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Citations for Miltenyi Biotec :
3201 - 3250 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were obtained from the lymph nodes (LNs) and spleens of OT-1 mice using a negative selection kit (130-104-075, Miltenyi Biotec). For CTL differentiation ...
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bioRxiv - Immunology 2023Quote: ... the cells were washed with PBS and resuspended in 50 µl of 1X annexin binding buffer containing 2 µl of FITC-annexin V (Miltenyi Biotec, 130-092-052). Cells were then incubated at room temperature for 30 min in the dark ...
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bioRxiv - Microbiology 2023Quote: ... sorbitol-synchronized iRBCs were transferred to MACS cell separation LD columns (Miltenyi Biotec, Bergisch Gladbach, Germany), and washed and eluted in CM ...
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bioRxiv - Immunology 2023Quote: ... Cytotoxic T cells and T helper cells were isolated from the PBMCs by positive selection with CD8- and CD4-specific MicroBeads (Miltenyi Biotec), respectively ...
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bioRxiv - Immunology 2023Quote: ... MACSQuant Analyzer (Miltenyi Biotec) or a Cytek Aurora Analyzer (Cytek) ...
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bioRxiv - Immunology 2023Quote: ... and 50 ng/mL of M-CSF (Miltenyi Biotec) for 5 days ...
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bioRxiv - Microbiology 2023Quote: ... Enrichment of primary CD4+ T cells was performed using the EasySep™ Human CD4+ T Cell Enrichment Kit (Miltenyi Biotec) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2023Quote: ... The remaining supernatants were adjusted so that they had the same amount of total protein and mixed with 25 μL α-FLAG magnetic bead as instructed in the μMACS Epitope Tag Protein Isolation Kit (Miltenyi Biotec). From here ...
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bioRxiv - Microbiology 2023Quote: ... right lung lobes were transferred into gentleMACS C tubes (Miltenyi Biotec) containing 2.5mL pre-warmed digestion buffer consisting of filter-sterilized PBS (with calcium and magnesium ...
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bioRxiv - Microbiology 2023Quote: ... Lungs were dissociated in a gentleMACS Dissociator (Miltenyi Biotec), followed by incubation at 37°C for 45min ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant TGF-β1 50 ng/mL (Miltenyi Biotec).
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bioRxiv - Neuroscience 2023Quote: ... CD11b-positive brain cells were selected from the brain with microbeads-conjugated CD11b antibody (clone M1/70.15.11.5; Miltenyi Biotec) and CD4-positive spleen cells from the spleen with Dynabeads magnetic beads-conjugated CD4 antibody (clone L3T4 ...
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bioRxiv - Neuroscience 2023Quote: ... respectively (Miltenyi Biotec GmbH, Bergisch Gladbach, Germany). After blocking with 50 µg/ml CD16/CD32 antibody (clone 2.4G2 ...
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bioRxiv - Neuroscience 2023Quote: ... At d15 cells were treated with IL-4 (20 ng/ml, LOT130-094-117, Miltenyi Biotec), IL-6 (100 ng/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 ng/ml IL-7 (#130-093-937, Miltenyi Biotec) and 3 ng/ml IL-15 (#130-093-955 ...
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bioRxiv - Molecular Biology 2023Quote: ... natural killer cells and monocytes were isolated from PBMCs by negative selection and magnetic sorting using cocktails of biotin-conjugated antibodies against non-target cells (#130-096-535,Miltenyi Biotec for T cells ...
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bioRxiv - Molecular Biology 2023Quote: ... (#130-101-638, Miltenyi Biotec for B cells, #130-100-777, Miltenyi Biotec for dendritic cells, #130-092-657, Miltenyi Biotec for natural killer cells ...
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bioRxiv - Microbiology 2023Quote: ... Cells were stimulated with IL-2 (10 ng/ml) (Miltenyi Biotec #130-097-745) and with anti-CD3/CD28 beads (Gibco #11132D ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were analysed on a MACSQuant Analyzer flow cytometer (Miltenyi Biotec) and data analyzed using FlowJo (TreeStar) ...
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bioRxiv - Molecular Biology 2023Quote: ... CD34+ HSPCs were isolated from GCSF mobilized healthy donor apheresis using CD34+ Microbead kit (Miltenyi Biotec, UK). The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... and 100 ng/ml TNF (130-094-014, Miltenyi Biotec). In 24-well plates ...
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bioRxiv - Microbiology 2023Quote: ... Blood neutrophils were separated by negative magnetic selection (MACSxpress, Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and residual erythrocytes were further removed using the MACSxpress Erythrocyte Depletion kit (Miltenyi Biotec, Germany). The resulting neutrophil suspension was washed with HBSS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 μL of μMACS anti-GFP or anti-c-Myc (Miltenyi Biotec, 130-091-125 and 130-091-123) was added to clarified extract and incubated at 4 °C while rotating for 2 hours ...
