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• Ultrastructural comparison of human kidney organoids and human fetal kidneys reveals features of hyperglycemic culture

  Anika Schumacher, et al., bioRxiv - Bioengineering 2023

  Quote: ... 8 μM GSK-3 inhibitor CHIR99021 (R&D Systems), 200 ng/ml fibroblast growth factor 9 (FGF9 ...
• Autophagy is required to prevent early pro-inflammatory responses and neutrophil recruitment during Mycobacterium tuberculosis infection without affecting pathogen replication in macrophages

  Rachel L. Kinsella, et al., bioRxiv - Immunology 2023

  Quote: ... ELISAs were performed according to the manufacturer’s instructions (R&D systems): KC/CXCL1 (DY453) ...
• Ultrastructural comparison of human kidney organoids and human fetal kidneys reveals features of hyperglycemic culture

  Anika Schumacher, et al., bioRxiv - Bioengineering 2023

  Quote: ... 200 ng/ml fibroblast growth factor 9 (FGF9; R&D Systems), and 1 μg/ml heparin (Sigma-Aldrich ...
• The purinergic receptor P2X7 and the NLRP3 inflammasome are druggable host factors required for SARS-CoV-2 infection

  Déborah Lécuyer, et al., bioRxiv - Microbiology 2023

  Quote: ... Primary anti-human ACE-2 antibody (#AF933, R&D systems) or isotype-matched antibody (#AB-108-C ...
• A Resource of Gene Expression Data from a Multiethnic Population Cohort of Induced Pluripotent Cell–Derived Cardiomyocytes

  Wenjian Lv, et al., bioRxiv - Genomics 2023

  Quote: ... followed by recombinant human BMP-4 (5 ng/mL; R&D Systems) and CHIR 99021 (1 nM ...
• Endosome dysfunction leads to gain-of-function TLR7 and human lupus

  Harshita Mishra, et al., bioRxiv - Immunology 2023

  Quote: ... anti-mLamp-1 (1:1000, AF4320, R&D Systems), anti-Phospho-p38 (1:1000 ...
• Stricturing Crohn’s disease single-cell RNA sequencing reveals fibroblast heterogeneity and intercellular interactions

  Pranab K. Mukherjee, et al., bioRxiv - Immunology 2023

  Quote: ... recombinant human CDH11-Fc chimera (CDH11Fc, R&D Systems; Cat# 1970-CA) or H1M1 antibody (prepared from hybridoma PTA-9699 (ATCC ...
• Microbiome ssRNA as an environmental cue to activate TLR13-dependent tissue-protective programs in CD5L^hi hepatic macrophages

  Ryota Sato, et al., bioRxiv - Immunology 2023

  Quote: ... antibody was purchased from Invitrogen and monoclonal anti-mouse Axl antibody (clone 175128) from R&D Systems (Minneapolis, MN, USA). Polyclonal anti-mouse LXRα antibody (#ab3585 ...
• Fluctuation of lysosomal protein degradation in neural stem cells of postnatal mouse brain

  He Zhang, et al., bioRxiv - Neuroscience 2023

  Quote: ... goat anti-Sox2 (R&D Systems, AF2018), chicken anti-Nestin (aves ...
• Adipocytes regulate fibroblast function, and their loss contributes to fibroblast dysfunction in inflammatory diseases

  Heather J. Faust, et al., bioRxiv - Cell Biology 2023

  Quote: ... or COL1A1 (R&D Systems, AF6220). Secondary antibodies were applied at room temperature for 1.5 hours (donkey anti–rat AF488 ...
• EOGT Enables Residual Notch Signaling in Mouse Intestinal Cells Lacking POFUT1

  Mohd Nauman, et al., bioRxiv - Developmental Biology 2023

  Quote: Flow cytometry performed within 1-6 days investigated NOTCH1 expression using anti-NECD1 antibody AF5267 and Notch ligand binding for DLL1-Fc (R&D Systems, Inc., Minneapolis, MN) and DLL4-Fc (Aro Biosystems ...
• Osmolar modulation drives reversible cell cycle exit and human pluripotent cell differentiation via NF-κВ and WNT signaling

  Jonathan Sai-Hong Chui, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 1% Endothelial Cell Growth Supplement (ECGS, R&D systems, 390599) was added to the LDM medium ...
• Conserved cardiolipin-mitochondrial ADP/ATP carrier interactions assume distinct structural and functional roles that are clinically relevant

  Nanami Senoo, et al., bioRxiv - Biochemistry 2023

  Quote: ... were grown on a plate coated by Cultrex Rat Collagen I (50 µg/ml, R&D systems 3440-100-01) at 37°C in 5% CO2 in high glucose-containing DMEM (Corning 10-013-CM ...
• Myosin inhibitor reverses hypertrophic cardiomyopathy in pediatric iPSC-cardiomyocytes to mirror variant correction

  Caroline Kinnear, et al., bioRxiv - Genetics 2023

  Quote: ... or anti-MYH7 antibody (1:500, R&D systems, Minneapolis, MN) by shaking gently overnight at 4 °C ...
• Sex biased human thymic architecture guides T cell development through spatially defined niches

