6 1 4 dioxa 8 azaspiro 4.5 decan 8 yl 3 hydroxy 2 iminopyrimidin 4 amine citations for Santa Cruz

Quote: ... The primary antibodies (anti-Cd31, goat polyclonal, R&D Systems #AF3628, 1:100; anti-VEGFA, rabbit monoclonal, abcam #ab52917, 1:200; anti-Endomucin, rat, Santa Cruz #sc-65495, 1:100 in AK Diluent) and secondary antibodies (anti-goat 546 ...

A novel non-catalytic scaffolding activity of Hexokinase 2 contributes to EMT and metastasis

Catherine Blaha, et al., bioRxiv - Cancer Biology 2021

Quote: ... Protein extracts were incubated with each antibody with rotation for 2 hr and then added to protein A/G agarose beads (Santa Cruz, sc-2003) for an additional 1 hr ...

Chromatin binding of survivin regulates glucose metabolism in the IFN-γ producing CD4⁺ T cells

Malin C. Erlandsson, et al., bioRxiv - Immunology 2021

Quote: ... JUND (D-9, sc-271938), SMAD3 (38-Q, sc-101154), MAX (H-2, sc-8011), and MYC (9E10, sc-40) (all, Santa Cruz Biotechnology, USA) followed by peroxidase-conjugated anti-mouse antibodies (NA931 ...

Quantification of extracellular vesicles with unaltered surface membranes using an internalized oligonucleotide tracer and droplet digital PCR applied to modeling of in vivo kinetics

Thomas De Luca, et al., bioRxiv - Molecular Biology 2020

Quote: Mouse monoclonal primary antibodies included: anti-CD63 (clone MX-49.129.5, 2 μg/mL, cat. no. sc-5275, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CD81 (clone 1.3.3.22 ...

In vivo discovery of RNA proximal proteins in human cells via proximity-dependent biotinylation

Xianzhi Lin, et al., bioRxiv - Molecular Biology 2020

Quote: ... were washed with 200 μL HBS (150 mM NaCl, 10 mM HEPES, pH7.5 by KOH) and incubated with 2 μg anti-HA (Santa Cruz, Cat.# sc-7392) or 2 μg rabbit IgG isotype (Thermo Fisher Scientific ...

L-WNK1 is required for BK channel activation in intercalated cells

Evan C. Ray, et al., bioRxiv - Physiology 2021

Quote: ... Kidney cortex cryosections were labeled with a polyclonal goat anti-aquaporin-2 (Aqp2) antibody (0.2 μg/ml, sc-9882; Santa Cruz Biotechnology, Santa Cruz CA, USA), and detected using a donkey anti-goat immunoglobulin G conjugated to Cyanine Cy3 (0.5 μg/ml ...

CPEB2 m⁶A Methylation Regulates BTB Permeability via Affecting Splicing Factor SRSF5 Stability

Mengyang Zhang, et al., bioRxiv - Cancer Biology 2020

Quote: ... containing 5% skimmed milk powder for 2 hours at room temperature.Incubate primary antibody overnight at 4°C.The membrane was washed three times with TBST and then incubated at room temperature with conjugated HRP secondary antibodies for 2 hours.ECL (enhanced chemiluminescence kit, Santa Cruz Biotechnology, Dallas, TX) detection system (Thermo Scientific ...

NF-Y controls fidelity of transcription initiation at gene promoters through maintenance of the nucleosome-depleted region

Andrew J Oldfield, et al., bioRxiv - Genomics 2019

Quote: ... RNA Pol II and NF-YA levels were measured using an anti-Rpb3 antibody60 and anti-NF-YA antibody (G-2; Santa Cruz, sc-17753X) respectively ...

A SARS-CoV-2 neutralizing antibody protects from lung pathology in a COVID-19 hamster model

Jakob Kreye, et al., bioRxiv - Immunology 2020

Quote: Recombinant SARS-CoV-2-S1 protein produced in HEK cells (Creative Diagnostics, DAGC091) was covalently labeled using CruzFluor647 (Santa Cruz Biotechnology, sc-362620) according to the manufacturer’s instructions.

Loss of function in the autism and schizophrenia-associated gene CYFIP1 in human microglia supports a role in synaptic pruning

Steven D. Sheridan, et al., bioRxiv - Neuroscience 2022

Quote: ... iPSCs were passaged as needed in the presence of 2 μM Rho-associated kinase (ROCK) inhibitor (RI) thiazovivin (Santa Cruz Biotechnology, #SC-361380). Prior to transfection ...

Ccr4-Not ubiquitin ligase signaling regulates ribosomal protein homeostasis and inhibits 40S ribosomal autophagy

Daniel L. Johnson, et al., bioRxiv - Molecular Biology 2023

Quote: ... catalog number sc7392), α-GFP (clone B-2, catalog number sc9996), and α-ubiquitin (clone P4D1, catalog number sc8017) antibodies were purchased from Santa Cruz Biotechnology ...

A PARP14/TARG1-Regulated RACK1 MARylation Cycle Drives Stress Granule Dynamics in Ovarian Cancer Cells

Sridevi Challa, et al., bioRxiv - Cell Biology 2023

Quote: ... Volumes of lysate containing equal amounts of total protein were used to immunoprecipitate PARP14 by incubating with 2 µg of a mouse monoclonal antibody against PARP14 (Santa Cruz, sc-377150), and protein G agarose beads overnight at 4°C with gentle mixing on a nutator at 4°C ...

mRNA decapping machinery targets LBD3/ASL9 transcripts to authorize developmental reprogramming in Arabidopsis

Zhangli Zuo, et al., bioRxiv - Plant Biology 2020

Quote: ... and incubated 1hr to overnight with primary antibodies (anti-GFP (AMS Biotechnology 1:5.000, anti-HA 1:1,000 (Santa Cruz)) ...

