Labshake search
Citations for Miltenyi Biotec :
301 - 350 of 1546 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... T cells were magnetically separated from other splenocytes using the Mouse Pan-T Cell Isolation Kit II (Miltenyi Biotec, Cat# 130-095-130), labeled with CellTrace™ CFSE (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: Naive CD45.1+ OT-II CD4+ T cells were isolated with MACS (Mouse naive CD4+ T cell isolation kit from Miltenyi Biotec, Bergisch Gladbach, Germany) and transferred intravenously into congenic mice (105 cell/mouse) ...
-
bioRxiv - Immunology 2024Quote: CD4+ T cells were prepared from normal BL/6 spleens using a CD4+ T Cell Isolation Kit II for mouse (Miltenyi Biotec, Cambridge, MA) in accordance with the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... then naïve CD4+ T cells (CD4+CD62L+CD44-) were isolated using the Miltenyi Naïve CD4+ T cell isolation kit (Miltenyi Biotec, 130-104-453), and followed by purity check by flow cytometry ...
-
bioRxiv - Immunology 2024Quote: ... naïve CD8+ T cells were purified by magnetic bead separation using mouse naïve CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-096-543) following vendor instructions ...
-
bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched for using the Miltenyi Mouse Naïve CD4+ T cell Purification Kit (Miltenyi Biotec, Cat. # 130-104-453) as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: Primary T cells were sorted from primary PBMC from healthy donors using Pan T cell isolation kit – human (Miltenyi Biotec, # 130-096-535). Isolated T cells were stimulated with plate-bound anti-CD3 and anti-CD28 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: ... The enriched B cells were resuspended in 1 % BSA in PBS x1 and 2 mM EDTA and stained with anti-CD19-FITC (Miltenyi Biotec, 130-113-645), anti-igG-APC (Miltenyi Biotec ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cells were then stained with anti-human CD8-FITC (BW135/80, Miltenyi biotec) and anti-human CD4-PE-Cy7 (SK3 ...
-
bioRxiv - Immunology 2022Quote: ... 4×105 neutrophils were stained with AnnexinV-FITC in Binding Buffer (Miltenyi Biotec). For mitochondria analyses ...
-
bioRxiv - Immunology 2021Quote: ... and stained with FITC-conjugated anti-IFN-γ (clone 45-15, Miltenyi Biotec) and PE-conjugated anti-IL-4 (clone 7A3-3 ...
-
bioRxiv - Immunology 2020Quote: ... PerCPVio770-CD123 (IL-3R) and PE-CD304 (BDCA4) FITC-CD303 (BDCA2) (Miltenyi Biotec). The plate was then incubated at 4 C for 30 minutes followed by addition of 200ul of FACS buffer and centrifugation at 300g for 10 minutes at 4 C ...
-
bioRxiv - Cell Biology 2020Quote: ... CD31-FITC (4μl/sample, Cat nb. 130-110-668, Clone REA730, Miltenyi Biotec), CD31-PerCP-Vio700 (4μl/sample ...
-
Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Ly-76 (TER119) and CD43 (S7) and magnetic anti-FITC MicroBeads (Miltenyi Biotec). Preparations were approximately 95-98% pure for CD19+ B220+ B cells ...
-
bioRxiv - Immunology 2023Quote: ... The labeling mix contained B220-FITC (REA755, Miltenyi Biotec®, Bergisch Gladbach, Germany) and CD3-PE (REA641 ...
-
bioRxiv - Physiology 2024Quote: Anti-CD45-VioBright FITC (12µl/sample, cat. no.: 130-114-567, Miltenyi Biotec)
-
bioRxiv - Neuroscience 2022Quote: ... Cells were then washed in MACS-buffer und T cells were MACS-sorted from suspension using a Pan T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
-
bioRxiv - Bioengineering 2021Quote: ... CD3/CD28-mediated activation of T cells was conducted by using 10 μl of T cell TransActTM (130-111-160; Miltenyi Biotec B.V. & Co. KG) according to manufacturer’s instructions (T cell activation medium) ...
-
bioRxiv - Immunology 2020Quote: ... OT-I CD8+ T cells were then purified by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec: 130-104-075). For cell division or microscopy studies ...
-
bioRxiv - Microbiology 2021Quote: Total spleen T cells were purified by negative selection with the mouse naïve CD4+ T Cells Isolation Kit (Miltenyi Biotec, Cat# 130-104-453) and LS separation columns (Miltenyi Biotec ...
-
bioRxiv - Microbiology 2022Quote: CD4+ T cells were isolated from PBMC population using the CD4+ isolation kit (CD4+ T cell Isolation kit II, Miltenyi Biotec, San Diego, CA). CD4+ T cells were first cultured for three days using stimulating conditions (RPMI supplemented with 10% donor autologous serum ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched CD4+ T cells were stained with fluorescent antibodies against CD4 ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
-
bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
-
bioRxiv - Cancer Biology 2022Quote: ... cryopreserved human PBMCs were thawed and primary human CD3+ T cells were isolated from PBMCs by using human Pan T Cell Isolation Kit (Miltenyi Biotec, 130-096-535). Cells were activated and expanded with Dynabeads at 1:1 ratio ...
