 mM guanidine HCl (G3272, Merck) in double-distilled water (ddH2O) ...
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bioRxiv - Molecular Biology 2023Quote: ... A piece of parafilm (P7793, Merck) was glued on top of a coverslip with a drop of cyanoacrylate glue (Loctite Super Glue Power Flex Gel Control ...
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bioRxiv - Neuroscience 2023Quote: ... 2 ml EDTA tubes were prepared with 100μl protease inhibitor (Pefabloc® SC Plus, Merck KGaA, Germany) before blood collection ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... then hydrated in graded alcohol with increasing water and placed in Cresyl Violet acetate (MERCK). Sections were then dehydrated in increasing alcohol and mounted in Eukitt® Quick-hardening mounting medium (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Active (acylated) ghrelin concentrations were determined using the EZGRA-88K kit (Merck, Germany) in duplicates (total analytical CV at 488 pg/ml 12%).
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bioRxiv - Cancer Biology 2023Quote: ... and 2) Claret (Merck). The PKH-26 blasts were combined back with the claret LSCs and vice versa ...
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bioRxiv - Cancer Biology 2023Quote: ... and the membranes stained with 1) PKH-26 (Merck) and 2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody cocktail was prepared in excess and filtered through a 0.1 µm centrifugal filter column (Merck Millipore) to remove antibody aggregates.
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bioRxiv - Cell Biology 2023Quote: Cell pellets were lysed using RIPA buffer (Merck, Darmstadt, Germany) supplemented with 0.1 % (v/v ...
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bioRxiv - Biochemistry 2023Quote: Chemicals were purchased from Merck, Sigma Chemical (St ...
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bioRxiv - Biochemistry 2023Quote: ... Silica gel 60 F254 (Merck, Darmstadt, Germany) was used for TLC analysis ...
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bioRxiv - Cancer Biology 2023Quote: ... + 10% FBS (Fisher Scientific #10500064)] were plated on an ELISPOT plate (Merck Millipore #MAIP4510) coated overnight with 5 μg/mL anti-murine IFNγ capture antibody (BD #51-2525KZ) ...
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bioRxiv - Cancer Biology 2023Quote: ... formazan was solubilized by adding 2-propanol 0.1 M HCl (Sigma-Aldrich, Merck Life Science, Milan, Italy). The absorbance was measured at wavelength 570 nm (660 nm background wavelength ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967, Merck Millipore) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... dichloromethane and chloroform were purchased from Merck, (Damstadt ...
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bioRxiv - Biochemistry 2023Quote: ... hexane and ethyl acetate were purchased from Merck (Darmstadt, Germany). Tween 20 ...
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bioRxiv - Cancer Biology 2023Quote: Cell viability was also assessed with MTT assay (Millipore, Merck Life Science, Germany). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Then Advanced DMEM/F12 medium was added supplemented with 100 µg/ml cycloheximide (Merck) to stop new protein synthesis and organoids incubated for the indicated release times at 37°C ...
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bioRxiv - Genetics 2023Quote: ... mouse anti-GS (1:500, Merck MAB302), rabbit anti-PCNA (1:400 ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were grown in 25% TSB medium at 28°C in static conditions in glass microcosms with one layer of ColiRoller glass beads (Millipore, Merck, USA) covering the base of the microcosm to provide additional spatial structure ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... and anti-Fas activating antibody (Merck) was diluted in 50% glycerol in PBS.
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bioRxiv - Cancer Biology 2023Quote: ... 0.4 mg/ml collagenase from Clostridium histolyticum type V (Merck), and ROCK inhibitor Y-27632 ...
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bioRxiv - Cancer Biology 2023Quote: Lysates from HEK293 cells overexpressing FLAG-tagged wild typeTP53INP2 or the TP53INP2-3A were affinity-purified with anti-FLAG-M2 affinity beads (Merck). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 nM SB202190 (Merck), 10 nM 17-β Estradiol (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ERK5 inhibitors JWG-071 (Merck), AX-15836 (MedChemExpress) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 μM Nicotinamide (Merck), 500 nM A83-01 (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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bioRxiv - Cancer Biology 2023Quote: ... Enzymatic digestion was done with 1.25 U/ml dispase II (Merck), 0.4 mg/ml collagenase from Clostridium histolyticum type V (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: ... the media were replaced with DMEM for Mass Spectrometry (Sigma-Aldrich, Merck Life Science, Milan, Italy) for MiaPaCa-2 and PANC1 cell lines ...
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bioRxiv - Cancer Biology 2023Quote: ... The HLA-peptide complexes were then eluted using 10% acetic acid and subsequently subjected to molecular weight cut-off filters (small scale) or fractionated using a reversed-phase C18 end capped HPLC column (Chromolith SpeedRod, Merck), running a mobile phase buffer A of 0.1% trifluoroacetic acid (TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... and BPTES were purchased from Sigma-Aldric (Merck Life Science, Milan, Italy), while erastin (ERA ...
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bioRxiv - Cancer Biology 2023Quote: ... U13C Glucose and unlabeled Glutamine or U13C Glutamine and unlabeled Glucose (Sigma-Aldrich, Merck Life Science, Milan, Italy) were also added ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 nM A83-01 (Merck) and N-acetyl-L-cysteine 1.25mM (Merck).
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (Merck) were diluted in H2O 0.1% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted proteins were pooled and concentrated using 10 kDa molecular weight cut-off centrifugal filters (Merck Millipore). The concentrated sample was loaded onto a HiLoad Superdex S200 (16/600 ...
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bioRxiv - Genomics 2023Quote: ... Slide that now contained stained cells was air dried and mounted with 1ml of 85% glycerol (Merck Millipore) and covered with a coverslip (Menzel-Glaser) ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Microbiology 2023Quote: ... 15 mL of the filtered culture supernatant was first concentrated using Amicon Ultra 15 mL Centrifugal Filters (cut-off 10 kDa, Merck), and the proteins were precipitated out of the solution using trichloroacetic acid (TCA ...
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bioRxiv - Molecular Biology 2023Quote: ... At 48 hours of transfection (If indicated) the cells were treated with the following conditions before harvesting at indicated times: 2mM of H2O2 (MERCK) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... GFP (MERCK, MAB2510), RPA32 (Cell Signaling 2208) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-phospho-Histone H2A.X (Ser139)(abcam ab 2893 and Merck Millipore, JBW301), phospho-RPA2 (Ser33 ...
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bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
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bioRxiv - Neuroscience 2023Quote: The viability of SH-SY5Y cells after indicated treatments was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) assay ...
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bioRxiv - Neuroscience 2023Quote: ... The fluorescent dye dihydroethidium (DHE) (Sigma-Aldrich; Merck KGaA) was used to detect the intracellular ROS levels ...