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Citations for R&D Systems :
4351 - 4400 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the astrocyte-enriched cell suspension was incubated for 20 minutes at room temperature on a dish coated with anti-HepaCAM antibody (R&D Systems MAB4108) to bind astrocytes ...
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bioRxiv - Microbiology 2021Quote: ... One million cells were re-suspend in 100 μl PBS and either immediately incubated with 0.25 μg anti-ACE2 antibody (R&D systems, Cat.# AF933) or first incubated at 37 °C for 6 hours with shaking to allow recovery of cell surface proteins after trypsinization ...
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bioRxiv - Synthetic Biology 2021Quote: ... Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were removed from adherent cultures using AssayComplete cell dissociation reagent (Eurofins #92-0009) and incubated with a polyclonal anti-ALK2 antibody (R&D Systems #AF637) or a polyclonal anti-ALK3 antibody (Sino Biological #50078-RP02) ...
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bioRxiv - Genomics 2021Quote: ... a total of ∼200 million cells were harvested and stained for surface ACE2 abundance as previously described with ACE2 antibody (R&D Systems #MAB9332) at 1:50 dilution in FACS buffer (PBS supplemented with 2% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were left for 48h to allow expression of LepRb-HA protein before treatment with recombinant murine leptin (R&D System #498-OB) at 0-10 ng/L for 30 minutes before cell lysis and western blotting for phosphoSTAT3 (Y705) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:500, rabbit anti-PDGFRα (3164 Cell Signalling Technologies, Danvers, Massachusetts) 1:500 and goat anti-Sox10 (AF2864 R&D Systems, Minneapolis, Minnesota) 1:50 ...
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bioRxiv - Genetics 2023Quote: ... A total of 1 million cells per well were plated in 12-well Cultrex-coated (R&D Systems, catalog no. 3434-010-02) tissue culture plates (Corning Costar ...
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bioRxiv - Molecular Biology 2023Quote: ... For FGF2 treatment sub-confluent MIO-M1 cell cultures were serum starved overnight prior to treatment with 1 ng/ml FGF2 (R&D Systems, USA) and 10 μg/ml heparin for indicated times ...
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bioRxiv - Cancer Biology 2024Quote: ... A time course was performed on cells treated with 300 ng/mL of recombinant mouse VEGF-B186 (R&D Systems #767-VE-010) for 0 ...
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bioRxiv - Immunology 2023Quote: ... T cells were analyzed using ELISpot according to the manufacturer’s protocol ELISpot Rat interferon-gamma (IFNγ) (Cat: EL585 by R&D Systems, United States).
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bioRxiv - Cancer Biology 2023Quote: ... HUES8 cells were treated with 3μM CHIR99021 (CHIR, Stem-RD, Hoover, AL) and 100 ng/mL Activin A (AA, R&D systems, Minneapolis, MN) in RPMI media (Cellgro ...
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bioRxiv - Cancer Biology 2023Quote: AREG-neutralising experiments were performed by pre-incubating freshly prepared EVs (at 4 x 103 EVs per recipient cell) with either 4 µg/ml of goat anti-AREG (R&D Systems #AF262) or goat anti-IgG (R&D Systems #AB-1080-C ...
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bioRxiv - Immunology 2023Quote: The two cytokines IFNγ and TNFα were detected from cell culture supernatants of the degranulation assays utilizing the respective R&D DuoSet (R&D Systems, USA) ELISAs per manufacturers’ recommendation.
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bioRxiv - Developmental Biology 2023Quote: ... Subsequently cell clusters were differentiated into β-like cells by exposure to the appropriate media as previously published.29 All recombinant proteins were purchased from R&D System unless otherwise stated (see Supplementary Table 3).
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bioRxiv - Cancer Biology 2024Quote: ... mice (6-8 weeks old) were injected with 1x106 cells suspended in PBS and Cultrex™ basement membrane extracts (BME) (Bio-techne/R&D Systems) into the right flank ...
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bioRxiv - Immunology 2024Quote: ... cells were stained for 40 min at 4°C with fluorophore-conjugated antibodies PE-anti-Glut1 (clone 202915 R&D Systems, Minnesota, USA) and in parallel ...
