Labshake search
Citations for R&D Systems :
2601 - 2650 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2020Quote: Fecal lipocalin-2 levels in distal colon fecal contents were analyzed using Mouse Lipocalin-2 DuoSet ELISA (R&D Systems, DY1857) according to manufacturer provided instructions ...
-
bioRxiv - Microbiology 2019Quote: ... and lipocalin-2 (Lcn-2) protein was assayed in Mouse Lipocalin-2/NGAL DuoSet ELISA (R&D Systems, Minneapolis, MN, USA). At the end of DSS treatment ...
-
bioRxiv - Microbiology 2020Quote: ... Murine KC and JE concentrations were determined in the lung homogenate supernatants by DuoSet® ELISA Development Systems (R&D Systems), according to manufacturer instructions.
-
bioRxiv - Cell Biology 2019Quote: ... Luminex® Multiplex and ELISA assays for the detection of chemokines and cytokines were purchased from R&D Systems (Minneapolis, MN).
-
bioRxiv - Immunology 2021Quote: ... Protein lysates from colon tissue (normalized to 3.5 mg/mL) were assayed in duplicate using the Mouse IL-17 DuoSet ELISA (R&D Systems) according to manufacturer’s instructions.
-
bioRxiv - Immunology 2021Quote: IL-10 and TNFα in serum and CM-MSC were quantified using mouse Quantikine ELISA IL-10 immunoassay (R&D Systems) and TNFα ELISA high sensitivity (eBioscience ...
-
bioRxiv - Microbiology 2023Quote: ... Supernatants are collected and frozen at -20°C for cytokine assays performed by ELISA according to manufacturer’s instructions (DuoSet, R&D Systems).
-
bioRxiv - Immunology 2023Quote: ... supernatant from ex vivo culture and murine BAL fluid following flushing of airway 3X with 600uL of sterile PBS were used to quantify IL-1β levels by ELISA as per manufacturer’s instructions (R&D systems). Samples were first concentrated 5X (BAL ...
-
bioRxiv - Immunology 2023Quote: ... Just prior to analysis plasma samples were thawed and then assayed for numerous analytes (using dilutions optimized in prior studies) according to the manufacturer’s guidelines: adiponectin using the adiponectin/Acrp30 ELISA (DY1065, R&D System, lower limit of detection [LLoD] 15.6pg/ml) ...
-
bioRxiv - Physiology 2024Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...
-
bioRxiv - Bioengineering 2022Quote: ... Supernatant was collected and cytokines were quantified using mouse cytokine & chemokine convenience 6-Plex kit (IL-2, IL-4, IL-5, IL-10, IFN-γ and TNF-α; # LXSAMSM-06, R&D systems) using Luminex xMAP according to manufacturers protocols.
-
bioRxiv - Neuroscience 2020Quote: ... L-glutamine and 10 ng/ml of carrier-free murine recombinant granulocyte and macrophage colony-stimulating factor (GM-CSF, 415-ML, R&D systems, Minneapolis, MN). Cell suspension from one brain was plated in a T75-cell culture flask coated with poly-D-lysine (P7280 ...
-
bioRxiv - Immunology 2021Quote: ... the bone marrow cells were culture in 6-well plates at density of 3×106 cells/mL in 3mL DC medium in the presence of mouse granulocyte macrophage-colony stimulating factor (GM-CSF; 1000 U/mL; R&D Systems, Minneapolis, MN) and interleukin 4 (IL-4 ...
-
bioRxiv - Neuroscience 2022Quote: ... were grown in their undifferentiated form on 20ug/mL Cultrex reduced growth factor basement membrane (catalog number 3433, R&D Systems, Minneapolis, Minnesota, USA) in DMEM/F12 (catalog number 11320033 ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 3 uM CHIR, 300 nM LDN193189 (LDN, Axon, Scottsdale, AZ) and 100 ng/mL epidermal growth factor (EGF, R&D systems, Minneapolis, MN) in Advanced DMEM/F12 (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by overnight incubation with the primary antibody for platelet-derived growth factor receptor alpha (PDGFRa) (ref. AF1062-SP, R&D systems, 1/200), OLIG2 (ref ...
