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Citations for Biotek :
1751 - 1800 of 2032 citations for glass bottom plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 50 % (0.25 mg/mL) were placed in a 96-well microtiter plate and incubated in a Synergy 2 Multi-Mode Reader (BioTek) at 37°C under continuous shaking during 40 min ...
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bioRxiv - Bioengineering 2024Quote: ... The plate was then quickly spun down at 3000 rpm and scanned from 510 to 580 nm nm in increments of 2 nm on Synergy Neo2 Multi-Mode Plate Reader (BioTek). Values reported are averages of duplicate wells and are sample red shift wavelengths at maximum absorbance subtracting the blank reference (PBS only) ...
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bioRxiv - Systems Biology 2024Quote: ... of yeast cell suspensions were dispensed into the 384-well plates containing compounds or DMSO with a EL406 automated dispenser (Biotek). Plates were covered and incubated at optimized temperature for 24-48 hours ...
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bioRxiv - Systems Biology 2024Quote: ... Cells from overnight cultures were resuspended in appropriate media to an optimized OD6oo and 20 µL of yeast cell suspensions were dispensed into the 384-well plate containing compounds or DMSO with a EL406 automated dispenser (Biotek). Plates were covered and incubated at appropriate temperature for 24-48 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and three N128-138 peptides of the SARS-CoV-2 N-protein with the GFP-tagged G3BP1NTF-2 protein was determined using a fluorescence-based PPIs assay.92–94 The assay was performed using a Synergy HTX multimode plate reader (Agilent BioTek) equipped with Gen5 software ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Stationary-phase Ancestor and EPD cultures were all diluted to the same starting optical density (as measured by the 96-well plate reader: BioTek EPOCH2T), so that all cultures in column 12 of each plate have the same starting optical density ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were grown by shaking at 225 rpm at 37°C for 48 hours in a shaking incubator or in a plate reader shaking orbitally at 282 cpm at 37°C for 48 hours (BioTek Epoch2).
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bioRxiv - Neuroscience 2021Quote: ... and optical absorbance at 450 nm was measured with a Synergy™ HT Multi-Mode Plate Reader (Biotek Instruments, Winooski, VT). The concentrations of adropin in plasma samples were determined from a four-parameter logistic curve fitted for the standard peptide absorbance values.
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bioRxiv - Molecular Biology 2021Quote: ... and wells were washed three times with 300 μl of diluted washing solution (soak time > 5 seconds) using an automated plate washer (BioTek 50TS). After washing ...
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bioRxiv - Microbiology 2022Quote: ... Plates were arranged in technical triplicate for each strain and condition and incubated in a Synergy Neo2 Microplate Reader (BioTek, USA) according to the following protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... and incubated at 37 °C (with a 1 °C gradient to minimize condensation) while shaking at 807 double-orbital cycles per minute in a plate reader (BioTek, Neo2SM). Optical density at 600 nm (OD600 ...
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bioRxiv - Microbiology 2021Quote: ... The grown cell culture in a microtitre plate was excited at 405 nm and the emitted fluorescence was measured at 467 nm in a multi-mode plate reader (BioTek, USA) [20].
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bioRxiv - Bioengineering 2021Quote: ... and we measured the excitation at 485 nm and the absorption at 528 nm with black 96-well plates with multi-detection microplate reader (BioTek, USA). The DNA probes used in the EMSA were amplified by PCR with CreF1/CreR using the B ...
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Salinomycin inhibits epigenetic modulator EZH2 to enhance Death Receptors in Colon Cancer Stem CellsbioRxiv - Cancer Biology 2020Quote: ... Bound dye was dissolved in 10mM Tris base and absorbance was measured at 510 nm on a plate reader (Epoch Microplate Reader, Biotek, USA). The cytotoxic effects of compounds were calculated as % inhibition in cell growth as per the formula [100-(Absorbance of compound treated cells/ Absorbance of untreated cells)] X 100.
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bioRxiv - Neuroscience 2020Quote: ... Fluorescence intensities of the acidic solutions were measured at an excitation/emission of 443/505 nm on A Cytation 5 multimode plate reader (BioTek, Inc). Next ...
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bioRxiv - Biochemistry 2021Quote: ... The released pNP (extinction coefficient ε=16,000 M-1cm-1) was measured at 405 nm using Epoch 2 plate reader (Biotek, US) set to 37°C and subtracting the blank reading ...
