Labshake search
Citations for Biotek :
2401 - 2450 of 2549 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... Quantification was performed by measuring soluble A600 CV absorbance using a Biotek Synergy HT microplate reader and Gen 5 software (Biotek, Winooski, VT). Values were normalized to culture growth by dividing by final culture OD600.
-
bioRxiv - Plant Biology 2022Quote: ... The DNA was normalized to 5 ng/μL based on quantification with a Synergy 2 multimode microplate reader (Biotek Instruments, Winooski, VT) with the Quant-iT PicoGreen double-stranded DNA quantification assay (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2022Quote: ... and day 2 by addition of the CTG reagent followed by measurement of luminescence using the Biotek Cytation 5 (BioTek, Winooski, VT). Luminescence values were adjusted based on 2µM Adenosine triphosphate (ATP ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... The O.D./min was continuously recorded at 405 nm over 30 min to measure the hydrolytic product 5-mercapto-2-nitrobenzoic acid using a Synergy H1 microplate reader (Biotek; VT, USA). O.D./min was converted to catalytic rate using the Beer-Lambert law:
-
bioRxiv - Microbiology 2023Quote: ... The reaction was stopped by adding 50 μl of stop solution and the absorbance was recorded at 450nm using Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Microbiology 2023Quote: ... Plasmin activity was monitored by continuously measuring the rate of change of absorbance at 405 nm for 20 minutes using Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Microbiology 2023Quote: GFP or mCherry-positive cells were imaged on the Cytation 5 imaging reader and masked using the accompanying Gen5 software (BioTek Instruments, Inc.). The final cell-to-cell spread was calculated as GFP or mCherry-positive cells per area of the entire well ...
-
bioRxiv - Bioengineering 2023Quote: ... was quantified by measuring the absorption at 620 nm using a UV/Vis-spectrophotometer (Cytation 5 imaging reader: BioTek, Winooski, VT, USA). A standard curve was used to correlate the absorption value with the amount of HA ...
-
bioRxiv - Plant Biology 2023Quote: ... The background luminescence of seedling was recorded for 2 min before application of flg22 and chitin (5 s for NaCl) on a microplate reader (BioTek Synergy H1). Then 50 μl of 200 nM flg22 ...
-
bioRxiv - Microbiology 2023Quote: ... was recorded every 1 min over a period of 4 hours at 37°C using the Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Microbiology 2023Quote: ... 2N H2SO4 solution was added to stop the reaction and absorbance was measured at 450 nm using Cytation 5 microplate reader (BioTek Instruments, USA).
-
bioRxiv - Immunology 2020Quote: ... Plasmids were purified using the EZNA plasmid maxi kit (Omega BioTek), according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: ... and DNA purification and gel extraction kits (Omega Biotek, GA, USA) were used to amplify DNA fragments and purify the PCR products ...
-
bioRxiv - Microbiology 2023Quote: ... followed by extraction using E.Z.N.A Viral RNA Kit (R6874, Omega Biotek). Then ...
-
bioRxiv - Plant Biology 2023Quote: ... The RNA-prep pure PLANT Kit (DP432, Tiangen Biotek, Beijing, China) was used to isolate total RNA from the rice grains ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are then purified using EZNA Cycle Pure kits (Omega BioTek). Isothermal assembly followed Gibson Assembly protocols (NEB) ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA extraction was performed using E.Z.N.A Total RNA kit (Omega BioTek) following manufacturer directions ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed in 4% PFA and stained with Hoechst nuclear stain before GFP-positive cells were counted using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) with the onboard software for calculations of percentage infected cells.
-
bioRxiv - Microbiology 2020Quote: ... Infected cells were then maintained at 37°C for 14-16 h post infection before automatic counting of GFP-positive cells using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, Winooski, VT) and using the onboard software for calculations of percentage infected cells.
-
bioRxiv - Cancer Biology 2021Quote: ... at 1.5×104 cells/ well in 50 μl of DMEM supplemented with 10% FBS using BioTek™ MultiFlo™ FX Microplate Dispensers (BioTek Instruments). Cells were treated with an FDA approved library (Selleckchem ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... TuCPR activity was measured as the reduction of cytochrome c by calculating absorbance at 550 nm over 5 minutes by with the kinetic mode in a Synergy H1 Hybrid Multi-Mode Reader microplate reader (Biotek, Winooski, VT, USA). The control reaction was the non-enzymatic reduction of cytochrome under the same conditions ...
-
bioRxiv - Plant Biology 2020Quote: ... Fluorescence intensity was measured at the vascular bundles of leaves 5 mm from the detachment sight using the Lionheart FX Gen5 image analysis mode (BioTek, Winnoski, VT, USA). Untreated plants were used as control with 6 biological repeats ...
-
bioRxiv - Microbiology 2020Quote: ... RNA concentration and quality were measured using a biophotometer and on multimode spot reader using a Cytation™ 5 (BioTek Inc. Winooski, VT). Total RNA from different samples were used to synthesize cDNA using Superscript II reverse transcription kit (Invitrogen ...
-
bioRxiv - Neuroscience 2020Quote: ... The neurons were transferred to an automated live-cell incubator/imaging system (BioSpa™ 8 Automated Incubator coupled with a Cytation™ 5 Cell Imaging Multi-Mode Reader, BioTek, USA). The plates were incubated in the BioSpa at 37°C and 5% CO2 for 4 days ...
-
bioRxiv - Cancer Biology 2020Quote: ... The plates were then incubated for 1 hour at 37 °C and 5% CO2 and then absorbance at 450 nm was measured using a Synergy H1 Hybrid Multi-Mode Microplate Reader (Biotek, Winooski, VT, USA).
