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• Semaglutide interferes with postnatal growth in a diet-dependent manner

  Amélie Joly, et al., bioRxiv - Physiology 2024

  Quote: ... supernatants were collected and used to measure IGF-1 levels by Mouse/Rat IGF-1 Quantikine ELISA (R&D systems, MG100). BCA assay was performed to normalize IGF-1 concentration to protein concentration in the liver ...
• ACKR3 agonism induces heterodimerization with chemokine receptor CXCR4 and attenuates platelet function

  Valerie Dicenta-Baunach, et al., bioRxiv - Physiology 2024

  Quote: ... the platelets were incubated at 4°C over night with antibodies against CXCR4 (MAB172, Clone # 44716, R&D Systems, Minneapolis, Minnesota, USA), ACKR3 (ab72100 ...
• Hepatic Nrf1 (Nfe2l1) promotes VLDL dependent liver defense against sepsis

  Michael J. Trites, et al., bioRxiv - Physiology 2024

  Quote: ... Plasma IGF1 levels were determined by ELISA according to manufacturer’s instructions (R&D Systems, catalog #DY791).
• The metabolic benefits of thermogenic stimulation are preserved in aging

  Duraipandy Natarajan, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... anti-UCP1 (1:1000, R&D Systems #MAB6158), anti-glycerol kinase (GYK ...
• ACKR3 agonism induces heterodimerization with chemokine receptor CXCR4 and attenuates platelet function

  Valerie Dicenta-Baunach, et al., bioRxiv - Physiology 2024

  Quote: ... or corresponding IgG controls (Mouse IgG2B Isotype Control MAB004 R&D Systems, Minneapolis ...
• Dynamic Single Cell Transcriptomics Defines Kidney FGF23/KL Bioactivity and Novel Segment-Specific Inflammatory Targets

  Rafiou Agoro, et al., bioRxiv - Physiology 2024

  Quote: Wild type C57BL/6 10-week-old female mice were treated daily with recombinant tumor necrosis factor-like weak inducer of apoptosis (TWEAK 1237-TW; R&D Systems; 2 ug per mouse) for 4 days followed by a single i.p ...
• Complementing Muscle Regeneration: Fibro-Adipogenic Progenitor and Macrophage-Mediated Repair of Elderly Human Skeletal Muscle

  Jonas Brorson, et al., bioRxiv - Physiology 2024

  Quote: ... PDGFR-α (goat anti human, 1:300, cat. no.: AF-307-NA; RRID: AB_354459, R&D Systems, Bio-Techne), and C3 (rabbit anti human ...
• Model-guided design of microRNA-based gene circuits supports precise dosage of transgenic cargoes into diverse primary cells

  Kasey S. Love, et al., bioRxiv - Synthetic Biology 2024

  Quote: ... and 30 IU/mL recombinant human IL-2 (R&D Systems). Following isolation ...
• A Novel Role of Hyaluronan and its Membrane Receptors, CD44 and RHAMM in Obesity-Related Glomerulopathy

  Bingxue Qi, et al., bioRxiv - Physiology 2024

  Quote: ... 1ng/ml TNF-α and 1ng/ml IL-6 (R&D systems, Abingdon, UK). D-Mannitol (Sigma ...
• Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

  Matteo Napoli, et al., bioRxiv - Physiology 2024

  Quote: ... intrascrotal (i.s.) injection of rmTNF-α (500ng, R&D Systems) was applied to WT and Mrp14-/- mice in order to induce an acute inflammation in the cremaster muscle ...
• Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

  Matteo Napoli, et al., bioRxiv - Physiology 2024

  Quote: ... VitroCom) were coated with a combination of rmE-selectin (CD62E Fc chimera; 20µg mL−1; R&D Systems), rmICAM-1 (ICAM-1 Fc chimera ...
• Cytosolic S100A8/A9 promotes Ca²⁺ supply at LFA-1 adhesion clusters during neutrophil recruitment

