Labshake search
Citations for Biotek :
1601 - 1650 of 2032 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... or at an excitation wavelength of 472 nm and emission wavelength of 507 nm for 3WJdB-activated fluorescence on a plate reader (Synergy H1, BioTek). Fluorescence values for a fluorescein concentration in which a single replicate saturated the plate reader were excluded from analysis ...
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bioRxiv - Genetics 2021Quote: ... Nile Red fluorescence (excitation at 488 nm; emission at 565 nm) was measured in a multi-well plate reader (Synergy H1, Biotek), normalized to cell density (determined as absorbance at 750 nm ...
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bioRxiv - Immunology 2021Quote: ... was added to quench the reaction and the optical density at 490 nm of the culture plates was immediately measured using a Synergy LX multi-mode reader and Gen5 microplate reader and imager software (BioTek).
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bioRxiv - Cancer Biology 2020Quote: ... PpIX fluorescence in cell and tumor lysates was measured using a Synergy Mx Fluorescence plate reader (BioTek Instruments Inc. VT) with a 405 nm excitation/630 nm emission filter ...
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bioRxiv - Microbiology 2020Quote: ... Growth was measured in 96-well plates at 15 min intervals (180 µL culture overlaid with 70 µL mineral oil) with orbital shaking at 37 °C on a plate reader (Biotek). Representative data presented are the result of 6-12 plate replicates and were replicated independently at least 2 times ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored at an optical density at 600 nm (OD600) using either a spectrophotometer or a 96-well microtiter plate reader (Synergy H1; BioTek).
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bioRxiv - Microbiology 2021Quote: ... were transferred to white 96-well plates with clear bottom and the optical density (OD600nm) and the luminescence of the samples were measured using the plate reader Synergy H1 (BioTek). The integration time for luminescence detection was of 5 seconds ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Luminescence at 485 nm and fluorescent eYFP emission at 530 nm were measured for 1 second per well using a Cytation 5 plate Reader (BioTek). The ratio of eYFP/RLuc was calculated per well and data are presented as percent of dopamine response ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Increase in absorbance at 390 nm was recorded for 20 min at interval of 30 s with a kinetics mode program using a plate reader (Synergy, Biotek). The percent of inhibition was calculated by Vi/V0 ...
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bioRxiv - Cancer Biology 2020Quote: ... The amount of FITC-dextran in the serum was determined by measuring the fluorescence at an excitation wavelength of 485 nm and an emission wavelength of 525 nm using a plate reader (Synergy microplate reader, H1 Biotek).
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bioRxiv - Microbiology 2021Quote: ... the plate was incubated for an additional 1 h and then the fluorescence values at 565/600 nm excitation/emission were measured in plate reader (Synergy HTX, Biotek). The average value of the blanks was subtracted from each sample and the growth percentage was calculated relative to a growth control ...
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bioRxiv - Microbiology 2021Quote: ... were carefully taken and transferred to another polystyrene plate to measure hemoglobin release by its absorbance at 540 nm in an spectrophotometer (EPOCH Biotek). The absorbance of the hemoglobin from the erythrocytes incubated with Triton-X100 was taken as 100% hemolysis (Toro et al ...
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bioRxiv - Bioengineering 2021Quote: ... 100 µl of 1% Ribogreen reagent in TE buffer was added into each well, and the fluorescence (excitation wavelength 485nm, emission wavelength 528 nm) was measured by plate reader (Biotek).
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bioRxiv - Developmental Biology 2021Quote: ... the absorbance of the reaction mixture was measured at 520 nm with a microporous plate spectrophotometer (MQX200R2+Take3™, BioTek) according to the user’s manual ...
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bioRxiv - Microbiology 2021Quote: ... the reaction was stopped by adding 50 μl/well of 3M HCl and the plates read at an absorbance of 490 nm using a Synergy H1 hybrid multimode microplate reader (BioTek). The IC50 values were calculated by using GraphPad Prism 7.
