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• Parvalbumin protein controls inhibitory tone in the spinal cord

  Haoyi Qiu, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Pax2 goat (1:1000, R&D Systems #AF3364), anti-Tlx3 rabbit (1:1000 ...
• X-linked adrenoleukodystrophy patient fibroblast-iPSC-derived astrocytes reveal phenotype-specific metabolic, inflammatory, and microRNA alterations

  Parveen Parasar, et al., bioRxiv - Neuroscience 2022

  Quote: ... basic fibroblast growth factor (8 ng/mL, R&D Systems), penicillin-streptomycin (1X ...
• X-linked adrenoleukodystrophy patient fibroblast-iPSC-derived astrocytes reveal phenotype-specific metabolic, inflammatory, and microRNA alterations

  Parveen Parasar, et al., bioRxiv - Neuroscience 2022

  Quote: ... containing N-21 max (1X, R&D Systems), fetal bovine serum One Shot (1X ...
• CNS myelination requires VAMP2/3-mediated membrane expansion in oligodendrocytes

  Mable Lam, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 1x NS21-MAX (R&D Systems AR008).
• Prox2+ and Runx3+ neurons regulate esophageal motility

  Elijah D. Lowenstein, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-CD117 (R&D Systems, AF1356, 1:400), sheep anti-nNos (Millipore ...
• Prox2+ and Runx3+ neurons regulate esophageal motility

  Elijah D. Lowenstein, et al., bioRxiv - Neuroscience 2022

  Quote: ... The following primary antibodies were used: goat anti-Phox2b (R&D Systems, AF4940, 1:200), rabbit anti-RFP (Rockland ...
• Systematic comparison of culture media uncovers phenotypic shift of human microglia defined by reduced reliance to CSF1R signaling

  Marie-France Dorion, et al., bioRxiv - Neuroscience 2022

  Quote: ... The membrane was immunoblotted for CSF1R (#MAB3291, R&D Systems at 1:250) and GAPDH (#G8795 ...
• Dynamics and mechanisms of ERK activation after different protocols that induce long-term facilitation in Aplysia

  Yili Zhang, et al., bioRxiv - Neuroscience 2022

  Quote: ... 5 μg/ml of TGF-β RII Fc chimera (TGF-β RII Fc) (R&D Systems), was applied to SN cultures 1 h prior to fixation to inhibit TGF-β signaling ...
• Production of brain-derived neurotrophic factor gates plasticity in developing visual cortex

  Megumi Kaneko, Michael P. Stryker, bioRxiv - Neuroscience 2022

  Quote: ... The cannula was connected to an osmotic minipump filled either with vehicle solution (PBS containing 0.1% BSA as a carrier) or 35 μg/ml of soluble TNF receptor-1 (sTNFR1, R&D Systems Inc. Minneapolis, MN).
• Mutation in the TRKB cholesterol recognition site that blocks antidepressant binding does not influence the basal or BDNF-stimulated activation of TRKB

  Caroline Biojone, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-TRKB (R&D System, #AF1494) or anti-β-actin mouse monoclonal antibody (Sigma-Aldrich ...
• Mutation in the TRKB cholesterol recognition site that blocks antidepressant binding does not influence the basal or BDNF-stimulated activation of TRKB

  Caroline Biojone, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-TRKB antibody (R&D System, #AF1494) was diluted 1:500 in carbonate buffer pH 9.7 (25 mM sodium bicarbonate ...
• Microcephaly-associated WDR62 mutations hamper Golgi apparatus-to-spindle pole shuttling in human neural progenitors

  Claudia Dell’Amico, et al., bioRxiv - Neuroscience 2022

  Quote: ... BDNF (30 ng/ml, R&D Systems, #248-DB025), and Y-27632 (10 μM ...
• Binucleated cells generated by nuclear movement during neural transdifferentiation from human bone marrow-derived mesenchymal cells

  Carlos Bueno, et al., bioRxiv - Neuroscience 2022

  Quote: ... supplemented with N2-supplement (R&D systems), 0.6% glucose (Sigma-Aldrich) ...
• Binucleated cells generated by nuclear movement during neural transdifferentiation from human bone marrow-derived mesenchymal cells

