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Citations for Santa Cruz :
2101 - 2150 of 2415 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Cells transfected with the PX459 vector were selected with 1 ug/mL puromycin (Santa Cruz Biotechnology, sc-108071B) 48 h after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were rinsed with 10 ml ice-cold harvest buffer (MgCl2 10 mM, cycloheximide (Santa Cruz sc-3508A) 100 µg/ml in PBS) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleared cell lysates were incubated for 2 hours with Protein A/G agarose beads (Santa Cruz, SC-2003) that were hybridized with EGFR antibody (Thermo ...
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bioRxiv - Pathology 2022Quote: ... Cell extracts (800 μg) were incubated with one microliter of anti-YAP antibody (Santa Cruz Biotechnology, SCBT-101199) or anti-TAZ antibody (Cell Signaling ...
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bioRxiv - Immunology 2022Quote: ... the supernatant was then used to transduce CD8+ 58-/- cells with 0.8 μg/mL polybrene (Santa Cruz Biotech). Cells were spinfected at 1000×g and 32°C for 1.5 hours ...
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bioRxiv - Immunology 2022Quote: ... cells or sections were incubated with mouse anti-8-OHdG (sc-393871, Santa Cruz Biotechnology, Dallas, TX, USA), anti-mouse KIM-1 (AF1817 ...
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bioRxiv - Microbiology 2021Quote: ... The cells were transduced with lentivirus in the presence of 8 μg/ml polybrene (Santa Cruz Biotechnology; #SC134220), centrifuged at 1000g for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... NeuroD1 or Ascl1 retrovirus was added in GBM cells together with 8 μg/ml Polybrene (Santa Cruz Biotechnology). Culture medium was completely replaced by neuronal differentiation medium (NDM ...
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bioRxiv - Pathology 2022Quote: ... Cells were inoculated with viruses at MOI>1 in the presence of 4ug/ml of Polybrene (Santa Cruz). Stable NIKS populations were generated following selection with puromycin (10ug/ml) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK293 cells were transfected by combining plasmid DNA with PEI (linear 25 kDa polyethylenimine; Santa Cruz, SC-360988A) at a ratio of 1:3 (w:w ...
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bioRxiv - Cell Biology 2019Quote: CRISPR (Ran et al., 2013) knock out cell lines were generated using the CRISPR plasmid CD44 (Santa Cruz Biotechnologies ...
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bioRxiv - Immunology 2020Quote: ... T cells were analysed 48-72h after nucleofection with siRNAs to TERT (10 nM, sc-156050, Santa Cruz) or siCtrl (10 nm ...
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bioRxiv - Immunology 2021Quote: Lentiviruses were prepared by transient transfection of HEK293T cells with linear 25 kDa polyethylenimine (PEI, Santa Cruz Biotechnology) at a 3:1 mass ratio of PEI to DNA ...
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bioRxiv - Molecular Biology 2019Quote: ... cells (confluency between 0.4 – 2 ×106) were directly stained with anti-Bu-1 conjugated with phycoerythrin (Santa Cruz 5K98-PE 70447 or Invitrogen 21-1A4-PE MA5-28754 ...
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bioRxiv - Cancer Biology 2022Quote: Cells were harvested and lysate were prepared in 1X RIPA lysis buffer (Santa Cruz Biotechnology, cat# sc-24948) by sonication followed by the measurement of protein concentration using BCA Protein Assay Kit (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were washed three times and incubated with diluted rabbit anti-paxillin (Santa Cruz Biotechnology, cat# sc-5574) primary antibody overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... cell lysates were incubated with 30 μl slurry of goat polyclonal HA (Santa Cruz, Cat. sc-805 AC) or Myc (Santa Cruz ...
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bioRxiv - Neuroscience 2022Quote: ... The cell lysate was precleared by incubating Protein A/G Plus-agarose beads (sc-2003, Santa Cruz Biotechnology) for 1 h at 4℃ ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 Million cells were used and incubated with 2 µg YAP antibodies (Abcam ab52771, Santa Cruz sc-101199). All following steps were performed as described43,44 ...
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bioRxiv - Cell Biology 2023Quote: ... Pex16/MARCH5 dko and Pex26/MARCH5 dko HeLa cells were generated using double nickase plasmids from Santa Cruz Biotechnology ...
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bioRxiv - Genetics 2023Quote: ... The cell lysates were incubated with protein A/G beads (sc-2003) (Santa Cruz Biotechnology, Dallas, TX, USA) for 30 min to eliminate the background signal ...
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bioRxiv - Molecular Biology 2023Quote: ... One hour post irradiation the cells were fixed then stained with anti-YAP antibody (Santa Cruz, Texas, USA). 2ry mouse-C5 antibody (Jacksons Lab ...
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bioRxiv - Cell Biology 2023Quote: ... cells were further incubated with the Alexa Fluor 488-conjugated anti-lamin B1 antibody (Santa Cruz, #sc-365214AF488) and TO-PRO-3 dye to visualize the nuclear lamina (NL ...
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bioRxiv - Cell Biology 2023Quote: ... cell extracts were incubated with 4 μg of mouse anti-DCAF1 (Santa Cruz Biotechnology, catalog no.: sc-376850) antibody overnight on a rotating wheel ...
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bioRxiv - Cell Biology 2023Quote: Cells were transfected as described above with the double nickase CRISPR Cas9 plasmid (Santa Cruz, sc-424440-NIC) or a CRISPR Control plasmid (Santa Cruz ...