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bioRxiv - Systems Biology 2021Quote: Vmax assays for comparison of yeast and human cell extracts were carried out with freshly prepared extracts via NAD(P)H-linked assays at 37 °C in a Synergy H4 plate reader (BioTek™). The reported Vmax values represent total capacity of all isoenzymes in the cell at saturating concentrations of all substrates and expressed per extracted cell protein ...
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bioRxiv - Systems Biology 2020Quote: ... according to manufacturer’s instructions and nitrite concertation was calculated based on standard curve measuring optical density at 540 nm on a plate reader (BioTek Synergy2). Corticosterone was measured colorimetrically in plasma using DetectX Corticosterone Enzyme Immunoassay Kit K014-H1 (Arbor Assays ...
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bioRxiv - Plant Biology 2021Quote: ... and 663 nm by calibrating to zero with pure DMSO and was measured with a spectrophotometer plate reader (Synergy HT Multi-Detection Microplate Reader; BioTek Instruments). Measurements were done in 2 biological replicates ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots of 3 µL were added to triplicate wells containing 100 µL of BHIS in the outer wells of a 96-well plate and growth was recorded over time at OD600 using an Eon high-performance microplate spectrophotometer (BioTek Instruments) with constant shaking between readings ...
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bioRxiv - Microbiology 2021Quote: ... and then optical density and fluorescence were monitored for 24-72 hours in an Biotek Synergy H1M plate reader (BioTek, USA) at 30 °C with fast continuous shaking ...
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bioRxiv - Systems Biology 2021Quote: ... The initial rate of formation of NAD(P)H was followed by spectrophotometry at 340 nm in a Synergy HI plate reader (BioTek Instruments).
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bioRxiv - Molecular Biology 2020Quote: ... The resultant colour was measured at 410 nm using a plate reader spectrophotometer (Cytation 3 Imaging Reader, BioTek, Winooski, VT, USA). A standard curve of triglycerides ranging from 0 to 60 μg was run independently and in parallel with the experimental samples ...
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bioRxiv - Cancer Biology 2020Quote: Normal prostate epithelial cells and prostate cancer cells were seeded at optimal density in 384-well plates using an automated dispensing system (BioTek EL406). EZH2 inhibitors (GSK126 or EPZ-6438 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were trypsinized and added to the drug plates (2000 cells/well) with a Multi-Flo liquid dispenser (BioTek, Winooski, VT), then placed in a humidified incubator for 72 hours at 37°C supplemented with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 170 μL of glacial acetic acid were added to each well and the plate was read in a Synergy microplate reader (BioTek, USA) at Abs = 570 nm ...
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bioRxiv - Cell Biology 2020Quote: ... was measured at 37°C from 1 to 10 min using a fluorescence microtiter plate reader (Synergy HTX Multi-Mode Reader, BioTek, USA). eNOS activity was expressed as the VEGFA-dependent increase in fluorescence per μg of cellular protein ...
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bioRxiv - Bioengineering 2022Quote: Luminescence was measured on a plate reader (BioTek Synergy™ HTX for collecting data presented in Figures 1, 3, 5, S5, S8, S12, S14; BioTek Synergy™ Neo2 for Figures 2C-D ...
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bioRxiv - Evolutionary Biology 2022Quote: ... the initial 8 replicates for each concentration of the antibiotic exposure were incubated at 37°C and shaking in a 96-well-plate and the optical density (OD600) was measured every 20 minutes for 24 h in a plate reader Synergy H1 (Biotek, Germany). Fig ...
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bioRxiv - Plant Biology 2022Quote: ... Resulting pellets were dissolved in 1 ml 0.2 N NaOH and the corresponding optical density (OD) at 630 nm was measured via a plate reader (Synergy™ HTX Multi-Mode Microplate Reader; BioTek). Protein concentration (C ...
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bioRxiv - Neuroscience 2022Quote: ... chromogen substrate solutions were added and plate reading conducted in a microplate photometer (SyneryTM Neo2 Multi-mode Microplate Reader; BioTek Instruments) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The optical density of plates was evaluated at 450 nm using a microplate reader (BioTek Synergy H1 Hybrid Multi-Mode Reader). EC50 values were estimated using the ATT BioQuest® - EC50 calculator.
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bioRxiv - Plant Biology 2021Quote: ... Absorbance of 50 μl aliquots was determined at 648 and 665 nm using a Cytation 5 plate reader (BioTek, Winooski, VT) and content of chlorophyll a and b per μg wet weight determined using the equations in Barnes et al ...