  Matteo Napoli, et al., bioRxiv - Physiology 2024

  Quote: ... rmICAM-1 (ICAM-1 Fc chimera; 15µg mL−1; R&D Systems), and rmCXCL1 (15µg mL−1 ...
• Asparagine couples group A Streptococcal metabolism to virulence

  Abhinay Sharma, et al., bioRxiv - Microbiology 2024

  Quote: ... The cell-free supernatants were incubated with 1 ng/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2 h ...
• Asparagine couples group A Streptococcal metabolism to virulence

  Abhinay Sharma, et al., bioRxiv - Microbiology 2024

  Quote: ... The cell-free supernatants were incubated with an equal volume of 1 mg/ml of recombinant human IL-8 (R&D Systems, USA) at 37°C for 2h ...
• Asparagine couples group A Streptococcal metabolism to virulence

  Abhinay Sharma, et al., bioRxiv - Microbiology 2024

  Quote: ... estimated using the Human IL-8 Quantikine ELISA kit (R&D Systems, USA).
• STAT5B SH2 variants disrupt mammary enhancers and the stability of genetic programs during pregnancy

  Hye Kyung Lee, Chengyu Liu, Lothar Hennighausen, bioRxiv - Genetics 2024

  Quote: ... STAT5B (R&D systems, AF1584 ...
• Loss of SPNS1, a lysosomal transporter, in the nervous system causes dysmyelination and white matter dysplasia

  Yoshinobu Ichimura, et al., bioRxiv - Cell Biology 2024

  Quote: ... LAMP-1 (AF4800, R&D Systems, Minneapolis, MN, USA; 1:200), and GALECTIN-3 (sc-23938 ...
• JUNB O-GlcNAcylation-mediated promoter accessibility of metabolic genes modulates distinct epithelial lineage in pulmonary fibrosis

  Marie-Therese Bammert, et al., bioRxiv - Cell Biology 2024

  Quote: ... AOs were dissolved away from Cultrex® RGM Type 2 (R&D systems, #3533-010-02) and fixed in 4 % paraformaldehyde (PFA ...
• Leukemia inhibitory factor (LIF) receptor amplifies pathogenic activation of fibroblasts in lung fibrosis

  Hung N. Nguyen, et al., bioRxiv - Cell Biology 2024

  Quote: ... POSTN (Periostin) ELISA kits (R&D Systems); ACTA2 (aSMA ...
• APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

  Sarah E.V. Richards, et al., bioRxiv - Cell Biology 2024

  Quote: ... Primary antibodies for APOE (1:500; R&D Systems, MAB41441), ZO-1 (1:200 ...
• APOE Impacts Lipid Trafficking in Retinal Pigment Epithelium Cells

  Sarah E.V. Richards, et al., bioRxiv - Cell Biology 2024

  Quote: ... Detection antibody (R&D Systems, MAB41441) diluted 1:1000 in blocking buffer (VWR ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... 3 U/mL erythropoietin (R&D Systems USA), 10 μg/mL insulin (StemCell Technologies) ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... 3 IU/ml erythropoietin (R&D Systems) and 1% penicillin/streptomycin ...
• Healthy human induced pluripotent stem cell-derived cardiomyocytes exhibit sex dimorphism even without the addition of hormones

  Sophie E. Givens, et al., bioRxiv - Cell Biology 2024

  Quote: The hiPSCs were cultured in mTesRTM1 (STEMCELL 85850) on 8.68 ug/cm2 (or 0.5 mg per 6 well-plate) Cultrex reduced growth factor (RGF) (R&D Systems 3433-005-01) coated 6-well plates ...
• Primary Human Neutrophils and Monocytes/Macrophages Migrate along Endothelial Cell Boundaries to Optimize Search Efficiency

  Nele Honig, et al., bioRxiv - Cell Biology 2024

  Quote: ... VE-Cadherin Monoclonal Antibody (R&D Systems, MAB9381), α-Catenin (Atlas Antibodies ...
• Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during cell growth arrest

