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Citations for Biotek :
151 - 200 of 237 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... The viability and cytotoxicity of the groups were evaluated using Calcein/PI assay and measured with a microplate reader (H1M, BioTek, USA).
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bioRxiv - Bioengineering 2021Quote: ... The proliferation activities of the cells in the groups were evaluated using the MTT assay and measured with a microplate reader (H1M, BioTek, USA).
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bioRxiv - Molecular Biology 2021Quote: ... the firefly LUC and Renilla LUC activities were measured by Dual Luciferase Reporter Gene Assay Kit (Yeasen, Shanghai) and Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, USA). Activity is expressed as the ratio of firefly LUC activity to Renilla LUC activity ...
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bioRxiv - Biochemistry 2024Quote: ... The assay was performed at 24°C using a Synergy HTX multi-mode reader (ex/em = 360 nm/460 nm; BioTek Instruments). Fractions containing proteasome activity were pooled and loaded onto a 5 mL anion exchange HiTrap DEAE FF column (Sigma) ...
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bioRxiv - Biochemistry 2021Quote: ... The absorbance of the concentrated protein was taken at 280 nm using Synergy H4 Hybrid Reader (BioTek) on a Take3 plate ...
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bioRxiv - Bioengineering 2024Quote: ... Approximately 20 ug of protein from each sample was resolved on a 4-20% gradient gel (Biotek), then visualized by SimplyBlue SafeStain.
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bioRxiv - Evolutionary Biology 2019Quote: ... The protein concentration of tissue homogenate supernatant and plasma samples was measured prior to the assay using a Biotek Take3 protocol (Biotek, Vermont, USA) and samples were diluted in PBS buffer to give a protein range between 1-10 mg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... and the fluorescence intensity was measured at the end of the assay using a microplate reader (BioTek, Ex/Em = 350/440 nm).
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bioRxiv - Microbiology 2021Quote: ... The absorbance of formazan at 490 nm is detected using an enzyme-linked immunosorbent assay (ELISA) plate reader (ELx808, BioTek Instruments, Inc.). Each measure was performed in triplicate.
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bioRxiv - Microbiology 2021Quote: ... The quantity of DNA in each sample was fluorescently measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) using a Synergy H1 Microplate reader (BioTek, Cat # BTH1M).
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bioRxiv - Microbiology 2022Quote: ... The quantity of DNA in each sample was measured with the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFicher, Cat# P11496) read with a Synergy H1 Microplate reader (BioTek, Cat # BTH1M). Sequence libraries were prepared from the extracted DNA samples using the Nextera DNA Flex Library Prep Kit (Illumina ...
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bioRxiv - Biochemistry 2021Quote: ... The relative activities of the firefly luciferase and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... four days after transfection and luciferase activity was measured using the Nano-Glo® Dual-Luciferase® Reporter Assay System using the Synergy Neo2 multi-mode reader (BioTek). Nanoluciferase activity was normalized to that of the firefly luciferase.
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bioRxiv - Immunology 2022Quote: ... and luminescence in relative light units (RLUs) was measured using a Synergy Neo2 Multi Mode Assay Microplate Reader (Biotek Instruments, Winooski, VT). Three biological replicates were performed for each antibody or MB ...
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bioRxiv - Biochemistry 2023Quote: ... The color development in each assay well was monitored at a series of time points using an automated microplate reader (BioTek, Synergy-HTX). A420nm values in the linear course of color development were used to plot titration curves and the antibody dilution required for 50% maximum binding was quantify for the binding affinity of mAb TnI-1 to its specific epitope in the TnT constructs ...
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant was measured using an iron assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.).
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bioRxiv - Cell Biology 2022Quote: ... high g pellet and high g supernatant using a lipid hydroperoxide assay kit and Synergy Mx Multi-Mode Microplate Reader (BioTek Instruments Inc.). For the iron depletion assay ...
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bioRxiv - Biochemistry 2023Quote: ... Relative activities of the firefly and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitation and purity check of isolated DNA was done using spectrophotometer (BioTek EPOCH|2 microplate reader). DNA was extracted from young leaves using a standard CTAB protocol and the samples were diluted with with 1XTE to 50 ng/μl for PCR reactions.
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bioRxiv - Synthetic Biology 2019Quote: ... Plasmids were isolated from subcultures using a DNA miniprep kit following the manufacturer’s instructions (Omega BioTek) and were Sanger sequenced to identify correctly assembled plasmids.
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bioRxiv - Microbiology 2023Quote: ... was used to remove DNA contaminants and the RNA was quantified on an Eon spectrophotometer (BioTek). For the imipenem induction experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... The plasmid DNA isolated was quantified using the spectrophotometer EPOCH2 microplate reader (BioTek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2024Quote: ... The plasmid DNA isolated was quantified using the spectrophotometer EPOCH2 microplate reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2021Quote: ... Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Cell Biology 2022Quote: ... 0.5 mM DTT) into the protein mix using the automated dispenser of a Synergy 4 microplate reader (BioTek) to a final volume of 200 μl and a final concentration of 2 μM actin in 1x KMEI with 0.05% antifoam ...
