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• Lactational delivery of Triclosan promotes non-alcoholic fatty liver disease in newborn mice

  André A. Weber, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: FGF21 and triglyceride serum levels were measured using FGF-21 Quantikine enzyme-linked immunosorbent assay kit (R&D System), triglyceride colorimetric assay kit (Cayman Chemicals) ...
• GDF15 and ACE2 stratify COVID19 patients according to severity while ACE2 mutations increase infection susceptibility

  Margalida Torrens-Mas, et al., bioRxiv - Molecular Biology 2022

  Quote: GDF15 circulating levels were measured using the Human GDF15 Quantikine® ELISA Kit (#DGD150, R&D Systems). Plasma samples were diluted 1:4 with the Calibrator Diluent and plated and incubated overnight at 4 °C with gentle rocking ...
• An electro-optical bead-nanochip technology for the ultrasensitive and multi-dimensional detection of small extracellular vesicles and their markers

  Tomás Dias, et al., bioRxiv - Molecular Biology 2022

  Quote: ... or anti-HER2 (FAB9589B-100, R&D Systems) were added to each sEV sample totalling a final total volume of 200 µL per well in a U-shaped 96-well plate and incubated for 45 minutes at RT and 200 RPM ...
• An electro-optical bead-nanochip technology for the ultrasensitive and multi-dimensional detection of small extracellular vesicles and their markers

  Tomás Dias, et al., bioRxiv - Molecular Biology 2022

  Quote: ... The secondary antibody used was a secondary antibody anti-mouse (HAF007, R&D Systems) at 1:250 dilution.
• A multiple comorbidities mouse model to assess atherosclerosis progression following lung infection in ApoE deficient mice

  Benjamin Bartlett, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Dickkopf (Dkk)-1 and matrix metalloproteinase (MMP)-12 were assayed using the multiplex Mouse Magnetic Luminex Assay (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions ...
• Less is more - loss of EGFL7 improves memory by upregulation of VEGF-D

  Verica Vasić, et al., bioRxiv - Neuroscience 2022

  Quote: ... The pump delivered 1 µg/d of recombinant purified VEGF-D (R&D systems, Minneapolis, MN, USA) into the ventricle for 7 d at 1 µl/h ...
• Monitoring regional astrocyte diversity by cell-type specific proteomic labeling in vivo

  Priyadharshini Prabhakar, et al., bioRxiv - Neuroscience 2022

  Quote: ... Anti-SparcL1(Sparc-Like1; R&D systems, Minneapolis, USA; AF2836), Anti-Gys1 (Glycogen Synthase1 ...
• Evaluation of Blood-Based Exosomes as Biomarkers for Aging-Related TDP-43 pathology

  Charisse N. Winston, et al., bioRxiv - Neuroscience 2022

  Quote: ... washed 3× in 1× wash buffer (895003, R&D Systems), blocked in 1% BSA for 1 hour at room temperature (RT) ...
• Evaluation of Blood-Based Exosomes as Biomarkers for Aging-Related TDP-43 pathology

  Charisse N. Winston, et al., bioRxiv - Neuroscience 2022

  Quote: ... Human TDP-43 DuoSET ELISA kit DuoSet Ancillary Reagent Kit 2 (#DY008, R&D Systems, Minneapolis, MN, USA), and tetraspanin exosome marker CD81 (CSB-EL004960HU ...
• Pleiotrophin signals through ALK receptor to enhance growth of neurons in the presence of inhibitory CSPGs

  Somnath J Gupta, et al., bioRxiv - Neuroscience 2022

  Quote: ... the media was incubated (72 h) with media containing different concentration of recombinant human PTN (rhPTN, R&D systems 252 - PL) or the drugs alectinib (Toronto Research Chemicals ...
• Pin1-promoted SUMOylation of RNF168 restrains its chromatin accumulation

