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Citations for Miltenyi Biotec :
5001 - 5050 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and stimulated with T Cell TransAct (Diluted at 1:100, T Cell TransAct™, human, Miltenyi Biotec, #130-111-160), with fresh medium replaced every 3 days ...
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bioRxiv - Immunology 2022Quote: ... and B220 and anti-biotin MACS beads (Miltenyi Biotec). The purity of transgenic CD4+OT-II T cells was verified by flow cytometry (CD45.1+Vα2+Vβ5+CD25− ...
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bioRxiv - Genetics 2022Quote: ... or MACSxpress Neutrophil Isolation Kits human (Miltenyi Biotec) with an aim of 2 × 106 cells ...
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bioRxiv - Genetics 2022Quote: β-Thalassemia CD34+ HSPCs (Table S2) were enriched using the Miltenyi CD34 Microbead kit (Miltenyi Biotec). CD34+ HSPCs were thawed on day 0 into X-VIVO 15 (Lonza ...
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bioRxiv - Genetics 2022Quote: ... Human tonsil tissue was processed by mincing with scissors followed by transfer of up to 4g of tissue to a gentleMACS C tube (Miltenyi Biotec) containing 8 mL of phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... Anti-biotin-PE antibody was purchased from Miltenyi Biotec (130-113-291) for biotin–LPETG SrtA substrate staining ...
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bioRxiv - Immunology 2022Quote: ... using gentleMACS (Miltenyi Biotec). The suspension was passed through a 100 μm cell strainer (Falcon) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then stained with anti-human IgE FITC (IgE21; eBioscience) and anti-IgG1-PE human (IS11-3B2.2.3; Miltenyi Biotec) for 30 minutes at room temperature in the dark ...
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bioRxiv - Immunology 2022Quote: ... CD8+ SP thymocytes were isolated using the CD8a+ (Ly-2) positive selection kit per manufacturer’s instructions (Microbeads, #130-117-044; Miltenyi Biotec). Flow-through resulted in the DN population ...
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bioRxiv - Immunology 2022Quote: ... Single cells were depleted from both the CD4+ double positive (DP) and CD4+ single positive (SP) cells using a CD4+ (L3T4) positive selection kit (Microbeads; #130-117-043; Miltenyi Biotec, Gaithersburg, USA) flow-through (this contains both double-negative [DN] and CD8+SP cells) ...
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bioRxiv - Immunology 2022Quote: ... The lymph nodes were subsequently crushed through 70 μm MACS SmartStrainers (Miltenyi Biotec [Bergisch Gladbach ...
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bioRxiv - Immunology 2022Quote: ... and CD3-FITC (#130-080401; Miltenyi Biotec), fixed/lysed with Cytofix/Cytoperm (554722 ...
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bioRxiv - Immunology 2022Quote: ... single cells were passed through a 70μm MACS SmartStrainer (Miltenyi Biotec, Cat# 130-098-462) that was pre-wet with 3mL of 20% FBS in PBS into a 15mL conical tube on ice ...
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bioRxiv - Immunology 2022Quote: Untouched NK cells were isolated from frozen and thawed PBMCs using magnetic bead isolation according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach, Germany) and used immediately in co-culture assays ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were resuspended in binding buffer after centrifuged and sorted via human CD3 MicroBeads (Miltenyi Biotec, #130-050-101) according to the manufacturer’s recommendation (Note ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in 96-well U-bottom plates and blocked prior to staining with 1 μg/mL anti–CD40 (clone HB14, Miltenyi Biotec) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... anti-CD14 PE (Miltenyi Biotec, clone REA599). Surface CD115 (M-CSFR ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were positively isolated using CD14+ microbeads (Miltenyi Biotec) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2022Quote: ... 1G4 T cells were isolated using “untouched isolation” MACS microbead selection (Miltenyi Biotec). Cells were labeled labelled with CTV by mixing 1:1 in PBS ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2022Quote: ... and IL-2 (50 IU/mL; Miltenyi Biotec), and stimulated with Dynabeads (Gibco ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were depleted of CD4 and CD8 using standard MACs magnetic beads (CD4: 130-045-101, CD8: 130-045-201, Miltenyi Biotec, Bergisch Gladbach ...
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bioRxiv - Immunology 2022Quote: ... activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec) and used to determine the replication kinetics of each SCIV.CAM13K Env375 variant (12 ...
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bioRxiv - Immunology 2022Quote: ... Propidium Iodide (Miltenyi Biotec, 130-093-233) or 1:2000 7-AAD (eBiosciences ...
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bioRxiv - Immunology 2022Quote: ... mouse (Miltenyi Biotec, 130-104-075) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The lungs were incubated for 20min at 37 ◦C under continuous rotation and then run on the gentleMACS m-Lung-2 program on the gentleMACS (Miltenyi Biotec, Cat #130-095-937). This was followed by brief centrifugation and the passage of cells through the 70-uM filter ...
