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Citations for R&D Systems :
2301 - 2350 of 4407 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Plasma levels of leptin and insulin were assessed using ELISAs used according to the manufacturer’s instructions (R&D Systems, #MOB00B and Crystal Chem ...
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bioRxiv - Cancer Biology 2020Quote: ... ELISA’s were performed using 50 μl of media per sample and following the manufacturer’s protocol (R&D systems, DTFF30). After removal of media ...
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bioRxiv - Cell Biology 2021Quote: ... and total MMP7 were quantified from device effluents using the DuoSet® line of ELISAs from R&D Systems according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... supernatants were collected and IL-2 concentration was determined using the Mouse DuoSet IL-2 ELISA (R&D Systems), following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: The concentration of secreted IFN-λ1 was measured by enzyme-linked immunosorbent assay (ELISA) (R&D Systems, DuoSet DY7246), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cytokines in lung homogenate and BALF were quantified using Duoset ELISAs according to the manufacturer’s instructions (R&D systems).
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bioRxiv - Immunology 2021Quote: ... IFNγ) level in the supernatant was determined after 48 and 72 h of stimulation using ELISA (R&D Systems).
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium supernatant was collected and subjected to enzyme-linked immunosorbent assay (ELISA) of secreted IL6 (R&D Systems) detected by absorbance at 450 nm and 570 nm on Molecular Devices SpectraMax M2 Microplate Reader ...
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bioRxiv - Synthetic Biology 2024Quote: ... CHO-derived rhBMP2 standard and streptavidin-HRP working solution from the hBMP2 DuoSet ELISA system (DY355, R&D systems) were used for all experiments ...
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bioRxiv - Microbiology 2024Quote: ... KC in mouse lungs was analyzed with the Mouse CXCL1/KC DuoSet ELISA (#DY453, R&D Systems, Minneapolis, MN). Cytokines secreted by primary CF-HBECs were analyzed by the MILLIPLEX MAP Human Cytokine/Chemokine 48-Plex cytokine assay (Millipore) ...
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bioRxiv - Physiology 2024Quote: Levels of IL-22 were measured by double-antibody sandwich enzyme-linked immunosorbent assays (ELISAs) (M2200; R&D Systems). Briefly ...
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bioRxiv - Immunology 2024Quote: ... Subsequently, supernatants were evaluated for cytokine concentrations (TNF, IL6 and IL1β) via ELISA Duoset (R&D Systems, Minneapolis, MN). Each sample was assayed in triplicate ...
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bioRxiv - Neuroscience 2022Quote: ... CXCL10 was measured in neuronal culture supernatant with Mouse CXCL10 DuoSet ELISA (R&D Systems DY466-05, Minneapolis MN) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and IL-1β present in the CM samples were quantified by ELISA using antibodies obtained from R&D Systems according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... SLPI and neutrophil elastase (NE) were measured using the Mouse SLPI DuoSet ELISA (CAS: DY1735-05, R&D Systems) and Mouse Neutrophil Elastase (ELA2 ...
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bioRxiv - Microbiology 2024Quote: ... Cytokine levels for IL-6 and MCP-1 were determined by ELISA according to manufacturer’s instructions (R&D Systems).
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bioRxiv - Developmental Biology 2019Quote: ... Both wild type and Adgrg6 KO ATDC5 cells were treated with 100ng/ml recombinant human IL-6 protein (rIL-6) (R&D System, 206-IL) for 2 hours before protein extraction.
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bioRxiv - Bioengineering 2021Quote: Conditioned media from each cell-line/passage (at the time of cell harvest) were collected and prepared for cytokine profiling using the Human Premixed Multi-Analyte Magnetic Luminex Assay (R&D Systems, Minnneapolis MN) (Supplemental Table 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were maintained in serum-starved media with or without 10ng/ml of recombinant human TGF-β1 (R&D Systems, cat 240-B/CF) for 48 hours and harvested ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 1 μg/mL of either human rPD-1 (hrPD-1-Fc; R&D Systems #1086-PD-050; Minneapolis, MN, USA) or mouse rPD-1-Fc (R&D Systems #1021-PD-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Experiments investigating EV-free AREG were undertaken by incubating recipient cells with 0.03 – 3000 ng/ml recombinant human Amphiregulin protein (R&D Systems #262-AR-100), for 30 minutes at 37°C before plating as described above.
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bioRxiv - Immunology 2019Quote: ... 5 ng/ml IL-7 and 5 ng/ml IL-15 (R&D Systems).
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Human MΦ were then polarized to a M1 activation state with 30 ng/mL human recombinant IFN-γ (R&D Systems, 285-IF-100) and LPS (100 ng/mL ...
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bioRxiv - Cell Biology 2024Quote: The following antibodies were used: monoclonal mouse anti-human CXCR4 (clone 44717) and phycoerythrin-conjugated human CXCR4 (clone 12G5; both from R&D Systems, Minneapolis, MN); purified mouse anti-human CD3 (clone HIT3a ...
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bioRxiv - Cancer Biology 2022Quote: ... incubated overnight at 4°C in the following primary antibodies: T-LCK (1:1000 R&D Systems), T-LCK (1:1000 Proteintech) ...
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bioRxiv - Immunology 2022Quote: ... sterile filtered and used in a Milliplex assay to measure cytokines and chemokines in each sample using a 5- and 25plex customized kit (Millipore and R&D Systems). Data were analyzed by calculating the average value of the biological duplicates and then the ratio of Mtb treated / control ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lysates were analyzed with the Phospho-RTK Array Kit (R&D Systems). Immunoblotting analyses was performed using phospho-Axl antibody (R&D systems ...
