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Citations for Biotek :
1901 - 1950 of 2032 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The growth behavior of the different mutants of AB5075-VUB was assessed in liquid culture using the Cytation 1 plate reader (BioTek, United States). Each well of a 96-well plate was filled with 200 µL of bacterial culture diluted to an initial OD600 of 0.1 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Microbiology 2023Quote: ... plates were briefly centrifuged at 500 xg and absorbance at 620 nm was then measured using a Cytation7 plate reader (Biotek, Winooski, VT). Raw absorbance values of the compounds were normalized to DMSO (0% inhibition ...
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bioRxiv - Neuroscience 2023Quote: ... and fluorescence emitted by Atto488 (λex = 485 nm, λem = 538 nm) was measured in a Synergy 4 plate reader (BioTek Instruments GmbH) at intervals of 10 seconds ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μg/ml) and grown for 20 hours before measuring optical density (OD) using a BioTek Synergy H1m plate reader (BioTek, Winooski, VT). We also conducted experiments with the E ...
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bioRxiv - Microbiology 2023Quote: ... and measurement of GFP (excitation 485 nm/ emission 528 nm) and dTomato (excitation 555 nm/ emission 585 nm) fluorescence using a BioTek Synergy plate reader (BioTek, Winooski, VT). Transformants with at least 3-fold increase in GFP expression were selected as candidate Zn reporter strains.
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bioRxiv - Biochemistry 2023Quote: ... Reactions were started by addition of 1 mM Mg-ATP and NADH absorbance was monitored at 340 nm in a plate reader (Biotek PowerWave HT) at 26°C ...
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bioRxiv - Cell Biology 2023Quote: ... the fluorescence of antibodies was measured at 633 nm excitation/665 nm emission using a plate reader (Biotek Synergy H4 or Biotek Synergy Neo2) in technical triplicate as we previously described41 ...
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bioRxiv - Cell Biology 2023Quote: ... for spectrofluorometric analysis at 490 nm excitation/520 nm emission using a fluorescent plate reader (Biotek Synergy H4 or Biotek Synergy Neo2).
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bioRxiv - Synthetic Biology 2023Quote: ... The plate was shaken at 300 rpm (orbital) for 3 min and BRET biosensor performance was recorded using the plate reader’s luminescence filter (BioTek Synergy H1, v2.04.11) with an integration time of 1 s ...
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bioRxiv - Microbiology 2022Quote: ... The absorbance of each well with reaction mixture and protein (but without formaldehyde) was measured at 340 nm with a BioTek Synergy H1 plate reader (BioTek, Winooski, VT) in a Corning CoStar 48 well plate (Corning ...
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bioRxiv - Biochemistry 2022Quote: Cy5.5-ALN was characterized in terms of absorbance in the NIR region using a plate reader (Cytation 5, BioTek, Winooski, VT, USA). Yield was calculated using ε675nm=211000 M/cm ...
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bioRxiv - Bioengineering 2022Quote: ... metachromatic changes of DMMB in presence of sGAG were determined using the Synergy HT multi-detection micro-plate reader (BioTek Instruments, Inc) at 530 and 590 nm ...
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bioRxiv - Bioengineering 2022Quote: ... were added and the hydroxyproline content quantified with a trans-4-Hydroxy-L-proline (Fluka analytical) standard using a Synergy HT multi-detection micro-plate reader at a wavelength of 570 nm (BioTek Instruments, Inc).
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bioRxiv - Bioengineering 2022Quote: ... were added and the hydroxyproline content quantified with a trans-4-Hydroxy-L-proline (Fluka analytical) standard using a Synergy HT multi-detection micro-plate reader at a wavelength of 570 nm (BioTek Instruments, Inc).
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bioRxiv - Immunology 2022Quote: ... on a plate reader with excitation at 585 nm and emission at 530 nm (Synergy H4 Hybrid Microplate Reader, BioTek Instruments, Inc.). Whole-intestine permeability at each time point in each mouse was assessed by subtracting the average relative fluorescence units of baseline plasma and triplicate wells of PBS (sample blank ...
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bioRxiv - Developmental Biology 2022Quote: ... Plates were maintained at 5% CO2 and 37°C during imaging using Cytation Gen5 Microplate Reader and Imager Software (BioTek, version 3.08.01). Protocols specific to Falcon 24 well companion plates (Corning ...