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bioRxiv - Neuroscience 2020Quote: ... mouse PFC fractions were dissociated with a tissue homogenizer in 100 μl of 0.5 × cell lysis buffer 2 diluted in PBS (R&D Systems., Minnesota, United State). Tissues were lysed at room temperature for 30 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Mullins’ laboratory (Voigt et al., 2019) at the University of Iowa and grown in endothelial cell growth medium (R&D Systems, Minneapolis, MN, USA). iCEC2 cells contain a temperature-sensitive hypomorphic T antigen and actively proliferate at 33°C ...
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bioRxiv - Pathology 2022Quote: ... BMMs were plated into 96-well or 24-well plates (3 × 104 cells per milliliter) in complete α-MEM culture media supplemented with 50 ng/mL RANKL (R&D Systems, USA) and 25 ng/mL M-CSF (PeproTech ...
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bioRxiv - Cancer Biology 2020Quote: ... were used for this study.1.8×106 22Rv1 cells stably transduced with doxycycline-inducible shScramble or shTMEFF2-9 shRNAs and mixed with Basement membrane extract (R&D systems, Minneapolis, MN) at a 1:1 ratio ...
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bioRxiv - Neuroscience 2021Quote: Differentiated BMDM were plated in 96-well plate at density of 30,000 cells/well either in the presence of 40ng/ml M-CSF (R&D systems, 416-ML-050) or without M-CSF ...
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bioRxiv - Microbiology 2021Quote: ... Domes were dislodged with a cell scraper in 1 mL of Cultrex Organoid Harvesting solution (Bio-techne, R&D Systems brand, 3700-100-01) and incubated for 1 hr at 4°C on a shaker at 250 rpm ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.2 x 106 WT or Lyn KO Maver-1 cells or 1 x 106 primary CLL cells were seeded into the transwell upper inserts while media were supplemented with chemokine CCL19 (#361-MI-025, R&D Systems, Minneapolis, MN) at 200 ng/mL or 0.1% BSA in PBS (control ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in appropriate cell culture medium as directed by supplier with 10% heat-inactivated fetal bovine serum (FBS) (R&D Systems; Minneapolis, MN) and 1% Penicillin-Streptomycin (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... Confirmation of ETB gene expression was confirmed in cell populations by surface staining with antibodies (anti-HA tag AlexaFluor 488 conjugated mouse IgG, R&D Systems, cat # IC6875G) in combination with flow cytometry ...
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bioRxiv - Cell Biology 2024Quote: ... Seven different ligand conditions were tested at concentrations previously determined to elicit maximal cell responses49 (EGF 10 ng/ml (R&D Systems #236-EG), OSM 10 ng/ml (R&D Systems #8475-OM) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated in the presence of 50 ng/mL of GM-CSF (02532, Stem Cells, Vancouver, Canada) and 50 ng/mL of M-CSF (216-MC, R&D Systems, Minneapolis, USA) at 37°C and 5% CO2 for differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... non-adherent hematopoietic progenitors were pooled and plated at 7x104 cell/well (96-well) with complete media supplemented with 30 ng/mL M-CSF (R&D Systems, 416-ML). At 90% confluence ...
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bioRxiv - Genetics 2023Quote: ... the cells were first seeded in a 96-well plate at a density of 50,000 cells per well in RDM containing 10% FBS and 10 µM ROCK inhibitor (R&D Systems; Cat# Y-27632). On Day 2 ...
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bioRxiv - Bioengineering 2024Quote: ... Stem cells underwent directed differentiation into definitive endoderm over a 3-day treatment using Activin A (100ng/mL, R&D Systems Cat#338-AC) added to RPMI base media ...
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bioRxiv - Physiology 2019Quote: ... Cells were washed with BEC medium containing 10% FBS and were re-suspended in Flow Cytometry Staining Buffer (1X) (R&D Systems, Minneapolis, MN, USA) supplemented with 1% FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... CHLA9 and CHLA10 cells were cultured in IMDM +L-glutamine media supplemented with 20% FBS and 1% insulin-selenium-transferrin (ITS, R&D Systems, cat no: AR013). Cell lines were maintained at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... For differentiation assays cells were cultured in differentiation media (DMEM + 1% FBS + 1% P/S) with or without recombinant TGFβ (R&D systems, 10 ng/mL) and analyzed 72 hours later by immunofluorescent staining described below ...
