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Citations for Biotek :
2351 - 2400 of 2549 citations for Progesterone ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... We performed PCA oxidation assays either by tracking the fluorescence of reduced PCA over time in a plate reader (BioTek Synergy 4 or HTX) housed in a Coy-brand anoxic chamber or by tracking the current generated by a culture when provided PCA and an electrode poised to continuously reduce any PCA that the cells oxidized ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Genomics 2020Quote: ... SQ RNA/DNA/Protein Extraction Kit (Omega Biotek Inc.). Briefly ...
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bioRxiv - Immunology 2024Quote: ... HP Total RNA Kit (Omega BioTek, CAT#R6812-02). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Microbiology 2024Quote: ... an E.Z.N.A soil DNA kit (Omega Biotek,GA, USA) was used to extract total genomic DNA ...
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bioRxiv - Physiology 2019Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Intra-assay CV was 4.44% and inter-plate CV was 5.94%.
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bioRxiv - Microbiology 2020Quote: ... Absorbance at 600 nm of all cultures was measured every 24 hours from 100 μL aliquots dispensed into 96-well flat-bottom plates using an Epoch microplate spectrophotometer (BioTek Instruments Inc. Winooski, VT, USA). Sterile 10% marine broth was used as blank to correct for background media absorbance ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by adding 50 μL 3M hydrochloric acid and absorbance at 492 nm was determined on a Synergy 4 plate reader (BioTek, Agilent Technologies inc., CA, USA) or similar ...
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bioRxiv - Microbiology 2021Quote: The lactobacilli growth rate analysis was performed at 30 and 37°C, under agitation (double orbital, fast mode) in Synergy H1 plate readers (Biotek Instruments, Inc. Winooski, VT, USA). OD was measured every 30 minutes for 24h ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... We calculated T concentration by comparing sample absorbance with the absorbance of the assay’s standard curve (Gen5 curve-fitting software, Biotek EPOCH plate reader, Winooski, VT, USA). Samples from 3 females and 2 males initially showed greater than 80% maximum binding ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 µL of culture supernatant was transferred to a new plate and analyzed for fluorescence intensity using BioTek Synergy H1 Multimode Reader (BioTek Instruments, Inc, Winooski, VT, USA). Percent Specific Lysis was expressed as a percent relative to maximum release control ...
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bioRxiv - Plant Biology 2023Quote: The aliquot of 100 µL taken from the lipophilic phase of the MTBE extraction was diluted 10-fold with methanol and the extracts were measured under an Epoch2 96-well plate reader (Biotek/Agilent; Santa Clara, CA, U.S.A.). Spectrum was measured at 470 nm ...
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bioRxiv - Synthetic Biology 2024Quote: ... Growth kinetics and related parameters during physiological characterization of engineered strains were derived from measurements of the optical density at 600 nm (OD600) with light path correction in a SynergyTM MX microtiter plate reader (BioTek Instruments Inc., Winooski, VT, USA). For whole-cell 2-pentanone production by engineered P ...
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bioRxiv - Synthetic Biology 2023Quote: ... and msfGFP fluorescence (lexcitation/lemission = 475 nm/510 nm) were measured along the experiment in a Synergy HI plate reader (BioTek Instruments, Inc., Winooski, VT, USA). Unless stated otherwise ...
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bioRxiv - Molecular Biology 2024Quote: ... upon which leaf discs were removed and absorbance at 538 nm was measured on a Biotek Synergy2 plate reader (BioTek Instruments Inc., Winooski, VT, USA). Absorbance readings were imported to GraphPad Prism 10 for macOS version 10.2.0 for plotting and analysis.
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bioRxiv - Microbiology 2024Quote: ... Biolog Phenotypic Microarray plates (Biolog Inc., Hayward, CA) were inoculated and incubated at 37 °C in a stationary incubator and A490 recorded (BioTek Synergy 2, Winooski, VT, USA). End point readings for carbon sources (PM1 and PM2 plates ...