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bioRxiv - Microbiology 2022Quote: ... of the wild-type and mutant strains in 96 well microtiter plates (Surface Nunclon ™ Roskilde, Denmark) using a Sinergy H1 Hybrid Multi-Mode microplate reader (Biotek, USA). For this ...
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bioRxiv - Plant Biology 2022Quote: ... The reaction was followed by measuring the absorbance at 363 nm at 25°C for 15 min using a plate reader (BioTek Synergy 2). For NR activity measurements on material extracted from Arabidopsis ...
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bioRxiv - Cell Biology 2023Quote: ... was added according to the manufacturer’s instructions and the total luminescence was measured for 20 min using Synergy Neo plate reader (BioTek, New Castle, DE). Then agonists (10 μM carbachol (carbamoylcholine ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Hoechst staining a was read using an excitation of 392 nm and emission of 440 nm on a Synergy H4 plate reader (Biotek, Winooski, VT). Hoechst staining was used as a measure of total cell density/well ...
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bioRxiv - Plant Biology 2024Quote: ... and was adapted to 96-well microplates to be read on a multi-mode plate reader (Biotek SYNERGY-HTX, Winooski, VT, USA).53 Briefly ...
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bioRxiv - Developmental Biology 2024Quote: ... The intensity of the color is inversely proportional to the amount of ecdysteroid present as determined by spectrophotometry on a μQuant Microplate Spectrophotometer plate reader at 410 nm (BioTek Instruments, Vermont). The kit solutions were resuspended in UltraPure water (Cayman Chemical) ...
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bioRxiv - Microbiology 2024Quote: Lytic activity was assessed by inoculating logarithmic PA14 (107 CFU/ml) with the experimental samples and measuring turbidity with a plate reader (Synergy; BioTek, Winooski, VT) at OD600nm at 37°C with 5-second shaking every 20 min for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... with the each phage at a concentration of 108 PFU/ml and measuring turbidity using a plate reader (Synergy; BioTek, Winooski, VT) at OD600nm ...
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bioRxiv - Neuroscience 2024Quote: ... ELISA plates were read with a Synergy 2 Gen 5 microplate reader at 405 nm absorbance (BioTek Instruments Inc., Winooski, VT, USA).
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bioRxiv - Biochemistry 2024Quote: ... Cells were then diluted 100-fold in a 96-well plate either in the presence or absence of NMM and OD600 of the cells was measured every ten minutes over 24 hours with continuous shaking at 37 ºC in a plate reader (BioTek Synergy H1). Growth curves were done in triplicate ...
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bioRxiv - Biochemistry 2024Quote: ... and the pH-insensitive wavelengths (440ex/535em) every 15sec for 5 minutes at Medium PMT sensitivity on a plate reader (Biotek Cytation 5). After the initial kinetic pHi read ...
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bioRxiv - Bioengineering 2024Quote: ... and absorbance at 492 nm was monitored in one-minute kinetic interval cycles for 30 minutes using a plate reader (Biotek Cytation 5). The experiment was repeated for the samples after fabrication and biweekly upon storage in physiological conditions ...
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bioRxiv - Cell Biology 2024Quote: ... and the samples’ optical density (OD) was measured in a 96 wells plate at 540 nm using a spectrophotometer (Epoch 2, BioTek, Winooski, USA). ODs were corrected by the total number of cells counted (Countess 3 Cell Counter ...
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bioRxiv - Cell Biology 2020Quote: ... The purified extracts of CanlonicSF were excited at 485 ± 20 nm and the intensity of fluorescence emission of cpGFP was recorded at 530 ± 15 nm using the multiwell plate analyzer Synergy 2 (Biotek, Winooski, Vermont, USA). Excitation and emission spectra were obtained by collecting the emissions at 515 ± 15 nm using a multiwell plate reader EnVision (PerkinElmer ...
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bioRxiv - Microbiology 2020Quote: ... Isolated gDNA was checked for quality and concentration by gel electrophoresis and by spectrophotometry using a Take3 Micro-Volume Plate (BioTek, Winooski, VT, USA). Approximately 100 ng of gDNA was used as template for PCR using Q5 Hot Start DNA Polymerase (New England Biolabs (NEB) ...
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bioRxiv - Immunology 2022Quote: ... Reactions were stopped by the addition of 2M sulfuric acid and read using a BioTek Synergy 2 or Synergy H1 plate reader set at 450nm (BioTek, Winooski, VT, USA). Receiver operator curve (ROC ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were incubated ∼5 min at room temperature, and read by fluorescence (λex = 480 nm, λem = 520 nm) using a Synergy H1 plate reader (BioTek, Winooski, VT, USA).
