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• Astrocytic synapse elimination controls ocular dominance plasticity

  Joon-Hyuk Lee, et al., bioRxiv - Neuroscience 2022

  Quote: ... mouse anti-AXL (R&D systems), rat anti-MERTK (eBioscience).
• Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model

  Volha Liaudanskaya, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 100 ng/mL OX-2 membrane glycoprotein (CD200) (R&D systems, cat.no. 27712CD050) growth factors in addition to the previously described growth factors.
• Natural and pathological aging distinctively impact the vomeronasal detection system and social behavior

  Adrián Portalés, Pablo Chamero, Sandra Jurado, bioRxiv - Neuroscience 2022

  Quote: ... Sox2 (R&D Systems, goat polyclonal; 1:300). For PCNA immunostaining ...
• Astrocytic synapse elimination controls ocular dominance plasticity

  Joon-Hyuk Lee, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-Cathepsin D (R&D systems), guinea pig anti-VGAT (Synaptic systems) ...
• Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model

  Volha Liaudanskaya, et al., bioRxiv - Neuroscience 2022

  Quote: ... 100µL of collagen type I solution (Corning or R&D systems, 3 mg/mL with a pH adjusted to 7.0-7.2 with NaOH ...
• Mitochondria dysregulation contributes to secondary neurodegeneration progression post-contusion injury in human 3D in vitro triculture brain tissue model

  Volha Liaudanskaya, et al., bioRxiv - Neuroscience 2022

  Quote: ... Then 1 ml of fresh media was supplemented with IL-34 (R&D systems, cat. No. 5265-IL-010), MCSF (Invitrogen ...
• NOTCH3 active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model

  Daniel V. Oliveira, et al., bioRxiv - Neuroscience 2022

  Quote: ... Pre-warmed sulfuric acid stop solution (DY994, R&D Systems) at 37 °C was used to stop the reaction and absorbance at 450 nm was measured in a Fluorostar galaxy plate reader.
• Enteric glia adopt an activated pro-inflammatory state in response to human and bacterial amyloids

  Peter Verstraelen, et al., bioRxiv - Neuroscience 2022

  Quote: ... 40 ng/ml GDNF (R&D Systems 512-GF-010), 1% glutamax ...
• Metabolic differences and differentially expressed genes between C57BL/6J and C57BL/6N mice substrains

  Shino Nemoto, Tetsuya Kubota, bioRxiv - Genetics 2022

  Quote: ... leptin (R&D Systems, Minneapolis, MN, USA), and adiponectin (Otsuka Pharmaceutical Co. ...
• Embryonic cortical layer 5 pyramidal neurons form an active, transient circuit motif perturbed by autism-associated mutations

  Martin Munz, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat polyclonal anti Nurr1/NGFI-Bbeta/NR4A2 (AF2156, R&D Systems), rat monoclonal anti CTIP2/BCL11B (MABE1045 ...
• Regulation of neuropathic pain by microglial Orai1 channels

  Shogo Tsujikawa, et al., bioRxiv - Neuroscience 2022

  Quote: ... Supernatants were subsequently analyzed using mouse ELISA kits for the release of TNFα (R&D Systems, MTA00B), IL-6 (RayBiotech ...
• LRRK2-G2019S Synergizes with Ageing and Low-Grade Inflammation to Promote Gut and Peripheral Immune Cell Activation that Precede Nigrostriatal Degeneration

  Carmela Giachino, et al., bioRxiv - Neuroscience 2022

  Quote: ... TNF-α (MTA00B) and IL-6 (M6000B) levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (ELISA Development System; R&D Systems, McKinley Place, MN, USA) following the manufacturer’s protocol.75
• Myelin plasticity in ventral tegmental area is required for opioid reward

  Belgin Yalçın, et al., bioRxiv - Neuroscience 2022

  Quote: ... N21-MAX (R&D systems, AR012), trace elements B (Corning ...
• Astrocytic TDP-43 dysregulation impairs memory by modulating antiviral pathways and interferon-inducible chemokines

