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Citations for R&D Systems :
2451 - 2500 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Cell cultures were subsequently supplemented every two days with 1.5% CMG and 5 ng/mL of RANKL (R&D Systems) to induce osteoclastogenesis.
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed with PBS without Ca2+ and Mg2+and incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+ or with monoclonal rabbit anti-Human ACE2 (cat ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Genomics 2020Quote: ... IL-13 and IFN-γ in the supernatant (Quantikine ELISA D5000B, D1300B and DIF-50, R&D Systems, Minneapolis, MN). Specifically ...
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bioRxiv - Immunology 2019Quote: ... in which plasma levels of soluble CD14 (sCD14) were determined by ELISA-sCD14 Quantikine assay (R&D Systems Minneapolis, MN) according to the manufacturer’s protocol and surface expression of combined HLA-DR and CD38 on CD4+ and CD8+ T cells was analyzed by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Neuroscience 2020Quote: ... crassiceps excretory/secretory (E/S) product using commercially available ELISA reagents according to the manufacturer’s instructions (R&D Systems, Germany). 96 well plates were coated with 50 μl antibody solution (IL-1β ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were retained and secreted chemokines were measured by ELISA (IP-10, R&D Systems; I-TAC, R&D Systems) and/or Luminex (Milliplex Human 38-plex).
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bioRxiv - Biochemistry 2021Quote: ... TNFα and IL-6 in supernatants were measured using ELISAs (Mouse TNFα; Mouse CCL5; Mouse IL-6, R&D Systems) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2022Quote: ... the conditioned medium was collected and TNF-α was quantified by enzyme-linked immunosorbent (ELISA) assay from R&D Systems according to the instructions of the manufacturer.
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bioRxiv - Immunology 2022Quote: ... PTX3 and P-selectin were determined by enzyme-linked immunosorbent assay (DuoSet ELISA, R&D Systems and Cloud-Clone corp) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... culture supernatants were analyzed for antibody production as well as IL-2 and IFNγ secretion by ELISA (R&D Systems). In CD4 blocking expriments ...
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bioRxiv - Physiology 2020Quote: ... IL-33 in colonic mucosa of distal colon was analyzed using Mouse IL-33 DuoSet ELISA (R&D Systems, DY3626) according to manufacturer instructions.
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bioRxiv - Cell Biology 2019Quote: Cytokine (IL6 and CXCL8) levels in tissue culture supernatants were determined by ELISA assay (DY206 and DY208; R&D Systems). All assays were performed according to the manufacturer’s instructions and read on a CLARIOstar microplate reader (BMG Labtech) ...
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bioRxiv - Microbiology 2022Quote: ... and MIP-2 (CXCL2) concentrations were evaluated in the lung homogenate supernatants by DuoSet ELISA Development Systems (R&D Systems), according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Concentration of PCSK9 in the plasma and tissue samples were assessed by ELISA (Quantikine MPC900, R&D Systems, Minneapolis, MN). Protein extracts were obtained after lysis of tissues in 350-μL Tris HCl buffer pH=9.5 (10 mM ...
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bioRxiv - Neuroscience 2024Quote: ELISAs were used to quantify CTSB and CTSS levels in CSF (both R&D DuoSets, R&D Systems, Minneapolis, USA). CSF samples were diluted 20x with reagent diluent prior to ELISA analysis ...
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bioRxiv - Bioengineering 2024Quote: ... The DuoSet ELISA systems were used according to the manufacturer’s instructions (DY201, DY206, DY210, DY1607, DY217B, R&D Systems, respectively). Absorbance values were measured on Infinite® 200 Pro plate reader (Tecan ...
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bioRxiv - Pathology 2023Quote: Levels of active TGF-β1 in triceps were quantified using Quantikine ELISA mouse TGF-β1 immunoassay (R&D Systems, MB100B) according to the manufacturer’s recommendations and as previously described24 ...
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bioRxiv - Immunology 2023Quote: Serum IL-6R levels were measured using an immunoassay method validated at QPS (Quantikine ELISA; R&D Systems Minneapolis, MN). Serum IL-6 and CRP levels were measured using in vitro diagnostic methods validated at PPD (Roche Cobas ...
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bioRxiv - Microbiology 2023Quote: The levels of secreted IFN-β were measured by an IFN-β enzyme-linked immunosorbent assay (ELISA) (R&D Systems) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media was collected from supernatant at first passage and assayed with the Mouse VEGF DuoSet ELISA (R&D Systems) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were collected 24 h later and analyzed for IL-17A by DuoSet ELISA (R&D Systems, Minneapolis, USA). Concentrations of IL-17A in the used CM was 764 pg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... IFNγ and IL-2 production were measured using a quantitative ELISA per the manufacturer’s instructions (R&D Systems, Minneapolis, MN).
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bioRxiv - Pathology 2023Quote: ... The supernatant was analyzed using the mouse Lipocalin-2 enzyme-linked immunosorbent assay (ELISA) Duoset (DY1857-05, R&D Systems) according to the manufacturer’s instruction in serial dilutions.