-
bioRxiv - Immunology 2023Quote: ... Naïve CD8 T lymphocytes were purified from total PBMC by Naïve CD8+ T Cell Isolation Kit and MACS® separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), following manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... T cells were activated with T cell activation/expansion CD2/3/28 beads at a ratio of 1:2 bead to cells (Miltenyi Biotec). Cultures were maintained in HPLM media with 5% dialyzed FBS (Sigma Aldrich ...
-
bioRxiv - Immunology 2022Quote: ... antibodies in T cell media (RPMI containing 10% FBS, 2mM L-Glutamine, 1% Penicilin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10ng/ml each ...
-
bioRxiv - Immunology 2024Quote: ... Purified CD154+ T cells were expanded in presence of 1:100 autologous antigen-loaded irradiated feeder cells in TexMACS medium (Miltenyi Biotec), supplemented with 5% (v/v ...
-
bioRxiv - Immunology 2023Quote: ... and 1:8 with CD8+ T cells enriched using mouse CD8 (Ly-2) Microbeads kit (Miltenyi Biotec Cat # 130-049-401) and labeled with CellTrace Violet (CTV ...
-
bioRxiv - Biophysics 2023Quote: ... antibodies in T cell media (RPMI 10 % FBS, 2 mM L-Glutamine, 1 % Penicillin-Streptomycin) with IL-15 and IL-7 (Miltenyi Biotec, 10 ng/ml each ...
-
bioRxiv - Plant Biology 2021Quote: ... H-tagged proteins were detected using the anti-HA HRP-coupled antibody (mouse monoclonal, Miltenyi Biotec) diluted 1:5000 (v/v) ...
-
bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched using CD8 MicroBeads (Miltenyi Biotec) following manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... CD8+ T cells were cultured in TexMACS media (Miltenyi Biotec) supplemented with 100 U/ml human IL-2 (Miltenyi Biotec ...
-
bioRxiv - Immunology 2020Quote: The Pan T Cell Isolation Kit II (Miltenyi Biotec, USA) was used for indirect isolation of T cells (CD3+CD8+ cytotoxic T cells and CD3+CD4+ T helper cells ...
-
bioRxiv - Immunology 2022Quote: ... Naive CD4+ T cells (CD62L+ CD44-) were purified (Miltenyi Biotec) using an AutoMACS ...
-
bioRxiv - Immunology 2022Quote: ... using the TCR γδ T cell Isolation kit (Miltenyi Biotec). RNA was extracted using the Nucleospin RNAII kit (Macherey Nagel) ...
-
bioRxiv - Immunology 2024Quote: ... CD8+ T-cells were enriched via positive selection (Miltenyi Biotec), resuspended in TRIzol™ (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2024Quote: ... using a CD4+ T-cell isolation kit from Miltenyi Biotec Inc ...
-
bioRxiv - Immunology 2024Quote: ... or 10μL/mL T-cell TransAct αCD3/αCD28 (Miltenyi Biotec), respectively ...
-
bioRxiv - Cancer Biology 2023Quote: ... depleted from CD3+ T cells using CD3 MicroBeads (Miltenyi Biotec) and 1×106 cells were injected intra-hepatically in 30 µl of PBS ...
-
bioRxiv - Biophysics 2023Quote: ... T cells were cultured in TexMACS GMP Medium (Miltenyi Biotec), which was supplemented with IL7 (155 U/mL) ...
-
bioRxiv - Immunology 2024Quote: ... CD4 T cells were purified (Miltenyi Biotec, #130-094-131). The remaining fraction of naïve T cells was considered as naïve CD8 T cells ...
-
bioRxiv - Immunology 2022Quote: ... an isotype control antibody was used (1:50 dilution, REA control antibody, Miltenyi Biotec, Germany). Samples were analysed using a flow cytometer (Becton Dickinson FACSCalibur) ...
-
bioRxiv - Immunology 2020Quote: ... We magnetically isolated all CD4+ T cells from each sample using a human CD4+ T cell negative isolation kit (Miltenyi biotec, Cat #130-096-533) per manufacturer instructions ...
-
bioRxiv - Immunology 2024Quote: Primary CD4+ T cells were enriched from healthy donor cryopreserved PBMCs by negative selection (CD4+ T Cell Isolation Kit human, Miltenyi Biotec, cat. 130-096-533) and activated for 72h with 10 µg/mL of plate-bound Ultra-LEAF™ purified anti-human CD3 monoclonal antibody (clone OKT3 ...
-
bioRxiv - Plant Biology 2024Quote: ... Proteins were detected using HRP-conjugated anti-GFP (1:5,000; Miltenyi Biotec), anti-plant actin (1:5000 ...
-
bioRxiv - Cancer Biology 2020Quote: Apoptosis was examined by flow cytometry using Annexin V-FITC Kit (Miltenyi Biotec, Italy), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Apoptosis assay was performed using Annexin V-FITC Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...