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Immunology 2021Quote: ... after 1-5 minutes of adding TMB and absorbance at 450 nm was measured with a Synergy HTX plate reader (BioTek). Concentrations of antigens were determined using standard curves constructed with purified recombinant proteins and calculated with Gen5 3.02.2 (BioTek).
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bioRxiv - Immunology 2021Quote: ... was added to the cells and incubated in the dark for 15 min before reading on a Synergy H1 Hybrid Multi-Mode plate reader (Biotek). Measurements were done in duplicate ...
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bioRxiv - Microbiology 2021Quote: ... Sixteen fields were imaged per replicate at 40X using a Cytation 5 plate-reading microscope running Gen5 software (BioTek Instruments). To determine the number of parasites per vacuole ...
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bioRxiv - Immunology 2020Quote: ... After 5 to 10 min the reaction was stopped with 100 µl 1M sulfuric acid and the plates were read at 450 and 570 nm with the Cytation3 Imaging Reader (Biotek). The cut off value was defined as the average of all blank wells plus three times the standard deviation of the blank wells.
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bioRxiv - Pathology 2023Quote: ... The assay was performed at 37°C in 96-well format and the fluorescence recorded using Synergy H1 plate reader (BioTek). Mir05 buffer with 1 mM malate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RNA concentrations were determined at 260nm using a Take3 microvolume plate on a Synergy H1m microplate reader (Biotek, Winooski, Vermont). RNA samples (1 μg ...
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bioRxiv - Immunology 2022Quote: ... Reactions were quenched by addition of 1 N HCl and absorbance at 450 nm were analyzed using a plate reader (BioTek). ELISAs with gp120s and anti-idiotype monoclonal antibodies were performed as above except these proteins were immobilized directly onto high-binding 96-well assay plates (Costar ...
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bioRxiv - Microbiology 2023Quote: ... or reconstituted SIC cultures were then diluted 1:200 into four replicates in 96-well microplates in media as described above and grown in an Epoch2 or Synergy 2 plate reader (Biotek) for 48 h with constant shaking ...
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bioRxiv - Microbiology 2023Quote: ... Fluorescence of the Amcyan and Turborfp proteins produced by the spot colonies from each well was measured using the Synergy H1 plate reader (BioTek). We used the constitutive fluorescence of Amcyan to normalize TurboRFP production by reporter expression ...
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bioRxiv - Neuroscience 2022Quote: ... Cy3 dye signal in the respective input cell substrates and eluted products that result from the magnetic-based IP was analyzed in a CytationTM 3 automated fluorescence plate reader (BioTek).
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bioRxiv - Developmental Biology 2023Quote: Kidney cortex-isolated protein lysates were serial diluted and run-in triplicate on a 96-well plate reader at 590nm wavelength (Synergy HT, BioTek). A standard curve of bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2023Quote: ... 20 µl of each reaction were transferred into the wells of a black 96-well plate containing 180 µl ABT buffer and fluorescence values were determined using SynergyTM Mx microplate reader (BioTek) at 366 nm excitation and 445 nm emission wavelengths ...
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bioRxiv - Cancer Biology 2023Quote: The absorbance at 340 nm was measured every 10 or 12 seconds for 15 min by a Synergy HTX plate reader (BioTek). An NADH standard curve was used to determine the reaction rate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... were determined under burst and steady-state conditions using ceftazidime (Δξ = −9,000 M−1 cm−1) at 260 nm by measuring the initial enzymatic reaction rate in a Epoch plate-reader (Biotek). Burst phase rates were determined at 4°C and steady-state parameters were determined at 25°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Colonies were solubilized in 10% acetic acid to quantify clonogenic capacity and read for absorbance at 590nm in a plate reader (BioTek).