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bioRxiv - Developmental Biology 2022Quote: ... and cells were resuspended in 5 mL MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]) and passed through a 40 μm filter (Greiner Bio-One ...
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bioRxiv - Developmental Biology 2022Quote: ... C tubes were placed on a gentleMACS Octo Dissociator with Heaters (Miltenyi Biotec, 130-096-427) and the following protocols were run ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were washed 3x with MACS Buffer (autoMACS Rinsing Solution [Miltenyi Biotec, 130-091-222] with MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376]). After removing supernatant from final wash ...
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bioRxiv - Developmental Biology 2022Quote: ... and lung tissue was finely chopped and transferred to a GentleMACS C tube (Miltenyi Biotec, 130-093-237) (tissue from one mouse per C tube ...
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bioRxiv - Developmental Biology 2022Quote: ... The right ventricle was perfused with ice cold PBS and the lungs were harvested quickly and homogenized and dissociated using the Miltenyi MACS lung dissociation kit (Miltenyi Biotec, cat # 130-095-927), using the GentleMacs program m-Lung-1 on the AUTO MaCs ...
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bioRxiv - Developmental Biology 2022Quote: Muscles were cut into small pieces and transferred to a gentleMACS C tube (Miltenyi Biotec, Bergisch Gladbach, Germany) containing TrypLE Express Enzyme (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The sample was run on a gentleMACS Dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) using the predefined program for murine muscle dissociation ...
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bioRxiv - Genomics 2022Quote: ... were enriched using MicroBead Kit (Miltenyi Biotec) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... T-cells were isolated by Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec) in accordance with the manufacturer’ ss instructions.
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bioRxiv - Immunology 2022Quote: ... Monocytes (CD14+CD16-) were sorted by columns with magnetic beads (Miltenyi Biotec) from total mononuclear cells isolated by Ficoll gradient from the peripheral blood of healthy volunteers ...
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bioRxiv - Immunology 2022Quote: ... Human monocytes were differentiated into a macrophage-like phenotype (MDM, monocyte derived macrophages) using M-CSF (50 ng/mL, Miltenyi Biotec, Bergisch Gladbach, Germany) for 6 days at 37ºC and 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2022Quote: ... Samples were measured by the MACSQuant Analyzer 16 (Miltenyi Biotec, Gladbach) and analyzed by FlowJo® software (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in Fc receptor blocker (Miltenyi Biotec) diluted in PBS/2% BSA and incubated on ice for 10 min ...
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bioRxiv - Immunology 2022Quote: ... ratio were purified by magnetic-activated cell sorting (MACS®) according to the manufacturer’s instructions (NK cell Isolation kit; Miltenyi Biotec). Total RNA from the samples was isolated using TRIzol® reagent following the manufacturer’s isolation protocol (Life Technologies) ...
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bioRxiv - Immunology 2022Quote: ... and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec). The viability for each cell type was determined using a micro flow cytometer with propidium iodide (Moxi Flow ...
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bioRxiv - Immunology 2022Quote: ... and 100IU/ml recombinant human IL-2 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added ...
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bioRxiv - Immunology 2022Quote: ... T cells were purified using the human Naïve Pan T Cell Isolation Kit (Miltenyi Biotec) and NK cells using the human NK Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: ... Autologous naïve T cells and NK cells were purified by magnetic-activated cell sorting (MACS®; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and IL-21 (Miltenyi Biotec, Bergisch Gladbach, Germany) were added at a concentration of 10ng/ml ...
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bioRxiv - Immunology 2022Quote: ... FITC/PE anti-human REAfinity™ TCR Vδ1 (REA173) and APC anti-human REAfinity™ TCR Vδ2 (REA771) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany); PE/Cy7 anti-mouse FasL (MFL3) ...
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bioRxiv - Immunology 2022Quote: ... IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany), IL-18 (R&D Systems ...
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bioRxiv - Immunology 2022Quote: ... γδT cells were washed three times before co-culturing at a ratio of 1:5 with CD8+T cells isolated from tumor-bearing mice using CD8a (Ly-2) microbeads through magnetic activated cell sorting (MACS) (Miltenyi Biotec, Bergisch Gladbach, Germany). Tumor cell lysate (TCL ...
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bioRxiv - Immunology 2022Quote: ... CD43+ cells were depleted using anti-CD43 MicroBeads (Miltenyi Biotec). CD43- splenic B cells were cultured for 3 days at a density of 1×106 cells per mL in RPMI 1640 supplemented in 10% serum calf fetal ...
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bioRxiv - Immunology 2022Quote: ... anti-IgA-Viogreen (Miltenyi Biotec, 130-113-481).