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bioRxiv - Microbiology 2023Quote: ... immune cells from the colonic lamina propria were extracted using the Lamina Propria Dissociation Kit for mice and gentleMACS dissociator (Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Cells were passed through LS columns affixed to MACS magnetic separator (Miltenyi Biotec). Bound fractions were eluted with FACS buffer.
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... naive CD4+ T cells (CD4+CD25−CD62L+) were sorted from the spleen of WT C57Bl/6 mice using a commercially available kit (Miltenyi Biotec). Next ...
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bioRxiv - Genomics 2023Quote: ... Multi Tissue Dissociation Kit 1 and gentleMACS Octo Dissociator (Miltenyi Biotec) were used for dissociation of the tissue into single-cell suspension with a reduced amount of enzyme R (25% of the regular amount) ...
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bioRxiv - Genomics 2023Quote: ... the dissociated single-cell suspensions were thawed and filtered using a 70 μm cell strainer (Miltenyi Biotec) to remove debris ...
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bioRxiv - Genomics 2023Quote: ... Red blood cells (RBC) were lysed using Red Blood Cell Lysis Solution (catalog no. 130-094-183, Miltenyi BIotec) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... provided in the Adult Brain Dissociation kit (#130-107-677, Miltenyi Biotec). Briefly ...
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bioRxiv - Genomics 2023Quote: ... with all reagents included in the Adult Brain Dissociation kit (#130-107-677, Miltenyi Biotec). Transcription and translation inhibitors were included during cell preparation to prevent ex vivo activational artifacts ...
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bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 1 ml 0.5% BSA (#130-091-376, Miltenyi Biotec) in D-PBS and filtered through a 35-μm filter (#352235 ...
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bioRxiv - Genomics 2023Quote: ... Cells were stained with CD11b-APC (M1/70, #130-113-793, Miltenyi Biotec) and CD45-VioBlue (REA737 ...
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bioRxiv - Genomics 2023Quote: ... and placed into ice-cold gentleMACS C-tubes (#130-093-237, Miltenyi Biotec), containing 1950Lμl of Enzyme Mix 1 ...
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bioRxiv - Genomics 2023Quote: ... and CD45-VioBlue (REA737, #130-110-802, Miltenyi Biotec). Following staining ...
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bioRxiv - Genomics 2023Quote: ... Each sample had 30Lμl of Enzyme Mix 2 added before being mechanically dissociated for 30Lmin at 37°C on the gentleMACS Octo Dissociator with Heaters (#130-096-427, Miltenyi Biotec) using the 37C_ABDK_02 program ...
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bioRxiv - Genomics 2023Quote: ... passed through a pre-wet 70Lμm MACS SmartStrainer (#130-110-916, Miltenyi Biotec), and collected in a 50-ml conical tube (#21008-178 ...
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bioRxiv - Immunology 2023Quote: ... A commercially available CytoBox TH17 mouse cocktail (130-107-758, Miltenyi Biotec, USA) was used for polarization of murine CD4+ T cells in-vitro ...
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bioRxiv - Immunology 2023Quote: ... four subsets in CD8+CD45RClow/- Tregs were labelled and sorted according to the expression of TNFR2+/- (Miltenyi Biotec, REA520) and CD29low/high (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... and CD29low/high (Miltenyi Biotec, REA1060). Purity was greater than 95%.
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bioRxiv - Immunology 2023Quote: ... the cells were incubated with 10µL of Streptavidin Microbeads (Miltenyi Biotec) per 107 cells for 15 minutes on ice and then washed once with FWB+EDTA ...
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bioRxiv - Genomics 2023Quote: ... Lineage cells were depleted using Mouse Lineage Cell Depletion Kit (catalog no. 130-110-470, Miltenyi BIotec) and pass through LD depletion columns (catalog no ...
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bioRxiv - Genomics 2023Quote: ... Live cell enrichment was performed using MACS Dead Cell Removal Kit (Miltenyi Biotec, cat#: 130-090-101) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... the supernatant was aspirated and debris was removed using the Debris Removal solution (#130-109-398, Miltenyi Biotec) provided in the Adult Brain Dissociation kit (#130-107-677 ...
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bioRxiv - Genomics 2023Quote: ... a magnetic negative selection was performed using CD235a Microbeads (Miltenyi Biotec, cat#: 130-050- 501) and MACS LS columns (Miltenyi Biotec ...
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bioRxiv - Genomics 2023Quote: ... cells were rinsed 1X with PBS and 1X with autoMACS running buffer (MACS Separation Buffer (130-091-221, Miltenyi Biotec, San Diego, CA, USA). The cardiac Troponin T (TNNT2 ...
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bioRxiv - Immunology 2023Quote: ... CD11c+ cells were enriched using CD11c MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... using CD11c MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol.