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Citations for Miltenyi Biotec :
3301 - 3350 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and the appropriate programs were run according to the mouse tumor dissociation kit protocol and mouse lung dissociation kit protocol (both from Miltenyi Biotec) for tumors and lungs respectively ...
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bioRxiv - Immunology 2023Quote: ... cut into small pieces using scissors and placed in a gentleMACS C tube (Miltenyi Biotec) in 5 ml RPMI1640 containing 100 μl 10X collagenase/hyaluronidase enzyme mixture (cat ...
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bioRxiv - Immunology 2023Quote: ... Monocytes were isolated from PBMC using CD14 Microbeads (Miltenyi Biotec) and differentiated into inflammatory macrophages using TPP conditions17 ...
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bioRxiv - Immunology 2023Quote: ... and monocytes were positively selected using CD14 Microbeads (Miltenyi Biotec). Macrophage differentiation was performed using conditions that model chronic inflammation (TPP)17 ...
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bioRxiv - Immunology 2023Quote: ... Lungs were then dissociated for 20 minutes using a custom GentleMACS protocols before filtering through a 70 µm Macs SmartStrainer (Miltenyi Biotec, 130-110-916) into Advanced DMEM/F-12 supplemented with 2% FBS (DMEM-FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... was sliced into small pieces and transferred to customer-specified tubes for magnetic-activated cell sorting (MACS) (Adult Brain Dissociation Kit #130-107-677 and Cd11b MicroBeads, mouse #130-093-634, Miltenyi Biotec, Bergisch Gladbach Germany). Samples were processed per manufacturer’s instructions with the following modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... Lung tissue was transferred to gentleMACS C-tubes (Miltenyi Biotec) containing 5 ml of freshly prepared lung digestion medium ...
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bioRxiv - Neuroscience 2023Quote: ... treated with blocking buffer containing BSA (Miltenyi Biotec, Cat# 130-091-376) and the ribonuclease inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... endothelial cells were purified using a CD31 MicroBead Kit (Miltenyi Biotec, 130-091-935) and further cultured in the Promocell endothelial cell medium (MV2 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then placed in C Tubes (Miltenyi Biotec, 130-093-237), along with dissociation reagents from the MACS Neural Tissue Dissociation Kit (P ...
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bioRxiv - Genomics 2023Quote: ... The tissues were digested with Human Tumor Dissociation Kit (Miltenyi Biotec) according to manufacturer’s manual ...
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bioRxiv - Cell Biology 2023Quote: ... and the acquisition of the different samples was performed on MACSQuant (Miltenyi Biotec) flow-cytometer and analysis was completed using FlowJo Software.
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bioRxiv - Cell Biology 2023Quote: ... incubated with CD45+ magnetic microbeads (Miltenyi Biotec, Cat# 130-052-301) and passed through MS Columns (Miltenyi Biotec ...
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bioRxiv - Synthetic Biology 2023Quote: ... anti-CD63 (Miltenyi Biotec, clone H5C6) and anti-CD81 antibodies (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... for microglia (Miltenyi Biotec, Cologne, Germany), for 15 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... enzymatic cell dissociation was performed using a neural tissue dissociation Kit (Miltenyi Biotec, Cologne, Germany). Then ...
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bioRxiv - Systems Biology 2023Quote: ... Data were collected with MACSQuant analyzer (Miltenyi Biotec). Quantification and analysis of the data were done with Flowjo software.
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bioRxiv - Bioengineering 2023Quote: ... the evacuated marrow was transferred to a conical tube through 30 µm pre separation filter (Miltenyi Biotec) to collect the marrow cells.
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bioRxiv - Bioengineering 2023Quote: ... CD61+ megakaryoblasts were enriched from the culture via MACS using anti-CD61 magnetic microbeads (Miltenyi Biotec), and the selected cells were re-cultured into flasks containing IMDM ...
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bioRxiv - Bioengineering 2023Quote: ... before cells were incubated in anti-mouse IgG 2a+b microbeads (Miltenyi Biotec) for 20 min at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... mouse (Miltenyi Biotec, 130-090-862) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were stained with mouse anti-human CD19 antibody (Miltenyi Biotec, cat# 130-113-730; 1:50) in the dark for 25 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed twice and resuspended in 1mL of sorting buffer (up to 10⁸ cells) prior to magnetic separation on LS columns and the MACS Separator (Miltenyi Biotec). Columns were rinsed with 0.5mL of MACS buffer prior to loading the cell suspension ...
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bioRxiv - Bioengineering 2023Quote: ... cells were resuspended in 300 µL of sorting buffer per 107 cells and anti-PE MicroBeads (Miltenyi Biotec) for 20 min at 4°C with constant agitation ...
