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Citations for Biotek :
2151 - 2200 of 2549 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... of the wild-type and mutant strains in 96 well microtiter plates (Surface Nunclon ™ Roskilde, Denmark) using a Sinergy H1 Hybrid Multi-Mode microplate reader (Biotek, USA). For this ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated in a 96-well flat-bottom plate (Nunclon) at 37°C with continuous shaking (double orbital) in a microplate spectrophotometer (BioTek Epoch 2). Optical densities were recorded every 20 min ...
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bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
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bioRxiv - Microbiology 2024Quote: ... The growth behavior of the different mutants of AB5075-VUB was assessed in liquid culture using the Cytation 1 plate reader (BioTek, United States). Each well of a 96-well plate was filled with 200 µL of bacterial culture diluted to an initial OD600 of 0.1 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Neuroscience 2024Quote: ... was measured in cell culture medium collected from the top and bottom chamber of the Transwell system (three technical replicates per Transwell) using a fluorescent plate reader (Biotek Synergy H4) and clearance volume calculated as previously described (37,38).
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bioRxiv - Microbiology 2023Quote: ... plates were briefly centrifuged at 500 xg and absorbance at 620 nm was then measured using a Cytation7 plate reader (Biotek, Winooski, VT). Raw absorbance values of the compounds were normalized to DMSO (0% inhibition ...
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bioRxiv - Microbiology 2023Quote: ... The resulting 1,472 SICs were arranged in 16 96-well plates and incubated in the anaerobic chamber at 37 °C (by setting the temperature of the chamber to 37 °C) in a plate stacker (BioTek BioStack 4) with 2 control plates containing uninoculated media at the top and bottom of the stack to minimize edge effects ...
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bioRxiv - Microbiology 2023Quote: ... The 96-well plates were incubated and continuously recorded using a Synergy H1 Hybrid Multi-Mode Reader (BioTek Instruments, Inc., Vermont, USA) for 30h at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... staining and washing steps (2x, wash volume 50 µl) were performed using automated liquid handling systems (405 plate washer, BioTek– Tempest, Formulatrix).
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated shaking at 37°C and the OD600 was recorded every 15 min for eight hours in a 96-well plate reader (Biotek Synergy Neo2). Cells producing HT-DivIB ...
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bioRxiv - Molecular Biology 2023Quote: ... 200nM substrates (unmodified or preubiquitylated) was mixed in and the extract was incubated at 30C on a plate reader (Biotek Synergy H1). Signal was detected every minute by a build-in monochromator (ex ...
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bioRxiv - Synthetic Biology 2023Quote: The relative fluorescence units (RFU) of the synthesized fluorescent proteins were measured by the multi-well plate fluorometer (Synergy HTX, BioTek, Winooski, VT). 5 μL of the cell-free synthesized fluorescent protein and 45 μL of Milli-Q water were mixed in a 96-well half-area black plate (Corning Incorporated ...
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bioRxiv - Immunology 2023Quote: ... This was achieved by collecting samples from the serosal compartments every 30 minutes for 2 hours and measuring the respective FITC concentrations on a plate reader (Synergy H1; BioTek, Winooski, VT). Samples with visible FITC leakage in the serosal compartment were omitted ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris was used to extract protein-bound dye and optical density was measured with a BioTek Synergy HT plate reader (SN 269140, BioTek Instruments Inc.) at 490 and 540 nm ...
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bioRxiv - Microbiology 2023Quote: Analysis of the library of CRISPRi conditional mutants (derived from strain BCBMS14) was performed in a 96-well plate reader (Biotek Synergy Neo2). Overnight cultures were diluted 1:1000 in fresh media containing 10 µg/ml Cm ...
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bioRxiv - Biochemistry 2023Quote: ... Relative activities of the firefly and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek, Winooski, VT) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... was added according to the manufacturer’s instructions and the total luminescence was measured for 20 min using Synergy Neo plate reader (BioTek, New Castle, DE). Then agonists (10 μM carbachol (carbamoylcholine ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μg/ml) and grown for 20 hours before measuring optical density (OD) using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT). We also conducted experiments with the E ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of GFP (excitation 485 nm/ emission 528 nm) and dTomato (excitation 555 nm/ emission 585 nm) fluorescence using a BioTek Synergy plate reader (BioTek, Winooski, VT). Transformants with at least 3-fold increase in GFP expression were selected as candidate Zn reporter strains.
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bioRxiv - Biochemistry 2023Quote: ... Reactions were started by addition of 1 mM Mg-ATP and NADH absorbance was monitored at 340 nm in a plate reader (Biotek PowerWave HT) at 26°C ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Cell Biology 2023Quote: ... for spectrofluorometric analysis at 490 nm excitation/520 nm emission using a fluorescent plate reader (Biotek Synergy H4 or Biotek Synergy Neo2).
