Labshake search
Citations for Santa Cruz :
2401 - 2450 of 2563 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2019Quote: ... MA, USA), phospho Histone H2A.X (D17A3) (Cell Signalling Technology, Danvers, MA, USA) and CD86 B7-2 (GL-1) (Santa Cruz Technologies) combined with Alexa 647 and Alexa 488 conjugated secondary antibodies (Abcam ...
-
bioRxiv - Cell Biology 2019Quote: ... Respective cells were incubated with anti-c-Myc antibody (9E10, mouse monoclonal, 2 mg/ml; Santa Cruz Biotechnology, Dallas, TX, USA) for 1 h at room temperature ...
-
bioRxiv - Developmental Biology 2019Quote: ... The cells were then incubated with anti-UCHL1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-25800, diluted 1:200), GFRα1 (Santa Cruz Biotechnology ...
-
bioRxiv - Cancer Biology 2019Quote: ... 1×105 cells were pre-incubated for 1-2 h in N2 medium without antibiotics and 1 μg/μl of polybrene (Santa Cruz) to increase transduction efficiency ...
-
bioRxiv - Immunology 2019Quote: ... Endogenous ELF1 protein levels from cell lysates or mouse lung homogenates were measured by western blotting using anti-ELF1 antibody (1:5000, Santa Cruz).
-
bioRxiv - Pathology 2020Quote: ... Transfected cells were selected by selection media containing 2 μg/ml puromycin dihydrochloride (cat. no. sc-108071; Santa Cruz Biotechnology, Inc.). After confirming the decrease in LOXL2 expression by reverse transcription-quantitative polymerase chain reaction (RT-qPCR ...
-
bioRxiv - Pathology 2020Quote: ... HK-2 cells were treated with media containing 5 μg/ml of polybrene (cat. no. sc-134220; Santa Cruz Biotechnology, Inc.), and then LOXL2 shRNA lentiviral particles and control shRNA lentiviral particles of 1 and 2 multiplicity of infection (MOI ...
-
bioRxiv - Immunology 2019Quote: Cells were analysed for ligand anchor or HLA-A*02 surface expression by flow cytometry using anti-HA-Tag antibody Alexa Fluor 647 (6E2; Cell Signalling Technology) and anti-HLA-A2 FITC antibody (BB7.2; Santa Cruz Biotechnology) respectively (BD FACSCalibur™ ...
-
bioRxiv - Cell Biology 2020Quote: ... After a further centrifuge red blood cells were lysed in 1 ml of RBC lysis buffer (Santa Cruz Biotechnology sc-296258) in ice for 2.5 minutes ...
-
bioRxiv - Immunology 2020Quote: ... and cells were re-suspended in 100 μl 1:500 rabbit anti-ASC (N-15)-R (Santa Cruz, sc-22514-R) PBS/3%FCS/0.1% saponin and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2020Quote: Human T cells (2-5 x 106) were transfected with 3 μg of TERT KO CRISPR/Cas9 plasmid (h) (sc-400316, Santa Cruz) according to the manufacturer instructions ...
-
bioRxiv - Genetics 2019Quote: ... the proteins from cotransfected cell lysates after 24 hours were precipitated with an antibody against the N-terminus of ZP1 (G-20, Santa Cruz). Then ...
-
bioRxiv - Immunology 2022Quote: ... cells were spun at 1500rpm in 0.3% Saponin for 10min to permeabilize cells and then incubated with FITC-conjugated ErbB4 (Santa Cruz Biotech) in Saponin to maintain permeabilization ...
-
bioRxiv - Neuroscience 2022Quote: The SPPL2b gene was knocked out in HEK293 WT cells by using the SPPL2b CRISPR/Cas9 Knockout Plasmid kit from Santa Cruz Biotechnology® (sc-405646) ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunostaining was then performed by treating cells with a 1:100 dilution of anti-YAP antibody (Santa Cruz Biotechnology; sc-101199) in blocking buffer for 1 h ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were incubated in RPMI (with 5% goat serum) with 1:200 dilution of specific anti-TLR4 (Santa Cruz sc-293072) antibodies at 4°C for 1 hr then washed twice in cold RPMI to remove unbound antibodies ...