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bioRxiv - Immunology 2021Quote: ... Cells were assessed for apoptosis with Annexin V staining kit (R&D Systems) per manufacturer’s instructions and were analyzed immediately via flow cytometry.
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bioRxiv - Evolutionary Biology 2024Quote: ... Cell supernatants were tested for mycoplasma contamination by MycoProbe Kit (R&D Systems) or MycoStrip (Invi-voGen) ...
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bioRxiv - Neuroscience 2022Quote: ... The cells were cultured for 96 hours with MOG35-55 peptide (50 μg/mL) and Th17-polarizing factors as follows: recombinant murine (rm)IL-23 (8 ng/ml; R&D Systems), rmIL-1α (10 ng/ml ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in RPMI 1640 supplemented with 10% serum and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; R&D Systems) for 3 days ...
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bioRxiv - Immunology 2019Quote: ... from wild type and Irak1−/− mice were prepared by differentiation for 6 days in the same culture medium containing macrophage colony-stimulating factor (M-CSF; 60 ng/mL, R&D Systems). LPS was from Alexis Biochemicals (Salmonella minnesota R595 TLR grade ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were added to the culture plates at the density of 2.0 x 106 cells / dish in a final volume of 10 mL of complete RPMI-1640 supplemented with 20 ng/mL GM-CSF (granulocyte-macrophage colony stimulating factor; R&D Systems). After 3 days ...
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bioRxiv - Bioengineering 2021Quote: ... dickkopf-related protein-1 (Dkk-1) and transforming growth factor beta 1 (TGF-β1) were obtained from R&D systems (Minneapolis, MN) and performed on conditioned media from hMSCs grown on GelMA and OCM-GelMA microcarriers in RWV bioreactors.
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bioRxiv - Bioengineering 2021Quote: ... wells were washed and the growth factors remained bond to the heparin conjugates were detected by incubation with the corresponding antibody (R&D systems) followed by a peroxidase-labeled secondary antibody (Jackson ImmunoResearch) ...
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bioRxiv - Microbiology 2021Quote: ... gradient and treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) respectively for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... serum starved at 37°C in MV2 medium (containing no growth factors) 3 hrs before stimulation with VEGFA164 (50 ng/ml; R&D Systems), followed by fixation in 3% PFA for 3 min ...
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bioRxiv - Neuroscience 2019Quote: ... for 24h or with 10 ng/mL tumor necrosis factor alpha and 10ng/ml IL1-ß(R&D Systems; 401-ML-005) for 24h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Genetics 2020Quote: ... femurs and tibias were harvested and bone-marrow cells were obtained by flushing bones and differentiated for 7 days in DMEM media supplemented with 50 mg ml-1 recombinant macrophage-colony stimulating factor (M-CSF, R&D systems), 20% heat-inactivated FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Intestinal organoids were grown in base culture media (Advanced DMEM/F12 media, HEPES, GlutaMax, penicillin, and streptomycin) supplemented with growth factors (EGF, Noggin, R-spondin, R&D Systems), B27 (Life Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... and then treated with macrophage colony stimulator (M-CSF) or granulocyte-macrophage-stimulating factor (GM-CSF) and IL-4 (R&D system) for 7 days.
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bioRxiv - Neuroscience 2022Quote: ... either vehicle (PBS) or candidate factors were added at the following concentrations: BDNF (5ng/mL, R&D Systems, Cat# 248-BDB-005), GDNF (5ng/mL ...
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bioRxiv - Immunology 2022Quote: ... The differentiation to macrophages was initiated at day 0 by the addition of 20 ng/mL macrophage colony-stimulating factor (M-CSF, R&D Systems). Cells were cultured afterwards at 37°C and 5% CO2 for 7 days including the replacement of supplemented DMEM and replenishment of M-CSF at days 1 and 4 ...
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bioRxiv - Cell Biology 2024Quote: ... crypts per isolated from murine intestine and cultured in domes of Cultrex Pathclear Reduced Growth Factor Basement Membrane Extract (3533-005, R&D Systems), covered by ENR media comprised of Advanced DMEM/F12 (12634028 ...
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bioRxiv - Cell Biology 2024Quote: ... into a 96-well plate pre-coated with non-diluted Cultrex reduced growth factor base membrane extract (RGF-BME) (R&D Systems). The images of the network structure were captured by IncuCyte S3 real-time imager every 2 h at 4X magnification ...
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bioRxiv - Microbiology 2024Quote: ... and were differentiated into monocyte-derived macrophages (MDMs) by stimulation with 20 ng/mL macrophage colony-stimulating factor (M-CSF) (R&D Systems). MDMs were cultured in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Developmental Biology 2023Quote: ... embryonic NSC media was prepared as above without epidermal growth factor and with the addition of 50 ng/mL mouse bone morphogenetic protein 4 (R&D Systems, 5020-BP-010 ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from healthy human blood donors after preparing blood monocyte monolayers and culturing for 2 days in medium containing macrophage colony-stimulating factor (R&D Systems) as described previously (56 ...
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bioRxiv - Bioengineering 2024Quote: MDA-MB-231 spheroids were formed starting from a culture of 10,000 cells per spheroid mixed with their medium and a 2% v/v Cultrex UltiMatrix Reduced Growth Factor Basement Membrane Extract (BioTechne, R&D systems) solution in 96-well round-bottom plates (Corning Costar Ultra-Low Attachment Multiple Well Plate) ...