  Carlos Bueno, et al., bioRxiv - Neuroscience 2022

  Quote: ... and IGF-1 (10 ng/ml; R&D systems). Time-lapse analysis was carried out using a Widefield Leica Thunder-TIRF imager microscope ...
• Patterns of NFkB activation resulting from damage, reactive microglia, cytokines, and growth factors in the mouse retina

  Isabella Palazzo, et al., bioRxiv - Neuroscience 2022

  Quote: ... BIO (500 ng/dose; R&D Systems; 3194), CHIR 99021 (500 ng/dose ...
• Patterns of NFkB activation resulting from damage, reactive microglia, cytokines, and growth factors in the mouse retina

  Isabella Palazzo, et al., bioRxiv - Neuroscience 2022

  Quote: ... CHIR 99021 (500 ng/dose; R&D Systems; 4423).
• Patterns of NFkB activation resulting from damage, reactive microglia, cytokines, and growth factors in the mouse retina

  Isabella Palazzo, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF (300ng/dose; R&D systems; 557-NT), FGF2 (250 ng/dose ...
• Patterns of NFkB activation resulting from damage, reactive microglia, cytokines, and growth factors in the mouse retina

  Isabella Palazzo, et al., bioRxiv - Neuroscience 2022

  Quote: ... FGF2 (250 ng/dose; R&D systems; 233-FB), Dexamethasone (Dex ...
• Patterns of NFkB activation resulting from damage, reactive microglia, cytokines, and growth factors in the mouse retina

  Isabella Palazzo, et al., bioRxiv - Neuroscience 2022

  Quote: ... recombinant mouse IL1β (200 ng/dose; R&D systems; 401-ML) recombinant mouse TNF (250 ng/dose ...
• A branching model of cell fate decisions in the enteric nervous system

  Anna Laddach, et al., bioRxiv - Neuroscience 2022

  Quote: ... NT3 (10 ng/ml, R&D systems, 267-N3), NGF-7S (10 ng/ml) ...
• A branching model of cell fate decisions in the enteric nervous system

  Anna Laddach, et al., bioRxiv - Neuroscience 2022

  Quote: ... BDNF (10 ng/ml, R&D systems, 248-BD), NT3 (10 ng/ml ...
• Neuroinvasion and anosmia are independent phenomena upon infection with SARS-CoV-2 and its variants

  Guilherme Dias de Melo, et al., bioRxiv - Neuroscience 2022

  Quote: ... Goat anti-CD31 (AF3628, R&D Systems) diluted 1:300 ...
• Whole-body sorcin invalidation does not cause hypothalamic ER stress nor worsens obesity in C57BL/6 male mice fed a westernized diet

  Steven Z. Parks, Guy A. Rutter, Isabelle Leclerc, bioRxiv - Neuroscience 2022

  Quote: ... Cells were left for 48h to allow expression of LepRb-HA protein before treatment with recombinant murine leptin (R&D System #498-OB) at 0-10 ng/L for 30 minutes before cell lysis and western blotting for phosphoSTAT3 (Y705) ...
• Whole-body sorcin invalidation does not cause hypothalamic ER stress nor worsens obesity in C57BL/6 male mice fed a westernized diet

  Steven Z. Parks, Guy A. Rutter, Isabelle Leclerc, bioRxiv - Neuroscience 2022

  Quote: ... Mice were then fasted for 16h overnight before intraperitoneal administration of recombinant mouse leptin (3μg/g) (R&D System) to half of the cohort and vehicle control (sterile Tris-HCl 20 mM ...
• Integrative Multiomic Analyses of Diabetic Neuropathic Pain in Dorsal Root Ganglia: Proteomics, Phospho-proteomics, and Metabolomics

  Megan Doty, et al., bioRxiv - Neuroscience 2022

  Quote: ... TNR (1/2000, AF3865, R&D systems), alpha-tubulin (1/10,000 ...
• Integrative Multiomic Analyses of Diabetic Neuropathic Pain in Dorsal Root Ganglia: Proteomics, Phospho-proteomics, and Metabolomics

