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• PKR-mediated Stress Response Enhances Dengue and Zika Virus Replication

  Taissa Ricciardi-Jorge, et al., bioRxiv - Immunology 2023

  Quote: ... ISG15 mouse mAb (R&D System, MAB4845, dilution 1:1000); anti-mouse IgG ...
• Single-cell transcriptomics of adult skin VE-cadherin expressing lineages during hair cycle

  Gopal Chovatiya, et al., bioRxiv - Cell Biology 2023

  Quote: ... VE-cadherin (1:500, R&D Systems, AF1002); LYVE1 (1:400 ...
• Deep mutational scanning highlights a new role for cytosolic regions in Hrd1 function

  Brian G. Peterson, et al., bioRxiv - Biochemistry 2023

  Quote: ... 125 μM ubiquitin (yeast recombinant from bio-techne (R&D Systems)) ...
• Single cell transcriptomic landscapes of human liver organoids stratify models of non-alcoholic fatty liver disease

  Anja Hess, et al., bioRxiv - Molecular Biology 2023

  Quote: ... were prepared to obtain solutions of TGF-β1 (10 ng/mL and 25 ng/mL, R&D Systems 240-B-002), palmitic acid (PA ...
• Phencyclidine disrupts neural coordination and cognitive control by dysregulating translation

  Eun Hye Park, et al., bioRxiv - Neuroscience 2023

  Quote: ... Ro 25-6981 maleate (R&D Systems, Cat.No. 1594), and NVP-AAM077/PEAQX (Sigma ...
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: ... All of the cytokines were purchased from R&D Systems.
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: ... cells were fed with 10 ng/mL of neurotrophic factors (BDNF, GDNF, IGF-1 from R&D systems, NT3 from Stem Cell Technologies), ascorbic acid (2.27 µM) ...
• Cytokine signaling converging on IL11 in ILD fibroblasts provokes aberrant epithelial differentiation signatures

  Miriam T. Kastlmeier, et al., bioRxiv - Cell Biology 2023

  Quote: ... and Albumin (ALB, R&D Systems). NKX2.1GFP+ lung progenitor cells were enriched by GFP signal for NKX2.1 based on a previously described protocol ...
• The enigma of the SARS-CoV-2 microcirculation dysfunction: evidence for modified endothelial junctions

  L Bouillet, et al., bioRxiv - Biochemistry 2023

  Quote: Recombinant Human ACE2 Protein (933-ZN) was purchased from R&D Systems (Minneapolis, MN, U.S.A.) and stored in a buffer according to the manufacturer’s instructions ...
• The enigma of the SARS-CoV-2 microcirculation dysfunction: evidence for modified endothelial junctions

  L Bouillet, et al., bioRxiv - Biochemistry 2023

  Quote: ... Monoclonal mouse anti-human ACE2 Antibody (MAB933) was purchased from R&D Systems (Minneapolis, MN, U.S.A.). VE-cadherin extracellular domain (EC1-5 ...
• Golgi organization is a determinant of stem cell function in the small intestine

  Sandra Scharaw, et al., bioRxiv - Cell Biology 2023

  Quote: ... 500 ng/ml R-spondin-1 (R&D Systems), 1 x B-27 (Life Technologies) ...
• Golgi organization is a determinant of stem cell function in the small intestine

  Sandra Scharaw, et al., bioRxiv - Cell Biology 2023

  Quote: ... except for mouse Egf (R&D Systems) added at 200 ng/ml ...
• Golgi organization is a determinant of stem cell function in the small intestine

  Sandra Scharaw, et al., bioRxiv - Cell Biology 2023

  Quote: ... 50 ng/ml mouse Egf (R&D Systems), 100 ng/ml Noggin (Peprotech) ...
• Overexpressed Malat1 Drives Metastasis through Inflammatory Reprogramming of Lung Adenocarcinoma Microenvironment

  Elena Martinez-Terroba, et al., bioRxiv - Cancer Biology 2023

  Quote: ... and Proteome Profiler Human Protease Array Kit (R&D systems) according to the manufacturer’s instructions ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 10 nM gastrin (R&D Systems), 10 nM nicotinamide (Sigma-Aldrich) ...
• Meningeal origins and dynamics of perivascular fibroblast development on the mouse cerebral vasculature

  Hannah E Jones, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and goat anti-CD206 (R&D Systems, Cat#: AF2535). Appropriate species-specific Alexa-Fluor secondary antibodies (Invitrogen ...
• Involvement of an IgE/Mast cell/B cell amplification loop in abdominal aortic aneurysm progression