  Laura N Stankiewicz, et al., bioRxiv - Immunology 2023

  Quote: ... DLL1-Fc (R&D Systems), or DLL4-Fc (Sino Biological ...
• Sex biased human thymic architecture guides T cell development through spatially defined niches

  Laura N Stankiewicz, et al., bioRxiv - Immunology 2023

  Quote: ... were diluted to 4 µg/ml in PBS along with 1 µg/ml VCAM-1-Fc (R&D Systems). To coat ...
• Opposing roles for TGFβ- and BMP-signaling during nascent alveolar differentiation in the developing human lung

  Tristan Frum, et al., bioRxiv - Developmental Biology 2023

  Quote: ... 50 ng/mL rhEGF (R&D Systems, Cat#236-EG) with (SFFF ...
• Molecular signatures associated with successful implantation of the human blastocyst

  Jennifer N. Chousal, et al., bioRxiv - Developmental Biology 2023

  Quote: ... and anti-GATA-6 (R&D Systems, AF1700) was used at a dilution of 1:500) ...
• A Resource of Gene Expression Data from a Multiethnic Population Cohort of Induced Pluripotent Cell–Derived Cardiomyocytes

  Wenjian Lv, et al., bioRxiv - Genomics 2023

  Quote: ... (Thermo Fisher Scientific)] medium supplemented with recombinant human/mouse/rat activin A (100 ng/mL; R&D Systems) for 18 hours ...
• Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis

  Charlotte R. Wayne, et al., bioRxiv - Neuroscience 2023

  Quote: ... and TNF (R&D Systems, 410-MT), which were added at 10ng/mL ...
• Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis

  Charlotte R. Wayne, et al., bioRxiv - Neuroscience 2023

  Quote: ... Media containing either mouse IFNγ (R&D Systems, 485-MI-100), mouse IL-17A (R&D Systems ...
• Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis

  Charlotte R. Wayne, et al., bioRxiv - Neuroscience 2023

  Quote: ... mouse IL-17A (R&D Systems, 7956-ML-025), mouse GM-CSF (R&D Systems ...
• Distinct Th17 effector cytokines differentially promote microglial and blood-brain barrier inflammatory responses during post-infectious encephalitis

  Charlotte R. Wayne, et al., bioRxiv - Neuroscience 2023

  Quote: ... except IL-1β (R&D Systems, 401-ML) and TNF (R&D Systems ...
• Genipin Crosslinks the Extracellular Matrix to Rescue Developmental and Degenerative Defects, and Accelerates Regeneration of Peripheral Neurons

  Kenyi Saito-Diaz, et al., bioRxiv - Neuroscience 2023

  Quote: ... 1614)+300 nM CHIR99021 (R&D Systems, 4423). On day two ...
• Genipin Crosslinks the Extracellular Matrix to Rescue Developmental and Degenerative Defects, and Accelerates Regeneration of Peripheral Neurons

  Kenyi Saito-Diaz, et al., bioRxiv - Neuroscience 2023

  Quote: ... 314-BP)+10 μM SB431542 (R&D Systems, 1614)+300 nM CHIR99021 (R&D Systems ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 10 nM SB202190 (R&D Systems), 10 nM gastrin (R&D Systems) ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 50 ng/mL recombinant human EGF (R&D Systems), 2% Noggin/R-Spondin–conditioned media (R&D Systems) ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... advanced DMEM/F12 containing R-spondin1 (R&D Systems), Noggin (Pepro Tech ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 100 ng/mL recombinant human Wnt3A (R&D Systems), 500 nM A83-01 (R&D Systems) ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... which were containing advanced DMEM/F12 with R-spondin1 (R&D Systems), Noggin (Pepro Tech ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 2% Noggin/R-Spondin–conditioned media (R&D Systems), 100 ng/mL recombinant human Wnt3A (R&D Systems) ...
• Mammalian Esophageal Stratified Tissue Homeostasis is Maintained Distinctively by the Epithelial Pluripotent p63+Sox2+ and p63-Sox2+ Cell Populations

  Xiaohong Yu, et al., bioRxiv - Cell Biology 2023

  Quote: ... 500 nM A83-01 (R&D Systems), 10 nM SB202190 (R&D Systems) ...
• Memory Th1 cells modulate heterologous diseases through innate function

  NIkolas Rakebrandt, et al., bioRxiv - Immunology 2023

  Quote: ... 100 µg of anti-CXCL16 antibody (clone 142417; R&D Systems) was injected i.v ...
• Cell targeting and immunostimulatory properties of a novel Fcγ-receptor independent agonistic anti-CD40 antibody in rhesus macaques

  Xianglei Yan, et al., bioRxiv - Immunology 2023

  Quote: ELISA plates were coated with 2 µg/ml mouse anti-His tag antibody (R&D Systems, MAB050) and blocked as described above ...
• Memory Th1 cells modulate heterologous diseases through innate function