  Megan Doty, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were stained using a primary antibody raised against TNR (1/50, AF3865, R&D systems) and secondary antibody conjugated to Rhodamine Red-X raised against goat IgG (1/100 ...
• Changes in adenosine and neurotrophic signaling in the SOD1^G93A mouse model of amyotrophic lateral sclerosis: modulation by chronic caffeine intake

  Nádia Rei, et al., bioRxiv - Neuroscience 2022

  Quote: ... VEGFR-1 and VEGFR-2 protein levels were quantified with the DuoSet® ELISA Development System Kit (R&D Systems, Abingdon, UK), according to manufacturer instructions ...
• Expression bias in retinoic acid responsive genes defines variations in neural differentiation of human pluripotent stem cells

  Suel-Kee Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... human BDNF (10 ng/ml, 248-BD, R&D Systems) and human NT-3 (10 ng/ml ...
• Expression bias in retinoic acid responsive genes defines variations in neural differentiation of human pluripotent stem cells

  Suel-Kee Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... Noggin (500 ng/ml, 719-NG, R&D Systems) and SB431542 (2 μM ...
• Expression bias in retinoic acid responsive genes defines variations in neural differentiation of human pluripotent stem cells

  Suel-Kee Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... and human NT-3 (10 ng/ml, 267-N3, R&D Systems) for another 20 days.
• Nuclear lamina strain states revealed by intermolecular force biosensor

  Brooke E. Danielsson, et al., bioRxiv - Biophysics 2022

  Quote: ... recombinant human TGF-β1 (R&D systems) was used to induce EMT at a concentration of 2 ng/mL for 24 h ...
• Expression bias in retinoic acid responsive genes defines variations in neural differentiation of human pluripotent stem cells

  Suel-Kee Kim, et al., bioRxiv - Neuroscience 2022

  Quote: ... while BMP4 (100 ng/ml, 314-BP, R&D Systems) was added for mesendodermal differentiation upon ROCK inhibitor removal (Day 0 ...
• CiDRE⁺ M2c macrophages hijacked by SARS-CoV-2 cause COVID-19 severity

  Yuichi Mitsui, et al., bioRxiv - Immunology 2022

  Quote: ... goat anti-ACE2 antibody (R&D Systems) and Alexa Flour 647-conjugated anti-goat IgG antibody (Abcam ...
• CiDRE⁺ M2c macrophages hijacked by SARS-CoV-2 cause COVID-19 severity

  Yuichi Mitsui, et al., bioRxiv - Immunology 2022

  Quote: ... USA) and cultured for 7 days with 20 ng/ml recombinant M-CSF (R&D Systems) to allow them to differentiate to macrophages ...
• CiDRE⁺ M2c macrophages hijacked by SARS-CoV-2 cause COVID-19 severity

  Yuichi Mitsui, et al., bioRxiv - Immunology 2022

  Quote: ... and MRP-8/14 in human blood serum were measured by ELISA kits (BioLegend, R&D Systems, or Shino-Test Corp., Tokyo, Japan) following the manufacturer’s protocols.
• CiDRE⁺ M2c macrophages hijacked by SARS-CoV-2 cause COVID-19 severity

  Yuichi Mitsui, et al., bioRxiv - Immunology 2022

  Quote: ... or IL-10 with or without anti-IL-10RB antibody (clone 90220, R&D Systems, Minneapolis, MN, USA) for 30 min ...
• Systematic functional screening of chromatin factors identifies strong lineage and disease dependencies in normal and malignant haematopoiesis

  D Lara-Astiaso, et al., bioRxiv - Genomics 2022

  Quote: ... and murine IL-6 10 ng/mL (R&D Systems #406-ML-025), in a 37°C and 5% CO2 atmospheric environment.
• CLIP-Seq Analysis Enables the Design of Ribosomal RNA Bait Oligonucleotides That Protect Against C9ORF72 ALS/FTD-Associated Poly-GR Pathophysiology

