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• Gadolinium-based nanoparticles AGuIX and their combination with ionizing radiation trigger AMPK-dependent proinflammatory reprogramming of tumor-associated macrophages

  Zeinaf Muradova, et al., bioRxiv - Immunology 2024

  Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
• Targeting PD-L1 in solid cancer with myeloid cells expressing a CAR-like immune receptor

  Kayla Myers Chen, et al., bioRxiv - Immunology 2024

  Quote: ... PE human EGFR from R&D Systems; APC mouse PD-L1 from Tonbo Biosciences ...
• Functional impairment of “helpless” CD8⁺ memory T cells is transient and driven by prolonged but finite cognate antigen presentation

  Verena van der Heide, et al., bioRxiv - Immunology 2024

  Quote: ... samples were pre-treated for 1h at 37°C with 0.1 mM TAPI-2104 (R&D Systems) prior to subsequent incubation with gp33 peptide as detailed above in the continuous presence of TAPI-2 ...
• First-in-class Transactivator-Free, Doxycycline-inducible IL-18-engineered CAR-T cells for relapsed/refractory B-cell lymphomas

  Pedro Justicia-Lirio, et al., bioRxiv - Immunology 2024

  Quote: ... Anti-hIL-18 Propeptide-PE (74801, 1:20, R&D systems), pCD3ζ-PE (Tyr142 ...
• Single-cell RNA sequencing reveals recruitment of the M2-like CCL8^high macrophages in Lewis lung carcinoma-bearing mice following hypofractionated radiotherapy

  Haonan Yang, et al., bioRxiv - Immunology 2024

  Quote: ... Cytokines were quantified according to the manufacturer’s protocol (Luminex Mouse Discovery Assay, R&D Systems). CSF1 ...
• Chitinase 3-like-1 (CHI3L1) Inhibits Innate Anti-Tumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα and CD24-Siglec10-Mediated Phagocytosis

  Bing Ma, et al., bioRxiv - Immunology 2024

  Quote: ... They were then incubated with recombinant M-CSF (50ng/ml) and rCHI3L1 (R&D Systems, 250ng/ml) alone or together ...
• Chitinase 3-like-1 (CHI3L1) Inhibits Innate Anti-Tumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα and CD24-Siglec10-Mediated Phagocytosis

  Bing Ma, et al., bioRxiv - Immunology 2024

  Quote: ... They were then incubated with recombinant (r) CHI3L1 (500ng/ml, R&D Systems, #2599-CH) in the presence of anti-CHI3L1 antibody (FRG ...
• Body-wide genetic deficiency of poly(ADP-ribose) polymerase 14 sensitizes mice to colitis

  Madhukar Vedantham, et al., bioRxiv - Immunology 2024

  Quote: ... or 10 ng/mL of TNFα (210-TA, R&D systems). Wells with fresh media without any inflammatory stimuli served as control ...
• CD271 restrains the B1b cell antibody response in a T cell dependent manner

  Marie-Ève Lebel, et al., bioRxiv - Immunology 2024

  Quote: ... with or without 10 µg/ml Fc proteins (R&D Systems Inc.). Mouse T cells were enriched with EasySep™ Mouse T Cell Enrichment Kit (Miltenyi Biotec ...
• Immunothrombolytic monocyte-neutrophil axes dominate the single-cell landscape of human thrombosis

  Kami Pekayvaz, et al., bioRxiv - Immunology 2024

  Quote: ... anti-MPO (R&D systems, Cat. # AF3667), and anti-Urokinase (Abcam ...
• Protecting Allogeneic Pancreatic Islet Grafts by Engineered Veto

  Yan Qi, et al., bioRxiv - Immunology 2024

  Quote: Supernatants harvested from mixed lymphocyte cultures established from three mice of each group were tested using commercial assay kits (e.Bioscience, R&D Systems, Bender MD Systems) for the presence of interferon-γ ...
• Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection

  Mack B. Reynolds, et al., bioRxiv - Immunology 2024

  Quote: ... IL-6 (DY 406, R&D Systems), IL-β (DY 401 ...
• Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection

  Mack B. Reynolds, et al., bioRxiv - Immunology 2024

  Quote: ... TNF-α (DY 410, R&D Systems), IL-6 (DY 406 ...
• Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection

  Mack B. Reynolds, et al., bioRxiv - Immunology 2024

  Quote: ... IL-β (DY 401, R&D Systems), and IFN-β (DY 8234-05 ...
• Type I interferon governs immunometabolic checkpoints that coordinate inflammation during Staphylococcal infection

  Mack B. Reynolds, et al., bioRxiv - Immunology 2024

  Quote: ... and IFN-β (DY 8234-05, R&D Systems) were measured by enzyme-linked immunosorbent assay (ELISA ...
• Recapitulating memory B cell responses in a Lymphoid Organ-Chip to evaluate mRNA vaccine boosting strategies

  Raphaël Jeger-Madiot, et al., bioRxiv - Immunology 2024

  Quote: The chemokine CXCL13 was measured in LO chip effluent using a human CXCL13 ELISA assay (Quantikine, R&D Systems #DY801), following the manufacturer’s instructions.
• Chitinase 3-like-1 (CHI3L1) Inhibits Innate Anti-Tumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα and CD24-Siglec10-Mediated Phagocytosis

  Bing Ma, et al., bioRxiv - Immunology 2024

  Quote: ... and Siglec10 (R&D Systems, MAB7775) overnight at 4°C ...
• Chitinase 3-like-1 (CHI3L1) Inhibits Innate Anti-Tumor and Tissue Remodeling Immune Responses by Regulating CD47-SIRPα and CD24-Siglec10-Mediated Phagocytosis

  Bing Ma, et al., bioRxiv - Immunology 2024

  Quote: ... Siglec10 (R&D Systems, MAB7775), HMGB1 (Abcam ...
• Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics

  Youxi Liu, et al., bioRxiv - Immunology 2024

  Quote: ... primary dFBs were treated with IFNγ (5 ng/ml, R&D Systems) or PBS control for 2 hours ...
• Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics

  Youxi Liu, et al., bioRxiv - Immunology 2024

  Quote: ... dFBs were treated with either recombinant mouse IFNγ (5 ng/ml, R&D Systems, Minneapolis, MN, USA) for 8 or 24 hours before being subjected to qRT-PCR or ELISA analysis.
• Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics

  Youxi Liu, et al., bioRxiv - Immunology 2024

  Quote: ... Protein lysates were subjected to enzyme-linked immunosorbent assay (ELISA) using mouse DuoSet® ELISA commercial kits (R&D Systems, Minneapolis, MN, USA), following manufacturer instructions.
• Influenza A Virus Disruption of Dendritic Cell-Natural Killer Cell Crosstalk Impacts Activation of Helper and Cytotoxic T cell Subsets

  Connor A. Morson, et al., bioRxiv - Immunology 2024

  Quote: ... suspended and cryopreserved at -80°C in 90% (v/v) fetal bovine serum (FBS; R&D Systems, Minneapolis, MN) and 10% (v/v ...
• Aspergillus-mediated allergic airway inflammation is triggered by dendritic cell recognition of a defined spore morphotype, a process that can be targeted via antifungal therapeutics

  EL Houlder, et al., bioRxiv - Immunology 2024

  Quote: ... or DuoSets following manufacturer’s instructions (BioLegend, eBio-science, BD, R&D Systems, and PeproTech).
• Differentially polarized macrophages show diverse proangiogenic characteristics under normo- and hyperglycemic conditions

  Mahnaz Shariatzadeh, et al., bioRxiv - Immunology 2024

  Quote: ... M-CSF + IL-6 (50 ng/ml, # 206-IL-050/CF, R&D systems), or M-CSF + IFN-γ (40 ng/ml ...
• Identification of a distinct cluster of GDF15^high macrophages exhibiting anti-inflammatory activities

  Chaochao Dai, et al., bioRxiv - Immunology 2024

  Quote: Recombinant GDF15 (#10596-GD) and recombinant interferon (IFN)-γ (#585-IF) were purchased from R&D Systems (Minneapolis, MN, USA). Recombinant human granulocyte-macrophage colony stimulating factor (GM-CSF ...
• Inheritance of old mitochondria controls early CD8⁺ T cell fate commitment and is regulated by autophagy