  Avital Licht-Murava, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-CXCL10 (1:150; R&D Systems #AF-466-NA; RRID # AB_2292487), rabbit anti-GFAP (1:1000 ...
• CRISPR screens in 3D assembloids reveal disease genes associated with human interneuron development

  Xiangling Meng, et al., bioRxiv - Neuroscience 2022

  Quote: ... SB-431542 (10 μM, R&D Systems, 1614), and XAV-939 (1.25 μM ...
• CRISPR screens in 3D assembloids reveal disease genes associated with human interneuron development

  Xiangling Meng, et al., bioRxiv - Neuroscience 2022

  Quote: ... Neural medium was supplemented with 20 ng/ml EGF (R&D Systems) and 20 ng/mL FGF2 (R&D Systems ...
• CRISPR screens in 3D assembloids reveal disease genes associated with human interneuron development

  Xiangling Meng, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 20 ng/mL FGF2 (R&D Systems) from day 6 to day 24 with daily medium change in the first 10 days ...
• Neurodevelopmental disorder associated mutations in TAOK1 reveal its function as a plasma membrane remodeling kinase

  Neal Beeman, et al., bioRxiv - Neuroscience 2022

  Quote: ... Samples were then subjected to western blot analysis detailed above to detect TAOK2 autophosphorylation by rabbit antibody against pS181 (R&D Systems, PPS037).
• Rejection of inappropriate synaptic partners mediated by transcellular FLRT2-UNC5 signaling

  Cameron L. Prigge, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-FLRT2 1:250 (AF2877 R&D Systems); mouse anti-GAD65 1:1000 (MAB351 ...
• NOTCH3 active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model

  Daniel V. Oliveira, et al., bioRxiv - Neuroscience 2022

  Quote: ... 100 µL of a solution containing 0.001 mg/mL of the detection biotinylated polyclonal antibody (BAF1559; R&D Systems) raised against NOTCH3 ECD and 2.5% BSA was added and was used for detection followed by agitation for 2h at RT ...
• NOTCH3 active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model

  Daniel V. Oliveira, et al., bioRxiv - Neuroscience 2022

  Quote: ... Whole blood serum samples were diluted 1:40 in 100 µL of reagent diluent (DY995; R&D Systems) and recombinant human NOTCH3 ECD (1559-NT-050 ...
• NOTCH3 active immunotherapy reduces NOTCH3 deposition in brain capillaries in a CADASIL mouse model

  Daniel V. Oliveira, et al., bioRxiv - Neuroscience 2022

  Quote: ... The plates were washed 3 times with washing buffer and a horseradish peroxidase-streptavidin (HRP-Strep) complex (R&D Systems DY998) diluted 1:25 in reagent diluent was added to the plates and incubated for 40 minutes with agitation ...
• Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver

  Keshav Thapa, et al., bioRxiv - Physiology 2022

  Quote: ... Cells were thereafter lysed with Lysis Buffer #6 (R&D Systems) and assayed for the expression levels of phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187 ...
• Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver

  Keshav Thapa, et al., bioRxiv - Physiology 2022

  Quote: ... and phospho-JNK (T183/Y185 for JNK1/2 and T221/Y223 for JNK3) with commercial kits (DuoSet IC ELISA, R&D Systems, # DYC1018B and # DYC1387B) according to manufacturer’s instructions ...
• Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver

  Keshav Thapa, et al., bioRxiv - Physiology 2022

  Quote: ... phospho-Akt (R&D Systems, #AF887) and Akt (R&D Systems ...
• Endothelial FABP4 constitutes the majority of basal circulating hormone levels and regulates lipolysis-driven insulin secretion

  Karen E. Inouye, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). For Figure 8C WT mouse plasma FABP4 was measured using a commercially available assay for mouse FABP4 (Mouse Adipocyte FABP (FABP4 ...
• Control of lipolysis by a population of oxytocinergic sympathetic neurons