  Alexia Loste, et al., bioRxiv - Pathology 2023

  Quote: The ROSAKIT WT human cell line (RRID:CVCL_5G49) was cultured in the presence of recombinant human SCF (80 ng/mL, R&D Systems), as described previously (23) ...
• Involvement of an IgE/Mast cell/B cell amplification loop in abdominal aortic aneurysm progression

  Alexia Loste, et al., bioRxiv - Pathology 2023

  Quote: ... sections were incubated with retrieval reagent (R&D Systems), then immunostained using antibodies against IgE (goat polyclonal ...
• A common human MLKL polymorphism confers resistance to negative regulation by phosphorylation

  Sarah E. Garnish, et al., bioRxiv - Immunology 2023

  Quote: ... and 30 ng/ml mouse IFNβ (R&D Systems #8234-MB-010)
• Dissecting the functional contributions of different neuronal subtypes in the dorsal striatum to perseverative behaviour in ephrin-A2/A5-/- mice

  Maitri Tomar, Jennifer Rodger, Jessica Moretti, bioRxiv - Neuroscience 2023

  Quote: ... and glutamate decarboxylase 67 (GAD67) (anti-Goat, 1:750, R&D systems, AF2086-SP), a marker for GABAergic neurons (Lazarus et al. ...
• CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death

  Neil Kuehnle, et al., bioRxiv - Microbiology 2023

  Quote: ... The included recombinant human TRAIL standard (R&D Systems, 892375) was serially diluted in 2-fold steps from 1000 to 15.6 pg/mL ...
• CRISPR Screens Identify Novel Regulators of cFLIP Dependency and Ligand-Independent, TRAIL-R1-Mediated Cell Death

  Neil Kuehnle, et al., bioRxiv - Microbiology 2023

  Quote: Anti-TRAIL ELISA was performed using a Human TRAIL/TNFSF10 Quantikine ELISA Kit (R&D Systems, DTRL00) following manufacturer-provided protocols ...
• Th1 cells alter the inflammatory signature of IL-6 by channeling STAT transcription factors to Alu-like retroelements

  David Millrine, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were cultured for 4 days in the presence of 20ng/ml murine recombinant IL-12 (R&D systems; 419-ML). The proportion of IFNγ-secreting CD4+ T-cells (Th1 cells ...
• Isthmus progenitor cells contribute to homeostatic cellular turnover and support regeneration following intestinal injury

  E. Malagola, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Rspo1 (R&D Systems, 3474-RS-050) supplemented with CHIR (Sigma-Aldrich ...
• SARS-CoV-2 Infects Peripheral and Central Neurons Before Viremia, Facilitated by Neuropilin-1

  Jonathan D. Joyce, et al., bioRxiv - Neuroscience 2023

  Quote: ... It is not uncommon for ACE2 in HEK293 cell homogenate to appear mildly elevated above 100 kDa on western blots likely due to modifications in kidney epithelial cells that are not present in ganglia (R&D Systems). The anti-NeuN antibody (ab190565) ...
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: pH 6.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), Aggrecan (Millipore ...
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: ... PROX1 (R&D systems; AF2727; 1:200), and DPEAAE (cleaved-versican ...
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: pH 9.0 Tris-EDTA buffer was used as the antigen-retrieval buffer for the following antibodies: CD31 (R&D systems; AF3628; 1:500), SOX9 (Millipore ...
• Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung

  Tristan Frum, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 100 ng/mL rhTGFβ1 (R&D Systems Cat#240-B-002), 100 ng/mL rhNOGGIN (produced in-house ...
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: ... (R&D systems; AF743; 1:400) and GFP (Abcam ...
• Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung

  Tristan Frum, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 10ng/mL recombinant human FGF7 (R&D Systems, Cat#251-KG), and 3µM CHIR99021 (APExBIO ...
• PROX1 inhibits PDGF-B expression to prevent myxomatous degeneration of heart valves

  Yen-Chun Ho, et al., bioRxiv - Developmental Biology 2023

  Quote: ... PROX1 (R&D systems; AF2727; 1:200), PROX1 (AngioBio ...
• Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung

  Tristan Frum, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 100 ng/mL rhNOGGIN (produced in-house) or 100 ng/mL BMP4 (R&D Systems Cat#314-BP-050) was added to human lung ALI explant media.
• Age and Sex-Dependent Differences in Human Cardiac Matrix-Bound Exosomes Modulate Fibrosis through Synergistic miRNA Effects

