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Citations for R&D Systems :
2051 - 2100 of 2812 citations for Primary Human Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Cells were then seeded on the coated flasks and supplemented with 100 ng/ml of human PDGF-BB re-suspended in 0.1%BSA/4mM HCl/PBS (R&D Systems; 200-BB-050) daily for at least 7 days ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in PBS and incubated for 30 minutes at room temperature (RT) with the human α-PlexinD1 mouse monoclonal antibody (1:150 dilution) (R&D systems, MAB4160). Cells were washed twice with PBS and incubated for 30 minutes at RT with an α-Mouse IgG secondary goat antibody conjugated to the APC fluorochrome (1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... and Connective Tissue Growth Factor (CTGF) (DY9190-05) were measured using Human DuoSet Enzyme linked immunosorbent assay (ELISA) kits (R&D Systems, Abingdon, UK) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Five million PBMC were suspended in 40 ml R10 supplemented with 10 ng/ml recombinant human IL-2 (R&D Systems, 202-IL-050) together with 10 million K562-mbIL21-41BBL feeder cells irradiated with 100 Gy ...
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bioRxiv - Neuroscience 2022Quote: ... a mix of 10 μg/mL Laminin and 5 μg/mL of each SLRP protein (recombinant human CHAD (R&D Systems Cat#8218-CH), FMOD (R&D Systems Cat#9840-FM) ...
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bioRxiv - Physiology 2023Quote: ... 1nM) in VEH for 60 minutes prior to the addition of recombinant human TGF-β1 (5 ng/ml; R&D Systems, Minneapolis, MN, USA). At 6 ...
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bioRxiv - Cell Biology 2023Quote: ... Then lysates were utilized exactly according to the instructions in the Human Phospho-VEGFR3/Flt-4 DuoSet IC ELISA Kit (R&D systems, DYC2724-5).
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bioRxiv - Cancer Biology 2023Quote: ... TF1 cells were cultured in RPMI-1640 with 10% FBS and 1 ng/mL recombinant human granulocyte–macrophage colony-stimulating factor (rhGM-CSF, No. 215-GM, R&D Systems, Minneapolis, MN). 293T cells were cultured in DMEM media containing 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: ... proteins were extracted via lysis using a lysis buffer from the Proteome Profiler™ Human Apoptosis Array Kit (ARY009, R&D Systems, Minneapolis, MN) kit ...
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bioRxiv - Microbiology 2023Quote: The levels of CX3CL1 were determined according to manufacturer’s instructions using the Human CX3CL1/Fractalkine DuoSet ELISA and DuoSet ELISA Ancillary Reagent Kit (R&D Systems, Minneapolis, Minnesota, USA). Supernatants were diluted 1:1 with Reagent Diluent for all measurements and performed in duplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... chemokines and acute phase proteins were simultaneously assessed using the proteome profiler human XL cytokine array kit [ARY005B] from R&D Systems (Abingdon, UK). Cell lysates were obtained from A375 following treatment with 0.1 MOI of RT3D and 0.1 mM talazoparib at 48 hours post-treatment ...
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bioRxiv - Immunology 2023Quote: Supernatants collected from human FM explant experiments were measured for the chemokine IL-8 and cytokine IL-1β by ELISA (R&D Systems, Minneapolis, MN). FM uric acid secretion was measured using a colorimetric assay (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate were cultured in DMEM base medium supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Bioengineering 2024Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
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bioRxiv - Bioengineering 2024Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
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bioRxiv - Bioengineering 2024Quote: Dye-labeled antimicrobial therapeutics (VHHα-(ABD)2-(EEG)6-Sx-POL-Cy7 or VHH16-ABD-(EEG)6-S17-PNT4-sulfo-Cy7) were incubated with recombinant human ADAM10 (R&D Systems, MN, U.S.A.) at 10 μM and 250 nM final concentrations respectively ...
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bioRxiv - Bioengineering 2024Quote: The effective concentration of IL-15 in the samples was assessed using the Human IL-15 Quantikine ELISA Kit (R&D Systems, MN, USA). All subsequent steps after sample collections were carried out following the instructions provided by the kit ...
