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Citations for R&D Systems :
2751 - 2800 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... 9 ng/ml Recombinant Human/Mouse/rat Activin A (R&D Systems) and 5 ng/ml human FGF-2 (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2023Quote: ... sheep anti-human Nephrin (1:40, AF4269-SP, R&D Systems, RRID:AB_2154851), goat anti-human RET (1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... 50 μM of human recombinant untagged ubiquitin (R&D SYSTEMS, U-100H) and specific human recombinant DUBs were added at the same concentration as for the proteomic experiments (800 nM) ...
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bioRxiv - Cell Biology 2023Quote: ... and recombinant human FGF basic146 aa (10 ng/ml, R&D Systems) for another 2 weeks as previously described(60).
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bioRxiv - Immunology 2023Quote: ... and recombinant human IL-2 (rhIL2,50 U/mL; R&D Systems/CellGenix)) with or without 1% penicillin/streptomycin/l-glutamine (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... and 100 ng/ml human recombinant APRIL/TNFSF-13 (R&D systems). Human healthy donor peripheral blood mononuclear cells (PBMC ...
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bioRxiv - Immunology 2024Quote: ... recombinant human TNFα (10 ng/ml) (10291-TA-020, R&D Systems), poly I:C (1µg / ml ...
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bioRxiv - Biophysics 2024Quote: ... and/or human Wnt5a (R&D Systems Inc., 645-WN-010/CF) were added to cell culture medium during the microscopy experiments ...
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bioRxiv - Cancer Biology 2024Quote: ... Recombinant human IFN-γ was purchased from R&D Systems (Minneapolis, MN).
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bioRxiv - Immunology 2021Quote: ... and SDF-1a), and other factors (PD-L1, PDGF-BB, S100B, GM-CSF, and VEGF-A) validated for NHP (R&D Systems, Minneapolis, MN, USA). Standard curves were generated using 5-parameter logistic regression using the xPONENT™ software provided with the MAGPIX instrument (Luminex ...
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bioRxiv - Immunology 2021Quote: ... 50 ng/mL of recombinant human GM-CSF (Sanofi-aventis Cat# NAC2004–5843-01) and 2.5 ng/mL of tumor necrosis factor (TNF)-α (25 ng/mL; R&D Systems Cat# 210-TA). Next ...
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bioRxiv - Cell Biology 2022Quote: ... hMEC 3D acini were resuspended in 10 mg/ml Cultrex Reduced Growth Factor Basement Membrane Extract (BME) Type 2 (R&D Systems, #3532-005-02) and 25 μl BME domes were plated in each well of µ-Slides 8 (Ibidi) ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Biophysics 2023Quote: ... A “type-1” like cytokine cocktail consisted of 10 ng/ml of recombinant mouse tumor necrosis factor-α (TNF-α, R&D Systems, Minneapolis, MN), 5 ng/ml of recombinant mouse interleukin-1β (IL-1β ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% FBS and 1% penicillin/streptomycin with macrophage-colony stimulating factor (M-CSF, 30 ng/mL; 416-ML, R&D systems, Minneapolis, MN, USA) for 4 days to obtain BMMs ...
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bioRxiv - Bioengineering 2023Quote: RFP-MDA-MD-231 and GFP-HUVEC at 3 million/mL and 8 million/mL in precursor solution were photopatterned and perfused using the peristaltic pump previously mentioned at 0.2 rpm/mL for five days before tumor necrosis factor-alpha (TNF-α) (R&D Systems, Minneapolis, MN, USA) and Vascular endothelial growth factor (VEGF ...
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bioRxiv - Immunology 2024Quote: ... mice with 20% L929-conditioned medium (LCCM) in DMEM and 20ng/mL recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems, Minneapolis, MN, USA) in RPMI for BMDM and BMDC ...
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bioRxiv - Cancer Biology 2024Quote: Transparent PET membrane culture inserts of 24-well plate (Falcon, 353097) were coated with Cultrex Reduced Growth Factor Basement Membrane Extract (BME) (R&D Systems, 3533-010-02) at 50μg per membrane (200μl of 0.25mg/ml BME stock per membrane ...
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bioRxiv - Cancer Biology 2024Quote: Cell culture plates for cell invasion assays were prepared by adding 150 μL of 1 mg/mL Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, 3433-005-R1) to a 24-well cell culture insert with an 8 μm pore size (Falcon ...
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bioRxiv - Cancer Biology 2021Quote: ... IL15 (5 ng/ml; R&D Systems) and PHA-L (1 μg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml TGF (R&D Systems) and 5 ng/ml of mouse recombinant IL-4 for 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... 5 ng/ml bFGF (R&D Systems) and 1 µmol Thiazovivin (Milipore) ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml TGFβ (R&D Systems) and 5 ng/ml of mouse recombinant IL-4 for 48 h ...
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bioRxiv - Immunology 2022Quote: ... and recombinant IL-5 (R&D systems) at an amount of 50 ng/sample were incubated overnight in kit specific reaction buffer with 1μg/ml rSmCI-1 ...
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bioRxiv - Pathology 2022Quote: ... rIFN-γ (5 µg, R&D Systems), or rIL-13 (5 µg ...
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bioRxiv - Developmental Biology 2023Quote: ... 5 ng/ml BMP4 (R&D Systems), 2 ng/ml TGFB1 (R&D Systems ...
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bioRxiv - Cell Biology 2022Quote: ... eMSC were treated with N-[N-(3,5-difluorophenacetyl-l -alanyl)]-(S)-phenylglycine t-butyl ester (1.25 µM, DAPT, R&D Systems) and cultured in GM for 7 days.
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bioRxiv - Cancer Biology 2021Quote: ... The cells were resuspended in 100 µl total volume and stained with 5 µl anti-His-PE (#IC050P, R&D Systems, Minneapolis, MN) or anti-CD19 antibody FMC63-PE (#MAB1794 ...
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bioRxiv - Bioengineering 2020Quote: Cytokine and chemokine levels were measured in the basal outflow of mouse intestine chips using a custom MSD U-plex Assay (Meso Scale Diognostics) and the mouse CXCL1/KC DuoSet ELISA (R&D Systems DY453) according to the manufacturers’ instructions.
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bioRxiv - Microbiology 2021Quote: Human IL-29/IL-28B (IFN-lambda 1/3) concentration in SARS-CoV-2 infected epithelium basal media was quantified using ELISA technics following manufacturer’s recommendations (R&D systems, Minneapolis, USA). 100 μl of media was used for each point.
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bioRxiv - Cell Biology 2021Quote: ... IL-8 and TNF-α were quantified in the supernatants from uninfected and SARS-CoV-2-infected hiPSC-CMs by ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... progranulin concentrations were determined in duplicate using 10–15 μl of lysates per well (typically 8–20 μg of total protein per well) using a sandwich ELISA assay (R&D Systems, DPGRN0). For experiments analyzing secreted progranulin ...
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bioRxiv - Immunology 2019Quote: The p24 ELISA with supernatant from infected and non-infected LPMC cultures was performed according to the manufacturer’s protocol (R&D Systems, Minneapolis, USA). Absorbance was measured using the Spark® 10M multimode microplate reader (Tecan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... in BALF were quantified by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s protocol (DY406, DY410 and DY401 from R&D systems; Minnesota, USA). The UV absorbance at 450 nm and 570 nm were measured for the primary signal and for plate correction ...
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bioRxiv - Immunology 2021Quote: ... and Ccl19/Ccl21 levels were determined by indirect sandwich of enzyme-linked immunosorbent assay (ELISA) following manufacturer’s instructions (R&D Systems, Minneapolis, MN). Multi-analytic profiling beads using Milliplex® Multiplex mouse cytokine/chemokine panel I kit (EMD Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... IL-6 was measured by ELISA (enzyme-linked immunosorbent assay) with the obtained supernatant and analyzed according to the manufacturer’s protocol (R&D Systems, Minnesota, USA).
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bioRxiv - Microbiology 2023Quote: ... Murine IP-10 production was measured from 100 μL of the supernatant from the BMDMs using Mouse CXCL10/IP-10/CRG-2 Duo Set ELISA (R&D systems, #Dy466) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... The concentrations of IL-6 and IL-8 were measured in triplicate with the DuoSet ELISA Development System (R&D Systems, Minneapolis, MN) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Doses were previously optimized and mice were intravenously injected with 5×1010 genome copies of both Control- and TSLP-AAV for 14 days with serum TSLP levels confirmed using a murine specific ELISA (R&D Systems MTLP00)4.
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bioRxiv - Cell Biology 2023Quote: ... plasma and tissues of interest were collected at different post-infection times and assessed by ELISA using commercially available antibodies and according to the procedures supplied by the manufacturer (R&D Systems, Minneapolis). All samples were stored at -20°C until processing ...
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bioRxiv - Cancer Biology 2023Quote: ... POSTN protein expression in the medium was confirmed using an ELISA assay with an anti-POSTN polyclonal antibody (R&D Systems, Cat # AF3548). 3T3-CTL cells were used to generate “control” conditioned medium (CMCTL) ...
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bioRxiv - Immunology 2024Quote: ... The supernatant was collected at indicated times and protein concentrations were determined by ELISA following the manufacturer’s instructions for IL-6 (R&D systems, DY406-05), TNFα (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... nitrite concentrations in cell culture supernatant were determined via Griess reaction assay kit (R&D Systems).
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bioRxiv - Cancer Biology 2022Quote: ... Slides were processed with a commercial DAB cell and tissue staining kit (#CTS019, R&D Systems). Primary antibody immunostaining was optimised for polyclonal rabbit anti-human peroxidasin (#abx101905 ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed by lysis buffer provided in the kit (R&D Systems, #895943). The supernatant was collected after centrifugation at 14,000xg for 5 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: Crypts isolated from mouse intestine were seeded at a density of 250 crypts per 25 µL Matrigel (Cultrex reduced growth factor basement membrane extract, type R1, R&D Systems, Catalog #3433-010-R1) in 48 well plates ...
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bioRxiv - Physiology 2024Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
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bioRxiv - Bioengineering 2024Quote: ... Organoid basal growth media was supplemented with epidermal growth factor (EGF) (100 ng/mL, 10 ng/mL, 1 ng/mL R&D Systems Cat#236-EG-01M) or Epiregulin (EREG ...
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bioRxiv - Cancer Biology 2021Quote: ... and human recombinant bFGF and EGF (25 ng/ml each; R&D Systems), hereafter ...
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bioRxiv - Genetics 2021Quote: ... Stimulation was performed using 10 ng/ml recombinant human TGFβ1 (R&D system). C646 dissolved in DMSO was treated at a dose of 20 μM for 24 hours of pretreatment before TGFβ1 stimulation ...