  Anoop Singh Chauhan, et al., bioRxiv - Molecular Biology 2022

  Quote: ... mouse anti Pin1 mAb (1:2000 dilution, R&D Systems, MAB2294), rabbit anti Histone H3 polyclonal Ab (1:2000 dilution ...
• APOE modulates microglial immunometabolism in response to age, amyloid pathology, and inflammatory challenge

  Sangderk Lee, et al., bioRxiv - Neuroscience 2022

  Quote: ... cells were stimulated with a pro-inflammatory cocktail of 20ng/ml interferon-γ (IFNγ, R&D Systems #485-MI-100) and 50ng/ml tumor necrosis factor α (TNFα ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-mouse IgM 405 (Alexa Fluor R&D systems), anti-mouse IgG1 647 (Alexa Fluor R&D systems ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-rabbit 647 (Alexa Fluor R&D systems), anti-mouse IgM 405 (Alexa Fluor R&D systems) ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-mouse IgG1 647 (Alexa Fluor R&D systems, anti-rat 555 (Alexa Fluor R&D systems) and DAPI (Sigma ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-mouse IgG1 647 (Alexa Fluor R&D systems, anti-rat 555 (Alexa Fluor R&D systems) and DAPI (Sigma ...
• Pathogenic TDP-43 Disrupts Axon Initial Segment Structure and Neuronal Excitability in a Human iPSC Model of ALS

  Peter Harley, et al., bioRxiv - Neuroscience 2022

  Quote: ... Cells were then washed 3x in 0.1% Triton X-100 in PBS and incubated with the secondary antibodies: anti-mouse IgG2a 647 (Alexa Fluor R&D systems), anti-mouse IgG2a 488 (Alexa Fluor R&D systems) ...
• Biocompatible polymers for scalable production of human neural organoids

  Genta Narazaki, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 20 ng/mL FGF2 (R&D Systems, cat. no. 233-FB). From day 15 of differentiation ...
• Biocompatible polymers for scalable production of human neural organoids

  Genta Narazaki, et al., bioRxiv - Neuroscience 2022

  Quote: ... and SB-431542 (10 μM, R&D Systems, 1614). On day 6 in suspension the organoids were transferred to neural medium containing Neurobasal™-A Medium (Thermo Fisher Scientific ...
• Disruption of Smarce1, a component of the SWI/SNF chromatin remodeling complex, decreases nucleosome stability in mouse embryonic stem cells and impairs differentiation

  Katsunobu Kashiwagi, et al., bioRxiv - Molecular Biology 2022

  Quote: ... anti-β-III tubulin (1:125; R&D Systems, MAB1195), anti-α smooth muscle actin (1:250 ...
• Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks

  Matthew W. Kenaston, et al., bioRxiv - Molecular Biology 2022

  Quote: ... and IFN-β (50 U/mL, R&D Systems) at the given concentrations ...
• Transcriptomic profiling implicates PAF1 in both active and repressive immune regulatory networks

  Matthew W. Kenaston, et al., bioRxiv - Molecular Biology 2022

  Quote: ... IFN-β (50 U/mL, R&D Systems), and TNF-α (100 ng/mL ...
• A transcriptional mechanism involving R-loop, m6A modification and RNA abasic sites regulates an enhancer RNA of APOE

  Jason A. Watts, et al., bioRxiv - Molecular Biology 2022

  Quote: ... iPSC at 60-70% confluence were treated for 3 days with 100 ng/mL Activin A and 100 ng/mL bFGF (R&D Systems) in the presence of increasing levels of FBS (0% on day 1 ...
• High-efficiency HDR in immortalized cell lines by crude rAAV mediated donor template delivery

  Stuti Mehta, et al., bioRxiv - Molecular Biology 2022

  Quote: ... 50 ng/mL recombinant human stem cell factor (R&D systems), 3 IU/mL Epoetin-alfa (Amgen) ...
• The ADAM17 sheddase complex regulator iTAP modulates inflammation, epithelial repair, and tumor growth

