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Citations for R&D Systems :
4301 - 4350 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Cells were then treated with 200ng/mL of recombinant rat NogoA (1026-1090aa) and Fc Chimera protein (Nogo-66; R&D Systems), or with human NogoA (566-748aa ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were immunostained with rat anti-GFP (1:2000, GF090R; Nacalai Tesque) and goat anti-TrkB (1:250, AF1494; R&D Systems). Cells were extensively washed and incubated with appropriate secondary antibodies at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Single cell suspensions of wild type and Pds5a KO mESCs were permeabilized and fixed using the fixation/permabilization buffer (R&D systems), washed twice with 1x PBS and stained using the H/MM pluripotent Stem Cell Multi-Color Flow Cytometry kit (R&D Systems ...
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bioRxiv - Cell Biology 2020Quote: ... while 29-13 cells were passaged in Beck’s Medium supplemented with 15% doxycycline-free fetal calf serum (R&D Systems—Minneapolis, MN), 50 μg mL-1 hygromycin (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... microglial cells were preincubated with 20µg/ml of anti-C3aR antibody (Catalog # MAB10417) or control IgG (R&D Systems, Inc. Minneapolis, MN) suspended in DMEM/F12 supplemented with 5% heat-inactivated FBS ...
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bioRxiv - Immunology 2022Quote: ... The cells were again divided and incubated in the presence or absence of 50 ng/ml TGFβ (R&D Systems, Minneapolis, MN). Cells were harvested at 30 minutes and 24 hours post addition of TGFβ and analyzed by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... six- to eight-week old NSG mice were subcutaneously injected into the flank with 2×106 wildtype or pre-transduced A-673 or RD-ES EwS cells in Cultrex Basement Membrane Extract (R&D Systems) to enhance tumor formation ...
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bioRxiv - Cancer Biology 2021Quote: ... Intracellular granzyme B and INFγ staining was performed in cells that had fixed and permeabilized in Flow Cytometry Fixation and Permeabilization Buffers (Catalog numbers FC004, FC005, R&D systems) following manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Intestinal tissues from mice treated with PBS or spore for 7 d and 45 d were lysed with cell lysis buffer (R&D Systems) supplemented with 1% 0.2 mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Biophysics 2019Quote: ... and Fc-ICAM-1 or Fc-VCAM-1 (Intercellular Adhesion Molecule 1; Vascular Cell Adhesion Molecule 1, R&D system, Minneapolis, MN) solution at 10 μg/mL overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 5 × 105 cells in 100 μl adhesion buffer were added to the upper chamber and either 500 ng/ml CCL2 (R&D Systems) or 10 nM C5a (R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... live cultures were incubated for 1h in a cell culture incubator with culture medium containing 1:500 anti-PODXL antibody (MAB1556, R&D Systems). The cells and BME drops were subsequently fixed with 4% PFA ...
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bioRxiv - Developmental Biology 2019Quote: ... or β-CATENIN cells were grown in Basal medium in 60 mm dishes and treated with either 50 ng/ml ActA (R&D systems) or with WNT3A/L-cell conditioned medium (L-CM)11 for 6h ...
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bioRxiv - Bioengineering 2019Quote: ... To induce differentiation FGF2 was withdrawn from the cell culture media and then replaced with bone morphogenic protein (BMP4; R&D Systems) or and Wnt3a (R&D Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated for 24 hours either with vehicle or with 10nM GnRH and 1nM activin A (R&D Systems, Minneapolis, MN) in serum-free DMEM with 0.1% BSA.
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bioRxiv - Developmental Biology 2021Quote: ... cells were exposed to 1 µM EdU along with or withpout 2-100 ng/ml recombinant VEGFA (R&D Systems, 493-MV) for 8 hours before being fixed and stained by the Click-iT EdU Alexa Fluor 488 Imaging Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with either M1-activation medium [RPMI160 medium with 20 ng/ml IFNγ (485-MI-100; R&D systems) and 10 ng/ml LPS (L2630 ...
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bioRxiv - Developmental Biology 2020Quote: ... a brief wash with DMEM/F12 is followed with Day0 medium 30ng/ml Activin A (Cell Guidance Systems) + 40ng/ml BMP4 (R&D Systems) + 6uM CHIR99021 (Tocris ...