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bioRxiv - Microbiology 2020Quote: ... Bacterial growth was monitored by measuring optical density at 600 nm every 15 minutes in a 96-well plate (Biotek Eon). Mid-log phase cells were harvested by centrifugation (4,000 x g ...
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bioRxiv - Systems Biology 2022Quote: ... The prepared microplates (with bacteria and gemcitabine dilutions) were incubated at 37°C and 360 rpm double orbital shaking in an automated plate reader (BioTek Eon) and absorbance (600 nm ...
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bioRxiv - Cell Biology 2020Quote: ... the samples were incubated at room temperature for 10 minutes and read using the Cytation 5 96 well plate reader (BioTek, Vermont) and Gen5 software (version 2.07.17 ...
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bioRxiv - Genetics 2020Quote: Yeast fluorescence was measured in 24-hour time courses during overnight growth in a BioTek Synergy H1 plate-reader (BioTek Instruments). Fresh cells from agar plates were inoculated in 100 μl of minimal YNB media containing any complements necessary for growth of auxotrophic strains ...
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bioRxiv - Pathology 2021Quote: ... Crystal violet was solubilized with 200 ml of methanol and measured with a plate reader (OD 540; BioTek, Winooski, VT, USA). Proliferation was assessed by comparing the change in OD 540 at 24 ...
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bioRxiv - Biochemistry 2020Quote: ... Transfer 100 µL of the solubilized crystal violet to another plate and quantify the absorbance at 550 nm using Cytation™ 5 Cell Imaging Multi-Mode Reader from BioTek.
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bioRxiv - Molecular Biology 2021Quote: ... the samples’ absorbance was measured at 494 nm using a 1 cm pathlength cuvette and a plate reader spectrophotometer (PowerWave XS, BioTek Instruments). No free fluorophore was detected in the cassettes that had only AF488 (without collagen) ...
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bioRxiv - Physiology 2021Quote: ... Fluorescence intensity (λex = 495 nm; λem = 520 nm) was measured continuously in a Synergy Mx fluorescent plate reader (Biotek AG, Switzerland) at 37 °C before and after addition of various compounds ...
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bioRxiv - Systems Biology 2021Quote: ... the 4% formaldehyde solution was aspirated and the cells were washed three times with 60 μL PBS using an automated plate washer (BioTek EL406). Cells to be stained for phospho-STAT1 were further permeabilized with ice-cold 100% methanol (Sigma Aldritch #34860 ...
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bioRxiv - Neuroscience 2021Quote: ... Purified total RNA was quantified on a Synergy 2 Multimode Microplate Reader using a Take3 micro-volume plate (BioTek, Winooski, VT). For cDNA synthesis ...
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bioRxiv - Cell Biology 2021Quote: ... The plate was incubated for 5 min at 37 °C and the change in absorbance was monitored continuously over 20 min using ELISA plate reader (Biotek, USA) at a wavelength of 340 nm.
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bioRxiv - Microbiology 2021Quote: ... The depth of color (absorbance) was measured at 405 nm using an automated ELISA plate reader (model EL311SX; Biotek, Winooski, VT). The final Ig isotype concentration in the antibody sample was calculated using an appropriate standard curve ...
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bioRxiv - Immunology 2020Quote: ... Samples were incubated for 1 hour at 37°C with 5% CO2 and OD440nm & OD650nm were then measured using a 96-well plate spectrophotometer (Molecular Devices or BioTek). OD650nm was subtracted from OD440nm then percent viability was calculated relative to the no treatment condition.
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bioRxiv - Bioengineering 2021Quote: ... Dextran fluorescence in the basolateral and apical chambers was measured using the Synergy H4 plate reader (Biotek Instruments Inc., Winooski, VT) and was converted to a dextran concentration using dextran-fluorescein standard curves ...
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bioRxiv - Immunology 2021Quote: ... either 25 μL of Nano-glo substrate or CTZ substrate was added to each well and the Biotek Synergy Mx plate reader (Biotek, VT) was used to measure luminescence ...
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bioRxiv - Bioengineering 2021Quote: ... the subsequent outflows from apical and basal channels were collected over the next 24 h and used for barrier function analysis by quantifying Cascade blue fluorescence intensities using a multimode plate reader (BioTek NEO). The apparent permeability was calculated using the following formula:
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bioRxiv - Microbiology 2020Quote: ... Plates were washed 5 times with S Basal then liquid was aspirated using an EL 406 Plate washer / Liquid dispenser (BioTek, VT). 50 μL of 0.01% tween was added to each well to limit worms adhering to pipette tips ...