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bioRxiv - Cancer Biology 2019Quote: ... Four random fields per coverslip were imaged at 20X on a Cytation 5 Imaging Multi-Mode Reader (BioTek Instrument, Winooski, VT) using DAPI and Texas red filters ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... and infected cells were maintained at 37°C for 14 to 16 h post infection before manual counting of eGFP+ and mNG+ cells or automated counting using a Cytation 5 cell imaging multi-mode reader (BioTek Instruments) and a CellInsight CX5 imager (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated with 2.5μM CellROX for 30min at 37°C after which culture media was replaced for imaging on a BioTek Cytation 5 imaging reader (BioTek, VT). Images were captured from 6 random areas of each well every 10min for 2h ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and GFP images were taken every 3 hours for 30 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). GFP quantification was performed by a Cell Profiler program (https://cellprofiler.org/) ...
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bioRxiv - Genetics 2022Quote: ... Brightfield and RFP-channel images were taken every 30 minutes for 12 hours using the Cytation 5 Cell Imaging Multi-Mode Reader (BioTek Instruments). Fluorescence quantifications and the counting of fraction of dead worms were performed by a Cell Profiler program ...
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bioRxiv - Cancer Biology 2022Quote: Cancer cell abundance was observed throughout treatment by imaging mono-cultures and co-culture spheroids using a Cytation 5 cell imaging multimode reader (BioTek Instruments) under dual Bright Field and YFP (ex 500 / em 542 for Venus florescence ...
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bioRxiv - Bioengineering 2019Quote: ... The natural luminescence of the seeds and the esterase activity was measured using a Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek Instruments ...
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bioRxiv - Synthetic Biology 2019Quote: ... The absorbance reading was done at 340 nm every 5 seconds for 3 minutes in a BioTek PowerWave XS microplate reader (Biotek, USA). For NADH at 340 nm 6.22 mM cm-1 extinction coefficient (εM ...
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bioRxiv - Biochemistry 2020Quote: ... The spectra were recorded using Synergy H1 Hybrid Multi-Mode Reader and Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek and were averaged by 3 scans.
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bioRxiv - Microbiology 2021Quote: ... coli cells suspensions were adjusted to an optical density at 600 nm (OD600; measured using a UV-visible spectrophotometer, Cytation 5, Biotek Inc.) measurement of 1 ...
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bioRxiv - Molecular Biology 2022Quote: ... and incubated at 95 °C for 5 min before the absorbance was measured at 540 nm using a microplate spectrophotometer (BioTek HT).
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bioRxiv - Physiology 2022Quote: ... three representative images of the samples were taken with a Cytation 5 cell imaging multi-mode reader with Gen5 software (Version 3.10; Biotek, Vermont, USA) and analyzed using ImageJ ...
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bioRxiv - Bioengineering 2023Quote: ... the fluorescence of each well was measured at 560/590 nm (excitation/emission) using a microplate reader Cytation 5 (BioTek Instruments). The number of viable cells per well was calculated against a standard curve prepared by plating various concentrations of isolated cells ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of Renilla luciferase activity by adding Stop & Glo® were performed according to the manufacturer instructions using a luminometer reading (Cytation 5 imaging multimode reader, Biotek).
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CRISPR-Cas9 Engineered Extracellular Vesicles for the Treatment of Dominant Progressive Hearing LossbioRxiv - Bioengineering 2023Quote: ... and the resulting emulsion was collected within an Eppendorf tube or the microplate analyzed under the inverted microscope Cytation 5 (BioTek, USA). The isolation of aqueous phase containing RNP-EV from the oil phase was performed under the centrifuge at 2000-3000xg for 5-10min at room temperature ...
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bioRxiv - Biochemistry 2024Quote: PLpro enzyme titration and initial velocity analyses were performed using a Cytation 5 multi-mode microplate reader (BIOTEK Instruments, Winooski, VT) as described previously 15 ...
