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• GLI1 facilitates rheumatoid arthritis by collaborative regulation of DNA methyltransferases

  Gaoran Ge, et al., bioRxiv - Cell Biology 2023

  Quote: ... 30 ng/ml macrophage colony stimulating factor (M-CSF; R&D Systems, Minnesota, USA) and 100 U/ml penicillin/streptomycin (P/S ...
• CDK5 activity in retinal pigment epithelium contributes to gap junction dynamics during phagocytosis

  Julia Fadjukov, et al., bioRxiv - Cell Biology 2023

  Quote: ... the AICS-mGJA1 and hESC inserts were incubated either with 100 µM roscovitine (Cat. no. 1332, R&D Systems) or equivalent amount of DMSO for 0 – 24h ...
• Mechanical forces at the kidney filtration barrier govern spatial orientation of podocyte processes on capillaries

  David Unnersjö-Jess, et al., bioRxiv - Cell Biology 2023

  Quote: ... Primary antibodies used: Sheep anti-nephrin (R&D Systems AF4269 1:50), mouse anti-acetylated tubulin (SIGMA T6793 1:100) ...
• Recruitment of the SNX17-Retriever recycling pathway regulates synaptic function and plasticity

  Pilar Rivero-Ríos, et al., bioRxiv - Cell Biology 2023

  Quote: ... mouse/rabbit Integrin β1 goat pAb (1:1000, AF2405, R&D Systems) and GAPDH rabbit mAb (1:2000 ...
• A Soluble Platelet-Derived Growth Factor Receptor-β Originates via Pre-mRNA Splicing in the Healthy Brain and is Differentially Regulated during Hypoxia and Aging

  Laura Beth Payne, et al., bioRxiv - Cell Biology 2023

  Quote: ... goat anti-CD31/platelet-endothelial cell adhesion molecule-1 (PECAM-1) antibody (R&D Systems, AF3628,1:500), and rabbit anti-glial-fibrillary acidic protein (GFAP ...
• Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

  Mary E. Mohr, et al., bioRxiv - Cell Biology 2023

  Quote: ... PDGFRα (1:100, R&D Systems, AF1062-SP), MF20 (1:400 ...
• Cardiomyocyte-fibroblast interaction regulates ferroptosis and fibrosis after myocardial injury

  Mary E. Mohr, et al., bioRxiv - Cell Biology 2023

  Quote: HCF-conditioned media were applied to the Proteome Profiler Human XL Cytokine Array Kit (R&D Systems, ARY022B). 5ml of conditioned medium from H2O2 or H2O treated HCFs was concentrated into 500μl using MilliporeSigma Protein-Concentrate Kit (Fisher Scientific ...
• Single shot dendritic cell targeting SARS-CoV-2 vaccine candidate induces broad and durable systemic and mucosal immune responses

  Nicholas You Zhi Cheang, et al., bioRxiv - Immunology 2023

  Quote: ... Using a SARS-CoV-2 spike RBD mAb of known concentration (MAB10580; R&D Systems, Minneapolis, Minnesota, USA) as a molecular standard ...
• Targeting the MR1-MAIT Cell Axis Improves Vaccine Efficacy and Affords Protection against Viral Pathogens

  Rasheduzzaman Rashu, et al., bioRxiv - Immunology 2023

  Quote: ... plus 5 ng/mL of recombinant mouse IL-18 (R&D Systems). To retain intracellular mediators for downstream cytofluorimetric analyses ...
• Tozorakimab (MEDI3506): a dual-pharmacology anti-IL-33 antibody that inhibits IL-33 signalling via ST2 and RAGE/EGFR to reduce inflammation and epithelial dysfunction

  Elizabeth England, et al., bioRxiv - Immunology 2023

  Quote: ... Samples were analysed using the human IL-33 duoset (R&D Systems) and the IL33004-IL330425-biotin assays (AstraZeneca) ...
• Targeting the MR1-MAIT Cell Axis Improves Vaccine Efficacy and Affords Protection against Viral Pathogens

  Rasheduzzaman Rashu, et al., bioRxiv - Immunology 2023

  Quote: ... supplemented with 20 ng/mL of recombinant mouse interleukin (IL)- 2 (R&D systems), 8% CTS Immune Cell Serum Replacement (Thermo Fisher) ...
• Intraperitoneal transfer of wild-type bone marrow cells in the Csf1r knockout rat repopulates resident tissue macrophages without contributing to monocytopoiesis

  Anuj Sehgal, et al., bioRxiv - Immunology 2023

  Quote: Serum IGF1 was measured using a Mouse/Rat DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions as previously described [14] ...
• Butyrate, valerate, and niacin ameliorate anaphylaxis by suppressing IgE-dependent mast cell activation: Roles of GPR109A, PGE₂, and epigenetic regulation