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bioRxiv - Cell Biology 2023Quote: The following primary antibodies were used for WB in this study: mouse anti-GFP HRP conjugate antibody (Miltenyi Biotec 130-091-833, 1:1000), rabbit anti-golgin-45 (ab155510 ...
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bioRxiv - Cell Biology 2023Quote: ... Pan B cells were isolated from peripheral blood leukocytes using the Pan B cell isolation kit according to manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Cell Biology 2023Quote: ... Samples were analysed using a MACSQuant flow cytometer (Miltenyi Biotec) at the Montpellier Ressources Imagerie (MRI ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were resuspended in autoMACS buffer and labeled with anti-PSA-NCAM-APC (Miltenyi Biotec) for 10 min at 4°C in the dark ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Dead Cell Removal Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) was used to guarantee cell viability ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated with anti-APC MicroBeads (Miltenyi Biotec) for 15 min at 4°C in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated the samples for a total of 1h at RT with intermittent pulses of mechanical tissue disruption using the gentleMACSTM tissue homogenizer (Miltenyi Biotec). The resulting homogenate was washed twice using 10 ml of ice-cold 1% BSA/PBS and pelleted for 5min at 800g (4°C) ...
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bioRxiv - Developmental Biology 2023Quote: ... Three-dimensional images following tissue clearing were acquired with a light-sheet microscope UltraMicroscope II (Miltenyi Biotec).
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bioRxiv - Developmental Biology 2023Quote: ... endothelial cells (ECs) were isolated by incubation with CD31-coated magnetic microbeads (130-097-418, Miltenyi Biotec) and the positive selection was performed using a magnetic MACs separator ...
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bioRxiv - Developmental Biology 2023Quote: ... and CD45-positive cells (CD45 microbeads, 130-052-301, Miltenyi Biotec). Each depletion step consisted of incubating the cell suspension with magnetic beads (myelin removal or CD45 microbeads ...
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bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
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bioRxiv - Developmental Biology 2023Quote: ... Astrocytes were isolated from the remaining cell suspension using the anti-ACSA-2 microbead kit (130-097-678, Miltenyi Biotec). The cell suspension was incubated with FcR blocking reagents followed by anti-ACSA-2 microbeads and placed on a magnetic MACS separator for positive selection ...
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bioRxiv - Developmental Biology 2023Quote: ... Cerebral cortex was minced to small 2-3 mm pieces and dissociated using Neural Tissue Dissociation Kit P compounds (Miltenyi Biotec, 130-092-628) to obtain a cell suspension ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stained with human-EpCAM-APC (Miltenyi Biotec, 130-113-260) and CD276-PE (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... (Miltenyi Biotec, # REA293) was used as a background control ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution; Miltenyi Biotec), TGF-β1 (5 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were treated with a PE-conjugated anti-CD25 Ab (Miltenyi Biotec), PE-conjugated anti-CCR8 Ab (eBioscience) ...
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bioRxiv - Cancer Biology 2023Quote: CD4+ T cells were magnetically isolated from the spleens of healthy C57B6129SF1/J mice using a mouse CD4+ T cell isolation kit (Miltenyi Biotec). The CD4+ T cells were cultured for 3 days with or without KPC-derived PDAC cells in the presence of low dose anti-CD3/CD28 beads (x 40 dilution ...
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bioRxiv - Cancer Biology 2023Quote: Tumors from orthotopic PDAC mice were minced into 2-4 mm pieces and added with an enzyme mix from a tumor dissociation kit (Miltenyi Biotec, Bergisch Gladbach, Germany) in Roswell Park Memorial Institute medium 1640 (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... After removing dead cells with a Dead Cell Removal kit (Miltenyi Biotec), the cells were treated with a PE-conjugated anti-CD25 Ab (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2023Quote: Naïve CD4+ T cells and nTregs were isolated from the spleens of healthy C57B6129SF1/J mice by magnetic separation using a mouse naïve CD4+ T cell isolation kit (Miltenyi Biotec). The T cells were stimulated with anti-CD3/CD28 beads (x 25 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... PE-conjugated anti-mouse CD25 Ab (clone 7D4; Miltenyi Biotec), PE/Cyanine7-conjugated anti-mouse CD25 Ab (clone PC61 ...
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bioRxiv - Biophysics 2023Quote: ... buffer (2 mM EDTA and 0.5% fetal calf serum (FCS)) and microglia were isolated by positive selection using CD11b microbeads (Miltenyi Biotec, Gladbach, Germany), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The anti-mouse CD117-PE(Miltenyi Biotec, #REA791) antibody was used ...
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bioRxiv - Biophysics 2023Quote: ... 20 ng/mL epidermal growth factor (EGF; Miltenyi Biotec, Gaithersburg, MD), 0.5 mg/mL hydrocortisone (H0888 ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissues were dissociated using a gentle MACS Dissociator (Miltenyi Biotec) followed by a 40 min incubation at 37 °C ...