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Citations for Miltenyi Biotec :
251 - 300 of 1546 citations for T complex protein 1 subunit epsilon CCT5 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... After MACS sorting of CD8+ T cell using a negative selection kit (CD8+ T Cell Isolation Kit II, human, Miltenyi Biotec), cells were lysed for western blotting or stained for Autophagy flux assay as described below ...
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bioRxiv - Immunology 2020Quote: ... anti-PDL1-PE/Cy7 and anti-FasL-APC to analyze surface markers on B cells involved in T cell suppression and with CD4-APC-Vio770 to investigate T cell proliferation by flow cytometry (MACSQuant Analyzer 10, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... PolyPeptide group) were co-cultured with 105 OTII CD4+ T cells (purified with a CD4+ T cell isolation kit, Miltenyi Biotec) in RPMI medium (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... CD8+ T cell from spleen cell suspension were isolated using mouse CD8a+ T Cell Isolation Kit (Miltenyi Biotec; 130-104-075) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... T cells from spleens of 4 weeks LmCen-/- immunized mice were isolated using mouse Pan T cell isolation kit (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: CD8+ T cells obtained using Ficoll density gradient centrifugation (Lymphoprep, ELITechGroup) followed by CD8+ T cell isolation (CD8+ T Cell Isolation Kit, human, Miltenyi Biotec) on buffy coats from HLA-A2.1+ human donors after written informed consent in accordance with the Declaration of Helsinki and in agreement with institutional guidelines ...
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bioRxiv - Immunology 2024Quote: Single cells suspension from tumour-bearing lungs described previously were used for CD8+ T cells sorting using immunomagnetic sorting using CD8α+ T cell isolation kit (Miltenyi Biotec) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: Isolation of untouched CD8+ T cells from total PBMCs was performed using a two-step human CD8+ T cell isolation kit (Miltenyi Biotec). All steps were performed following manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... OT-I CD8 T (OT-I) cells were enriched by negative selection using a CD8a+ T cell isolation kit (Miltenyi Biotec), commonly reaching a purity of 90-98% as determined by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the minced auricular lymph nodes using the CD4+ T cell isolation kit (Miltenyi Biotec) and cultured in RPMI 1640 media supplemented with 25 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... Autologous CD4+ T cells were isolated from PBMCs with a negative CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... OT-I T cells were enriched by magnetic activated cell sorting (MACS) using the CD8a+ T Cell Isolation Kit (Miltenyi Biotec) or using the EasySepTM Mouse CD8+ T Cell Isolation Kit (Stem Cell Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... remaining cells were processed for T cell purification using the Pan T cell isolation kit (Miltenyi Biotec, cat # 130-095-130). Cell viability of the purified T cells was ascertained by labeling with the eBioscience™ Fixable Viability Dye eFluor™ 506 for 30 min on ice ...
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bioRxiv - Synthetic Biology 2023Quote: ... were isolated using Ficoll gradient centrifugation and human T cells were obtained by magnetic sorting using the eHuman Pan T cell isolation kit (Miltenyi Biotec). Once isolated ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from wildtype mice spleens using CD8a+ T Cell Isolation Kit (130-104-075) from Miltenyi Biotec according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Human CD8+ T cells were isolated from healthy donors via magnetic bead labeling (CD8+ T cell isolation kit, Miltenyi Biotec, Germany). U937 (ATCC ...
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bioRxiv - Immunology 2024Quote: ... was used to lyse red blood cells before T cell isolation using the naive CD4+ T Cell Isolation Kit from Miltenyi Biotec or the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kits from BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... were isolated from healthy donors by Ficoll gradient followed by primary CD4+ T-cell isolation by negative selection and magnetic separation using the human CD4+ T Cell Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Murine T cells were isolated from C57Bl6/J (WT or IFNγ-/-) splenocytes with CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: PBMCs were isolated from whole blood by the ficoll density gradient method (CL5020) and CD4+ T cells were selected using the human CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD8 T cells were isolated from spleens of OT-1 mice using the CD8 mouse isolation kit (Miltenyi Biotec) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2021Quote: CD4+ T cells were separated from healthy controls PBMC using pan T cell isolation kit and magnetic-activated cell sorting (Miltenyi Biotec, USA). Enriched T cells were subsequently stained with CD25-PE mAb and CD25+ T cells positively selected using anti-PE magnetic beads and MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated from splenocytes either by using the CD4+ or CD8+ T cell isolation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s recommendation alone or followed by anti-CD4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3+ T cells were magnetically separated by negative selection with a Human Pan T cell isolation Kit (#130-096-535, Miltenyi Biotec, Germany) and stored long-term in Bambanker serum-free cell freezing medium (#BB01 ...
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bioRxiv - Physiology 2023Quote: ... CD4+ T cells were then purified from the isolated PBMCs by negative selection using humanCD4+ T cell isolation kits (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Mouse CD4+ T cells were purified by negative selection using a CD4+ T cell isolation kit (Miltenyi Biotec, cat. # 130-104-454). For CD8 depletion ...
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bioRxiv - Immunology 2024Quote: ... wild-type CD4+ T cells were isolated using the Miltenyi Mouse CD4+ T cell isolation kit (Miltenyi Biotec, Cat. # 130-104-454) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: CD4+ naïve T cells were purified using the naïve CD4+ T cell Isolation kit for mouse from Miltenyi Biotec (130-104-453). For short term kinetics 10×106/ml naïve CD4 T cells were stimulated with 1µg/ml of α- CD3 and α-CD28 for the indicated time points ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells and naïve CD8a+ T cells were enriched using either a naïve CD4 isolation kit (Miltenyi Biotec, 130-104-453) or naïve CD8a isolation kit (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2021Quote: ... αβ T cells were transduced with pMP71: γ-T2A-δ and incubated with a biotin-labelled anti-αβ TCR antibody (clone BW242/412; Miltenyi Biotec) followed by incubation with an anti-biotin antibody coupled to magnetic beads (anti-biotin MicroBeads ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with AnnexinV-FITC and CD4 APC (Miltenyi Biotec, Italy) for 15 minutes in the dark at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by washing and incubation with anti-FITC MACS microbeads (Miltenyi Biotec) for 15 minutes at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with an CD137-VioBright-FITC mAb (REA765, Miltenyi Biotec) and subsequently with anti-FITC microbeads and enriched by magnetic isolation using LS MACS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2020Quote: CD8+ T cells were isolated by MACS (Miltenyi Biotec) from draining inguinal lymph nodes of MB49 bearing male and female mice 14 days post implantation (Fig ...
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bioRxiv - Immunology 2022Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... T cells were negatively selected by MACS (Miltenyi Biotec) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... CD4+ T Cell Isolation Kit was from Miltenyi Biotec Ltd. ...
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bioRxiv - Immunology 2021Quote: ... or Human CD8+ T Cell Isolation Kit (Miltenyi Biotec), stimulated with Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... using the CD4+ T Cell Isolation Kit (Miltenyi Biotec) and were cultured in RPMI medium containing 10% FBS ...
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bioRxiv - Immunology 2024Quote: CD8+ T cells positively sorted by MACS (Miltenyi Biotec) were assessed for viability by AO/PI staining and counted with a Luna-FX7TM Automated cell counter (Logos Biosystems).
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by MACs (Miltenyi Biotec) from the spleens of naïve mice ...
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bioRxiv - Immunology 2023Quote: ... T cells were depleted using CD90.2 microbeads (Miltenyi Biotec). Mice were maintained on 0.03% enrofloxacin in drinking water for up to 1 wk before and for 2 wk after irradiation and then were housed in autoclaved cages with sterile water ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were enriched by MACS (Miltenyi Biotec) and CD40L+4-1BB+ and CD40L-4-1BB- cells FACS sorted using an Aria SORP (BD) ...
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bioRxiv - Cancer Biology 2024Quote: ... then used Pan T cell isolation kit (Miltenyi Biotec) to isolate T cells ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies were captured with 100µl of μMACS protein A beads (Miltenyi Biotec; San Diego, CA). Beads were isolated with μ columns and a μMACS separator (Miltenyi Biotec ...
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bioRxiv - Bioengineering 2022Quote: ... for the cardiac marker Cardiac troponin T conjugated to APC (cTnT; 1:50; REA400; 130-120-543, Miltenyi Biotec, Germany), and cells were frozen in RPMI + B27C with 10 µM of RI + 10% FCS + 10% DMSO ...
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bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).