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Citations for R&D Systems :
2101 - 2150 of 2812 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Mouse tibialis anterior muscles were injected with 50 uL solution of 50,000 human FAPs resuspended in 50% glycerol in PBS with either 29ng/ul concentration of recombinant DLK1 resuspended in PBS (R&D Systems, #1144-PR-025) or with control (identical volume of PBS ...
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bioRxiv - Cancer Biology 2019Quote: ... lymph node tissue sections derived from mice bearing pancreatic tumors were incubated overnight at RT with the following primary antibodies: NKp46 (R&D systems AF2225, used at 1:25), Ki67 (Abcam ab15580 ...
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bioRxiv - Genomics 2023Quote: ... Primary antibodies (FUS 1:100 Santa Cruz Biotech sc-47711; ISL1 1:500 Abcam ab109517; OLIG2 1:100 R&D Systems AF2418; NFM 1:1000 Merck MAB1621) were added in 1% BSA and incubated at 4degC overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Media was then exchanged to DMEM containing 15 ng/mL recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF; R&D Systems # 215-GM-010/CF). PBMC Mϕ were allowed to differentiate for an additional 5 days and were returned to DMEM 10% FBS until stimulation.
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bioRxiv - Neuroscience 2019Quote: The secretion of cytokines in AD TREM2 neuronal cultures before and after stimulation with the Aß-S8C dimer was measured employing the Proteome Profiler Human Cytokine Array kit (R&D System, United States of America). The assay was performed following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... IP-10 and TGF-β levels were measured by enzyme-linked immunosorbent assay (ELISA) sandwich using human CXCL-10/IP-10 DuoSet ELISA (R&D Systems Inc, MN, USA) and human/mouse TGF beta 1 ELISA Ready-SET-Go! Kit (2nd Generation ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were pre-treated for 1 day with vehicle control (0.1% BSA in 1X PBS) or 400 ng/ml human recombinant IL-1RA (R&D Systems, Minneapolis, MN; 280-RA/CF) 0 in the DMEM/10% FBE growth medium ...
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bioRxiv - Neuroscience 2020Quote: ... while MABs differentiation into myotubes was initiated in the opposite compartments using MAB differentiation medium containing 2% horse serum (Cat N° 16050122) and 1% sodium pyruvate in DMEM/F12 supplemented with 0.01 μg/ml recombinant human agrin protein (R&D Systems, Cat N° 6624-AG-050). At day 21 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were incubated 2 h prior to infection and maintained throughout with either,1 μg/mL mouse monoclonal anti-human IL-10 antibody (R&D Systems. Ref: AF-217-NA), or equivalent concentrations of human IgG (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: The presence of angiogenic factors in BEC CM was evaluated trough a Proteome Profiler Human Angiogenesis Array kit (ARY007, R&D Systems Inc., Minneapolis, MN, USA). For this ...
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bioRxiv - Physiology 2020Quote: Serum samples were assayed for IL-6 concentration using commercially available human IL-6 enzyme linked immunosorbent assay kits (Quantikine®, R&D systems, Minneapolis, MN, USA) according to the manufacturer's instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 3 times and incubated with 80 µL per well of monoclonal mouse IgG2B human sRAGE or galectin-3 antibody (R&D Systems, Minneapolis, Minnesota, United States) at a concentration of 0.2 µg/ml for 30 minutes shaking at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Media supernatant was collected from the cells at the end of the experiment and IL-1β protein was analysed using an IL-1β Human Luminex® Discovery Assay kit (R&D Systems, LXSAHM; Abingdon, UK).
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bioRxiv - Neuroscience 2024Quote: ... explants were treated with 10 ng/mL human recombinant IL- 6 and soluble IL-6 receptor (IL-6-sIL-6R) (1:1; R&D Systems 206-IL-050; I5771) for 20 mins ...
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bioRxiv - Biochemistry 2024Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
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bioRxiv - Cell Biology 2024Quote: ... heat shock treated cells were lysed with 1× cell lysis buffer without 20 mM NEM and then incubated with 220 nM recombinant Human Sentrin-specific protease 1 (SENP1) catalytic domain (R&D Systems, Catalog no. E-700) at 37 °C for 20 min before immunoblotting analysis.
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bioRxiv - Cancer Biology 2024Quote: Cell viability of siRNA transfected cells seeded in 96 well-plates was quantified using the Resazurin cell viability assay (R&D Systems AR002). For apoptosis assays ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentration of active MMP9 on 200μg of total proteins lysate was quantified by using a Human Active MMP-9 Fluorokine E assay kit (F9M00; R&D Systems, Noyal Châtillon sur Seiche, France) according to the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2023Quote: ... Subsequently the cells were rested and expanded in kool aid complete media for 2 days supplemented with 100 U/mL recombinant Human IL-2 (R&D Systems Cat# 202-IL-010/CF). Subsequently ...
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bioRxiv - Physiology 2023Quote: ... The supernatants were diluted when necessary to be in the optimal linear optical range and analyzed using commercial kits for IFNγ (Human IFN-gamma DuoSet ELISA, R&D Systems, USA and Bio legend, UK), and IL-10 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... were washed and resuspended in PBS with 0.02% Tween-20 (PBS-T) and loaded with recombinant human IL13Rα2-Fc chimera (R&D Systems Cat#614INS; 2.1 µg per 3.9×107 beads) by incubation for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated in the same buffer for 1 h under light agitation with the recombinant murine chimera proteins containing human Fc domains and the extracellular domains of TREM2 (R&D systems, 1729-T2-050, 0.1 mg/mL) or CD14 (R&D systems ...