  Mariana Borsa, et al., bioRxiv - Cell Biology 2024

  Quote: ... and recombinant human or murine Fc-ICAM-1 (10 μg/ml) (R&D Systems) coated plates ...
• Lysosomal degradation of ACVR1-Activin complexes negatively regulates signaling of Activins and Bone Morphogenetic Proteins

  Warren W. Hom, et al., bioRxiv - Cell Biology 2024

  Quote: ... and anti-ALK4 antibody (MAB222) were purchased from R&D Systems. Activin E was custom produced ...
• Reduction of chemokine CXCL9 expression by omega-3 fatty acids via ADP-ribosylhydrolase ARH3 in MIN6 insulin-producing cells

  Youngki You, et al., bioRxiv - Cell Biology 2024

  Quote: The levels of CXCL9 secreted to the media by MIN6 cells were quantified by Mouse DuoSet ELISA kit (R&D Systems). Polystyrene 96-well plates were coated with 100 μL of 200 ng/mL unconjugated goat anti-mouse CXCL9 antibody in PBS at room temperature overnight ...
• A genome-wide screen links peroxisome regulation with Wnt signaling through RNF146 and tankyrase

  Jonathan T. Vu, et al., bioRxiv - Cell Biology 2024

  Quote: ... Cells were treated with either BSA or human recombinant WNT3a (5036-WN-010, R&D Systems) 24 hours later ...
• Evaluation of Autophagy in conjunctival fibroblasts

  Parvaneh Mehrbod, et al., bioRxiv - Cell Biology 2024

  Quote: Horseradish Peroxidase (HRP)-conjugated anti-rabbit IgG and anti-goat IgG (R&D Systems, Minneapolis, MN, USA)
• Med12 cooperates with multiple differentiation signals to enhance embryonic stem cell plasticity

  Max Fernkorn, Christian Schröter, bioRxiv - Cell Biology 2024

  Quote: ... Anti-SOX17 (R&D systems, Cat.: AF1924)) were diluted 1:200 in PBT-BSA and incubated with the cells overnight at 4 °C ...
• Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

  Emily M. Carvalho, et al., bioRxiv - Bioengineering 2024

  Quote: ... 20 ng/mL EGF (R&D Systems), and 20 ng/mL FGF (R&D Systems) ...
• Viscoelastic high-molecular-weight hyaluronic acid hydrogels support rapid glioblastoma cell invasion with leader-follower dynamics

  Emily M. Carvalho, et al., bioRxiv - Bioengineering 2024

  Quote: ... and 20 ng/mL FGF (R&D Systems). For experiments on HA gels ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... Cyr61 concentration was determined using a human Cyr61 Quantikine ELISA (R&D Systems, Minneapolis, MN) following the manufacturer’s instructions.
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... 0.1% v/v FBS with or without 100 ng/ml Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN). Medium change was performed at day 3 and images were taken at 3 and 5 days using a Keyence BZ-X800 Fluorescence Microscope (Keyence ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... The following primary antibodies and secondary antibodies were used (staining durations provided): CYR61 (R&D Systems, Minneapolis ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: A total of 1 μg Cyr61 (Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) was dissolved in GelMA/fibrin hydrogel containing thrombin and transferred into a cylinder-shaped mold with a liquid capacity of 8.6 μl and 1 mm diameter with 1 mm thickness ...
• 3D-printed, biomimetic, conductive MXene-microfiber composite scaffolds enhance the axonal growth-promoting characteristics of electrical stimulation

  I. Woods, et al., bioRxiv - Bioengineering 2024

  Quote: ... N21 max (R&D Systems, UK), and 0.2% B27 supplement ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... Different concentrations of endogenous Cyr61 (0-200 ng/ml; Recombinant Human Cyr61/CCN1 Fc Chimera Protein, carrier-free; catalog no.: 4055-CR; R&D Systems, Minneapolis, MN) and TGFβ (0 or 10 ng/ml ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... and 10 ng/ml fibroblast growth factor-2 (R&D Systems, Minneapolis, MN). For pellet cultures ...
• Cyr61 delivery promotes angiogenesis during bone fracture repair