  Kazuki Nagata, et al., bioRxiv - Immunology 2023

  Quote: The concentrations of IL-13 and TNF-α in the culture media were measured by using mouse IL-13 DuoSet ELISA (R&D Systems, Minneapolis, MN) and ELISA MAX Deluxe Set mouse TNF-α (BioLegend) ...
• Kaempferol suppresses the activation of mast cells by modulating the expression of FcεRI and SHIP1

  Kazuki Nagata, et al., bioRxiv - Immunology 2023

  Quote: ... and of IL-13 was determined using the mouse IL-13 DuoSet ELISA kit (DY413, R&D systems).
• An IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma

  Madelon M.E. de Jong, et al., bioRxiv - Immunology 2023

  Quote: ... slides were incubated overnight with anti-human IL-1β (1:100, 615417, R&D Systems) at room temperature ...
• An IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma

  Madelon M.E. de Jong, et al., bioRxiv - Immunology 2023

  Quote: ... with or without 200ng/mL recombinant human BAFF (R&D Systems). After overnight culture ...
• An IL-1β driven neutrophil-stromal cell axis fosters a BAFF-rich microenvironment in multiple myeloma

  Madelon M.E. de Jong, et al., bioRxiv - Immunology 2023

  Quote: ... Levels of BAFF protein were determined in culture supernatant or bone marrow plasma using the human BAFF/BLyS/TNFSF13B DuoSet ELISA kit (R&D Systems) in combination with the Pierce Streptavidin-Poly-HRP (1:10,000 ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: Commercially available kits and manufacturer protocols from R&D Systems (mCsf2; DY416) were employed for ELISA ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse IL17ra-APC (clone 657643, R&D systems), anti-mouse T1/St2-FITC (clone DJ8 ...
• Primary lung fibroblasts respond to IL-33, IL-13, and IL-17A by secreting factors that activate macrophages

  Jarrett Venezia, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mouse Csf2ra-Alexa 700 (clone 698423, R&D systems), or anti-mouse CD11b-BV785 (clone M1/70 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... IL-18 (20 ng/ml 9124-IL-010; R&D Systems), IL-23 (20 ng/ml ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human CCL2 (100 ng/ml, 279-MC-010; R&D Systems) was added to the cells immediately before loading them into the flow chamber and the CCL2 concentration was maintained during the initial 4 min of the assay.
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... were coated with fibronectin (50 μg/ml, 1918-FN; R&D Systems) in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... in PBS and HUVECs were plated at confluence and were stimulated with 40 ng/ml recombinant human TNF-α (210-TA; R&D Systems) and/or 50 ng/ml IFN-γ (285-IF ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... Concentrations of chemokines were determined using ELISA kits from R&D Systems (CCL2 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... and TNF-α (20 ng/ml 210-TA-005/CF; R&D Systems) in media containing 10 ng/ml IL-7 (BT-007-010 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... and/or 50 ng/ml IFN-γ (285-IF; R&D Systems) for 18–20 h at 37°C in 5% CO2 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... T cell were pre-incubated with 1 mg/ml pertussis toxin (3097; R&D Systems) in RPMI-10 medium for 3 h at 37°C ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... supernatants were collected and concentrations of IFN-γ and GM-CSF were determined using ELISA kits from R&D Systems (IFN-γ; DIF50C, GM-CSF; DGM00) according to the manufacturer’s instructions.
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... HUVEC monolayers in chambers slides were pre-treated with 20 mg/ml anti-human CCL20/MIP-3α antibody (MAB360; R&D Systems) for 2 h at 37°C ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... IL-1β (20 ng/ml 201-LB-005/CF; R&D Systems) and TNF-α (20 ng/ml 210-TA-005/CF ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: PBMC were isolated from whole blood and treated with recombinant human CCL2 (279-MC-010; R&D Systems) at 100 ng/ml ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... Purified cells from five subsets were cultured in IL-12 (20 ng/ml, 219-IL-005; R&D Systems), IL-18 (20 ng/ml 9124-IL-010 ...
• Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells

  Farhat Parween, et al., bioRxiv - Immunology 2023

  Quote: ... IL-23 (20 ng/ml, 1290-IL-010/CF; R&D Systems), IL-1β (20 ng/ml 201-LB-005/CF ...
• The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspases

  Patrick M. Exconde, et al., bioRxiv - Immunology 2023

  Quote: ... hIL-1β Goat Polyclonal Ab (R&D systems, AF-201-NA), hIL-18 Goat Ab (R&D systems ...
• The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspases