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bioRxiv - Cancer Biology 2022Quote: ... The murine MLL-AF9 AML cells (30) were cultured in the same condition with 10 ng/mL rmIL-3 (R&D systems). Culturing of normal murine HSPCs and human CD34+ UCBs cells has been described previously (31).
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bioRxiv - Bioengineering 2022Quote: ... EC population purity was evaluated on day 12 via live-cell flow cytometry using an anti-CD31 – Alex 488 conjugated antibody (1:100, R&D Systems). Only EC populations with ≥ 90% CD31-positivity were used for this study.
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bioRxiv - Cancer Biology 2022Quote: ... 22Rv1 cells expressing dox-inducible shNT or shUGUUUGC shRNAs were mixed with 50% basement membrane extract (R&D Systems, Minneapolis, MN, USA) and injected subcutaneously into the flanks (3×106 cell per injection) ...
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bioRxiv - Cancer Biology 2022Quote: ... in PBS and blocked for 10 mins after which the cells were collected and resuspended in 100 µl 1% BSA/PBS containing 2 µl of antibodies SLAMF1/CD150-PE (R&D Systems) or GRPC5C Alexa Fluor-405 (R&D Systems ...
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bioRxiv - Bioengineering 2022Quote: ... A low-serum modified cell line was previously established where PANC-1 cells were cultured in DMEM supplemented with 2% FBS and 1% Insulin-Transferrin-Selenium Media Supplement (ITS, R&D Systems) (“DMEM-ITS”)45 ...
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bioRxiv - Microbiology 2022Quote: The location of DC-SIGN was assessed at the surface of BHK-21 cells (not permeabilized) by flow cytometry using an anti-DC-SIGN phycoerythrin-conjugated antibody (FAB1621P R&D Systems) according to a standard procedure (Lozach et al. ...
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bioRxiv - Neuroscience 2022Quote: ... We used 250 µg of cell lysates for each of the parts A and B of the array and followed manufacturers’ instructions (R&D Systems).
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bioRxiv - Developmental Biology 2022Quote: ... cells were incubated in cSFDM (including B27 supplement with vitamin A) with 200 ng/ml rmWNT3A (R&D Systems, #1324-WN-010), 100 ng/ml rhFGF2 (R&D Systems ...
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bioRxiv - Microbiology 2024Quote: ... the basolateral surfaces of the cells were pre-treated with blocking anti-integrin β1 antibody (25 µg/ml, R&D Systems, MAB17781) for 1h at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: Cells in 60 wells were incubated with primary goat polyclonal anti-mouse E-cadherin (R&D Systems, Cat. No AF748, 1:200) in cold 5% FBS/PBS for 20 min on ice ...
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bioRxiv - Immunology 2024Quote: ... NK cells were also co-cultured with K562 or P815 coated with anti-NCR (NKp30, NKp44, NKp46, R&D Systems, 5ug/ml) or anti-CD16 (clone 3G8 ...
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bioRxiv - Biophysics 2024Quote: Recombinant sKLA (Glu34-Ser981) with a C-terminal 6His tag expressed in a NS0 murine myeloma cell line was purchased (R&D Systems) and loaded onto a Superdex 200 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The lower compartment contained 500 μl of either cancer cell line derived conditioned medium or control media (serum free medium; 2% serum control; CCL19 (20 ng/mL, R&D Systems). The PBMCs were allowed to migrate from the upper chamber to the lower one for 3 hours ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with affinity-purified polyclonal goat anti-huACE2 antibody recognizing the extracellular domain (1:40 dilution, #AF933, R&D Systems) for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... purified hCD8+ T cells were subjected to 3 consequent cycles of αCD/αCD28 activation in the presence of 50 ng/mL TGFβ (R&D Systems) and 30U/mL rhIL-2 lasting 24h each.
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bioRxiv - Cancer Biology 2023Quote: ... 0.1 million cells (in 100 µL of 1:1 mix of PBS and Cultrex Basement Membrane Extract (Type 3, R&D systems)) were injected into the 4th mammary fat pad of 7-8 weeks old female BALB/c mice ...
