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Citations for Santa Cruz :
2201 - 2250 of 2415 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... UMUC1 (2×105) cells were transfected with 2.5 mg of HNF-3alpha CRISPR/Cas9 KO plasmid (Santa Cruz, sc-400743) using lipofectamine3000 (Thermo fisher scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then incubated with Anti-c-myc antibody conjugated with PE (sc-40, Santa Cruz Biotechnology, 1:40) and either sAB-K29 as primary antibody (3 ug/mL) ...
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bioRxiv - Cell Biology 2020Quote: ... Infections for generation of stable knockdown in HeLa and HEK293 cells were performed with shRNA lentiviral particles (Santa Cruz Biotech). Infections for generation of stable LoKI cells were performed using lentiviral particles created in-house (as described in (26)) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with virus-containing supernatants in the presence of 8 μg/mL Polybrene (Santa Cruz Biotech, sc-134220) for 24 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitation of sonicated material was performed with antibodies against VDR (D2K6W) – Cell Signaling) or IgG (Rabbit IgG sc2729 – Santa Cruz) for 16 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... the cell lysates containing 1□mg of protein with individual antibodies and Protein A/G plus-agarose (Santa Cruz Biotechnology) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stained with anti-α-tubulin (1:700, B-5-1-2, Santa Cruz, USA, cat no sc-23948), anti-CENP-C (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... cell lysates were incubated with anti-Flag (M2) beads or with protein G Plus-Agarose immunoprecipitation reagent (Santa Cruz Biotechnology) together with 1 μg of the corresponding antibodies at 4°C overnight on a roller ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were infected with lentiviral particles in the presence of 10 µg/ml of polybrene (Cat# sc-134220, Santa Cruz) and selected with puromycin at 3 µg/ml (Cat# 0240.3 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were grown on coverslips and incubated at 37°C for 6 hrs with Pladienolide B (0.5 μM, Santa Cruz) before fixation for 20 min in 4% PFA ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed in 2% formaldehyde solution by adding equal amount of 4% formaldehyde (Santa Cruz Biotechnology, cat# sc-281692) directly in the culture media and incubated for 20 minutes at room temperature ...
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bioRxiv - Physiology 2019Quote: ... cell lysates were mixed with various antibodies and precipitated with protein A/G agarose beads (Santa Cruz Biotechnology, Dallas, TX) or FLAG M2 monoclonal antibody affinity gel (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: PCa cells were co-transfected with β-Catenin-responsive TOPflash plasmid and control or siRNAs against YAP1 (Santa Cruz Biotechnology) by using X-tremeGENE Transfection Reagent (Sigma Aldrich ...
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bioRxiv - Neuroscience 2019Quote: Levels of pERK and pAKT in immortalized cells were analyzed by Western blotting using E4 pERK (1:1000, Santa Cruz Biotechnology Cat# sc-7383 ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with antibodies against procollagen I (1:100; sc-30136, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and β-tubulin (1:200 ...
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bioRxiv - Physiology 2019Quote: ... Cells were then incubated with antibodies against procollagen I (1:100; sc-30136, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and β-tubulin (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed five times with Milli-Q water and stained with Hoechst 33342 (Santa Cruz, Cat. No. SC-200908) dye at 1 μg/mL in Mili-Q water for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... C2C12 cells were first washed with cold PBS and lysed in 100 μl RIPA lysis buffer (Santa Cruz, sc-24948) in 6-well plates ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Cell lysate/antibody mixture were then incubated with protein A/G-agarose beads (Santa Cruz Biotechnology, Inc., Santa Cruz, CA) for 2-hour at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then selected for stable integrants at the FRT locus using 200 μg/ml hygromycin B (Santa Cruz biotechnology) for at least 2 weeks ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were fixed with 4% paraformaldehyde and subjected to indirect immunofluorescence using a MYC antibody (N-262, Santa Cruz Biotechnology) followed by Alexa488-conjugated secondary antibody (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: TCam-2 cells were transfected with 40 nM siRNA mixture of 3 different KIF18A sequences (Santa Cruz Biotechnology, sc-96629), PUM1 (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2020Quote: ... U2OS cell lysates were incubated with 20 µL pre-coupled antibody-protein A/G PLUS-agarose beads (Santa Cruz Biotechnology) for 3 hr at 4 °C rotating ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were edited with a dual episomal system for homology directed repair (HDR) (Santa Cruz Biotechnology, Inc., Dallas, USA). Cells were transfected with Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... Transient knockdown in mouse HL-1 cells was performed with siRNA particles targeted to mouse myoglobin (sc-35994, Santa Cruz) or non-targeting scramble siRNA (sc-37007 ...
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bioRxiv - Microbiology 2023Quote: ... A549 or HSAE cells were transduced with the lentiviruses in the presence of 10 µg/mL polybrene (Santa Cruz Biotechnology). The next day ...
