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Citations for R&D Systems :
601 - 650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... CD3+ T cells were isolated from the spleens of female OT-I (CD45.1+/CD45.2+) mice using CD3+ T cell enrichment columns (R&D Systems, MTCC25) and co-cultured with the peptide-pulsed splenocytes for 3 days ...
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bioRxiv - Immunology 2024Quote: ... treatment wells received 10 ng/ml (for migration assays, co-culture assays proteomic screening) and or 1 ng/ml (for RNA-seq) of rhIFNγ (R&D systems).
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bioRxiv - Immunology 2024Quote: The spinal cord sections were retrieved by Universal Antigen Retrieval Kit (R&D Systems) in a steamer for 15 min at 100°C ...
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bioRxiv - Immunology 2024Quote: ... Slides of aortic cross-sections were prepared and stained for elastin (Van Gieson’s Solution, catalog no. s289; Poly Scientific R&D Systems, Bay Shore, NY) smooth muscle actin (monoclonal anti-actin α-smooth muscle ...
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bioRxiv - Immunology 2024Quote: ... were grown in DMEM supplemented with 10% FBS and 100 U/ml penicillin–streptomycin in the presence of macrophage colony-stimulating factor (M-CSF, 10 ng/ml, R&D Systems 416-ML-050), as described earlier in our studies ...
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bioRxiv - Immunology 2024Quote: ... the cells were incubated with IFNγ (100 ng/ml, R&D Systems 485-MI-100) and LPS (100 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... on a MAGPIX Instrument (R&D Systems) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Lymphocytes from SP and draining LNs were cultured in the presence of IRBP-p (10 μg/ml) and IL-23 (R&D systems, Minneapolis, MN) (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Cells were treated with fresh A549 media with or without human IFN-β recombinant protein (R&D Systems™ 8499IF010) at the indicated concentrations ...
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bioRxiv - Immunology 2024Quote: ... and TNFα (1ng/ml, R&D Systems) for 48 hrs ...
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bioRxiv - Immunology 2024Quote: ... and anti-B220 (Rat anti-mouse; monoclonal: RA3-6B2 clone; dilution 1:100 in blocking solution; R&D systems). Secondary antibody staining was performed for 50min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were filtered through a screen and washed with 5 mM EDTA in EHAA + 2.5% FBS (R&D Systems, Cat. No.: S12450). The 8 popliteal LNs digested for dendritic cells isolation were then stained with CD11c (N418) ...
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bioRxiv - Genomics 2024Quote: ... cells were also treated with or without TNFα (R&D systems, 100 ng/ml) at 48 h after transfection and harvested at 72 h after transfection (24 h after TNFα treatment).
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bioRxiv - Genomics 2024Quote: ... The human RPTEC with early passages were seeded at 8 × 105 cells on 10 cm culture dish at the day before the assay and treated with or without TNFα (R&D systems, 100 ng/ml) at 3 h before the fixation ...
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bioRxiv - Immunology 2024Quote: ... Each human FcγR (R&D Systems) was captured on immobilized anti-His antibodies at 100-200 RU level ...
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bioRxiv - Immunology 2024Quote: ... Anti-histidine antibody (R&D Systems) was covalently immobilized on a CM5-S sensor chip flowcell (Fc2 ...
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bioRxiv - Immunology 2024Quote: ... human IL-15 (247-IL/CF; R&D Systems), human TGFβ1 (580706 ...
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bioRxiv - Immunology 2024Quote: ... Adaptive NK cell-positive donors were identified by anal-ysis of NKG2C (FAB138P or FAB138A antibodies, R&D Systems) and FcεRIγ (FCABS400F antibody ...
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bioRxiv - Immunology 2024Quote: ... Mouse and human ACE2 protein levels were quantified from lung homogenates by ELISA (R&D Systems #DY3437, #DY933).
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bioRxiv - Immunology 2024Quote: ... 3 ng/mL GMCSF (R&D systems) and 200 ng/mL Flt3-L (R&D systems ...
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bioRxiv - Immunology 2024Quote: ... recombinant human IL-2 (R&D systems, cat # 202-IL-010/CF), and SIINFEKL peptide (0.5ug ...
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bioRxiv - Immunology 2024Quote: Primary antibodies used: CD64 (R&D Systems, AF2074) 1:200 dilution ...
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bioRxiv - Immunology 2024Quote: ... and creatinine (R&D Systems, Minneapolis, USA) were measured by following the kit instructions ...
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bioRxiv - Immunology 2024Quote: ... Cytokine protein levels were measured by ELISA (IL-17E, IL-5, and IL-13 Duo-Set, R&D Systems). Cytokine levels were normalized to total protein concentration in the tissue lysates using the DC Protein Assay (BioRad) ...