  Erwei Li, et al., bioRxiv - Physiology 2022

  Quote: ... cAMP levels were measured from cell lysates using the cAMP Parameter Assay Kit (R&D systems, KGE002B).
• Semaphorin 4B is an ADAM17-cleaved inhibitor of adipocyte thermogenesis

  Abdulbasit Amin, et al., bioRxiv - Physiology 2022

  Quote: ... HB-EGF and Amphiregulin using commercial ELISA kits (88-7324-22 eBioscience, DY8239-05, R&D Systems, DY989, R&D Systems, respectively).
• Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

  N Kurgan, et al., bioRxiv - Physiology 2022

  Quote: ... Sclerostin (cat#MAB1406) and Lrp4 (cat#MAB5948) antibodies was purchased from R&D Systems, Inc ...
• Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

  N Kurgan, et al., bioRxiv - Physiology 2022

  Quote: ... and were further stratified to either treatment of 5 d/wk injections of recombinant sclerostin (0.1 mg/kg body mass) (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline control ...
• Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

  N Kurgan, et al., bioRxiv - Physiology 2022

  Quote: Mice either had subcutaneous (s.c) recombinant sclerostin (cat# 1589-ST; R&D Systems, Minneapolis, Minnesota, USA) or saline injections 5 d/week for 4 weeks ...
• Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

  N Kurgan, et al., bioRxiv - Physiology 2022

  Quote: ... purchased from R&D Systems, Minneapolis ...
• Sclerostin influences exercise-induced adaptations in body composition and white adipose tissue morphology in male mice

  N Kurgan, et al., bioRxiv - Physiology 2022

  Quote: ... Serum sclerostin was analysed in duplicate using a Quantikine enzyme-linked immunoassay kit (cat#MSST00; R&D Systems, Minneapolis, Minnesota, USA) according to manufacturer’s instructions.
• Melanocortin 1 receptor regulates cholesterol and bile acid metabolism in the liver

  Keshav Thapa, et al., bioRxiv - Physiology 2022

  Quote: ... and Akt (R&D Systems, #MAB2055) over-night at + 4°C ...
• Soluble VE-cadherin disrupts endothelial barrier function via VE-PTP/RhoA signalling

  Juna-Lisa Knop, et al., bioRxiv - Physiology 2022

  Quote: ... or immunofluorescent (IF) staining as indicated below: Antibody against VE-cadherin extracellular domain (WB 1:1000, IF 1:50, R&D Systems, MAB9381), antibody against VE-cadherin intracellular domain (WB 1:1000 ...
• ATGL is differentially required for adipocyte FABP4 secretion in vivo and ex vivo

  Kacey J. Prentice, et al., bioRxiv - Physiology 2022

  Quote: ... HRP-tagged clone 351.4.5E1.H3 for detection) and recombinant human FABP4 as a standard (R&D Systems, cat# DY3150-05). Plasma insulin was assessed using a Crystal Chem Ultra Sensitive Mouse Insulin ELISA ...
• Soluble VE-cadherin disrupts endothelial barrier function via VE-PTP/RhoA signalling

  Juna-Lisa Knop, et al., bioRxiv - Physiology 2022

  Quote: ELISA-based quantification of soluble VE-cadherin in cell culture supernatants (R&D Systems, Wiesbaden-Nordenstadt ...
• FGF21 has a sex-specific role in calorie-restriction-induced beiging of white adipose tissue in mice

  Mariah F. Calubag, et al., bioRxiv - Physiology 2022

  Quote: ... Blood FGF21 levels were assayed by a mouse/rat FGF-21 quantikine ELISA kit (MF2100) from R&D Systems (Minneapolis, MN, USA).
• Prorenin from renal tubules is a major driver of diabetic kidney disease