  Patrick M. Exconde, et al., bioRxiv - Immunology 2023

  Quote: ... hIL-18 Goat Ab (R&D systems, af2548), hIL-1α Recombinant Ab (PeproTech ...
• Inflammation in the COVID-19 airway is due to inhibition of CFTR signaling by the SARS-CoV-2 Spike protein

  Hung Caohuy, et al., bioRxiv - Microbiology 2023

  Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
• Multi-omic network analysis identified betacellulin as a novel target of omega-3 fatty acid attenuation of western diet-induced nonalcoholic steatohepatitis

  Jyothi Padiadpu, et al., bioRxiv - Systems Biology 2023

  Quote: ... BTC at 40 ng/mL (human recombinant Betacellulin protein, R&D systems, MN), or combinations of all for 6 hours ...
• Single-cell transcriptomics of resected human traumatic brain injury tissues reveals acute activation of endogenous retroviruses in oligodendroglia

  Raquel Garza, et al., bioRxiv - Neuroscience 2023

  Quote: ... OLIG2 (R&D Systems, AF2418, 1:500), OLIG2 (Sigma-Aldrich ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: ... either 5μg or 2.5μg of the S protein or NC protein (R&D Systems, cat# 10474-CV-050 and cat# 10549-CV-100) ...
• A novel biopolymer for mucosal adjuvant against respiratory pathogens

  Ashley R. Hoover, et al., bioRxiv - Bioengineering 2023

  Quote: Recombinant SARS-CoV-2 proteins were purchased from R&D systems. For vaccination ...
• Interferon-γ Elicits Pathological Hallmarks of ALS in Human Motor Neurons

  Changho Chun, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-ChAT (1 in 200, R&D Systems), Alexafluor-488 conjugated goat-anti-mouse secondary antibody (1:500 ...
• Identification of host-interacting domains of surface layer protein B from Levilactobacillus brevis and the evidence as glycoprotein

  Zheng Lin Tan, Naoyuki Yamamoto, bioRxiv - Biochemistry 2023

  Quote: ... enzyme linked immunosorbent assay (ELISA) kit for IL-12/IL-23 (p40) was obtained from R&D Systems (Minnesota, USA); ELISA kit for IL-6 ...
• Reactive astrocytes associated with prion disease impair the blood brain barrier

  Rajesh Kushwaha, et al., bioRxiv - Neuroscience 2023

  Quote: ... ELISA kit for IL-6 and recombinant mouse IL-6 wrtr from R&D Systems (Minneapolis, MN). Bicinchoninicacid (BCA ...
• Oligodendrocytes in human iPS cell-derived cortical grafts remyelinate adult rat and human cortical neurons

  Raquel Martinez-Curiel, et al., bioRxiv - Neuroscience 2023

  Quote: ... member 3A (Wnt3A) (10 ng/mL, R&D Systems) and cyclopamine (1 μM ...
• Oligodendrocytes in human iPS cell-derived cortical grafts remyelinate adult rat and human cortical neurons

  Raquel Martinez-Curiel, et al., bioRxiv - Neuroscience 2023

  Quote: ... and B27 were omitted and lt-NES cells were cultured at low density in a differentiation-defined medium (DDM) in the presence of bone morphogenetic protein 4 (BMP4) (10 ng/mL, R&D Systems), wingless-type MMTV integration site family ...
• SARS-CoV-2 Infects Peripheral and Central Neurons Before Viremia, Facilitated by Neuropilin-1

  Jonathan D. Joyce, et al., bioRxiv - Neuroscience 2023

  Quote: ... Neuropilin-1 was visualized using a goat polyclonal anti-NRP-1 antibody at a 15 µg/mL concentration (AF566; R&D Systems) followed by an Alexa Fluor® 647 conjugated donkey anti-goat monoclonal antibody at a 1:1000 concentration (ab150135 ...
• Reactive astrocytes associated with prion disease impair the blood brain barrier

  Rajesh Kushwaha, et al., bioRxiv - Neuroscience 2023

  Quote: The IL-6 levels in astrocytes conditioned medium (ACM) were determined using the IL-6 ELISA kit per the manufacturer’s instruction (R&D Systems, Minneapolis, MN). Briefly ...
• Hypothesis: Colony-forming activity of pluripotent stem cell-derived hepatocyte-like cells for stem cell assay

  Kenta Ite, et al., bioRxiv - Cell Biology 2023

  Quote: ... 10 ng/mL heregulin-1β (recombinant human NRG-beta 1/HRG-beta 1 EGF domain; R&D Systems, Minneapolis, MN, USA), 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1 ...