  Francesco Neri, et al., bioRxiv - Cell Biology 2024

  Quote: ... and 100 µg/mL streptomycin (R&D Systems, B21210) at 37°C ...
• Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during cell growth arrest

  Francesco Neri, et al., bioRxiv - Cell Biology 2024

  Quote: ... supplemented with 10% FBS (R&D Systems, S11550H), 100 units/mL penicillin ...
• Identification of small molecule agonists of fetal hemoglobin expression for the treatment of sickle cell disease

  Jian-Ping Yang, et al., bioRxiv - Cell Biology 2024

  Quote: ... The Phase I expansion media contains 50 ng/mL human stem cell factor (R&D Systems), 10 μM dexamethasone (Sigma-Aldrich) ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... Wnt3a was supplemented only on day 1 (25 ng/ml, R&D Systems, #5036-WN/CF). Thereafter ...
• Constant surface area-to-volume ratio during cell growth as a design principle in mammalian cells

  Weida Wu, et al., bioRxiv - Cell Biology 2024

  Quote: ... FL5.12 cells were cultured in the presence of 10 ng/ml IL-3 (R&D Systems). Cell cycle exit in FL5.12 was achieved by washing the cells three times with PBS to remove IL-3 from the cells and by placing the cells in IL-3 free culture media for 48 h ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... FGF4 (500 ng/ml, R&D Systems, #235-F4/CF), CHIR99021 (3 μM ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... Activin A (100 ng/ml, R&D Systems, #338-AC) and an increasing concentration of defined FBS (day 1 ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... Noggin (100 ng/ml, R&D Systems, #6057-NG/CF), EGF (100 ng/ml ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... EGF (100 ng/ml, R&D Systems, #236-EG), CHIR99021 (2 μM ...
• Intestine-on-chip enhances nutrient and drug metabolism and maturation of iPSC-derived intestinal epithelial cells relative to organoids and Transwells

  Renée Moerkens, et al., bioRxiv - Cell Biology 2024

  Quote: ... EGF (100 ng/ml, R&D Systems, #236-EG), B27 (1x ...
• Dynamic and decay kinetics of H3 variants in live cells reveal the pivotal role of HIRA/NSD2 in maintaining the distinct H3.3 specific chromatin landscape

  Vishal Nehru, et al., bioRxiv - Cell Biology 2024

  Quote: ... and HIRAfl/fl Cre-ERT MEF cells were prepared from E12.5 embryos and maintained in Dulbecco’s minimal essential medium (DMEM) supplemented with 10% fetal bovine serum (R&D systems) and 1% antibiotics (Gibco) ...
• Proinflammatory cytokine-induced alpha-cell impairment in human islet microtissues is partially restored by dual incretin receptor agonism

  Kristine Henriksen, et al., bioRxiv - Cell Biology 2024

  Quote: Islet MTs were cultured for 1 day (short-term) or 6 days (long-term) with or without a cytokine mix of recombinant human IL-1β (R&D Systems, USA or Sigma-Alrich, USA), recombinant human IFN-γ (PeproTech ...
• Proinflammatory cytokine-induced alpha-cell impairment in human islet microtissues is partially restored by dual incretin receptor agonism

  Kristine Henriksen, et al., bioRxiv - Cell Biology 2024

  Quote: ... and recombinant human TNF-α (R&D Systems, USA) in a 1:5:5 ratio ...
• Adipogenic differentiation and inflammatory response is orchestrated by regulating euchromatic histone methyltransferases

  Mahua Chakraborty, et al., bioRxiv - Cell Biology 2024

  Quote: Extracellular conditioned media from differentiated 3T3-L1 cells pretreated with or without palmitate and UNC0642 were quantified using mouse IL-6 Quantikine ELISA kit M6000B from R&D Systems as per the manufacturer’s protocol ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... IL-1β (R&D Systems; #AF401-NA); ETS1 (Cell Signaling Technology ...
• Poly(allylamine)-tripolyphosphate polymeric nanoparticle as an NLRP3-dependent systemic adjuvant for the vaccine development