  Anna Federlein, et al., bioRxiv - Physiology 2022

  Quote: ... polyclonal goat anti-CD31 antibody (dilution 1:200) (AF3628, R&D Systems, USA), monoclonal rat anti-F4/80 antibody (dilution 1:200 ...
• Clemastine fumarate enhances myelination and promotes functional recovery in a syndromic ASD mouse model of Pitt-Hopkins Syndrome

  Joseph F. Bohlen, et al., bioRxiv - Neuroscience 2022

  Quote: ... and 30ng/mL rhPDGF-AA (R&D systems, 221-AA), with a half media exchange on DIV4 ...
• Nuclear receptor ligand screening in an iPSC-derived in vitro blood-brain barrier model identifies new contributors to leptin transport

  Yajuan Shi, et al., bioRxiv - Neuroscience 2022

  Quote: Recombinant human leptin (R&D Systems #398-LP) was reconstituted at 1 mg/mL in sterile 20 mM Tris-HCl (Fisher Scientific #MT46031CM ...
• Integrating inflammatory biomarker analysis and artificial intelligence-enabled image-based profiling to identify drug targets for intestinal fibrosis

  Shan Yu, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... each well was then filled with 25 μL/well recombinant human TNFα (R&D systems, cat #: 210-TA-100) diluted in cell culture medium (final concentration 10 ng/mL ...
• Integrating inflammatory biomarker analysis and artificial intelligence-enabled image-based profiling to identify drug targets for intestinal fibrosis

  Shan Yu, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... A customed kit for pro-fibrotic biomarker detection was ordered from R&D systems. The experiment was performed according to the manufacturer’s instruction ...
• Effects of anti-fibrotic standard of care drugs on senescent human lung fibroblasts

  Stephanie B. Garcia, et al., bioRxiv - Pharmacology and Toxicology 2022

  Quote: ... and WNT16 levels were determined in senescent fibroblast lysates using a standardized sandwich Enzyme-Linked Immunoassay (ELISA) technique (R&D Systems, Minneapolis, MN, USA), according to manufacturer’s protocol.
• Functional Recovery from Human Induced Pluripotent Stem Cell-Derived Dopamine Neuron Grafts is Dependent on Neurite Outgrowth

  Rachel Hills, et al., bioRxiv - Neuroscience 2022

  Quote: ... TGFB3 (1ng/mL, R&D Systems) and DAPT (10μM ...
• Asynchronous excitatory neuron development in an isogenic cortical spheroid model of Down syndrome

  Zhen Li, et al., bioRxiv - Neuroscience 2022

  Quote: ... 10 ng/ml platelet-derived growth factor-AA (PDGF-AA, R&D systems) and insulin-like growth factor-1 (IGF-1 ...
• Asynchronous excitatory neuron development in an isogenic cortical spheroid model of Down syndrome

  Zhen Li, et al., bioRxiv - Neuroscience 2022

  Quote: ... goat anti-SOX2 (1:250 R&D Systems, cat. number: AF2018), rabbit anti-TBR1 (1:250 ...
• Asynchronous excitatory neuron development in an isogenic cortical spheroid model of Down syndrome

  Zhen Li, et al., bioRxiv - Neuroscience 2022

  Quote: ... and Neurotrophin-3 (NT-3, 20ng/ml, R&D systems) were used for neural differentiation between days 27 and 41 ...
• Asynchronous excitatory neuron development in an isogenic cortical spheroid model of Down syndrome

  Zhen Li, et al., bioRxiv - Neuroscience 2022

  Quote: ... Brain Derived Neurotrophic Factor (BDNF, 20ng/ml, R&D systems) and Neurotrophin-3 (NT-3 ...
• Asymmetric activation of microglia in the hippocampus drives anxiodepressive consequences of trigeminal neuralgia

  Li-Qiang Chen, et al., bioRxiv - Neuroscience 2022

  Quote: ... ELISA was conducted according to manufacturer’s instructions (R&D Systems, #RLB00, Minneapolis, MN) to assay IL-1β content in the supernatant.