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bioRxiv - Immunology 2021Quote: ... 109-006-127), 250ng/ml human recombinant MEGACD40L (Enzo, ALX-522-110) and 20ng/ml Recombinant human IL-4 (R&D system, 204-IL-010). Cells were collected at specific time points for analysis.
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bioRxiv - Cell Biology 2022Quote: ... Cells were grown in a humidified incubator at 37∘C and 5% CO2 and tested mycoplasma negative (MycoProbe® Mycoplasma Detection Kit, R&D systems). HEK293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... Spain) and supplemented with each one of the following combinations of growth factors: (i) 100 ng/ml Act A (R&D Systems, UK), (ii ...
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bioRxiv - Cancer Biology 2022Quote: ... culture medium was supplemented with 0.1 to 0.5 % BME (Cultrex Reduced Growth Factor Basement Membrane Extract, type 2, Pathclear, R&D Systems, 3533-005-02). We observed batch-to-batch variations of this product ...
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bioRxiv - Molecular Biology 2022Quote: ... from C57BL/6 mice were differentiated from proliferating mouse bone marrow progenitors through induction with 20 ng/ml granulocyte macrophage colony stimulating factor (GM-CSF) (R&D Systems, USA) over 6–9 days as described [35] ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived-dendritic cells (moDC) were obtained by incubation of monocytes with 1 ng/mL of granulocytes-macrophages colony-stimulating factor (GM-CSF, R&D Systems, France) and Interleukin-4 (R&D Systems ...
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bioRxiv - Microbiology 2023Quote: ... Harvested cells were cultured in RPIM 1640 medium containing 20% porcine serum and 10 ng/mL recombinant porcine granulocyte-macrophage colony-stimulating factor (GM-CSF, R&D Systems, UK) to differentiate mature bone marrow-derived macrophage (BMDMs ...
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bioRxiv - Neuroscience 2023Quote: ... ectodermal paired box 6 and SRY-box transcription factor 2 (PAX6/SOX2, Biolegend, San Diego, CA, USA / R&D Systems, Minneapolis, MN, USA), and forkhead box protein A2 (FOXA2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were treated with assay medium supplemented with 10 ng/ml transforming growth factor-beta 1 (hereafter referred to as TGF-β, 240-B-002, R&D Systems) for 24 h or 72 h (Peinado et al. ...
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bioRxiv - Cell Biology 2024Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
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bioRxiv - Bioengineering 2019Quote: ... HUVECs were encapsulated (5·106 cells/mL) in situ within hydrogels loaded with 200 pmol/mL VEGF-165 (R&D Systems) or within non-loaded hydrogels (without GF) ...
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bioRxiv - Biochemistry 2020Quote: ... Jurkat E6.1 cells were incubated with 5 µL of 25 µg/mL anti-hCD3ε (PE Conjugated Mouse IgG1, R&D System) antibody or 2 µL of 200 µg/mL isotype control (PE Mouse IgG1 ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed three times in ice-cold DPBS buffer supplemented with 0.5% BSA and aliquoted to a density of 5× 106 cells/ml in Flow Cytometry Staining Buffer (FC001, R&D Systems). Next ...
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bioRxiv - Developmental Biology 2020Quote: ... and Anti-rat HRP-DAB Cell & Tissue Staining Kit (CTS017) from R&D Systems were used.
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bioRxiv - Systems Biology 2022Quote: ... avidin and biotin blocking reagents (HRP-DAB Cell &Tissue Staining Kit, R&D Systems) and washing in TBST in between each blocking step ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were verified mycoplasma negative using the MycoProbe Mycoplasma Detection Kit (R&D Systems). For serum-starvation experiments ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were isolated and stained with anti- human CD3 and CD8a antibodies (surface staining) followed by intracellular staining with anti- human IFNγ (R&D Systems, Cat. No. IC285F-100) antibody ...
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bioRxiv - Developmental Biology 2021Quote: ... 5 nM BMP4 (R&D systems), 1 μg/mL doxycycline (Sigma-Aldrich)) ...
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bioRxiv - Neuroscience 2020Quote: ... 100ng/ml human FLT-3 ligand (R&D Systems; 308-FKE), 20ng/ml human IL-3 (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... and Recombinant Human CXCL1/GROα Protein (R&D Systems, 275-GR).
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bioRxiv - Cancer Biology 2022Quote: ... Recombinant Human CCL20/MIP-3α Protein (R&D Systems, 360-MP), IL-8 Monocyte Recombinant Human Protein (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: ... or the control human Fc dimer (R&D Systems, 100-HG) was added to slice cultures at the indicated concentrations for the last 48 hours of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.9 μg/μl of human ubiquitin (R&D systems U-100H) or buffer only (10 mM Hepes ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human SUMO1-AMC and SUMO2-AMC proteins (R&D Systems) were diluted in parallel to 500 nM in Assay buffer (Tris HCl pH 8.0 50 mM ...