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bioRxiv - Immunology 2021Quote: ... Total RNA was quantified using the Take3 microvolume plate accessory on a Cytation 5 Cell Imaging Multi-Mode Reader (Biotek, Winooski, United States), RNA purity was determined by analyzing A260/280 and A260/230 ratios ...
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Immunology 2021Quote: ... and absorbance was measured at 450 nm using a plate reader after stopping the reaction with 50 μl of 1 N HCl (Biotek Epok, Model # 3296573).
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bioRxiv - Microbiology 2021Quote: ... cells from overnight cultures were diluted to a fixed starting OD600nm of 0.01 in fresh LB medium supplemented with MgSO4 in 96-well cell culture plates (CellStar) and grown in a microplate reader Synergy2 (BioTek Instruments, Vt, USA) at maximum rpm at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Culture densities were monitored over time by measuring light scatter at 600 nm using either a Synergy HTX multi-mode plate reader (Biotek, Winooski, VT, USA) or an Ultraspec 10 density meter (Biochom ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ASChI hydrolysis was quantified by measuring changes in absorbance at 405 nm over 15 min using the Synergy H1 Hybrid Plate Reader with Gen5 2.0 software (BioTek Instruments, Winooski VT, USA). ChE activity was normalized against total protein concentration determined using the BCA assay according to the manufacturer’s directions (Pierce ...
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bioRxiv - Cancer Biology 2020Quote: ... Viability and cell proliferation were assessed by measuring the optical density at 570 nm in a plate reader (BioTek Eon,11-120-611).
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bioRxiv - Biochemistry 2020Quote: ... Elongation rates were measured using time-based scans on a plate reader (Biotek Synergy H4 Hybrid Multi-Mode Microplate Reader with Gen5 software, BioTek Instruments, Winooski, VT) at 25°C with excitation at 365 nm and emission detected at 407 nm.
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bioRxiv - Microbiology 2020Quote: ... were withdrawn at intervals 2.5 h during the bacterial growth for measurement of their optical density at 600 nm in 96-well plates (Synergy H1 Hybrid Multi-Mode Reader, Biotek, Winooski VT, USA). Cells in these aliquots were removed on centrifugation (10 min ...
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bioRxiv - Microbiology 2020Quote: At least three replicates per strain and growth condition were grown in 200 μl of LB alone or supplemented with canavanine in a 96-well plate at 30°C with 140 rpm shaking in a BioTek Eon Microplate Spectrophotometer (BioTek, Winooski, VT, USA). The A600 was measured at 10 minutes intervals ...
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Mitochondrial and metabolic remodeling in human skin fibroblasts in response to glucose availabilitybioRxiv - Cell Biology 2021Quote: ... and the luminescent signal was recorded using a BioTek® Cytation3™ UV–vis multi-well plate imaging reader (BioTek, Winooski, Vermont, USA).
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bioRxiv - Cell Biology 2022Quote: ... only 10 μL of MTS reagent was added to plate wells because there was not enough reagent to add 20 μL to all 96 wells.) End-point absorbance measurements were taken with a plate reader at 490 nm (BioTek Instruments, Agilent Technologies).
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bioRxiv - Cell Biology 2022Quote: ... 1 μl from overnight culture was transferred to the YPD and growth was monitored in 96-well plate using Epoch2 (BioTek, Winooski, VT, USA) kinetic growth analyzer by analyzing optical density of OD600.
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bioRxiv - Physiology 2021Quote: ... Lysates were incubated for 15 min at 37°C in presence of 10 μM SSP4 and fluorescence intensity (λex = 495 nm; λem = 520 nm) was measured in a Synergy Mx fluorescent plate reader (BioTek Instruments AG, Switzerland)
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bioRxiv - Molecular Biology 2021Quote: ... YP + α-KG (10g/l) or YPD (0.02% glucose) + α-KG and growth was monitored in 96 well plate using Epoch2 (BioTek, Winooski, VT, USA) kinetic growth analyzer by analyzing optical density of OD600 ...
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bioRxiv - Microbiology 2021Quote: ... These aerobic cultures were monitored periodically every 60 min for 37 °C growth at 600 nm in Synergy H1 plate reader (BioTek Instruments Inc, VT).