Rhes, a Striatal Enriched Protein, Regulates Post-Translational Small-Ubiquitin-like-Modifier (SUMO) Modification of Nuclear Proteins and Alters Gene Expression

Oscar Rivera, et al., bioRxiv - Biochemistry 2020

Quote: ... Antibodies for GST-HRP (1:5000, #sc-138), and Myc (1:3000, #sc-40) were obtained from Santa Cruz Biotechnology ...

Defective endosome-TGN retrograde transport promotes NLRP3 inflammasome activation

Zhirong Zhang, et al., bioRxiv - Immunology 2021

Quote: ... BMDMs and THP-1 cells were infected in fresh medium containing 1 µg/ml polybrene (sc-134220, Santa Cruz) and 25% lentivirus-contained supernatant.

Mechanisms of A-type lamin targeting to nuclear ruptures are disrupted in LMNA- and BANF1-associated progerias

Rhiannon M. Sears, Kyle J. Roux, bioRxiv - Cell Biology 2022

Quote: ... Cells were labeled for 1 h in primary antibodies: goat anti-preLaA (1:50; sc-6214, Santa Cruz Biotechnology) mouse anti-LaA/C (1:100 ...

A low dose of simvastatin enhanced the therapeutic efficacy of mesenchymal stem cell (MSC) transplantation in skin wound healing in diabetic mice associated with increases in pAkt, SDF-1, and angiogenesis

Supakanda Sukpat, et al., bioRxiv - Physiology 2019

Quote: ... The sections were incubated with rabbit polyclonal SDF-1 antibody (sc-28876, Santa Cruz, USA, at 1:25 dilution). After washing in wash buffer ...

The Shu Complex Prevents Mutagenesis and Cytotoxicity of Single-Strand Specific Alkylation Lesions

Braulio Bonilla, et al., bioRxiv - Genetics 2021

Quote: ... 1:1000 with secondary antibody anti-rabbit (Jackson ImmunoResearch Laboratories 1:10,000] or αKar2 antibody [Santa Cruz (sc-33630); 1:5000 with secondary antibody anti-rabbit (Jackson ImmunoResearch Laboratories 1:10,000] as a loading control.

Nup358 regulates remodelling of ER-mitochondrial contact sites and autophagy

Misha Kalarikkal, et al., bioRxiv - Cell Biology 2021

Quote: ... Anti-myc (sc-401; WB, 1000), -Tom20 (sc17764; WB, 1:3,000) and GAPDH (sc-166574; WB, 1:5,000) were from Santa Cruz Biotechnology Inc ...

Salmonella Enteritidis Effector AvrA suppresses autophagy by reducing Beclin-1 protein

Yang Jiao, et al., bioRxiv - Microbiology 2020

Quote: ... anti-c-myc (PE10, 1:1,000, SC-40), anti-HA-probe (F-7, 1:1,000, SC-7392) (Santa Cruz Biotechnology ...

PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteins

Ying Liu, et al., bioRxiv - Microbiology 2020

Quote: ... Cells were fixed 20h post infection and stained with PSGL-1 antibody (Santa Cruz Biotech, sc-13535, 1:200), gp41 antibody (NIH AIDS Reagent Program ...

In vivo base editing rescues photoreceptors in a mouse model of retinitis pigmentosa

Jing Su, et al., bioRxiv - Genetics 2022

Quote: ... The primary antibodies used for RPE/choroid/sclera was rat anti-ZO-1 (1:100; Santa Cruz; sc-33725); those used for retina were rabbit anti-S opsin (1:50 ...

Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

Eliana Nachman, et al., bioRxiv - Molecular Biology 2019

Quote: ... and immunoblotted with the Tau antibody A-10 (1:1 000 - 10 000, mouse, sc-390476, Santa Cruz Biotechnology) or anti-GFP (1:10 000 ...

Inhibition of c-Jun in AgRP neurons mediates chronic stress-induced anxiety-like behaviors and colitis susceptibility

Fuxin Jiao, et al., bioRxiv - Neuroscience 2022

Quote: ... and c-Fos (1:1000, Cell Signaling Technology, mAb2250 or 1:500, Santa Cruz Biotechnology, Santa Cruz, CA, USA). c-Fos staining was coupled with a TSA Plus Fluorescein KIT (NEL741001KT ...

Optogenetic control of PRC1 reveals that bridging fibers promote chromosome alignment by overlap length-dependent forces

Mihaela Jagrić, et al., bioRxiv - Cell Biology 2020

Quote: ... Following primary antibodies were used: mouse anti-PRC1 monoclonal antibody (1:100; C-1; sc-376983, Santa Cruz Biotechnology), rabbit anti-α-tubulin polyclonal antibody (1:100 ...

Influence of the TorD signal peptide chaperone on Tat-dependent protein translocation

Umesh K. Bageshwar, et al., bioRxiv - Biochemistry 2021

Quote: ... blocked membranes were incubated (1 h) first with mouse anti-6xHis polyclonal antibodies (1:5000; Santa Cruz Biotechnology, Inc.) and then with rabbit anti-mouse antibodies conjugated with HRP (1:10,000 ...

Labshake search