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bioRxiv - Physiology 2021Quote: ... Cells were then incubated overnight at 4°C with goat anti-mouse platelet endothelial cell adhesion molecule (CD31/PECAM-1) primary antibody (R&D Systems cat. No. AF3628) diluted in antibody buffer (1:1000 ...
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bioRxiv - Immunology 2020Quote: ... Cells were isolated by flow cytometric cell sorting on a BD FACSMelody and cultured in StemPro-34 supplemented with either M-CSF or VEGF (R&D Systems, cat #293-VE).
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bioRxiv - Bioengineering 2022Quote: Transduced/Transfected and sorted hGICs harbouring either Lentivirus-MGT#1 or PiggyBac-MGT#1/4 were seeded as single cells into 6-well plates in RHB-A medium supplemented with 10ng/ml TNFa (R&D Systems, 210-TA-020) or without TNFa as control ...
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bioRxiv - Cell Biology 2022Quote: ... BMMs were then seeded at 1 × 106 cells/well in 24-well plates and stimulated with 30 ng/mL RANKL and 30 ng/mL M-CSF (R&D Systems, Minneapolis, MN, USA) for 5 days to generate mature OCs ...
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bioRxiv - Genomics 2024Quote: ... The human RPTEC with early passages were seeded at 8 × 105 cells on 10 cm culture dish at the day before the assay and treated with or without TNFα (R&D systems, 100 ng/ml) at 3 h before the fixation ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were sectioned at 3 µm and stained using the Bond-Max device (Leica) (CD4: Cell Signaling Technology, 25229S; CD8a: Synaptic Systems, 361003; FoxP3: R&D Systems, MAB8214 ...
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bioRxiv - Immunology 2023Quote: ... the cells were plated at 0.2M cells/ml in a 6-well plate and stimulated with IFNβ (500 IU/ml, R&D systems cat. 8499-IF-010). At 24h ...
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bioRxiv - Cell Biology 2022Quote: Proteasome activity was assessed similarly by incubating cells with genetic (7 days) or chemical (1 hour) perturbations with Me4BoVS (R&D system; I-190-050). After incubation at 37ºC for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: FBXW11 WT and FBXW11 KO cell lines (A549 and Ishikawa) were treated with 20 ng/ml rhIL-17A (R&D Systems, #7955-IL-025/CF), rhIL-17B (R&D Systems ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were then incubated with horseradish peroxidase–conjugated secondary antibodies (1:4000: Cell Signaling Technology; 1:2,000: R&D Systems, Bio-Techne; Santa Cruz Biotechnology) for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: Preparation of RSPO1 conditioned medium was performed from HA-R-Spondin1-Fc 293T Cells according to a distributor’s protocol (R&D systems Catalog# 3710-001-K). The RSPO-1 producing cells were a kind gift from Prof ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft studies were performed by subcutaneous bilateral implantation of 5E6 tumor cells/injection suspended 100 µl of 1: 1 RPMI / BME type 3 (R&D Systems #36-320-1002P). 4 to 6-week-old NOD-scid IL2Rgnull (NSG ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice with PBS and further cultured in N2B27 medium supplemented with 20 ng/ml bFGF (R&D Systems, cat. 233-FB-025/CF) and 20 ng/ml Activin A (PeproTech ...
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bioRxiv - Cancer Biology 2019Quote: ... 2×105 cells were cultured in CH12 medium supplemented with a mixture of IL4 (10 ng/mL, R&D Systems #404-ML-050, Minneapolis, MN, USA), TGFβ (1 ng/mL ...
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bioRxiv - Bioengineering 2019Quote: ... cells were plated at 1 million cells per well in 2mL mESC media (without LIF) + 25 μg/L Activin A (R&D Systems 338-AC-050/CF), 5 μg/L BMP4 (PeptroTech 315-27) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were starved in 0.1% FBS for 24 hours prior to addition of EGF (R&D systems #236-EG, diluted in 1 mg/ml BSA/PBS) to 25 ng/μl or carrier alone for 5 min at 37ºC ...