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bioRxiv - Bioengineering 2024Quote: ... foregut cells in the 24-well plate or on the pillar plate were cultured in advanced DMEM/F12 supplemented with 5 ng/mL recombinant human FGF basic/FGF2/bFGF (R&D systems; 233-FB), 10 ng/mL recombinant human VEGF-165 (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were subsequently washed with PBS without Ca2+ and Mg2+ and then incubated with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2 or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Cell Biology 2020Quote: ... diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat. MAB933, R&D System, Minneapolis, MS, USA) diluted 1:33 in BSA 1% in PBS without Ca2+ and Mg2+for 1h at RT or with negative isotype control mouse IgG2a (cat ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were stained with 1:75 Anti-Hu Osteopontin (SPP1) eFluor 660 (eBioscience; Cat. No. 50-9096-42) or 1:75 Human CCL13 488 (R&D Systems; Cat. No. IC327G) or their isotype controls Mouse IgG1 Control Alexa Fluor 488 conjugated (R&D Systems ...
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bioRxiv - Immunology 2019Quote: ... ELISAs were performed according to the manufacturer’s instructions for IL-10 (Quantikine®HS ELISA Human IL-10 Immunoassay, R&D Systems, Inc., Cat# HS100B), sCD14 (Quantikine® ELISA Human sCD14 Immunoassay ...
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bioRxiv - Genomics 2020Quote: ... the EBs were dissociated with trypsin-EDTA and reaggregated for 40 hr in serum-free differentiation media with the addition of 8ng/ml human activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R & D Systems Cat# 314-BP) ...
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bioRxiv - Cancer Biology 2020Quote: ... and experimental samples were stained with a 30 nM concentration of either commercially available anti-human GPC3 Phycoerythrin (PE)-conjugated mouse antibody (R&D Systems, Inc. Minneapolis, MN) or TAB-H14 antibody conjugated to Alexa Fluor 488 (AF-488 ...
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bioRxiv - Biochemistry 2020Quote: ... transferred membrane was reacted with first antibodies as follows: anti-mouse and human CHL1 antibody (AF2147 and MAB2126; R&D systems, MN; 1 μg/ml), HRP-conjugated anti-mouse and human CHL1 antibody (described above) ...
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bioRxiv - Microbiology 2020Quote: ... Caco2 cells were pre-treated for 30 min at 37°C with goat antibody directed against the human ACE2 or DDP4 ectodomain (R&D Systems, Wiesbaden-Nordenstadt, Germany). Then ...
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bioRxiv - Cancer Biology 2021Quote: ... washed in PBS and resuspended in FACS buffer containing Human Fibroblast Activation Protein alpha PE-conjugated Antibody (FAP-PE) (R&D systems FAB3715P, Clone # 427819) on ice in darkness for 30min ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... plates were incubated for 2 h at RT with 500 ng/mL (25 ng/well) recombinant biotinylated human ACE-2 (R&D Systems, Cat# BT933-020) diluted in PBSTBA ...
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bioRxiv - Microbiology 2020Quote: ... cell-free supernatants were collected and used to evaluate the concentrations of TGF-β1 with human and rat TGF-β1 ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2022Quote: ... freshly dissociated cells were plated at 10,000 cells per well in 100 μL of BTIC medium into a 96-well flat bottom plate in the presence or absence of 10 μg/mL recombinant human biglycan (R&D Systems, 2667-CM-050). We monitored cell growth using a real-time cell imaging system (IncuCyte live-cell ESSEN BioScience Inc) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and incubated with recombinant SARS-CoV-2 Spike protein receptor binding domain (RBD) fused to the human IgG1 Fc domain (R&D Systems 10499-CV-100) or control recombinant human IgG1 Fc protein (R&D Systems 10499-CV-100 ...
