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Citations for Miltenyi Biotec :
3001 - 3050 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... cells were stained with APC-labeled mMR1-tet and mMR1-tet positive cells were isolated with anti-APC microbeads and LS columns (Miltenyi Biotec). The isolated MAIT cells were intraperitoneally (IP ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... and 4% BSA in HBSS) was added per tissue and tissues were further homogenized with the GentleMACS dissociator (Miltenyi Biotec) using the following program ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were then labelled with the human NK Cell Isolation Kit (Miltenyi Biotec), with subsequent NK cell isolation using the “depletes” program on an autoMACS Pro Separator and collecting the negative fraction ...
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bioRxiv - Immunology 2023Quote: ... and dying cells (Miltenyi Biotec) and the mix was set on a magnet to elute only living cells ...
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bioRxiv - Immunology 2023Quote: ... Bead-bound cells were positively selected through a magnet-mounted MACS LS column (Miltenyi Biotec; 130-042-401). In some experiments ...
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bioRxiv - Immunology 2023Quote: ... and a MidiMACS™ Separator (Miltenyi Biotec). Switched memory B cells were processed into single cell suspension for 10x single cell immune profiling (5’ gene expression + BCR) ...
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bioRxiv - Immunology 2023Quote: ... or anti-APC microbeads (Miltenyi Biotec; 130-090-855) for 15 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... injected with 3 μg of anti-mouse CD45-FITC antibodies (Miltenyi Biotec, Germany) retro-orbitally and left for 3 min prior to euthanasia by cardiac puncture ...
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bioRxiv - Immunology 2023Quote: ... The livers were harvested and dissociated into single-cell suspensions using the Liver Dissociation Kit on a GentleMACS Octo Dissociator (Miltenyi Biotec). The cell suspension was filtered through a 70 μm strainer ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 25 µl anti-PE (Miltenyi Biotec; 130-105-639) or anti-APC microbeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... NK cells were isolated from fresh blood of healthy donors using the MACSxpress NK Isolation Kit (Miltenyi Biotec, cat. no. 130-098-185). Target cells were incubated with titrated concentrations of mAbs for 10 min and then with primary human NK cells at an effector to target ratio ranging from 7.75:1 to 9:1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Monocytes were labeled using StraightFrom Whole Blood CD14 MicroBeads (Miltenyi Biotec) and purified using an autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... and purified using an autoMACS Pro Separator (Miltenyi Biotec). Monocyte were then cultured in IMDM (Thermo Fisher ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... tissues were placed into a GentleMACS C-tube (Miltenyi Biotec) and cut into approximately 5-mm3 pieces ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotec) or the AttuneNxT acoustic focusing cytometer (4 lasers ...
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bioRxiv - Cancer Biology 2023Quote: ... Human T Cell TransAct (Miltenyi Biotec t# 130-111-160) was used to activate T cells ...
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bioRxiv - Cancer Biology 2023Quote: ... containing IL-2 (Miltenyi Biotec, # 130-097-748), human serum AB (Seralab ...
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bioRxiv - Cancer Biology 2023Quote: ... using human tumor dissociation kit (Miltenyi Biotec Cat. No. 130-095-929) for subsequent experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... and dissociated with a gentleMACS Dissociator (Miltenyi Biotec) using human tumor dissociation kit (Miltenyi Biotec Cat ...
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bioRxiv - Cancer Biology 2023Quote: ... and an enzymatic cocktail (supplementary table 7) and digested at 37°C for 42 minutes (37C_m_TDK_2 program) on a gentleMACS Octo Dissociator (Miltenyi Biotec, 130-096-427). Cell suspensions were then filtered through a 40 μm cell strainer (Corning ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were removed using MACS® Dead Cell Removal Kit (Miltenyi Biotec Inc.). Single-cell cDNA library was prepared and sequenced at the University of Michigan Sequencing Core using the 10x Genomics ...
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bioRxiv - Immunology 2023Quote: ... Tissue pieces were homogenized in 1mL sterile PBS using the gentleMACS Dissociator (Miltenyi Biotec), then homogenates were serially diluted and plated in triplicate on LB agar plates incubated overnight at 37°C ...
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bioRxiv - Immunology 2023Quote: ... CD45+ cells were isolated by CD45 magnetic beads isolation kit (130-052-301, Miltenyi Biotec).
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bioRxiv - Cell Biology 2023Quote: ... After stopping the lysis with 1X PBS+10%FCS, cells were pelleted by centrifugation (300 x g, 5 min, 4°C) and subjected to dead cell removal (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Fibroblasts for RT-PCR and Western blot analyses were negatively sorted on Cd11b microbeads and positively sorted for anti-feeder microbeads through LS columns on a QuadroMACS magnet (Miltenyi Biotec). Fibroblasts for RNASeq ...