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bioRxiv - Cell Biology 2023Quote: ... 1 x 106 BM cells were incubated at 37°C or 4°C for 45 min with or without Cxcl12 (10 nM, R&D Systems). The reaction was stopped by adding ice-cold RPMI and brief centrifugation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: The cells were seeded in duplicates at 5x103 in 1 ml of mouse methylcellulose complete media without Epo (HSC008; R&D Systems) containing mIL-3 ...
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bioRxiv - Cancer Biology 2023Quote: ... all cells were spun down to remove the antibody solution and resuspended in fresh flow cytometry staining buffer (R&D Systems #FC001). Cells were then passed through round bottom Falcon tubes for flow cytometric analysis using a BD LSR Fortessa.
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bioRxiv - Neuroscience 2023Quote: ... It is not uncommon for ACE2 in HEK293 cell homogenate to appear mildly elevated above 100 kDa on western blots likely due to modifications in kidney epithelial cells that are not present in ganglia (R&D Systems). The anti-NeuN antibody (ab190565) ...
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bioRxiv - Immunology 2022Quote: ... The single memory B cells were cultured in complete RPMI 1640 medium supplemented with IL-2 (10 U/ml, R&D Systems), IL-21 (10 U/ml ...
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bioRxiv - Immunology 2023Quote: ... The matured THP-1 cells were not treated or treated with 20 μg/mL poly (I:C) (4287, R&D Systems, Minneapolis, MN) for four additional hours before the cells were collected for analysis.
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bioRxiv - Microbiology 2023Quote: ... The cytokine releases were evaluated by ELISA in cell supernatants or in peritoneal fluids (IL-1β, IL-12, IL-6, TNFα, TGFβ, IL-10 BD Biosciences, R&D Systems). 8 isoprostane ...
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bioRxiv - Immunology 2024Quote: ... After overnight incubation (16-18h), cells were treated with vehicle (PBS + 1% BSA) or rmAreg (200, 500, or 1000 ng/ml) (R&D Systems). After 4h of incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... Whole cell extract was then immunoprecipitated overnight at 4°C on a rotating wheel using anti-PKR antibody (71/10, R&D Systems) conjugated to protein A sepharose beads (GE Healthcare ...
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MYB insufficiency disrupts proteostasis in hematopoietic stem cells leading to age-related neoplasiabioRxiv - Cancer Biology 2021Quote: WT and Myb+/− bone marrow cells stained for the KSL markers were incubated with 5μM Me4bodipyFL-Ahx3Leu3VS probe (R&D Systems, Minneapolis MN) for 1 hour at 37°C either with or without prior incubation for 1 hour at 37°C with 10μM of the proteosome inhibitor MG-132 (Millipore Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... The cells were then incubated overnight at 4°C in buffer G with the following antibodies: mouse monoclonal anti-Tuj1 (R&D Systems, MAB1195) at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... papillary or reticular cell sheets were seeded with HUVECs at 6 000 cells /cm2 in ECGM2ΔFGFΔVEGF supplemented or not with VEGF (R&D systems, Minneapolis MN, USA) at 5 ng/mL ...
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bioRxiv - Immunology 2020Quote: ... were seeded at a concentration of 1 × l06 cells/ml in RPMI 1640 medium supplemented with 20 ng/ml GM-CSF (415-ML-050, R&D Systems, USA). After 7 d of culture ...
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bioRxiv - Biochemistry 2021Quote: Acvr1[R206H]/+and Acvr2a −/−;Acvr2b −/−;Bmpr2 −/−;Acvr1[R206H]/+ mES cells were dissociated using non-enzymatic cell dissociation buffer (MiliPore, S-004-B) and resuspended in the flow cytometry staining buffer (R&D systems, FC001). After 15 minutes of blocking (Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were harvested after treatment and stained for PD-L1 (eBioscience, 12-5983-42) and MHC class I (R&D Systems, FAB7098G). Induction of the respective proteins was analyzed by flow cytometry.
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bioRxiv - Neuroscience 2021Quote: ... 0.5mM EDTA and 0.05% Sodium azide) for 20 minutes at 4°C following which cells were stained in suspension for anti-mouse TREM2 biotinylated (R&D systems, BAF1729) for 30 minutes at 4°C ...