-
bioRxiv - Cell Biology 2020Quote: ... neurons were stored in an automated incubator/imaging system (Cytation™ 5 Cell Imaging Multi-Mode Reader associated with a BioSpa™ 8 Automated Incubator, BioTek, USA). The plates were stored in the BioSpa at 37°C and 5% CO2 and were automatically transferred to the Cytation™ 5 for imaging ...
-
bioRxiv - Cell Biology 2021Quote: ... isopropyl alcohol to quantitatively measure the density of the stain in different groups at 520 nm using microplate reader (Gene 5 Bio Tek Spectrometer, BioTek, northern Vermont, USA). Isopropanol was used as a blank (26)
-
bioRxiv - Neuroscience 2020Quote: ... Immunofluorescence images were acquired using a Cytation 5 Cell Imaging Multi-Mode Reader and analyzed with Gen5™ software (BioTek, Winooski, VT, USA).
-
bioRxiv - Microbiology 2021Quote: ... the cells were collected by centrifugation at 10,000 g for 5 min and the absorbance of the supernatant was measured at 562 nm with a Synergy 2 spectrophotometer (BioTek, Winooski, VT, USA). Quantities of ferrihydrite reduced were determined using a standard curve containing a 2-fold range of FeSO4 (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... was measured every 5 min for 2 h at 25°C using Synergy H1 Hybrid Multi-Mode Microplate Reader (BioTek, cat. no. H1MFDG). The rate of kinetic reaction (slope ...
-
bioRxiv - Cancer Biology 2022Quote: ... The fraction of cells surviving was quantified using either a MacsQuant Analyzer 10 (Milltenyi Biotec, Germany) or by the Cytation 5 system (BioTek, Santa Clara, California). Cell viability was measured according to the manufacturer’s instructions for both instruments ...
-
bioRxiv - Biophysics 2022Quote: ... 100 μL of CellTiter-Glo 2.0 was added to each well and luminescence signal was measured in a Cytation 5 imaging reader (BioTek instruments Inc., Winooske, USA) after 10 min incubation.
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
-
bioRxiv - Neuroscience 2023Quote: ... and protein concentrations were determined using the Bradford Assay and an Epoch BioTek Microplate Spectrophotometer (Gen 5 2.01; BioTek Instruments Inc; Winooski, VT). 25 μg of protein from tissue homogenates were combined with specific cytokine antibody-immobilized beads ...
-
bioRxiv - Bioengineering 2023Quote: ... The fluorescence emission of each well was measured at 593 nm (20 nm bandwidth) with an excitation at 554 nm (18 nm bandwidth) using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments SAS, France). Each condition was replicated three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... was supplied by gas controller into the incubator of Cytation 5 multi-mode reader system equilibrated at 37 °C (BioTek; Winooski, VT, USA).
-
bioRxiv - Cancer Biology 2023Quote: Fluorescence intensity and absorbance measurements were carried out with Synergy NEO or Cytation 5 multi-mode readers (both from BioTek; Winooski, VT, USA). Bright-field and fluorescence microscopy was carried out with Cytation 5 multi-mode reader using 4× air objective (1.613 µm/pixel ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 24 h at 37 °C in the presence of 5% CO2.with an automated liquid dispenser (BioTek MicroFlo; Thermo-Fisher, Inc.) Drugs/chemicals were dosed in DMSO at 12 concentrations ranging from 50 pM to 50 μM (half-log dilutions ...
-
bioRxiv - Microbiology 2023Quote: ... The fluorescence intensity of FM4-64 was measured at 515/20 nm excitation and 635/20 nm emission wavelength using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, United States). A non-coated plate was used with EVs as a control.
-
bioRxiv - Microbiology 2024Quote: ... All samples were mixed via pipetting before fluorescence was read at 490ex/535em and 440ex/535em after 5 minutes (Biotek SynergyMx with Gen5 software). Background fluorescence from buffer alone was subtracted from each sample before calculating the ratiometric fluorescence of the calibration curve samples and experimental samples ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The amplicon was then purified using a Cycle Pure Kit (Omega Biotek). The vector backbone fragment was amplified from pET28a-M-CaM-cTdTS plasmid DNA constructed above using PfuUltra II Hotstart PCR Master Mix using DNA primers P29 – P36 listed in Table 1 ...
-
bioRxiv - Cell Biology 2022Quote: ... and DNase-treated using commercially available kits (Omega Biotek and Ambion, respectively). Libraries were constructed and subsequently subjected to 151bp paired-end cycles on the NovaSeq-6000 platform (Illumina) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... PCR product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The product was subsequently cleaned up using PCR cleanup kit (Omega Biotek) and stored in dH2O ...
-
bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using an Omega total RNA extraction kit (Omega Biotek) (column-based system ...
-
bioRxiv - Bioengineering 2020Quote: ... 5% CO2 using an automated digital microscope with a 10× objective with a gas controller (Cytation 3 microplate reader, BioTek Instruments Inc., Winooski, VT, USA). Images were collected every 10 minutes for 24h ...
-
bioRxiv - Neuroscience 2021Quote: ... and the EZNA MicroElute Total RNA kit (Omega BioTek, Norcross, GA; #R6831-01) with a DNase step (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... after genome extraction from larva or adults (E.Z.N.A insect DNA kit, Omega Biotek).
-
bioRxiv - Synthetic Biology 2021Quote: ... The approximately 8100bp amplicon was purified using a gel extraction kit (Omega Biotek). DNA concentration of both linear fragments was measured using the Take3 plate coupled with a Biotek Cytation 5 plate reader ...