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Citations for Biotek :
101 - 150 of 237 citations for Protein DNA Interaction Assay since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... after genome extraction from larva or adults (E.Z.N.A insect DNA kit, Omega Biotek).
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bioRxiv - Zoology 2021Quote: ... DNA was quantified using a spectrophotometer (Epoch, BioTek Instruments, Inc., Winooski, VT, USA). In the case of low DNA yield from the MAXI extraction (<85 ng/μL) ...
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bioRxiv - Bioengineering 2023Quote: ... and purified by DNA purification and gel extraction kits (Omega Biotek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Molecular Biology 2021Quote: The dual-luciferase assay was performed using a Cytation™ 5 plate reader with a dual-injector system (BioTek). For each sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Cellular abundance was read out 72 hours later using the Presto Blue assay (Thermo) on a Cytation 3 (BioTek) plate reader ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... reduction assay at a concentration of 1 mM and subsequently measured by spectrophotometer (Epoch, BioTek Instruments, Winooski, VT, USA), at 570nm and 600nm wavelengths [27] ...
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bioRxiv - Physiology 2019Quote: ... in ng·mL-1) were assessed via enzyme-linked immunosorbent assays (ELISA) via a 96-well spectrophotometer (BioTek, Winooski, VT) using commercially available kits ...
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bioRxiv - Cell Biology 2021Quote: ... to measure luminescence in relative light units (RLUs) using a Synergy Neo2 Multi-Mode Assay Microplate Reader (Biotek Instruments).
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bioRxiv - Molecular Biology 2022Quote: ... was used to assay growth of the organoids according to the manufacturer’s instruction and luminescence was measured using Synergy HTX (BioTek).
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bioRxiv - Systems Biology 2023Quote: ... The high-throughput growth assays were performed using the 96-well microplates (Costar) and the plate reader (Epoch2, BioTek), as described in the previous studies [28] ...
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bioRxiv - Microbiology 2023Quote: ... Assay plates were incubated at 30 °C with 548 rpm with a 2 mM orbital in a Neo2SM (BioTek) or Synergy H1(BioTek ...
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bioRxiv - Physiology 2023Quote: ... The colorimetric assay was conducted in a 96-well plate and absorbance was measured using a plate reader (BioTek) at 570nm ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was determined using the BCA assay according to the manufacturer’s instructions and measured on a Powerwave XS plate reader (Biotek). Counts were normalized to protein concentration ...
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bioRxiv - Physiology 2022Quote: ... protein concentration was determined by absorbance at 450 nm in a plate reader (Biotek Instrument).
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bioRxiv - Immunology 2020Quote: ... Protein quantification was carried out after thawing in a Synergy HTX Multi-Mode Reader (Biotek).
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bioRxiv - Plant Biology 2022Quote: ... with a BSA range and protein concentration was quantified with a ELx808 microplate reader (Biotek). Enzymatic activity was measured via the derived products generated from a 4-MUG substrate (M1404 ...
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bioRxiv - Plant Biology 2020Quote: ... DNA quantity and quality was evaluated using a microplate reader Synergy HT (Biotek, Germany), using the software Gen5™ (Biotek ...
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bioRxiv - Bioengineering 2019Quote: ... and the DNA concentration is measured using an Epoch™ Microplate Spectrophotometer (BioTek™).
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bioRxiv - Systems Biology 2020Quote: ... and magnetic bead-compatible reagents (Mag-Bind Blood and Tissue DNA HDQ, Omega Biotek). Purified gDNA was eluted in 10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Plant Biology 2021Quote: ... DNA concentration was measured using a Synergy LX plate reader (BioTek, Winooski, VT, USA). Quantification of CLas in plant tissues was performed as described elsewhere 6 ...
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bioRxiv - Synthetic Biology 2022Quote: ... and then purified by DNA purification and gel extraction kits (Omega BioTek, GA, USA). For the ligation-dependent cloning ...
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bioRxiv - Cancer Biology 2023Quote: ... to digest any residual chromosomal DNA and then quantified using a microplate spectrophotometer (BioTek Epoch Microplate Spectrophotometer ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was quantified using a Take3 Microvolume Plate Spectrophotometer (BioTek, Winooski, VT, USA). Whole-genome sequencing via PacBio Sequel II was performed by the Department of Energy’s Joint Genome Institute (JGI ...
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bioRxiv - Biochemistry 2021Quote: Proteins either in 0.02% DDM/0.004% w:w DDM/CHS detergent or reconstituted in 80:20 w:w BPL/cholesterol nanodiscs and liposomes were used in molybdate based calorimetric ATPase assay (42) measured at 850 nm on a SYNERGY Neo 2 Multi-Mode microplate reader (BioTek). The assay was carried out at 37°C in a 40-µl volume in 96-well plates ...
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bioRxiv - Biochemistry 2021Quote: ... Aliquots of cell lysates and media were then transferred to assay plates and GFP fluorescence was quantified by fluorometry on a fluorescence microplate reader (Ex 470, Em 510; BioTek). After subtraction of background values ...
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bioRxiv - Neuroscience 2021Quote: ... Optical density of the Enzyme Linked Immunosorbent Assay (ELISA) was measured at 450 nm using a Synergy HTX multi-mode microplate reader (Biotek) and compared to a standard curve to determine the final concentration ...
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bioRxiv - Neuroscience 2020Quote: ... The rats’ insulin levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (Hangzhou Eastbiopharm Co., Ltd., Hangzhou, China) and a spectrophotometer (Biotek Epoch™ Microplate Spectrophotometer ...