  NIkolas Rakebrandt, et al., bioRxiv - Immunology 2023

  Quote: ... together with 20 µg anti-IL18R antibody (clone 112624; R&D Systems) or 60 µg of anti-IL-12p40 antibody (clone C17.8 ...
• Genomic transcription factor binding site selection is edited by the chromatin remodeling factor CHD4

  Mika Saotome, et al., bioRxiv - Genomics 2023

  Quote: ... MDA-MB-231 cells were cultured in DMEM high-glucose medium with 10% FBS (Thermo Fisher Scientific or R&D Systems). Doxycycline-inducible GATA3 expression system in MDA-MB-231 cells was developed by lentiviral transduction ...
• A cis-regulatory element regulates ERAP2 expression through autoimmune disease risk SNPs

  Wouter J. Venema, et al., bioRxiv - Genomics 2023

  Quote: ... and anti-ERAP2 (AF3830, R&D Systems). Beads were washed in assay buffer (50 mM Tris ...
• Apical expansion of calvarial osteoblasts and suture patency is dependent on graded fibronectin cues

  Xiaotian Feng, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Goat anti-Runx 2 (1:100; R&D Systems AF2006-SP; RRID:AB_2184528) and mouse anti-GM130 (1:100 ...
• Eomes restricts Brachyury functions at the onset of mammalian gastrulation

  Katrin M. Schüle, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Primary antibodies used were anti-brachyury (R&D Systems; AF2085, 1:500), anti-Eomes (abcam ...
• Control of gastruloid patterning and morphogenesis by the Erk and Akt signaling pathways

  Evan J. Underhill, Jared E. Toettcher, bioRxiv - Developmental Biology 2023

  Quote: ... and Brachyury goat (R&D Systems, AF2085 ...
• An Antibody-Based Molecular Switch for Continuous Biosensing

  Ian A.P. Thompson, et al., bioRxiv - Bioengineering 2023

  Quote: ... was obtained from Sigma Aldrich and digoxigenin monoclonal antibody (Clone # 611621) was obtained from R&D Systems. T4 Ligase and low molecular-weight DNA ladder were obtained from New England Biolabs (NEB) ...
• Therapeutic immune cell engineering with an mRNA : AAV-Sleeping Beauty composite system

  Lupeng Ye, et al., bioRxiv - Bioengineering 2023

  Quote: ... Cells were incubated with CD22-Fc protein (R&D Systems) in PBS for 30 min on ice and washed with PBS twice to remove the unbonded protein ...
• Therapeutic immune cell engineering with an mRNA : AAV-Sleeping Beauty composite system

  Lupeng Ye, et al., bioRxiv - Bioengineering 2023

  Quote: ... cells were incubated with CD22-Fc or BCMA-Fc protein (R&D Systems) in PBS for 30 min on ice ...
• Self-organization, quality control, and preclinical studies of human iPSC-derived retinal sheets for tissue-transplantation therapy

  Kenji Watari, et al., bioRxiv - Neuroscience 2023

  Quote: ... anti-NRL (goat; 1:500; R&D Systems), anti-Lhx2 (rabbit ...
• Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity

  Dipankar Dutta, et al., bioRxiv - Bioengineering 2023

  Quote: ... the conditioned media was stored at -80° C and the interleukin-6 (IL-6) content was quantified via enzyme-linked immunosorbent assay (R&D Systems, DY406) following the manufacturer’s protocol.
• Three-Dimensional Bioconjugated Liquid-Like Solid (LLS) Enhance Characterization of Solid Tumor - Chimeric Antigen Receptor T cell interactions

  Duy T. Nguyen, et al., bioRxiv - Bioengineering 2023

  Quote: ... including inflammatory cytokines and chemokines using mouse IFNg DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA). In addition ...
• Cytokine-Mediated Degradation of the Transcription Factor ERG Impacts the Pulmonary Vascular Response to Systemic Inflammatory Challenge

  Christopher M. Schafer, et al., bioRxiv - Cell Biology 2023

  Quote: ... TNFα (R&D Systems: #410-MT), IL-1α (Abcam ...
• Cytokine-Mediated Degradation of the Transcription Factor ERG Impacts the Pulmonary Vascular Response to Systemic Inflammatory Challenge

  Christopher M. Schafer, et al., bioRxiv - Cell Biology 2023

  Quote: ... or IL-18 (R&D Systems: #9139-IL) were performed via the retro-orbital sinus ...
• A Potent and Selective CDKL5/GSK3 Chemical Probe is Neuroprotective

  Han Wee Ong, et al., bioRxiv - Cell Biology 2023

  Quote: ... IGF-1 (R&D Systems), and CNTF (R&D Systems).38 Next ...
• Cystin is required for maintaining fibrocystin (FPC) levels and safeguarding proteome integrity in mouse renal epithelial cells A mechanistic connection between the kidney defects in cpk mice and human ARPKD

  Yiming Zhang, et al., bioRxiv - Cell Biology 2023

  Quote: ... p62/SQSTM1 (mouse mAb, R&D Systems; MAB8028; 1:1000), pan-polyubiquitin (rabbit pAb ...