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bioRxiv - Bioengineering 2020Quote: ... Reactions for the fluorescent proteins were carried out in a Synergy Neo2 HTS Multi-Mode Microplate Reader (BioTek) at 282 cpm.
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bioRxiv - Immunology 2020Quote: ... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
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bioRxiv - Biophysics 2022Quote: In vivo and in vitro spectra of all proteins were recorded using Synergy H4 Hybrid Microplate Reader (BioTek) with resolution of 9 nm ...
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bioRxiv - Molecular Biology 2023Quote: ... and the total protein in each extract was estimated by reading the OD280nm using the spectrophotometer (Epoch, BioTek). For the Western blot ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT) and 1 μg was boiled for 10 min in 1x Laemmli Sample Buffer (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein was quantified by absorbance at wavelength 280 nm using a Take3 microvolume plate (BioTek Synergy HT). In assay buffer ...
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bioRxiv - Microbiology 2020Quote: ... Cells were fixed in 3.7% formaldehyde and the assay was read on Lionheart FX automated fluorescent microscope (BioTek Instruments, Inc., Winooski, VT, USA). The total number of cells per well was counted using the Object Count feature in Nikon Elements AR Analysis version 5.02 by a blinded observer ...
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bioRxiv - Microbiology 2022Quote: ... The growth curve assays were performed in non-treated non-tissue culture plates using a plate reader (BioTek Synergy neo2 multi mode reader) in 200μl of 7H9 medium at 37°C starting at an Optical Density at 600nm (OD600 ...
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bioRxiv - Systems Biology 2019Quote: ... Assay plates were incubated for 24 h (30°C, 160 rpm) in a Cytation3 cell imaging multimode reader (BioTek Instruments, Winooski, VT, USA). At 24 h ...
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bioRxiv - Genetics 2020Quote: ... Luciferase activity was determined by analyzing 50 μl of each cell extract in a luciferase assay system according to the standard protocol for the Synergy 2 Multi-Mode Microplate Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Cell Biology 2023Quote: ... 100 µl of the medium of the upper compartment was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Bioengineering 2023Quote: ... 100 µl of the medium was pipetted in a black 96-wells assay plate and fluorescence was measured with a plate reader (SynergyTM HTX, Biotek, Winooski, VT, USA) at excitation 530/25 nm ...
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bioRxiv - Microbiology 2023Quote: ... All endpoint assays and growth curves were carried out at 30°C and 548 rpm using a Synergy HTX plate reader (BioTek, Winooski, VT, USA) with OD600 readings measured every 30 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... genomic DNA was also extracted from the pool of transfected cells using an E.Z.N.A Tissue DNA Kit (Omega Biotek). Translocation frequencies were estimated by serial dilution as previously described (Renouf et al. ...
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bioRxiv - Genomics 2022Quote: Cell-free DNA was extracted from plasma using the Magbind ccfDNA extraction kit (Omega Biotek cat#M3298). Manufacturer recommendations for extraction were followed ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA concentration and purity were evaluated by calculating the A260/280nm ratio using a spectrophotometer (Epoch, BioTek). DNA integrity was verified on 1% agarose gel.
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bioRxiv - Microbiology 2023Quote: ... and the DNA was selected for fragments above 300 bp using Totalpure NGS mag-bind (Omega-BioTek) at 0.85x ratio (beads to DNA volume) ...
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bioRxiv - Biochemistry 2020Quote: ... Low-affinity complexes and unbound proteins were removed in consecutive washing steps with PBS-T 0.05% (EL406 washer, BioTek). Beads were incubated for 10 minutes with 0.4% paraformaldehyde ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we performed automated growth-assays on each of the 24 revived populations using a well-plate reader in multiple environments (Synergy HT, BIOTEK ® Winooski, VT, USA). Using optical density at 600 nm as the measure of population density ...
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Microbiology 2022Quote: ... Extracted DNA from each sample is quantified in 384 well plates on a fluorescent plate reader (BioTek Neo2) using the Quant-iT PicoGreen assay (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was isolated from the tissue samples of Þistilfjörður population using the E-Z 96 Tissue DNA Kit (Omega Biotek) according to the manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2019Quote: ... and the fluorescence intensity of Venus-tagged protein was measured on a Synergy H4 Hybrid reader (Biotek, Winooskit, VT, USA) at excitation and emission wavelengths of 485 nm and 528 nm ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by taking OD at 280 nm in Nano drop (Synergy H1 Hybrid Multi-Mode Reader Biotek) and mass extinction co-efficient of the proteins.
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bioRxiv - Microbiology 2020Quote: The TreS activity domain of TreS-Mak protein was monitored following the conversion of trehalose into maltose and glucose using Epoch spectrophotometer (Biotek). 15µL of reaction sample were incubated 30 min at 58°C with 30 µL of 100 mM sodium citrate pH 4.6 containing whether 0.4 U of amyloglucosidase from Aspergillus niger (Megazyme ...