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bioRxiv - Physiology 2020Quote: ... The measurement was performed by Enzyme-Linked Immunosorbent Assay (ELISA) using the Human PCSK9 Quantikine ELISA kit (R&D Systems, Catalog no. DPC900, Minneapolis, USA) according to kit instructions ...
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bioRxiv - Bioengineering 2022Quote: ... and Alexa Fluor 647-conjugated Human NCAM-1/CD56 (MC-480) live stain (catalog #FAB24081R-100UG) were purchased from R&D Systems (Minneapolis, MN, USA) as well as Human Three Germ Layer 3-Color Immunocytochemistry Kit (catalog #SC022 ...
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bioRxiv - Cell Biology 2022Quote: The relative levels of angiogenesis-related cytokines in the MSC-CM were analysed using the Proteome Profiler Human Cytokine Array Kit from R&D systems (Abingdon, UK, ARY022B) per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... To assess the cytokine profile of the different conditions plasma and PBMC conditioned media from 3 donors were pooled separately and subjected to the Proteome Profiler Assay Human XL Cytokine Array Kit (R&D Systems, Minneapolis, MN, USA). The assay was performed according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were seeded in 6 well plates and then mock-treated or treated with 5000 U/mL of human recombinant IFNβ (R&D systems, 8499-IF/CF) for 24 hours.
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bioRxiv - Microbiology 2023Quote: ... Media was replaced with DMEM containing gentamicin and ciprofloxacin HCl alone or containing antibiotics and 10% NCS and/or 200 ng/mL Recombinant Human Insulin growth factor 1 (R&D Systems Cat#: 291-G1) for indicated minpost-treatment ...
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bioRxiv - Immunology 2023Quote: Cells were stimulated for 18-24h in complete medium at 37˚C with addition of 50- 100U/ml human IFNγ (R&D Systems #285-IF-100).
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bioRxiv - Cancer Biology 2023Quote: ... spheres incubated under non adherent conditions for 5 days were challenged with the addition of 500 ng/ml human recombinant Netrin-1 (R&D Systems #6419-N1-025) for a period of 20 minutes before collection ...
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bioRxiv - Cell Biology 2022Quote: ... CXCL1 levels were measured after 3 h and 6 h using the Human CXCL1/GROα DuoSet® ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... IMR90 fibroblasts at 70-80 % confluent were serum starved overnight and treated with 50ng/ml of recombinant human PDGF-DD (R&D system, 1159-SB-025) for 24h.
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bioRxiv - Microbiology 2023Quote: ... The concentration of IL-8 released in basolateral culture media were assayed by ELISA using the human ELISA development kits (DuoSet DY208, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... according to the manufacturer’s protocol and reaggregated for 40h in serum-free differentiation media with the addition of 8 ng/ml human Activin A (R&D Systems Cat#. 338-AC), 0.5 ng/ml human BMP4 (R&D Systems Cat# 314-BP) ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
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bioRxiv - Immunology 2024Quote: ... Total transferrin was kept constant at 1.2 mg/mL by adjusting human apotransferrin (unbound transferrin) concentrations (R&D systems, 3188-AT-001G/Sigma Aldrich, T1147) accordingly ...
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bioRxiv - Microbiology 2023Quote: ... The levels of CD83 (pg/ml) secreted in the culture medium were determined with the Human CD83 DuoSet ELISA kit (R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... minus 10 nM prostaglandin E2 and 2.5 μM Thiazovinin and with added human recombinant Wnt3A 50 ng/mL (R&D Systems, #5036-WN-010/CF), R-Spondin-1 250 ng/mL (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... lysates of specific conditions were collected and the relative phosphorylation status of 37 kinase phosphorylation sites was evaluated by using a Human Phospho-kinase Array kit (ARY003C, R&D Systems, Minneapolis, MN, USA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and differentiated into M1 or M2 macrophages by incubation for 7 days in the presence of 100 ng/mL recombinant human granulocyte-macrophage colony-stimulating factor (GM-CSF) or macrophage-colony stimulating factor (M-CSF) (R&D Systems, Minneapolis, MN, USA), respectively.