-
bioRxiv - Neuroscience 2021Quote: The clearance assay in human neurons was performed as described for primary pure murine neurons except that human IFN-γ (R&D Systems, Cat # 285-MI-100) was used for pre-stimulation.
-
bioRxiv - Immunology 2024Quote: ... Hematopoietic progenitor cells (HPC) were collected using Lineage Cell Depletion kit (Miltenyi Biotec, Bergisch Gladbach, Germany or R&D Systems, Minneapolis, MN). Then HPC were cultured in complete RPMI 1640 media with 10 ng/mL M-CSF (PeproTech ...
-
bioRxiv - Neuroscience 2023Quote: ... for 5 min each time and subsequently incubated with blocking buffer (5% goat serum [R&D Systems, Minneapolis ...
-
bioRxiv - Cell Biology 2020Quote: ... Bone marrow cells were plated in four replicates in 24-well plates at a density of 5 × 104 cells/well with 10 ng/ml of M-CSF (R&D Systems). Three days later ...
-
bioRxiv - Immunology 2022Quote: ... ex vivo primary B cells were first cultured for 12 h in complete media supplemented with 5 ng/ml BAFF (R&D Systems) and 2.5 µg/ml Escherichia coli O111:B4 LPS (Millipore Sigma ...
-
bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cell lines were incubated with 5 µL of 25 µg/mL anti-hCD3ε antibody (PE Conjugated Mouse IgG1, R&D System) or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
-
bioRxiv - Immunology 2020Quote: ... were generated by incubation of bone marrow cells for 5 days in the presence of 10 ng/ml of recombinant murine GM-CSF and IL-4 (R&D Systems), as described previously [40] ...
-
bioRxiv - Molecular Biology 2022Quote: ... human fibroblasts with either circGLIS3 depletion or overexpression or 3T3 cells with circGlis3 knockdown that had been transfected for 24h were stimulated with 5 ng/ml TGF-β1 (R&D Systems) for 24 hours ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were incubated with 5 µL (1:200) donkey anti-sheep IgG APC-conjugated antibody (R&D Systems, Cat. No. F0127) for 30 minutes on ice ...
-
bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
-
bioRxiv - Immunology 2023Quote: 5×106 SiLP cells were incubated in 250ul of complete media containing 20ng/ml IL-1β and IL-23 (R&D Systems) for 2 hours at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... naïve B cells or CH12F3 cells were treated with 10ng/ml F(ab’)2 Fragment Goat Anti-Mouse IgM or 10ng/ml mIL-5 (R&D Systems) for 5 or 10 min and then collected for Western blotting or RNA extraction.
-
bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice for 20 min ...
-
bioRxiv - Cell Biology 2024Quote: ... Matrigel domes were carefully scraped off with a cut open P1000 pipet tip and transferred to 5 ml cell recovery solution (R&D systems) on ice ...
-
bioRxiv - Immunology 2021Quote: ... Cell lines were tested for mycoplasma using the MycoProbe Mycoplasma Detection kit (R&D Systems).
-
bioRxiv - Developmental Biology 2022Quote: ... Cells were screened for mycoplasma contamination using the MycoProbe Mycoplasma Detection Kit (R&D Systems), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: Apoptosis of tumor cells was determined with an Annexin-FITC staining kit (R&D Systems) as per the manufacturer’s instructions and analyzed by flow cytometry ...
-
MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Next day the plates were washed 3 times and the color development was performed using Duoset ELISA Development System (DY999, R&D Systems) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The ELISA was performed according to the published protocol [43] using these antibodies: anti-TrkB goat 1:1000 (#AF1494, R&D systems), anti-SorCS2 rabbit 1:2000 (#bs-11963R BIOSS) ...