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bioRxiv - Plant Biology 2019Quote: ... Plant RNA kit with DNA digestion on column (Biotek, China) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Plasmids were isolated using a DNA miniprep kit (Omega BioTek) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... or the E.Z.N.A.® HP Total RNA Kit (Omega BioTek). RNA was reverse transcribed to cDNA using the ABI Reverse Transcription Reagents system ...
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bioRxiv - Immunology 2023Quote: ... and E.Z.N.A.® HP Total RNA kit (Omega Biotek, U.S.). RNA samples were sequenced using the Illumina HiSeq platform to generate 50-bp paired-end reads ...
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bioRxiv - Developmental Biology 2022Quote: ... and using Total RNA Kit I (Omega Biotek, R6834-02) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Pure cultures of defined mutants were diluted to A600 0.003 and grown +/− antibiotic in 96-well microtiter format at 37°C with shaking in a plate reader (Tecan M200 Pro, Biotek Epoch 2, or Biotek Synergy H2M). Growth was monitored as change in A600 ...
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bioRxiv - Cell Biology 2023Quote: ... cell culture media from the bottom chamber of the Transwell system was collected (three technical replicates per Transwell) for spectrofluorometric analysis at 490 nm excitation/520 nm emission using a fluorescent plate reader (Biotek Synergy H4 or Biotek Synergy Neo2).
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bioRxiv - Cell Biology 2020Quote: ... values were normalized to the cell count using Hoechst dye in the final prot injection and transferred to the Cytation 5 (BioTek, Winooski, VT).
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bioRxiv - Cell Biology 2019Quote: Cells or spheroids were photographed with a microscope (Zeiss LSM 510) at indicated time points and videos were obtained with a high contrast instrument (Biotek, Cytation 5) for 72 hours.
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bioRxiv - Bioengineering 2020Quote: ... Wells were imaged at time of scratch and 24□h and 48 h later using Gen 5 software (BioTek Instruments, Winooski, VT) on a Cytation 5 plate reader (BioTek Instruments) ...
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bioRxiv - Cell Biology 2022Quote: ... COX activity was determined as the maximal rate of oxidation of fully reduced cytochrome c measured by the change in absorbance over time at 550 nm using an imaging multi-mode reader (BioTek, Cytation 5) at 30 °C ...
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bioRxiv - Microbiology 2020Quote: ... Samples were examined using an Olympus 1×81 spinning disk confocal microscope (Tokyo, Japan) or Cytation 5 Cell Imaging Multi-Mode Reader instrument (Biotek, Winooski, VT). Images were analyzed using Volocity or Gen5 software ...
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bioRxiv - Biochemistry 2021Quote: ... Daily cell number was determined by addition of 20 μl of AqueousOne Solution and measurement of absorbance at 490 nm using a Cytation 5 imaging system (BioTek, Winooski, VT). Fold change was calculated by dividing by absorbance measurements taken at day zero.
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bioRxiv - Microbiology 2021Quote: ... the polymerase activity was monitored by the detection of fluorescence reporter expression on a Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Sursee, Switzerland) equipped with a 4x air objective (numerical aperture (NA) ...
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bioRxiv - Microbiology 2019Quote: ... The cells were stained with DAPI and then visualized by the Cytation 5 Cell Imaging Multi-Mode Reader with objectives 40x PL FL and 20x PL FL (BioTek, Winooski, VT). Image acquisition was performed with BioTek Gen 5 Microplate Reader and Imager Software ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 50 ul of the reaction mixture was added to 200 ul of Stop solution and fluorescence was read using Cytation 5 (Biotek, Winooski, VA) at 360 nm (Excitation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The relative luciferase units (RLUs) in each well were detected using Cytation 5 Cell Imaging Multi-Mode Reader (BioTek, Winooski, VT, USA).
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bioRxiv - Microbiology 2020Quote: ... The reaction was stopped with 3M sulfuric acid 5-10 minutes after OPD addition and absorbance values were measured at a wavelength of 492nm on a spectrophotometer (Biotek Cytation5 system). Absorbance values were normalized by subtracting uncoated wells from antigen-coated wells at the corresponding serum dilution ...