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Citations for Biotek :
201 - 237 of 237 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... All assays were allowed to incubate at 37°C for 30 min before protein concentration was read in at 562 nm (SynergyMix, Biotek).
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bioRxiv - Cancer Biology 2022Quote: ... Cell and tissue debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
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bioRxiv - Microbiology 2023Quote: ... The green fluorescent protein (GFP)-positive cells were then counted using Cytation 5 with Gen5 v3.03 software (BioTek, Winooski, VT). Dose-response curves were plotted and EC50 values determined using Prism 9 (GraphPad ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell debris was removed by centrifugation at 16,000x g for 5 minutes at room temperature and protein concentration was measured using A280 values determined by Cytation 5 (BioTek). Lysates were normalized in Buffer A with 1% SDS and prepared with 6X Laemmli sample buffer containing 100 mM 2-mercaptoethanol (Sigma M6250) ...
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bioRxiv - Physiology 2019Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Intra-assay CV was 4.44% and inter-plate CV was 5.94%.
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Samples from 3 females and 2 males initially showed greater than 80% maximum binding ...
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bioRxiv - Biochemistry 2023Quote: ... The xanthine/xanthine oxidase method based on the production of O2- anions was used to assay T-SOD activity by ELISA (Biotek, Vermont, America, λ= 450 nm). The liveness of T-SOD is shown as units per milligram of protein (U/mg protein).
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bioRxiv - Cancer Biology 2019Quote: ... DNA concentration was measured by SYBR green fluorescence with a λ dsDNA calibration curve (readings on BioTek Synergy H1). ClonTracer DNA barcodes consist of a repeating ‘Strong (G or C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both fragments were cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.) and then fused in an overlapping PCR to make an IS1 fragment lacking inverted repeats but having SspI sites on the ends and an XhoI site in the center ...
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bioRxiv - Microbiology 2022Quote: ... DNA was immediately extracted from a portion of the zooplankton tissue mass via the EZNA tissue kit (Omega Biotek) and was used to create a highly concentrated standard whose DNA concentration was measured with a Qubit 2.0 (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from swab or tissue supernatant using the Mag-Bind Viral DNA/RNA 96 Kit (Omega Biotek) on the KingFisher FLEX System (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The A260:A280 ratio and the isolated genomic DNA quantification were determined using a multimode plate reader (Biotek, India).
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bioRxiv - Bioengineering 2019Quote: ... emission at 517 nm) of the membrane and cytoplasmic proteins was determined using fluorescence spectrophotometer (Synergy H4; BioTek, Winooski, VT, USA).
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bioRxiv - Neuroscience 2021Quote: ... Plasma osmolality was analyzed by vapor pressure osmometry (VAPRO 5520) and volemia was indirectly inferred by the plasma protein concentration which was measured in an absorbance microplate reader (BioTek EL800), according to the protocol proposed by Lowry et al ...
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bioRxiv - Synthetic Biology 2022Quote: The fluorescence intensity of the synthesized fluorescent proteins was measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 µL of the cell-free synthesized fluorescent protein and 45 µL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Biophysics 2023Quote: Assessment of the extent of protein oligomerization was based on monitoring absorbance at 550 nm using BioTek Plate Reader (Agilent BioTek NEO2) to reflect turbidity changes induced by protein oligomer formation ...
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bioRxiv - Microbiology 2024Quote: ... fumarate) or flurometric (citrate, acetyl-CoA) assays (cat. no. KA1674, ab102516, KA3791 and MAK039, respectively) and a microplate reader (BioTek Synergy Neo2, Agilent, Santa Clara, CA) according to manufacturer’s instructions (Abnova ...
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bioRxiv - Biochemistry 2019Quote: Fluorescence anisotropy experiments were carried out at room temperature with 5 nM fluorescein (6FAM)-labelled DNA substrates (Table S2) using a Synergy 4 plate reader (BioTek). Titration with protein was performed by serially diluting protein in 40 μL of assay buffer (20 mM Tris Acetate pH 7.0 ...
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bioRxiv - Biochemistry 2020Quote: ... Oligos were converted to double-strand DNA using qPCR and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). The pSC101 backbone was amplified with two separate primer pairs encoding BsaI cut sites that matched the insertion location of the oligos on the TtgR gene ...
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bioRxiv - Biochemistry 2022Quote: ... Oligonucleotides were converted to double-stranded DNA using 15 cycles of PCR amplification and purified on a spin column (EZNA Cycle Pure kit from Omega BioTek). A pSC101 backbone containing extant E ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We then removed small DNA fragments using Serapure magnetic beads (Rohland & Reich 2012) or Omega magnetic beads (Omega Biotek, 2021) in a 0.8:1 ratio (beads ...
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bioRxiv - Bioengineering 2020Quote: ... Red fluorescent protein (RFP) readings (excitation 580 nm, emission 610 nm) were taken using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT) after 18 hours of incubation at 37 °C with 200 rpm shaking.
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Neuroscience 2024Quote: ... which used bovine serum albumin standards to create a best fit linear regression line based on protein concentrations using the 562nm absorbance mode of a microplate reader and its Gen5 Data Analysis program (Biotek, Winooski, VT). Equal amounts of protein were resolved in SDS-polyacrylamide gels and electrophoretically transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Zoology 2020Quote: ... we extracted DNA from the well-preserved core of one fecal pellet per sample (Menke, Meier, & Sommer, 2015) using the E.Z.N.A.® DNA isolation kit (Omega Biotek, Norcross, GA, U.S.). The DNA concentration and quality were evaluated using Nanodrop™ 2000 Spectrophotometer (Nanodrop ...
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bioRxiv - Immunology 2022Quote: ... Recombinant protein was checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA). NEU3 is 51 kDa ...
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bioRxiv - Immunology 2020Quote: ... Buffer-exchanged preparations were then checked for protein concentration by OD 260/280/320 using a Take3 micro-volume plate with a SynergyMX plate reader (BioTek, Winooski, VT, USA) as previously described (54) ...
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bioRxiv - Bioengineering 2020Quote: The DNA concentration in the supernatant was measured by UV spectrometry at a wavelength of 260 nm (BioTek, Gen5 plate reader, USA). 96-well UV flat-bottom plates (Greiner UV Star 96 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Both products were DpnI digested and cleaned with the Viogene Gel/PCR DNA Isolation Kit (Viogene-Biotek Corporation, New Taipei, Taiwan R.O.C.). These fragments were assembled in a Circular Polymerase Extension Cloning (CPEC ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Quantification of the extracted DNA was conducted spectrophotometrically with the Take 3 plate in an Eon plate reader/incubator (BioTek, Winooski, VT).
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bioRxiv - Microbiology 2024Quote: ... DNA amplification protocol was described previously (51) with the following exceptions: Mag-Bind RxnPure Plus magnetic beads solution (Omega Biotek, Norcross, GA, USA) was used instead of Mag-Bind E-Z Pure ...
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bioRxiv - Genomics 2020Quote: ... Pure agar cultures were grown to early log phase and harvested prior to gDNA extraction using the Omega Metagenomics DNA kit (Omega BioTek Norcross GA, M5633-00). Briefly ...