  Annemarie Lang, et al., bioRxiv - Bioengineering 2024

  Quote: ... and TGFβ (0 or 10 ng/ml; rhTGF-beta1; R&D Systems, Minneapolis, MN) were added to the medium ...
• Intermittent Cyclic Stretch of Engineered Ligaments Drives Hierarchical Collagen Fiber Maturation in a Dose- and Organizational-Dependent Manner

  Leia D. Troop, Jennifer L. Puetzer, bioRxiv - Bioengineering 2024

  Quote: ... The assay was run according to manufactures protocol with recombinant LOXL2 (R&D Systems) used as a positive control and results normalized to sample DNA determined via the Quant-iT Picogreen dsDNA assay.
• A PTER-dependent pathway of taurine metabolism linked to energy balance

  Wei Wei, et al., bioRxiv - Biochemistry 2024

  Quote: ... Recombinant GDF15 (957-GD) was purchased from R&D systems. Anti-GFRAL neutralizing antibody and control IgG antibody36 were obtained from Eli Lilly ...
• Chronic IL-1-induced DNA double-strand break response in hippocampal neurons drives cognitive deficits

  Belloy Marcy, et al., bioRxiv - Animal Behavior and Cognition 2024

  Quote: Serum IL-1β levels were assessed by ELISA according to the manufacturer’s instructions (Quantikine ELISA kit, R&D Systems). Microdissected hippocampi were homogenized with the FastPrep-24™ Classic Instrument in Lysing MatrixD tubes (MP Biomedicals ...
• A PTER-dependent pathway of taurine metabolism linked to energy balance

  Wei Wei, et al., bioRxiv - Biochemistry 2024

  Quote: ... blood plasma was collected at 9 am and ELISA kits were used following manufacturer’s instructions (Leptin: Crystal Chem, 90030; GLP-1: Sigma, EZGLP1T-36K; GDF-15: R&D Systems, MGD150 ...
• UDP-6-glucose dehydrogenase in hormonally responsive breast cancers

  Meghan J. Price, et al., bioRxiv - Cancer Biology 2024

  Quote: ... and anti-GPER/GPR30 (AF5534, R&D Systems, Minneapolis, MN), and anti- β-actin (8H10D10 ...
• Circadian rhythm disruption alters mammary gland morphology and accelerates “cold” aggressive tumorigenesis through a LILRB4-dependent pathway

  Olajumoke Ogunlusi, et al., bioRxiv - Cancer Biology 2024

  Quote: ... The cytokine array was performed using the Proteome Profiler Mouse XL Cytokine Array kit (R&D Systems, ARY028). The blood samples were kept undisturbed for 30 minutes at room temperature before being spun at 2000 x g for 10 minutes at 4°C ...
• Structural insights into CXCR4 modulation and oligomerization

  Kei Saotome, et al., bioRxiv - Biochemistry 2024

  Quote: ... Gi-bound complex sample) or mixed with 0.5 mg/mL CXCL12 (Recombinant Human/Rhesus Macaque/Feline CXCL12/SDF-1 alpha, R&D Systems Catalog #: 350-NS-050/CF), or 1 mM AMD3100 (AMD 3100 octahydrochloride ...
• Structural insights into CXCR4 modulation and oligomerization

  Kei Saotome, et al., bioRxiv - Biochemistry 2024

  Quote: ... or 1 mM AMD3100 (AMD 3100 octahydrochloride, R&D Systems Catalog #: 3299), or ∼1 to 1.5 mg/mL REGN7663 Fab and incubated on ice for ∼1 hour ...
• The activation chain of the broad-spectrum antiviral bemnifosbuvir at atomic resolution

  Aurélie Chazot, et al., bioRxiv - Biochemistry 2024

  Quote: ... carboxylesterase 1 (CES1) and trans-epoxy-succinyl-L-leucylamino(4-guanidino)butane (E-64) were purchased from R&D Systems. The respective genes coding for the human recombinant enzymes Histidine triad nucleotide binding protein 1 (HINT1) ...