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bioRxiv - Cancer Biology 2023Quote: ... media was removed and cells incubated with 20 mL of PBS containing 2 mM disuccinimidyl glutarate (DSG) (Santa Cruz Chemical) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells transfected with HA-TAp73-pcDNA3 plasmid were incubated with rabbit anti-HA antibody (Santa Cruz #805, Dallas, TX, USA) diluted 1:200 in PBS for 1h at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The filtered viruses were added to culture media of cells with polybrene (Santa Cruz, final concentration of 8 µg/mL). Fresh media were replaced 48 hours after infection ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then incubated overnight at 4°C with a mouse antibody recognizing GFP (Santa Cruz, sc-9996, 1:300) and a rabbit antibody recognizing TGN46 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were knocked down by small interfering RNA (siRNA) targeting galectin-3 (Santa Cruz Biotechnology, USA ref: sc-155994-SH) according to the manufacturer’s siRNA transfection protocol.
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bioRxiv - Microbiology 2023Quote: ... were added to the cell culture at an MOI of 0.25 in the presence of polybrene (Santa Cruz, Dallas, Texas). At 24 hours post-transduction ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were washed and incubated with secondary antibody (mouse anti-rabbit IgG-CFL 647, [Santa Cruz Biotechnology, INC.; sc-516251]) for 2 h at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Blots were incubated with secondary antibody (anti-rabbit IgG-HRP, Cell Signaling Technology; anti-mouse IgG-HRP, Santa Cruz Biotechnology) at 1:5000 for 2 hours and developed using Pierce ECL Western Blotting Substrate or SuperSignal West Femto Substrate (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2024Quote: Whole-cell protein lysates were prepared from hCO or enriched astrocytes using RIPA lysis buffer (Santa Cruz Biotechnology, sc-24948A) within HypoxyLab after 48 hr of hypoxia exposure ...
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bioRxiv - Cancer Biology 2024Quote: ... MCF10A-derived 1E7 cells cultured on tissue culture polystyrene were induced with 1 μg/mL doxycycline (204734, Santa Cruz Biotechnology) for 24 hours before being collected with 0.05% trypsin-EDTA.
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bioRxiv - Systems Biology 2023Quote: ... total PRAS40 (Cell Signaling Technologies #2691, diluted 1:1000, and 14-3-3 (Santa Cruz #sc-1657, diluted 1:5000). The following day membranes were washed 3 x 10 min in TBS-T and incubated for 1 h in species-appropriate fluorescent antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated for 48 h after adding 5 mM (final conc.) of N-methyl-DL-aspartic acid (MDLA) (Santa Cruz), solved in water ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... lysates obtained from 15-30 million cells were immunoprecipitated with 10 μg of antibodies against either Myc (Santa Cruz, #sc-764), ER-alpha (Merck ...
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bioRxiv - Molecular Biology 2021Quote: ... the same amount of cell lysate was added to the same volume of or Mouse IgG antibody (Santa Cruz, sc-2025). Following incubation ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were pulse-labelled with 10 μM EdU for 20 minutes prior to fixation in 4% paraformaldehyde in PBS (Santa Cruz) containing 0.5% Triton X-100 for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... revealed with a Cy5-goat anti-mouse (1:1,000; Jackson) and the phenotype of IdU-IR cells using Calbindin (Calb, 1:250; Santa Cruz Biotechnologies) revealed with Alexa-Fluor 647 donkey anti-goat (1/1000 ...
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bioRxiv - Biochemistry 2021Quote: ... followed by enrichment of HLA-I positive cells through up to two serial rounds of fluorescent activated cell sorting (FACS) and isolation using a pan-HLA-I antibody (W6/32; Santa Cruz) on a FACSAria II instrument (BD Biosciences) ...
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bioRxiv - Biochemistry 2021Quote: ... in press). Antibodies used to validate Sec61 and/or EMC subunit depletions in SP cells (Fig. S2) were purchased from Santa Cruz Biotechnology (goat polyclonal anti-LMNB1 (clone M-20 ...
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bioRxiv - Biochemistry 2020Quote: ... GβL (sc-425272) and SUMO1/2/3 deletion were carried out in striatal cells using CRISPR/Cas-9 tools obtained from Santa Cruz, as described before [46 ...
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bioRxiv - Immunology 2021Quote: ... 1×108 Jurkat T cells were transduced with lentivirus at an MOI of 0.5 with 8 ug/ml polybrene (Santa Cruz Biotechnology) and spinfected at 1000x g for 1.5 hours at 32°C ...
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bioRxiv - Microbiology 2021Quote: ... The phosphorylation of EGFR in mouse oral epithelial cells was determined similarly except that total EGFR was detected with anti-EGFR antibody from Santa Cruz Biotechnology (# SC373746 ...
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Unraveling the functions of uncharacterized transcription factors in Escherichia coli using ChIP-exobioRxiv - Systems Biology 2021Quote: ... coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... U2OS and HEK293 ATM KO cells were generated using the ATM CRISPR/Cas9 KO and ATM HDR plasmids (sc-400192, sc-400192-HDR from Santa Cruz) according to the manufacturer’s guidelines ...