-
bioRxiv - Immunology 2021Quote: ... Then the concentrations of IFNγ in the supernatant were determined by enzyme-linked immunosorbent assay (ELISA) following the manufacturer’s instructions (R&D systems, Inc.).
-
bioRxiv - Microbiology 2021Quote: ... IL-1β (cat No. DY401) and IL-12 p70 (cat No. DY419) by using the mouse DuoSet ELISA system (R&D Systems). In addition ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: All samples were assessed by direct-sandwich enzyme-linked immunosorbent assay (ELISA) under the manufacturer’s (R&D System Europe Ltd, Abingdon, UK) according to the National Institute for Biological Standards and Controls (Blanche Lane ...
-
bioRxiv - Cell Biology 2021Quote: ... or in the lavage or serum from mice after MSUc injection in the air pouch were measured by ELISA (R&D Systems) following manufacturer’s specifications.
-
bioRxiv - Bioengineering 2022Quote: The amount of osteoprotegerin (OPG) released from hMSC-seeded scaffolds was quantified by an OPG ELISA (DY805, R&D Systems, Minnesota, USA). Media was collected every 3 days for 14 days and was pooled and assayed at specific timepoints ...
-
bioRxiv - Microbiology 2020Quote: ... The concentration of LCN-2 in the supernatant was measured by using a DuoSet Mouse Lipocalin-2/NGAL ELISA (R&D Systems) kit ...
-
bioRxiv - Immunology 2020Quote: TGFβ-1 concentration in supernatant from 4day ILC1 co-cultures and SIO only controls was measured using the Mouse TGFβ-1 DuoSet ELISA (R&D Systems) with modified manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: Culture supernatants were diluted 20-fold with PBS + 1% BSA + 0.2% Triton X-100 then analyzed for HIV-p24 protein using the HIV-1 Gag p24 DuoSet ELISA Development System (R&D Systems) with the R&D TMB substrate reagent pack for detection ...
-
bioRxiv - Microbiology 2021Quote: ... Cytokines and chemokines were assayed from organ homogenates using the pro-inflammatory focused 32-plex (Eve Technologies, Calgary, CA) or IFNγ DuoSet ELISA (R&D Systems) according to manufacturer protocol ...
-
bioRxiv - Immunology 2021Quote: ... The supernatants were collected 72 hours post-stimulation and the levels of IFNγ and IL-10 determined by and ELISA (R&D Systems). IFNγ production was also assessed by ELISPOT ...
-
bioRxiv - Immunology 2021Quote: ... supernatants were harvested to evaluate hIL-8 secretion by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (R&D Systems, U.S.A.). Cell protein concentrations were determined using the BCA Protein Assay kit (Thermofisher Scientific ...
-
bioRxiv - Immunology 2020Quote: IL-27(p28) in BALF was measured with a mouse IL-27 p28/IL-30 DuoSet ELISA (R&D Systems, Minneapolis, MN), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... the clones were assayed for IFN-γ secretion in a peptide-specific manner following their coincubation with T2 cells pulsed with MART-126–35 or gp100209-217 [both commercially synthesized and purified (>95%) by reverse-phase HPLC by Biomer Technology] by ELISA (R&D Systems). The MART-126– 35 or gp100209-217–reactive clones were further expanded in a second-round exposure to anti-CD3 (30 ng/mL) ...
-
bioRxiv - Immunology 2020Quote: ... IL-8 and TNF from SP140 siRNA or scrambled-treated M1 macrophages (LPS-stimulated or unstimulated) were determined by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA; R&D systems) according to manufacturer’s protocol.
-
bioRxiv - Immunology 2021Quote: ... All sera were assessed by direct-sandwich enzyme-linked immunosorbent assay (ELISA) under the manufacturer’s (R&D System Europe Ltd, Abingdon, UK) protocol ...