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bioRxiv - Microbiology 2020Quote: ... or human IFN-β (1000 U/ml) and gaussia luciferase activity was determined using Gaussia Luciferase Glow Assay Kit (Thermo) on Cytation 3 (BioTek) multimode plate imager according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Chloride channel conductance recordings were measured at 30 s intervals on the Synergy Neo2 Multi-Mode Assay Microplate Reader (BioTek). Following 5 min of baseline recording ...
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bioRxiv - Cell Biology 2022Quote: ... Methylation levels for both assays were established by reading absorbance at A450 nm via a microplate reader (BioTek, Synergy 2) using program Gen5 1.10 for quantification via provided standards.
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bioRxiv - Synthetic Biology 2020Quote: ... The supernatant of each plate was then carefully discarded and cell viability determined by crystal violet assay reading the absorbance at 595 nm (Synergy HTX Biotek) as described41.
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bioRxiv - Microbiology 2020Quote: ... tissue culture supernatants were collected at 48 h post-infection and Nluc expression was measured using a luciferase assay and a Synergy LX microplate reader (BioTek). Individual wells from three independent experiments conducted in quadruplicates were used to calculate the average and standard deviation (SD ...
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bioRxiv - Microbiology 2020Quote: ... tissue culture supernatants were collected at 48 h post-infection and Nluc expression was measured using a luciferase assay and a Synergy LX microplate reader (BioTek). Individual wells from three independent experiments conducted in quadruplicates were used to calculate the average and standard deviation (SD ...
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bioRxiv - Cancer Biology 2020Quote: ... The number of cells was determined at the end of the run using the SRB assay as previously described (62) or in plate cell counting by Cytation 1 imaging reader (Biotek).
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bioRxiv - Cell Biology 2022Quote: ... The plate was then cultured for 1 day before washing with assay buffer (1x HBSS, 10 mM HEPES, pH 7.4) in a ELx405 CW plate washer (BioTek Instruments). The cells were then incubated with 1.25 µM calcium indicator Fluo-8 AM (AAT Bioquest ...
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bioRxiv - Microbiology 2022Quote: ... Batch growth kinetic assays were performed in 96-well plates and growth was measured using a micro-well plate reader (Biotek). For this ...
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bioRxiv - Pathology 2023Quote: ... The assay was performed at 37°C in 96-well format and the fluorescence recorded using Synergy H1 plate reader (BioTek). Mir05 buffer with 1 mM malate ...
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bioRxiv - Cell Biology 2022Quote: ... Lipid hydroperoxides were extracted from neuronal lysates and neuron-conditioned media and analyzed using a lipid hydroperoxide assay kit according to the manufacturers protocol and Synergy Mx Multi-Mode Microplate Reader (BioTek). VAMP7 and syntaxin 4 knockdown neurons used for the lipid hydroperoxide assay were transduced at an MOI 7.5.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cells were washed with Assay Buffer before being photographed and determining a double fluorometric measurement using a microplate fluorescence reader (Synergy HT BioTek). The autophagosomes were quantified by normalizing green fluorescence (Cyto-ID ...
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bioRxiv - Biophysics 2023Quote: ... or mVenusH148Q/I152L and mTq2 (for I-/Cl- antiport assay) were measured in a time dependent manner using Synergy Neo2 (Agilent/BioTek) and the data analyzed offline as described previously (Wasano et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Supernatant was collected and fluorogenic assay was performed in a plate reader (Synergy HT Plate Reader (BioTek, Winooski, VT, USA). GUS assay buffer was pre-warmed at 37°C and 25 µl of plant extract (supernatant from sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Assay plates were measured for the formazan product at 490 nM using a Synergy 2 plate reader (BioTek, Winooski, VT). Net lactate production was determined by subtracting lactate in the initial media from cancer cell’s lactate production at each time point during the experiments.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA concentration measurements were performed using the Qubit dsDNA high sensitivity assay kit on the Synergy H1 Hybrid Microplate Reader (BioTek). Libraries were generated using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Protein content was measured using 260/280 absorbance ratio with Take3 Micro-Volume Plate reader (BioTek)
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bioRxiv - Plant Biology 2022Quote: ... Protein concentration was estimated using a Synergy H1 microplate reader (BioTek Instruments, Inc., Winooski, VT, USA) with a Take3 Micro-Volume Plate (BioTek Instruments ...
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bioRxiv - Microbiology 2021Quote: ... The purified DNA samples were quantified on a microplate reader (Synergy LX, BioTek, Winooski, VT), and 500 ng of each DNA sample (20 ng/µl ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were purified from positive DH5α colonies with ENZA plasmid DNA mini kit (Omega Biotek) and submitted to Genewiz for sequencing ...
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bioRxiv - Systems Biology 2021Quote: Vmax assays for comparison of yeast and human cell extracts were carried out with freshly prepared extracts via NAD(P)H-linked assays at 37 °C in a Synergy H4 plate reader (BioTek™). The reported Vmax values represent total capacity of all isoenzymes in the cell at saturating concentrations of all substrates and expressed per extracted cell protein ...
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bioRxiv - Bioengineering 2022Quote: ... Mineral precipitation in the mineralization solution was determined by measuring the optical density of 100 μl sample in a 96-wells assay plate at 600 nm using a plate reader (Synergy™ HTX, Biotek).
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bioRxiv - Systems Biology 2022Quote: ... All assays were performed in triplicate wells at 25°C in a Synergy HTX Multi-Mode Microplate Reader (BioTek, Winooski, VT) with excitation and emission wavelengths of 360 and 460 nm ...