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken at 300 rpm (orbital) for 3 min and BRET biosensor performance was recorded using the plate reader’s luminescence filter (BioTek Synergy H1, v2.04.11) with an integration time of 1 s ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Hoechst staining a was read using an excitation of 392 nm and emission of 440 nm on a Synergy H4 plate reader (Biotek, Winooski, VT). Hoechst staining was used as a measure of total cell density/well ...
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bioRxiv - Plant Biology 2024Quote: ... and was adapted to 96-well microplates to be read on a multi-mode plate reader (Biotek SYNERGY-HTX, Winooski, VT, USA).53 Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... The intensity of the color is inversely proportional to the amount of ecdysteroid present as determined by spectrophotometry on a μQuant Microplate Spectrophotometer plate reader at 410 nm (BioTek Instruments, Vermont). The kit solutions were resuspended in UltraPure water (Cayman Chemical) ...
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bioRxiv - Plant Biology 2022Quote: ... SP Plant DNA Kit (Omega BioTek, Norcross, GA) (Pyne et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA Kit 1 (Omega BioTek, Norcross, GA). In conditions where we wanted to observe the effects in myotubes ...
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bioRxiv - Cancer Biology 2022Quote: ... was added to a final concentration of 0.1 mg/ml and incubated for 5 h prior to measuring the excitation/emission (544/590) (Biotek). Cells were reverse transfected (Lipofectamine 3000 ...
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bioRxiv - Immunology 2019Quote: ... Dialysis products were analyzed by 12% SDS–PAGE for purity and concentration was measured using Take-5 (BioTek Instruments). To test functionality of the produced rhTNFα ...
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bioRxiv - Cell Biology 2019Quote: ... a Lionheart FX automated microscope in combination with sirDNA77 staining was used to generate growth curves with a time resolution of 4 hours for a total time span of 136 hours (microscope maintained at 37°C, 5% CO2 using a 4× lens and a Sony CCD, 1.25 megapixel camera with 2 times binning; BioTek). Quantification of cell number was performed by Gen5 software (BioTek).
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bioRxiv - Biochemistry 2020Quote: ... The absorbance at 600 nm was monitored over time using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek) at 5 min time intervals for up to 12 hr with mixing ...
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bioRxiv - Cell Biology 2021Quote: ... The kinetics of the GTP hydrolysis was continuously monitored by the absorbance at 360 nm in Cytation 5 (BioTek) at 22 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... em 528/20) was collected every 5 minutes with a Synergy 2 multi-mode microplate reader (BioTek, Winooski, VT) for 6 hours at 37 °C.
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bioRxiv - Cell Biology 2021Quote: ... The absorbance at 600 nm was monitored over time using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek) at 5 min time intervals for up to 12 h with mixing and subtracted from a blank buffer with no turbidity.
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bioRxiv - Cancer Biology 2022Quote: ... HPTS and RuBPY fluorescence were monitored for 17 h using a Cytation 5 device (BioTek, Agilent, Winooski, VT, USA). Excitation was provided by a monochromator ...
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bioRxiv - Microbiology 2022Quote: ... The cell plate was infected with virus at approximately 0.5 TCID50/well and incubated at 37 °C in the Cytation 5 (BioTek) supplemented with 5% CO2 ...
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bioRxiv - Plant Biology 2019Quote: The growth rate of transformants was assessed using the Gen 5 data analysis software (BioTek Instruments, Inc. Winooski, VT) where duplicate cultures of replicate transformants were grown in SD GAL + RAF medium (SD medium ...
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bioRxiv - Synthetic Biology 2020Quote: ... the luminescence signals were quantified by using a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2022Quote: ... Luminescence was quantified with an integration time of 1s using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek).
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was recorded for 5 mins at 1 min intervals at 37°C (BioTek Neo; Ex. 494nm; Em. 520nm). After 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... RNA concentration was measured for both isolated RNA aliquots using a Cytation 5 Multi-Mode Reader (Agilent Biotek Instruments) with a Take3 Micro-Volume Plate at the wavelengths of 260 ...
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bioRxiv - Immunology 2023Quote: ... The luminescence from the wells was measured using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments, VT, USA). The luminescence from blank wells containing 120 µL DMEM with 10% fetal bovine serum and 50 µL CellTitre Glo reagent was recorded as baseline values ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The microplate was then incubated and allowed to equilibrate at 37°C in a microplate reader (BioTek Cytation 5) while kinetic fluorescence readings (ex./em. ...
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bioRxiv - Bioengineering 2024Quote: ... Primary Cells were stained with DAPI and then imaged with a high throughput imaging system (BioTek Cytation 5, Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then rinsed with PBS containing 1% FBS prior to imaging using a Cytation 5 instrument (Agilent BioTek). Mean fluorescence was calculated using Gen5 software (RRID:SCR_017317).
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Isolated gDNA was checked for quality and concentration by gel electrophoresis and by spectrophotometry using a Take3 Micro-Volume Plate (BioTek, Winooski, VT, USA). Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB) ...