-
bioRxiv - Molecular Biology 2022Quote: ... and then 100 μl of cell lysate was incubated with an anti-COPA antibody (sc-398099, Santa Cruz Biotechnology, Dallas, TX). Mouse IgG ...
-
bioRxiv - Cell Biology 2022Quote: ... Mac-1-HEK293 cells in which CD47 gene expression was disrupted were generated using CD47 CRISPR/Cas9 KO Plasmid (h) obtained from Santa Cruz Biotechnology (catalog #sc-400508) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells were first incubated overnight at 4°C and 300rpm with p21WAF1/CIP1 (Santa Cruz, SC-817, 1:200, 1.0µg/mL), suspended in a 2.5% NGS solution ...
-
bioRxiv - Genetics 2023Quote: Cells were washed twice with PBS and fixed with Paraformaldehyde solution 4% in PBS (Santa Cruz Biotechnology, CAS 30525-89-4) for 10 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2024Quote: ... all AGPS KO single cell clones were generated via transient transfection with mouse AGPS CRISPR/Cas9 KO Plasmids (Catalog no. sc-432759, Santa Cruz) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... the following day these cells were transduced with the addition of 100 μL concentrated virus and polybrene (4 μg/mL; Santa Cruz) to the cell medium ...
-
bioRxiv - Biophysics 2024Quote: ... The cell pellets were washed with PBS (Quality Biological) and then resuspended in 100ul of RIPA lysis buffer (Santa Cruz Biotechnology) containing protease inhibitors ...
-
bioRxiv - Pathology 2023Quote: ... Immune cell recruitment and cytokine expression were assessed using antibodies against CD45 (1:100, SC-70690, Santa Cruz, Dallas, TX, USA). Residual human IgG in the DDS and in the ocular tissues was determined using goat anti-human IgG antibody (A-21089 ...
-
bioRxiv - Cell Biology 2023Quote: Stable RPE1 cells grown onto glass coverslips during the indicated times were fixed with 4% PFA solution in PBS (Santa Cruz) and permeabilized with 0.20% Triton X-100 in PBS for 15 min ...
-
bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Neuroscience 2023Quote: GL261 cells were transfected with a green fluorescent protein (CopGFP) lentiviral particle (sc-108,084; Santa Cruz Biotechnology (Santa Cruz, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2023Quote: ... GFP-TIFA-expressing cells were incubated overnight at 4°C in 0.2% saponin with a mouse monoclonal anti-myc antibody (9E10, Santa Cruz Biotechnology) or with a mouse monoclonal NF-κB p65 antibody (Sc-8008) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The HUVECs were incubated with conditioned media of control and knockdown cells with or without IgG and PEDF antibodies (Santa Cruz). The migration ability of the HUVECs was evaluated by measuring the gap widths at time intervals of 0 and 24 h ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were seeded in microslide channels at a final concentration of ∼5×105 cells/ml in 120 µL cell culture media containing 40 μM BrdU:BrdC mix (3:1, Cat.# B5002, Sigma and Cat.# 284555, Santa Cruz Biotec) and grown for 17-24 h ...
-
Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... PS1 was knocked out of HEK 293 cells using Presenilin 1 CRISPR/Cas9 KO plasmid in conjunction with Presenilin 1 HDR Plasmid from Santa Cruz Biotechnology (sc-401227 and sc-401227-HDR ...
-
bioRxiv - Cancer Biology 2023Quote: ... β-actin (12620S) were obtained from Cell Signalling Technologies (Beverly, MA, USA) and the antibody against PCNA (sc25280) were purchased from Santa Cruz Biotechnology (Santa Cruz ...
-
bioRxiv - Cancer Biology 2023Quote: ... Target cells were plated 24 hours before infection and then exposed to viral supernatant supplemented with 20 μg/ml polybrene (Santa Cruz). Mouse cell lines were then selected for at least 72 hours with 4 μg/ml puromycin (Corning) ...