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bioRxiv - Systems Biology 2024Quote: ... cells were fixed with 4% paraformaldehyde (PFA) and immunostained for Oct4 (Pou5f1) (Santa Cruz sc- 5279, 1:1,000) using an Alexa Fluor 488 secondary antibody (Invitrogen A11029 ...
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bioRxiv - Cell Biology 2023Quote: AMPKα1 and α2 knockdown experiments in C2C12 cells were performed using scrambled control (Santa Cruz Biotechnology, sc-37007) or AMPKα1/α2-specific siRNAs (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: B7-H3 (cd276) was knocked-out in tumor cell lines using CRISPR/Cas9 KO plasmids from Santa Cruz Biotechnology (human ...
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bioRxiv - Cancer Biology 2023Quote: ... coated with one of four ERα antibodies (Milipore 06-935, Abcam Ab3575, Cell Signaling #8644, Santa Cruz HC20). Excess antibody was washed before beginning pulldown ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transfected cells were selected one day after transfection with puromycin 2 μg/mL (Santa Cruz Biotech, sc-108071B) or blasticidin 10 μg/mL (Santa Cruz Biotechnology ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... embryos were incubated with a mouse monoclonal antibody against Oct4 stem cell marker (sc-5279, Santa Cruz Biotechnology), followed by an incubation with a Alexa Fluor™ 488 donkey anti-mouse IgG secondary antibody (A-21202 ...
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bioRxiv - Cell Biology 2024Quote: ... Crypts or sorted cells were lysed using RIPA buffer supplemented with protease and phosphatase inhibitors (Santa Cruz Biotechnology). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... The resulting cell lysates were each incubated overnight at 4 [with Dynabeads (Sc-2003, Santa Cruz Biotechnology, USA) pre-conjugated with antibodies against the viral HA protein (GTX127357 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with Mouse anti-MDR1/ABCB1 antibody conjugated to Alexa Fluor 488 (Santa Cruz Biotechnology - sc55510 AF488) overnight in the dark at 4 °C before incubation with Mouse IgG Fc binding protein conjugated to CruzFluor 488 (Santa Cruz Biotechnology-sc533653 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells stably expressing the Atg5 gene were optimised for protein expression via 2 μg/ml puromycin (Santa Cruz Biotechnology) selection for 7 days.
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bioRxiv - Cell Biology 2020Quote: ... Cells were then washed three times with PBS and fixed with 4% para-formaldehyde (Santa Cruz Biotechnologies, Texas, US) for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Prelid1 and StARD7 genes were knocked out in C2C12 cells by transfecting CRISPR/Cas9 mouse plasmids from Santa Cruz using Viafect Transfection Reagent (Promega E4982) ...
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bioRxiv - Immunology 2021Quote: ... BMDMs and THP-1 cells were infected in fresh medium containing 1 µg/ml polybrene (sc-134220, Santa Cruz) and 25% lentivirus-contained supernatant.
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bioRxiv - Molecular Biology 2020Quote: Cells on coverslips were washed with PBS and crosslinked with 4% paraformaldehyde in PBS (Santa Cruz Biotechnology, sc-281692) for 10 minutes at room temperature ...
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Inositol depletion regulates phospholipid metabolism and activates stress signaling in HEK293T cellsbioRxiv - Cell Biology 2022Quote: The HEK293T ISYNA1 knockout cell line was constructed using a double nickase system (Santa Cruz Biotechnology sc-404785-NIC) that contains a pair of plasmids each expressing a D10A mutated Cas9 nuclease and 20 nucleotide guide RNA (gRNA ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in Perm/Wash Buffer and fixed with 2 % PFA in PBS (Santa Cruz Biotechnology, Heidelberg, Germany). Cells were analyzed with a BD LSRFortessa Flow Cytometer (BD Biosciences) ...
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bioRxiv - Developmental Biology 2019Quote: ... Flame cells were stained using an anti-acetylated α-tubulin mouse antibody at 1:500 (sc-23950, Santa Cruz) as described previously17.
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bioRxiv - Immunology 2019Quote: ... Samples were subjected to 1D SDS-PAGE and immunoblotted for ubiquitin (clone P4D1; Santa Cruz or Cell Signaling Technology) or A20 (clone 59A426 ...
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bioRxiv - Developmental Biology 2019Quote: ... Sonicated chromatin was incubated with 5 g/107 cells of the αSOX2 Y-17 (Santa Cruz, Cat#sc-17320) or 5μl of antibody/4×106 cells of the αOCT4A C30A3C1 (Cell Signaling Technology ...
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bioRxiv - Microbiology 2019Quote: ... at least 300,000 cells were lysed in 60 μl of 1X RIPA lysis buffer (sc-24948, Santa Cruz Biotechnology) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: M8/M8B-pZac2.1 plasmid transfected HEK293 cell pellets were lysed in RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948) supplemented with protease inhibitor cocktail (Roche ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5-1 mg of cell lysates were incubated with 20 µl anti-p53 (#sc-126 AC, Santa Cruz Biotechnology) or anti-FLAG (#sc-166355AC ...
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bioRxiv - Cancer Biology 2020Quote: Membranes were probed with antibodies against RIPK2 (1:1,000, Cell Signaling Technology #4142 or Santa Cruz Biotechnology #sc-166765), c-Myc (1:5,000 ...
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bioRxiv - Cell Biology 2021Quote: ... The cell pellet was washed with PBS and lysed in a RIPA Buffer System (Santa Cruz Biotechnology, Dallas, TX). Cell lysates were separated by 10% SDS-PAGE followed by electrophoretic transfer to polyvinylidene difluoride membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... The cell lysates were centrifuged and cleared with A/G beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) at 4 °C for 1 h ...