  Marina Badenes, et al., bioRxiv - Molecular Biology 2022

  Quote: ... HB-EGF and Amphiregulin using specific ELISA kits (88-7324-22 eBioscience, DY8239-05, R&D Systems, DY989, R&D Systems, respectively).
• The ADAM17 sheddase complex regulator iTAP modulates inflammation, epithelial repair, and tumor growth

  Marina Badenes, et al., bioRxiv - Molecular Biology 2022

  Quote: ... HB-EGF and Amphiregulin using specific ELISA kits (88-7324-22 eBioscience, DY8239-05, R&D Systems, DY989, R&D Systems, respectively).
• GDF15 and ACE2 stratify COVID19 patients according to severity while ACE2 mutations increase infection susceptibility

  Margalida Torrens-Mas, et al., bioRxiv - Molecular Biology 2022

  Quote: ... or anti-ACE2 (1:50; MAB933; R&D Systems) in PBST containing 0,1% BSA ON at 4°C ...
• Induced pluripotent stem cell-derived astrocytes from patients with schizophrenia exhibit an inflammatory phenotype that affects vascularization

  Pablo Trindade, et al., bioRxiv - Neuroscience 2022

  Quote: ... Human recombinant IL-8 (catalog no. 208-IL-010, R&D Systems, USA) was used at a 5 ng/ml concentration ...
• Microglial TNFα orchestrates brain phosphorylation during the sleep period and controls homeostatic sleep

  Maria J Pinto, et al., bioRxiv - Neuroscience 2022

  Quote: Stimulation of organotypic slices with TNFα neutralizing antibody (1 μg/ml, R&D systems, MAB4101) was performed at DIV18-21 in pre-warmed aCSF (aCSF ...
• Differentiation of airway cholinergic neurons from human pluripotent stem cells for airway neurobiology studies

  P.A. Goldsteen, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 2 µg/mL laminin (LM, R&D systems, 3400-010-02).
• Marked regional glial heterogeneity in the human white matter of the central nervous system

  Luise A. Seeker, et al., bioRxiv - Neuroscience 2022

  Quote: ... and the Olig2 (R&D Systems, AF2418) antibodies were used at a 1:100 dilution.
• Microglial Piezo1 senses Aβ fibrils stiffness to restrict Alzheimer’s disease

  Jin Hu, et al., bioRxiv - Neuroscience 2022

  Quote: ... The Human Aβ1-42 in soluble and insoluble fractions were qualified with Quantikine ™ ELISA kit (R&D Systems) based on the manufacturer’s instructions.
• SARS-CoV-2 spike proteins uptake mediated by lipid raft ganglioside GM1 in human cerebrovascular cells

  Conor McQuaid, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti ACE2 antibody (R&D Systems, Cat# AF933) used at a low (10 μg/m)l and high (60 μg/ml ...
• SARS-CoV-2 spike proteins uptake mediated by lipid raft ganglioside GM1 in human cerebrovascular cells

  Conor McQuaid, et al., bioRxiv - Neuroscience 2022

  Quote: ... Anti-ACE2 antibody (R&D Systems, Cat# AF933) was labeled with Alexa Fluor 488 by following the manufacturer instructions (Microscale protein labeling kit ...
• The role of UBE3A in the autism and epilepsy-related Dup15q syndrome using patient-derived, CRISPR-corrected neurons

  Marwa Elamin, et al., bioRxiv - Neuroscience 2022

  Quote: ... 500ng/mL μM of Noggin (R&D Systems, Minneapolis MN) and 2 μM SB431542 (Stemgent ...
• Carbohydrate-Binding Protein from Stinging Nettle as Fusion Inhibitor for SARS-CoV-2 Variants of Concern

  Emiel Vanhulle, et al., bioRxiv - Microbiology 2022

  Quote: The following antibodies were used for western blotting: ACE2 Polyclonal Goat IgG (cat. n° AF933, R&D systems), anti-β-actin (cat ...
• Targeting the BAG-1 family of co-chaperones in lethal prostate cancer