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bioRxiv - Molecular Biology 2023Quote: ... The culture was grown at 37 °C with continuous shaking and OD600 was measured for 16 h in Synergy Neo2 plate reader (BioTek). All readings were blank corrected ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of the fluorescent signal in the supernatant from each segment was determined utilizing a Synergy neo 2 multi-well fluorescence plate reader from BioTek/Agilent (excitation 545 nm and emission 590 nm) ...
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bioRxiv - Microbiology 2023Quote: ... Growth was monitored by measuring the OD at 600 nm every 20 min in a Synergy H1 plate reader (Biotek) with continuous double-orbital shaking (237 cpm ...
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bioRxiv - Microbiology 2023Quote: ... emission = 420 nm) was recorded for 60 min (reading was performed every minute) using a Synergy H1 plate reader (Biotek). On the fourteenth minute ...
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bioRxiv - Microbiology 2023Quote: ... The microtiter plates were incubated at 37°C and OD600 was measured every 10 minutes using Synergy H1 Hybrid Reader (BioTek) or Hidex Sense (Hidex Oy) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and cells were exposed to 200 µL BT ± CPC/Ag then immediately placed into the plate reader (Synergy 2, Biotek) for fluorescence measurement ...
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bioRxiv - Microbiology 2023Quote: ... The plate was incubated at 37 °C with continuous double orbital shaking for 16 hours in a Synergy Neo (Biotek) plate reader ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Fluorescence intensity was measured every 42 seconds for a duration of 1 hour at 37°C with a plate reader (Synergy 2, Biotek) (Temperature 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The Broccoli aptamer-BI fluorescence was measured upon 470 nm excitation and 510 nm emission in Synergy H1 plate reader (Biotek) for 2 to 8h at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... The microtiter plates were incubated at 37°C and OD600 was measured every 10 minutes using Synergy H1 Hybrid Reader (BioTek) or Hidex Sense (Hidex Oy) ...
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bioRxiv - Microbiology 2023Quote: ... bacterial cultures were incubated 24 h at 37°C in a plate reader (Synergy HTX Multi-Mode Reader; BioTek Instruments). During incubation ...
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bioRxiv - Systems Biology 2023Quote: ... 50 µL of samples were aliquoted in black 96-well (or alternatively 384-well) plates and measured with a Xenius spectrophotometer (or alternatively Synergy H1M microplate reader - BioTek) with an excitation wavelength of 280 nm and an emission wavelength of 360 nm.
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bioRxiv - Microbiology 2023Quote: ... was added to each well and then measured for luminescence using a Synergy 2 Gen 5 plate reader from BioTek.
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bioRxiv - Molecular Biology 2022Quote: ... Reactions were incubated in a fluorescence plate reader (most measurements used the Synergy™ H1 hybrid multi-mode reader, BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: ... for ∼30 min in darkness before optical density (OD) signals were measured at 650 nm using a Synergy HTX plate reader (Biotek). Endpoint dilution titers were calculated by determining the highest dilution that gave an average OD 650 reading greater than or equal to the cut-off OD value ...
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bioRxiv - Cancer Biology 2024Quote: ... Luminescence was measured after 15-min incubation at room temperature with luciferin detection reagent using a plate reader (Synergy H4 Microplate Reader, Biotek). The GSH/GSSG ratio was calculated using the following formula.
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bioRxiv - Biochemistry 2024Quote: ... Growth was monitored at 37 °C with orbital shaking and absorbance at 600 nm was measured every 10 minutes in a plate reader (Synergy H1 microplate reader, BioTek).
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bioRxiv - Microbiology 2023Quote: The rapidity by which tailocins killed bacteria was measured in 96-well plates (Nest) using a Epoch 2 microplate reader (BioTek). Twenty-five µl of a suspension containing tailocins (approximately 1011 particles mL-1 ...
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bioRxiv - Microbiology 2024Quote: ... was added to start the sialidase reaction and fluorescence 330/440 nm was measured while incubating at 37 °C with shaking in a plate reader (Biotek).