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Citations for Miltenyi Biotec :
201 - 250 of 1546 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... The naïve T cells were purified from human PBMC using Pan T cell isolation kit (Miltenyi Biotec, Cat#130096535) and activated and expanded by CD3/CD28 Dynabeads (GibcoTM ...
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bioRxiv - Immunology 2023Quote: ... T cells were purified from PBMC by magnetic-bead-based negative T-cell isolation (Miltenyi Biotec, Bergisch Gladbach, Germany) as described before (Wabnitz et al. ...
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bioRxiv - Immunology 2024Quote: ZFP36L2CKO and WT OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting cell suspension was washed twice with PBS and used for the isolation of naïve CD4+ T cells according to the manufacturer’s instructions (Naive T Cell Isolation Kit II; Miltenyi Biotec). Naive CD4+ T cell purity was assessed by staining for CD4 PerCPeFluor710 (clone ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in PBS/FCS and naïve CD4+ T cells were isolated using the naïve CD4+ T cell isolation kit (mouse) from Miltenyi Biotec according to their standard protocol ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were purified using MACS Miltenyi mouse CD8α T Cell Isolation kit (Miltenyi Biotec 130-104-075) before proceeding to single-cell emulsion with 15946 splenic CD8+ T cells and 11856 brain CD8+ T cells ...
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bioRxiv - Genomics 2021Quote: ... CD4+ T cells were then isolated from CD14+ monocyte-free PBMCs using anti-human CD4 antibody attached to microbeads (Miltenyi Biotec #130045101) on a new LS column ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells, (#130-101-638, Miltenyi Biotec for B cells ...
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bioRxiv - Immunology 2023Quote: ... were incubated in 96-well flat-bottom plate and stimulated at a ratio of 2:1 in the presence of NHP-specific MACSiBead™ Particles loaded with biotinylated antibodies to mimic antigen-presenting cells (T Cell Activation/Expansion Kit, Miltenyi Biotec). Responder cells were labeled with cell trace violet prior to incubation ...
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bioRxiv - Immunology 2022Quote: ... both naïve and memory OT-1 CD8 T cell donors were magnetically sorted via magnetic beads (Miltenyi Biotec) with specific antibodies ...
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bioRxiv - Immunology 2021Quote: ... Cells derived from low-density fraction were stained using FITC-labeled anti-EpCAM mAb (dilution: 1/10, HEA-125, Miltenyi Biotec), PE-labeled anti-CD45 mAb (dilution ...
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bioRxiv - Immunology 2021Quote: ... Allogenic naïve CD4+ T cells and CD8+ T cells were isolated from buffycoats using the naïve CD4+ T cell Isolation kit II (Miltenyi Biotec) and CD8+ T cell Isolation kit (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells were purified via negative selection using the Naïve CD4+ T cell Isolation mouse Kit (Miltenyi Biotec) according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: RM CD4+ T cells were isolated from PBMCs using the non-human primate CD4+ T cells isolation kit (Miltenyi Biotec), activated using the NHP T-Cell Activation/Expansion kit (Miltenyi Biotec ...
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bioRxiv - Microbiology 2021Quote: ... Non-T-cells containing monocytes and B-cells were prepared by T-cell depletion using anti-biotin MicroBeads (Miltenyi Biotec), biotin-labeled anti-CD4 mAb (OKT4 ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T-cells were first isolated from HLA-DP4+ asymptomatic carriers using the Pan T-cell Isolation Kit (Miltenyi Biotec) and biotin-labeled anti-CD8 mAb (RPA-T8 ...
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bioRxiv - Immunology 2021Quote: ... Naïve murine CD8+ T cells were isolated using negative selection (Naïve CD8 T Cell Isolation Kit, Cat no. 130-096-543, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... naïve CD4+ T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec) into complete RPMI media ...
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bioRxiv - Immunology 2023Quote: ... or CD45.2+ 4get-GFP OT-II T cells were isolated from LNs and spleens using the naïve CD4+ T cell isolation kit (Miltenyi Biotec). The average purity of OT-II cells was approximately 75-80% for all experiments ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated from PBMC using Human CD4+ T Cell Isolation Kits (Miltenyi Biotec, Inc., Bergisch Gladbach, Germany). Naïve CD4+ T cells were isolated using Human Naïve CD4+ T Cell Isolation Kit II (Miltenyi Biotec) ...
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bioRxiv - Genetics 2024Quote: ... The PBMCs were further processed to isolate human primary T cells using MACS based PAN-T isolation kits (Miltenyi Biotec). RPMI-1640 (Gibco ...
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bioRxiv - Immunology 2024Quote: T-cells were isolated from healthy donor PBMCs using the Pan T-cell Isolation Kit following manufacturer’s instructions (Miltenyi Biotec). Isolated T-cells were activated using anti-CD3/CD28 antibody-coated Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Immunology 2024Quote: ... or Panx1-KO (CD4-Cre Panx1fl/fl) CD8+ T cells were isolated from naïve mice with the Mouse CD8+ T cell isolation kit (Miltenyi Biotec). Cells were stimulated for 3h ...
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bioRxiv - Immunology 2024Quote: ... and negative magnetic selection was then used to purify CD4+ T cells (CD4 T cell isolation Kit, human, Miltenyi Biotec). Isolated CD4+ T cells were plated at a density of 0.8x106/ml in AIM media supplemented with CTS Immune Cell SR (Gibco ...