  Juan A. Ortega, et al., bioRxiv - Neuroscience 2022

  Quote: ... CNTF and GDNF (10ng/mL, R&D systems).
• A newly-recognized population of residual neural crest cells in the adult leptomeninges is re-activated for vascular repair

  Yoshihiko Nakamura, et al., bioRxiv - Neuroscience 2022

  Quote: ... recombinant human Pleiotrophin (252-PL-050, R&D systems), pAAV-CAG-tdTomato (#59462-AAV5 ...
• A newly-recognized population of residual neural crest cells in the adult leptomeninges is re-activated for vascular repair

  Yoshihiko Nakamura, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Bmp7 antibody (1:100, AF354, R&D Systems), anti-Col3a1 antibody (1:100 ...
• Contribution of Notch/Wnt signaling modulation in reactive astrocyte reparative response after brain injury

  Lina Maria Delgado-Garcia, et al., bioRxiv - Neuroscience 2022

  Quote: ... Recombinant mouse Wnt-3a (rmWnt) (1324-WN-010, R&D system, CA, USA) was diluted in PBS and 0,1% BSA to a stock concentration of 40 µg/mL ...
• Contribution of Notch/Wnt signaling modulation in reactive astrocyte reparative response after brain injury

  Lina Maria Delgado-Garcia, et al., bioRxiv - Neuroscience 2022

  Quote: ... N-Terminus (rmShh) (464-SH-025, R&D system, CA, USA) was diluted in PBS 1x and 0,1% BSA to a stock concentration of 125 µg/mL ...
• Extracellular vesicle-associated cholesterol dictates the regenerative functions of macrophages in the brain

  Sam Vanherle, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-O4 (1:1000, cat. #MAB1326, R&D systems), anti-Olig2 (1:100 ...
• Unique functional responses differentially map onto genetic subtypes of dopamine neurons

  Maite Azcorra, et al., bioRxiv - Neuroscience 2022

  Quote: ... Sections were incubated with primary antibodies against Aldh1a1 (goat, R&D Systems Cat# AF5869, RRID:AB_2044597), TH (mouse ...
• Membrane association is required for Stmn2-mediated axon protection

  Emma J.C. Thornburg-Suresh, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Stmn2 (R&D Systems; RRID:AB_10972937; 1:1,000), anti-Stmn3 (Proteintech ...
• GDF15 Is Required for Cold-Induced Thermogenesis and Contributes to Improve Systemic Metabolic Health Following Loss of OPA1 in Brown Adipocytes

  Jayashree Jena, et al., bioRxiv - Physiology 2022

  Quote: ... Serum GDF15 was measured using commercially available kits according to the manufacturers’ directions (R&D Systems, Minneapolis, MN, USA). Body composition was determined by nuclear magnetic resonance (NMR ...
• Extracellular vesicle-associated cholesterol dictates the regenerative functions of macrophages in the brain

  Sam Vanherle, et al., bioRxiv - Neuroscience 2022

  Quote: ... anti-Olig2 (1:100, cat. #AF2418, R&D systems), anti-CC1 (1:100 ...
• Machine-learning dissection of Human Accelerated Regions in primate neurodevelopment

  Sean Whalen, et al., bioRxiv - Evolutionary Biology 2022

  Quote: ... supplemented with 100 ng/ml mouse recombinant Noggin (R&D systems). N1 cells were collected eleven days after initiating neural induction ...
• Japanese Encephalitis Virus NS1’ Protein regulates the expression and distribution of TIM-1 to promote viral infection

  Zhenjie Liang, et al., bioRxiv - Microbiology 2022

  Quote: ... Anti-Human TIM-1 monoclonal antibody (MAB1750) and polyclonal antibody (AF1750) were purchased from R&D systems (Minnesota, USA). Anti-GAPDH polyclonal antibody (sc-25778 ...
• Myofibroblast Ccn3 is regulated by Yap and Wwtr1 and contributes to adverse cardiac outcomes

  Michael A. Flinn, et al., bioRxiv - Physiology 2022

  Quote: ... recombinant CCN3 (R&D Systems 1640-NV-050) was resuspended in PBS at a concentration of 0.01 µg/µL ...