  Gastón P. Rizzo, et al., bioRxiv - Immunology 2024

  Quote: ... mice with 20% L929-conditioned medium (LCCM) in DMEM and 20ng/mL recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) (R&D Systems, Minneapolis, MN, USA) in RPMI for BMDM and BMDC ...
• FABP4-mediated lipid accumulation and lipolysis in tumor associated macrophages promote breast cancer metastasis

  Matthew Yorek, et al., bioRxiv - Immunology 2024

  Quote: ... Unconjugated antibodies were used for quantification of intracellular markers: m/rFABP4 (R&D Systems), GPAM (Invitrogen) ...
• Poly(allylamine)-tripolyphosphate polymeric nanoparticle as an NLRP3-dependent systemic adjuvant for the vaccine development

  Gastón P. Rizzo, et al., bioRxiv - Immunology 2024

  Quote: ... or with 50ng/mL TNF-α (R&D Systems, Minneapolis, USA) for 30min at 37°C followed with FITC-PAH/Tpp Np ...
• Indels allow antiviral proteins to evolve functional novelty inaccessible by missense mutations

  Jeannette L. Tenthorey, et al., bioRxiv - Evolutionary Biology 2024

  Quote: ... Cell supernatants were tested for mycoplasma contamination by MycoProbe Kit (R&D Systems) or MycoStrip (Invi-voGen) ...
• High IL-1β and IL-18 Levels Associate with Gut Barrier Disruption and Monocyte Activation During Chronic SIV Infection with Long-Term Suppressive Antiretroviral Therapy

  Siva Thirugnanam, et al., bioRxiv - Immunology 2024

  Quote: Caspase-1 levels in macaque serum samples were quantitatively determined using the Quantikine™ ELISA Human Caspase-1/ICE Immunoassay (Catalog# DCA100, R&D Systems, Minneapolis, MN). The assay was conducted following the manufacturer’s instructions ...
• A selective alternative pathway complement inhibitor for treatment of paroxysmal nocturnal hemoglobinuria

  Hagen Sülzen, et al., bioRxiv - Immunology 2024

  Quote: ... RBCs were washed twice with ice cold RBC staining buffer (PBS supplemented with 0.2% BSA) before staining on ice with anti-CD59AF405 monoclonal antibody (FAB1987V, R&D Systems, 5 ng/µL final concentration) and anti-C3c-FITC polyclonal antibody (ab4212 ...
• B lymphocytes acquire myeloid and autoimmune phenotypes via the downregulation of lymphocyte-specific protein-1

  Naeun Lee, et al., bioRxiv - Immunology 2024

  Quote: ... MPO (R&D Systems; #AF3667); Blimp1 (Santa Cruz Biotechnology ...
• Effector T cells in poorly perfused tumor regions exhibit a distinct signature of augmented IFN response and reduced PD-1 expression

  Marta Riera-Borrull, et al., bioRxiv - Immunology 2024

  Quote: ... for 24 hours without or with additional stimulation with a cocktail of 600 U/ml IFNα4 (recombinant mouse IFN alpha 4, R&D Systems, catalog: 12115-1) and 2.5 ng/ml IFNβ1 (recombinant mouse IFN-β1 (carrier-free) ...
• ACKR5/GPR182 is a scavenger receptor for the atypical chemokine CXCL17, GPR15L and various endogenous peptides

  Max Meyrath, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... MIF and osteopontin from R&D Systems, 5-oxo-eicosatetraenoic acid and prostaglandin from Santa Cruz ...
• ACKR5/GPR182 is a scavenger receptor for the atypical chemokine CXCL17, GPR15L and various endogenous peptides

  Max Meyrath, et al., bioRxiv - Pharmacology and Toxicology 2024

  Quote: ... mCXCL7 from R&D Systems, mCXCL3 mXCL1 ...