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bioRxiv - Immunology 2024Quote: ... anti-AMPKα2 (#AF2850, diluted at 1:1000) and anti-IL6 (#AB-206-NA, diluted at 1:500) antibodies from R&D Systems (Minneapolis, MN, USA). Secondary antibodies used for immunofluorescence labelling of fixed cells were goat anti-rabbit antibodies conjugated to Alexa Fluor 488 (#11034 ...
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bioRxiv - Immunology 2024Quote: ... PE human EGFR from R&D Systems; APC mouse PD-L1 from Tonbo Biosciences ...
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bioRxiv - Immunology 2024Quote: ... samples were pre-treated for 1h at 37°C with 0.1 mM TAPI-2104 (R&D Systems) prior to subsequent incubation with gp33 peptide as detailed above in the continuous presence of TAPI-2 ...
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bioRxiv - Immunology 2024Quote: ... Anti-hIL-18 Propeptide-PE (74801, 1:20, R&D systems), pCD3ζ-PE (Tyr142 ...
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bioRxiv - Immunology 2024Quote: ... Cytokines were quantified according to the manufacturer’s protocol (Luminex Mouse Discovery Assay, R&D Systems). CSF1 ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated with recombinant M-CSF (50ng/ml) and rCHI3L1 (R&D Systems, 250ng/ml) alone or together ...
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bioRxiv - Immunology 2024Quote: ... They were then incubated with recombinant (r) CHI3L1 (500ng/ml, R&D Systems, #2599-CH) in the presence of anti-CHI3L1 antibody (FRG ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed with FACS buffer (PBS + 1% bovine serum albumin (BSA)) and 1 mL aliquots of cells were incubated with 1 µg of sheep anti-human dystroglycan antibody (R&D Systems, Cat. No. AF6868) for 30 minutes on ice ...
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bioRxiv - Microbiology 2024Quote: ... permeabilised in 0.1% triton X-100 (FC005, R&D systems), washed again and labelled with DAPI (1 μg/ml) ...
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bioRxiv - Microbiology 2024Quote: Quantification of cytokines present in cell culture supernatants was performed by ELISA using the DuoSet ELISA kit (R&D Systems) for TNF-α ...
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bioRxiv - Immunology 2024Quote: ... goat anti-S100A8 (R&D Systems AF3059, 1:200), chicken anti-NeuN (Millipore Sigma ABN91 ...
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bioRxiv - Immunology 2024Quote: ... 1% P/S containing CCL17 (800ng/ml, R&D Systems, #529-TR), when indicated ...
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bioRxiv - Immunology 2024Quote: ... IL-12 and recombinant human TGF-β1 were purchased from R&D Systems (Abingdon, UK).
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bioRxiv - Immunology 2024Quote: ... IL-25 concentration was determined by ELISA (R&D Systems) and/or Luminex (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... CCL17 (800ng/ml, R&D Systems, #529-TR) was added during the differentiation process ...
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bioRxiv - Immunology 2024Quote: ... 2mM L-Glutamine and 20ng/mL of recombinant human IL-3 (R&D systems). Cas9 and gRNA components were assembled into RNP per the manufacturer’s instructions and transfected into pDC 16 hrs p.c ...
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bioRxiv - Immunology 2024Quote: ... Murine splenic CD3+ T cells were isolated using CD3+ T cell enrichment columns (R&D Systems, MTCC25). T cells were cultured at 37C in RPMI-1640 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... and Siglec10 (R&D Systems, MAB7775) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of pre-cleared lysates was incubated with 50 µL Dynabeads Protein G (30 mg/ml) and 5 µg Regnase-1 (R&D systems) or Regnase-3 (Helmholtz Munich Monoclonal Antibody Core Facility ...
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bioRxiv - Immunology 2024Quote: ... plus 10% FBS (R&D Systems), 1X GlutaMAX (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... 1x106 cells then stained in 200 µL on ice for 30 minutes with: Alexa Fluor® 405 anti-IFNα/β R1 (1µg, FAB245V, R&D Systems), PE anti-IFNα R2 (1µg ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 488 anti-IFNγ R1/CD119 (1µg, FAB6731G, R&D Systems) and APC anti-IFNγ R2 (0.5µg ...
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bioRxiv - Immunology 2024Quote: ... 300 μL of each 1:10 dilution mix (containing cells and ADC) was added to 3mL aliquots of complete mouse or human methylcellulose media (R&D Systems; HSC007 and HSC005, respectively) and vortexed thoroughly ...
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bioRxiv - Immunology 2024Quote: ... anti-CXCL10 or anti-CXCL11 mAb (R&D systems) were added to the lower compartment prior to start of the assay.
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bioRxiv - Immunology 2024Quote: ... Cryosections were stained with a monoclonal rat IgG2a anti-mouse NKp46/NCR1 (R&D Systems, clone # 29A1.4) and revealed using a donkey AF568-conjugated anti-rat pAb (Abcam) ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ in the culture supernatant was measured by ELISA (DuoSet, R&D Systems).