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bioRxiv - Bioengineering 2023Quote: CD14 microbeads (Miltenyi Biotec, Cat. # 130-050-201) were used to isolate CD14+ cells from peripheral blood mononuclear cells (PBMCs ...
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bioRxiv - Bioengineering 2023Quote: ... human (Miltenyi Biotec, Cat. # 130-096-535) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... human (Miltenyi Biotec, Cat. # 130-097-095) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Media supplements tested were FGF-2 at a concentration of 10 ng/ml (Human FGF-2 IS premium grade, MACS Miltenyi Biotec, Germany, 130-104-922), EGF at a concentration of 10 ng/ml (Recombinant Human EGF ...
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bioRxiv - Bioengineering 2023Quote: ... The Pan Monocyte Isolation Kit (130-096-537, Miltenyi Biotec, Leiden, The Netherlands) and LS columns (130-042-401 ...
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bioRxiv - Bioengineering 2023Quote: ... and LS columns (130-042-401, Miltenyi Biotec) were used according to the manufacturer’s protocol ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... Cells were resuspended in 80 μL of buffer and 20 μL of Anti-biotin IgG microbeads up to 1×107 cells (Miltenyi Biotec) and incubated at room temperature for 15 minutes ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... and FITC-Labeling Reagent (Miltenyi Biotec, Cat # 327806, 1:50 dilution). Composition analysis of enriched iSCs was performed using anti- ITGB4 (BD Cat # 744150 ...
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A scalable, GMP-compatible, autologous organotypic cell therapy for Dystrophic Epidermolysis BullosabioRxiv - Bioengineering 2023Quote: ... The labeled cell suspension was MACS separated using AutoMACS (Miltenyi Biotec) PosselD program setting ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was then decanted over a QuadroMACS™ LS column (Miltenyi Biotec, 130-042-976) that had previously been equilibrated with 0.5 % BSA in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... and later monocytes were enriched from the total bone marrow cells using the Monocyte Isolation Kit (BM) from Miltenyi Biotec (Cat: 130-100-629) through a negative selection process following the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... according to the manufacturer’s protocol using LS columns (Miltenyi Biotec). CD34+ cells were co-cultured on 80% confluent OP9-DL4 cells in αMEM medium ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry analysis were conducted using BD LSR Fortessa™ cell analyzer or MACSQuant® analyzer (Miltenyi Biotec). Data were prepared using FlowJo 10.1 (Tree Star ...
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bioRxiv - Cancer Biology 2023Quote: ... Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694, Miltenyi Biotec). Cell viability was determined using flow cytometry and 7-AAD viability staining solution (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec). The digested PDX was filtered through a 70 μm cell strainer and then depleted of residual red blood cells using 1X RBC Lysis Buffer (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: ... Dead cells were removed from the digested PDX using a dead cell removal kit (130-090-101, Miltenyi Biotec). Murine cells were removed from the dead-cell-depleted fraction using a mouse cell depletion kit (130-104-694 ...
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bioRxiv - Cancer Biology 2023Quote: ... tumors were extracted in a laminar flow hood under sterile conditions and were immediately digested using the human tumor dissociation kit (130-095-929, Miltenyi Biotec) in combination with the GentleMACS Octo Dissociator (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD3 (Miltenyi Biotec, #130-113-141), CD8 (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2023Quote: Primary human inv(16) AML CD34+ cells were T-cell depleted using CD3 Microbeads (Miltenyi Biotec, #130-050-101) and nucleofected with the RNP complex ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8 (Miltenyi Biotec, #130-110-681), and IFN-γ (BD Pharmingen ...
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bioRxiv - Cancer Biology 2023Quote: ... and CD34+ cells were isolated using the CD34 Microbead kit (Miltenyi Biotec, #130-046-702). The use of the cord blood samples for research purposes was approved by the Ethics Committee of the Medical College of Wisconsin ...
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bioRxiv - Cancer Biology 2023Quote: ... CD34+ cells were enriched using CD34 MicroBead Kit (Miltenyi Biotec, #130-046-702) and cultured overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... and dead cells were eliminated by dead cell removal kit (Miltenyi Biotec, #130-090-101).
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bioRxiv - Cancer Biology 2023Quote: ... followed by dead cell removal by dead cell removal kit (Miltenyi Biotec, #130-090-101). Cells were treated with DMSO/ AI-10-49 (5 µM ...
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bioRxiv - Cell Biology 2023Quote: ... using mouse Pan dendritic cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec)(Vremec and Segura ...