  Antje Neeb, et al., bioRxiv - Molecular Biology 2022

  Quote: ... panBAG-1 (mouse, goat polyclonal, AF815, R&D systems), and AR-FL (mouse/human ...
• Recombinant Human Proteoglycan 4 (rhPRG4) Downregulates TNFα-Stimulated NFκB Activity and FAT10 Expression in Human Corneal Epithelial Cells

  Nikhil G Menon, et al., bioRxiv - Molecular Biology 2022

  Quote: ... + 10% FBS (R&D Systems, Minneapolis, MN) + 10 ng/mL EGF (Gibco).
• Transcription factors interact with RNA to regulate genes

  Ozgur Oksuz, et al., bioRxiv - Molecular Biology 2022

  Quote: ... and Klf4 (R&D Systems, AF3158) with secondary antibody anti-mouse for Sox2 (IRDye 680CW goat anti-mouse LI-COR 926-32211 ...
• Excessive fluid shear stress-mediated Klf4 leads to arteriovenous pathogenesis

  Kuheli Banerjee, et al., bioRxiv - Molecular Biology 2022

  Quote: ... anti-KLF4 (#AF3158, 1:200, R&D systems), anti-phospho S6 (pS6 #5364 ...
• Transcription factors interact with RNA to regulate genes

  Ozgur Oksuz, et al., bioRxiv - Molecular Biology 2022

  Quote: ... antibody for Sox2 (R&D Systems, MAB2018) and Klf4 (R&D Systems ...
• Nhlh1 and Nhlh2, a global transcriptional mechanism regulating commissural axon projection via Robo3 activation

  Aki Masuda, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Robo3 polyclonal antibody (R&D Systems, AF3076, 1:200), goat anti-Tag1 polyclonal antibody (R&D Systems ...
• Nhlh1 and Nhlh2, a global transcriptional mechanism regulating commissural axon projection via Robo3 activation

  Aki Masuda, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Tag1 polyclonal antibody (R&D Systems, AF4439, 1:500), mouse anti-Neurofilament-160KD monoclonal antibody (clone ...
• The crosstalk between microbial sensors ELMO1 and NOD2 shape intestinal immune responses

  Aditi Sharma, et al., bioRxiv - Microbiology 2022

  Quote: ... and MCP1 using the ELISA kit from R&D systems as per manufacturer’s protocol.
• Epistasis reduces fitness costs of influenza A virus escape from stem-binding antibodies

  Chung-Young Lee, et al., bioRxiv - Microbiology 2022

  Quote: ... supplemented with 10% fetal bovine serum (FBS) (R&D SYSTEMS, USA) and penicillin-streptomycin (PS ...
• Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer’s Disease

  Michael J. D. Daniels, et al., bioRxiv - Neuroscience 2022

  Quote: ... cells were lysed for RNA analysis and supernatants were analysed for IL-6 content by ELISA (DuoSet, R&D Systems) according to manufacturer’s instructions.
• Cystatin F (Cst7) drives sex-dependent changes in microglia in an amyloid-driven model of Alzheimer’s Disease

  Michael J. D. Daniels, et al., bioRxiv - Neuroscience 2022

  Quote: ... IL-4 (20 ng/mL, 24 h, R&D Systems) or apoptotic SH-SY5Y neuroblastoma cells (2:1 ratio neurons:microglia ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... and PRELP (R&D Systems Cat#6447-PR)) ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... sheep polyclonal anti-PRELP (R&D Systems Cat#AF6447), rabbit polyclonal anti-CHAD (Invitrogen Cat#PA-553761) ...
• Small leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment

  Julia Kolb, et al., bioRxiv - Neuroscience 2022

  Quote: ... FMOD (R&D Systems Cat#9840-FM), LUM (Abcam Cat#ab221400) ...