  George Ronan, et al., bioRxiv - Bioengineering 2023

  Quote: ... The lysed LVV solution was assessed using the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems) as described previously76 ...
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and IL-1β ELISA kit (DY401-05, R&D Systems Minneapolis MN). Plates were read using the Biotek Synergy HTX plate reader (Gen 5 software) ...
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-E-cadherin (1:400; R&D Systems; MAB18381) or biotinylated-WGA Lectin (1:1000 ...
• Loss of polarity regulators initiates gasdermin E mediated pyroptosis in human maternal fetal interface trophoblasts

  Khushali Patel, et al., bioRxiv - Developmental Biology 2023

  Quote: ... anti-EEA1 (1:50; R&D Systems MAB8047); anti-cleaved GSDMD Asp275 (1:500 ...
• Lysosomal cystine export regulates mTORC1 signaling to guide kidney epithelial cell fate specialization

  Marine Berquez, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-Lipoclain2/NGAL (R&D systems, AF1857, 1:400), anti-cleaved caspase 3 (Cell signaling) ...
• Ultrastructural comparison of human kidney organoids and human fetal kidneys reveals features of hyperglycemic culture

  Anika Schumacher, et al., bioRxiv - Bioengineering 2023

  Quote: ... 8 μM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/ml fibroblast growth factor 9 (FGF9 ...
• Autophagy is required to prevent early pro-inflammatory responses and neutrophil recruitment during Mycobacterium tuberculosis infection without affecting pathogen replication in macrophages

  Rachel L. Kinsella, et al., bioRxiv - Immunology 2023

  Quote: ... ELISAs were performed according to the manufacturer’s instructions (R&D systems): KC/CXCL1 (DY453) ...
• Ultrastructural comparison of human kidney organoids and human fetal kidneys reveals features of hyperglycemic culture

  Anika Schumacher, et al., bioRxiv - Bioengineering 2023

  Quote: ... 200 ng/ml fibroblast growth factor 9 (FGF9; R&D Systems), and 1 μg/ml heparin (Sigma-Aldrich ...
• The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection

  Déborah Lécuyer, et al., bioRxiv - Microbiology 2023

  Quote: ... Primary anti-human ACE-2 antibody (#AF933, R&D systems) or isotype-matched antibody (#AB-108-C ...
• A Resource of Gene Expression Data from a Multiethnic Population Cohort of Induced Pluripotent Cell–Derived Cardiomyocytes

  Wenjian Lv, et al., bioRxiv - Genomics 2023

  Quote: ... followed by recombinant human BMP-4 (5 ng/mL; R&D Systems) and CHIR 99021 (1 nM ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mLamp-1 (1:1000, AF4320, R&D Systems), anti-Phospho-p38 (1:1000 ...
• Stricturing Crohn’s disease single-cell RNA sequencing reveals fibroblast heterogeneity and intercellular interactions

  Pranab K. Mukherjee, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human CDH11-Fc chimera (CDH11Fc, R&D Systems; Cat# 1970-CA) or H1M1 antibody (prepared from hybridoma PTA-9699 (ATCC ...
• Microbiome ssRNA as an environmental cue to activate TLR13-dependent tissue-protective programs in CD5L^hi hepatic macrophages

  Ryota Sato, et al., bioRxiv - Immunology 2023

  Quote: ... antibody was purchased from Invitrogen and monoclonal anti-mouse Axl antibody (clone 175128) from R&D Systems (Minneapolis, MN, USA). Polyclonal anti-mouse LXRα antibody (#ab3585 ...
• Fluctuation of lysosomal protein degradation in neural stem cells of postnatal mouse brain

  He Zhang, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-Sox2 (R&D Systems, AF2018), chicken anti-Nestin (aves ...
• Adipocytes regulate fibroblast function, and their loss contributes to fibroblast dysfunction in inflammatory diseases

  Heather J. Faust, et al., bioRxiv - Cell Biology 2023

  Quote: ... or COL1A1 (R&D Systems, AF6220). Secondary antibodies were applied at room temperature for 1.5 hours (donkey anti–rat AF488 ...
• EOGT Enables Residual Notch Signaling in Mouse Intestinal Cells Lacking POFUT1

  Mohd Nauman, et al., bioRxiv - Developmental Biology 2023

  Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
• Osmolar modulation drives reversible cell cycle exit and human pluripotent cell differentiation via NF-κВ and WNT signaling

  Jonathan Sai-Hong Chui, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 1% Endothelial Cell Growth Supplement (ECGS, R&D systems, 390599) was added to the LDM medium ...