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bioRxiv - Microbiology 2023Quote: Catalytic activities of SpyCEP constructs were measured through detection of remaining intact CXCL8 or CXCL1 substrate following incubation with SpyCEP by ELISA (R&D Systems human CXCL8 and CXCL1 DuoSet ELISA) as per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Murine CD3 T cells were isolated using Mouse CD3+ T Cell Enrichment Columns (R&D Systems) per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... A beveled glass needle was pre-loaded with mineral oil and the recombinant human IL-1 receptor inhibitor (IL-1RA, R&D Systems, Cat # 280-RA, resuspended in 0.1% BSA), then positioned in the injecting rig at a 90° angle relative to the horizontal plane of the Petri dish and head mold ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic CD3+ T cells were isolated using CD3+ T cell enrichment columns (R&D Systems, MTCC25). T cells were cultured at 37C in RPMI-1640 (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... PBS was removed and 50 μl of cell lysis buffer (Cell Lysis Buffer 2, R&D Systems, # 895347) were added ...
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bioRxiv - Pathology 2023Quote: ... Cells were filtered through 40µm cell strainer with 4mL of RPMI + 5% FBS (R&D Systems, Minneapolis, MN), and the unfiltered tissues were further digested with 4mL of 0.05% Trypsin and incubated at 37°C for 10 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in B cell Excellerate medium with the CEllXVivo B cell expansion kit (R&D systems, UK). CD8+ T-cells (Cambridge Bioscience ...
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bioRxiv - Pathology 2024Quote: ... Cell surface protein expression was performed by labeling 2×105 cells for KDR-PE (R&D Systems, FAB357P) + PDGFRα-APC (Cell Signaling ...
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bioRxiv - Bioengineering 2020Quote: ... endothelial cell growth supplement (R&D Systems), L-glutamine and antibiotics (complete culture medium) ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were plated at 107 cells/well and differentiated with Dendritic Cell Differentiation Kit (cat# CDK004, R&D Systems) according to the manufactures instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended to 1 x 105 cells/mL and mixed with 1% low-melting agarose (R&D Systems) at a 1:10 ratio and plated on 2-well comet slides (R&D Systems) ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II (STEMCELL Technologies ...
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bioRxiv - Immunology 2022Quote: ... activated B cells were washed and cultured at 0.5.106 cells/ml with 50 U/ml IL-2 (R&D Systems), 5 ng/ml IL-4 (R&D Systems ...
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bioRxiv - Microbiology 2020Quote: ... PTPN14 (R&D Systems or Cell Signaling Technology), KRT1 (Santa Cruz) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with Wnt3a (R&D Systems), Noggin (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: mOSE cells (1×10^6 cells) were plated 24 hr prior to treatment with TGFB1 (10 ng/mL, R&D Systems). RNA was collected 7 days post-TGFB1 treatment (RNAeasy Kit ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were differentiated into macrophages and dendritic cells using 50 ng/ml GM-CSF (R&D Systems, https://www.rndsystems.com) + 50 ng/ml M-CSF (R&D Systems ...
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bioRxiv - Bioengineering 2021Quote: ... 12 million cells were then purified for CD90+ cells using a PE-conjugated antibody for anti-CD90 (R&D Systems, FAB2067P), the EasySep™ Human PE Positive Selection Kit II and EasySep™ Magnet.
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bioRxiv - Genomics 2021Quote: ... and cells were labelled with phycoerythrin (PE)-conjugated antibody against the cell surface marker FGFR3 (FAB766P, clone 136334, R&D systems). Spermatogonial cells were isolated using a FACSAria II cell sorter (BD bio- sciences ...
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bioRxiv - Molecular Biology 2022Quote: ... 96 wells-plate were used to plate the cells at 10,000 cells/well in RPMI +10% FBS supplemented or not by different concentrations of recombinant Tpo (R&D Systems). After 72 hours ...
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bioRxiv - Developmental Biology 2023Quote: Surface expression of ITFG1 on cells was examined by staining the cells with mouse anti-ITFG1 antibody (R&D Systems, MAB89001) at 4°C for 1 hour ...
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bioRxiv - Bioengineering 2023Quote: OCI-AML5 cells were cultured in 24-well plates (3×105 cells/well) in cRPMI media (FBS from R&D Systems; Minneapolis ...
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bioRxiv - Cancer Biology 2023Quote: ... CTSD for mouse cell lysates (#MAB1123-SP) and CTSA for mouse cell lysates (AF1029-SP) were bought from R&D Systems.
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bioRxiv - Cell Biology 2020Quote: ... Once the cells were adherent the cells undergoing differentiation were cultured in basal medium supplemented with 1% v/v Adipogenic Supplement (R&D Systems) while the control cells not undergoing differentiation were cultured in basal medium ...
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bioRxiv - Biochemistry 2022Quote: ... Cell identities were confirmed by satellite tandem repeat profiling and cells were tested to be free from mycoplasma (MycoProbe, R&D Systems). Cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2019Quote: ... Selected cytokine targets were measured in cell culture supernatants and cell lysates with commercially available Luminex® Multiplex Assays (R&D Systems) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Cells in ATP depletion were prepared by culturing the cells in a medium containing 100 μM CCCP (R&D systems, Minneapolis, MN) and 600 μM 2-deoxy-D-glucose (2DG ...
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bioRxiv - Immunology 2022Quote: ... T cells were stimulated for 48 hours at 1e6 cells per ml of media containing 500 U/ml IL-2 (R&D Systems), 5 ng/ml IL-7 (R&D Systems) ...