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bioRxiv - Immunology 2021Quote: ... and CD8+ T cell Isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2020Quote: ... and activated with T Cell TransAct (Miltenyi Biotec); 36-48 h after activation ...
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bioRxiv - Microbiology 2022Quote: ... and T Cell TransAct™ human (Miltenyi Biotec) for 72 h.
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bioRxiv - Immunology 2022Quote: ... and CD4+ T cell isolation kits (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: ... and labelled with CD11b-FITC conjugate (Miltenyi Biotec, Bergisch Gladbach, Germany) for microglial isolation (FACSAria III ...
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bioRxiv - Biochemistry 2021Quote: ... and labeled with 0.6 µL anti-c-myc-FITC (Miltenyi Biotec) and 0.25 µL Goat anti-Human IgG Fc PE conjugate (ThermoFisher #12-4998-82) ...
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bioRxiv - Bioengineering 2024Quote: ... reactions were labeled with 0.6μl anti-c-myc-FITC (Miltenyi Biotec), 0.25μl SAPE (streptavidin-R-phycoerythrin ...
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bioRxiv - Bioengineering 2024Quote: ... reactions were labeled with 12μl anti-c-myc-FITC (Miltenyi Biotec), 5μl SAPE (streptavidin-R-phycoerythrin ...
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bioRxiv - Cancer Biology 2022Quote: ... and with anti-CD45-FITC (Miltenyi Biotec, Cat.130-110-658). EdU incorporation was detected using the Click-iT Plus EdU Flow Cytometry Assay Kit according to the manufacturer’s instructions (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... the expression of Ki-67 (anti-Ki-67-FITC, Miltenyi Biotec, clone ...
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bioRxiv - Immunology 2024Quote: ... and (iii) anti-NKG2A-VioBright FITC (clone REA110) from Miltenyi Biotec. Cytosolic/intranuclear expression of Ki67 was detected using anti-Ki67–BV421 (clone B56 ...
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bioRxiv - Immunology 2020Quote: ... Nylon wool-enriched splenic T cells from naive or immunized mice were incubated sequentially for 10 min at 4°C with FITC-conjugated anti-mouse γδ TCR or αβ TCR Abs and 15 min at 4°C with anti-FITC Microbeads (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany), then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH) ...
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bioRxiv - Immunology 2021Quote: ... and purified their T cells using the untouched isolation kit (Pan T Cell Isolation Kit II, mouse, 130-095-130, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Human CD4+ T-cells were isolated from peripheral blood lymphocytes using naïve CD4+ T-cell isolation kit from Miltenyi Biotec (Germany). The blood samples were obtained from Rheumatology Clinic at Saint Louis University with informed consent ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were processed as previously described 54 and CD4+ T cells were enriched with the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... density centrifugation and primary CD4+ T cells were purified by negative selection of CD4+ cells using magnetic beads (CD4+ T cell isolation kit, human; Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Primary human T regulatory cells (Tregs) were isolated from PBMCs using CD4+ CD25+ CD127dim/- Regulatory T Cell Isolation Kit (Miltenyi Biotec). The isolated Treg cells were expanded in culture for 14 days with TexMACS medium (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Peripheral blood mononuclear cells were isolated by ficoll density gradient centrifugation (Dakewe) and T cells were purified using the Pan T Cell Isolation Kit (Miltenyi Biotec), and stimulated with CD3/CD28 T cell Activator Dynabeads (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... The CD8+ T cells were purified from PBMCs using negative selection bead binding (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec). The following compounds were used at the indicated concentrations ...
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bioRxiv - Microbiology 2020Quote: ... CD4+ T cells were obtained via negative selection from PBMCs using a human CD4+ T-cell isolation kit (MACS Miltenyi Biotec, #130-096-533 ...
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bioRxiv - Immunology 2020Quote: Cryogenically preserved healthy human peripheral blood T-cells or murine splenic T-cells were magnetically isolated by negative selection using Pan T-cell isolation kits from Miltenyi Biotec. Murine splenocytes underwent RBC lysis (Sigma ...
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bioRxiv - Immunology 2020Quote: ... lymphocytes were collected from spleens and lymph nodes of naïve CD45.1+ and CD45.1.2+ P14 TCR transgenic mice and negatively enriched for CD8+ T cells using the CD8a+ T Cell Isolation Kit and LS MACS Columns (Miltenyi Biotec). 1×106 CD8+ T cells were plated per well in 48 well flat-bottom plates ...
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bioRxiv - Immunology 2022Quote: ... We then purified CD4 CD25 and CD4+CD25− T cells using CD4+CD25+ Regulatory T Cell Isolation Kit (#130-091-301) and AutoMACS technology (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... PBMC were isolated from a Buffy Coat from healthy volunteer donors using Ficoll gradient centrifugation and human T cells were isolated using the Human Pan T cell isolation kit (Miltenyi Biotec). Human T cells were cultured in supplemented RPMI media as described for cell lines above ...
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bioRxiv - Microbiology 2021Quote: ... 1x105 HEK-293T cells were mixed at a 1:1 ratio in 96-well plates with primary CD4+ T lymphocytes freshly isolated from healthy donors by negative selection (CD4+ T-Cell Isolation Kit II, human, Miltenyi Biotec). Viral transfer was assessed after 24 hours of incubation at 37°C in permeabilized (FIX & PERM Cell Permeabilization kit ...