-
bioRxiv - Cancer Biology 2023Quote: HCT116 p53 null or knockout cells were used as parent cell line to generate a short telomere version using commercial hTERC knockdown plasmid and a scrambled control from Santa Cruz Biotechnology ...
-
bioRxiv - Systems Biology 2024Quote: ... Reporter cells were then transduced with the virus in the presence of 8 µg/ml polybrene (Santa Cruz Biotechnology, SACSC-134220). After 7 days of transduction ...
-
bioRxiv - Cancer Biology 2024Quote: ... efficiently transduced cells were selected by two rounds of 5-day treatment with 2.5 µg/ml puromycin (sc-108071A, Santa Cruz Biotechnology). Individual cell clones were isolated by limiting dilution seeding in 96-well plates and exposed to 2.5 µg/ml puromycin every 6th passage.
-
bioRxiv - Molecular Biology 2024Quote: Antibody against HAND1 (sc-390376) (WB: 1:1000, IF for embryos: 1:100, IF for cells: 1:200) was purchased from Santa Cruz. Antibody against FOXF1 (Abclonal ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1000 µg of total protein suspended in 500µL of cell lysate was precleared with 20 µL of protein A/G plus agarose beads (Santa Cruz Biotechnology) and 1 µL of normal rabbit IgG (Cell Signalling ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were lysed in ice-cold RIPA lysis buffer containing protease inhibitors (sc-24948, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and centrifuged at 12,000 rpm for 20 min at 4 °C ...
-
bioRxiv - Cancer Biology 2021Quote: ... the cells were lysed in ice-cold RIPA lysis buffer containing protease inhibitors (sc-24948, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and centrifuged at 12,000 rpm for 20 min at 4 °C ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were incubated with p53 antibody Alexa Fluor® 546 (Santa Cruz, sc-99 AF546, 1:50 dilution, 4 μg/ml) in dark for one hour in room temperature ...
-
bioRxiv - Biophysics 2020Quote: ... the cells were incubated with p53 antibody Alexa Fluor® 647 (Santa Cruz, sc-126 AF647, 1:50 dilution, 4 μg/ml) in dark overnight in 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... the cells were then incubated in anti TFAM-Alexa 546 antibody (TA332462 self-conjugated or sc-166965; Santa Cruz Biotechnology, Dallas, USA).
-
bioRxiv - Cell Biology 2020Quote: The THP-1 monocytic cell line was transduced with gene-silencing Short-hairpin RNA (ShRNA) lentiviral particles (Santa Cruz Biotech, TX, USA). Briefly ...
-
bioRxiv - Microbiology 2020Quote: ... the cells were grown in 6-well tissue culture plates and transfected with 80 pmol EGFR siRNA (sc-29301, Santa Cruz Biotechnology), and EphA2 siRNA (sc-29304 ...
-
bioRxiv - Systems Biology 2020Quote: ... the DNA bound to Fur from formaldehyde cross-linked cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell extracts were prepared 48 hours later and immunoprecipitations were performed (27) using rabbit anti-HA antibody (sc-805, Santa Cruz Biotechnology). Immunoprecipitates were analyzed by immunoblot using mouse anti-Flag M2 antibody (F1804 ...
-
bioRxiv - Neuroscience 2020Quote: ... We also analysed the phenotype of Zif268 cells by incubating one-in-ten sections with a rabbit anti-Zif268 antibody (1:500, Santa Cruz Biotechnology) and a mouse monoclonal anti-NeuN antibody (1:500 ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The cell monolayer was washed with cold HBSS three times and lysed by adding 50 μL/well of 1X radioimmuno-precipitation assay (RIPA) cell lysis buffer (Santa Cruz Biotechnology). The plates were maintained at constant agitation at 4°C for 20 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... To identify PtrR binding sites for each strain the DNA bound to PtrR from formaldehyde cross-linked cells was isolated by chromatin immunoprecipitation (ChIP) with the antibodies that specifically recognize the myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen ...