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Citations for Miltenyi Biotec :
2901 - 2950 of 3316 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... were co-transferred with CD3+ cell-depleted autologous PBMCs (2 × 106) isolated using CD3 Microbeads and the LD Column (Miltenyi Biotec) into 5- to 7-week-old female mice ...
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bioRxiv - Immunology 2021Quote: ... Untouched peripheral blood cDC1 and cDC2 (cDCs) and pDCs were obtained from PBMC after negative immunomagnetic separation with the Myeloid Dendritic Cell Isolation kit (Miltenyi Biotec) and the Plasmacytoid Dendritic Cell Isolation kit II (Miltenyi Biotec) ...
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bioRxiv - Bioengineering 2021Quote: ... The general surface marker composition for the sEVs from the three cell sources (HEK293, cbMSC, THP1) was assessed by a multiplex bead-based flow cytometry assay (MACSPlex Exosome Kit, human, Miltenyi Biotec) by using an optimized workflow as described previously.[27] In brief ...
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bioRxiv - Bioengineering 2021Quote: ... Peripheral neutrophils were isolated by negatively removing all contaminating cells using the MACSxpress Neutrophil Isolation Kit (Miltenyi Biotec, 130-104-434) and BD Pharm Lyse buffer (BD Biosciences ...
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bioRxiv - Bioengineering 2021Quote: ... We suspended microbead pellets in additional sorting buffer (500 μL for 108 cells) and then loaded the suspended microbeads to a LS column (Miltenyi Biotec). We washed the column that was attached to a magnetic MidiMACSTM separator from Miltenyi Biotec for retaining CD3 magnetic microbeads with the sorting buffer three times to remove CD3-negative cells and then transferred the beads bound with T cells to a 15 mL falcon tube ...
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bioRxiv - Neuroscience 2020Quote: ... were prepared from embryonic day E14.5 mice as described before [54] or from postnatal day 7-8 mouse brains using the MACS cell separation technology using the Neural Tissue Dissociation Kit and anti-O4 MicroBeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... The tumor-bearing hemispheres and corresponding hemispheres from naïve animals were dissociated enzymatically to obtain a single-cell suspension with a Neural Tissue Dissociation Kit with papain (Miltenyi Biotec) or 0.5 mg/mL DNase I (DN25 ...
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bioRxiv - Physiology 2021Quote: Bone marrow monocytes and neutrophils were isolated from C57BL/6J mice using MACS cell separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany) after incubation with either anti-Ly6G-PE (clone 1A8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pulse-treated cells and non-treated cells harboring a different fluorophore were then mixed in a 50:50 ratio confirmed by flow cytometry (MACSQuant; Miltenyi Biotec). Gates were established using non-fluorescent parental cell lines as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed and then depleted of lineage-positive cells by passing through a magnetic separation column (MACS LD Column, Miltenyi Biotec) loaded on a DynaMag-5 Magnet (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The dispersed islet cells were then resuspended in 150 ml of Nicoletti buffer containing 50 mg/ml propidium iodide (Miltenyi Biotec), 0.1% (wt/v ...
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bioRxiv - Cell Biology 2020Quote: Pre-conditioned media from FAP cell culture stimulated 3 days with PDGF-AA (20 ng/ml, human PDGF-AA, Cat nb. 130-108-983, Miltenyi Biotec) or control was collected and stored at −20°C ...
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bioRxiv - Biophysics 2021Quote: ... The pellet was solubilized for 30 minutes at 4°C and cell lysates were purified using the μMacs GFP isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 400 μL of 1x AnnexinV Binding buffer was added to each tube and cells were analyzed on a MACSQuant VYB (Miltenyi Biotec). A minimum of 10 000 cells were analyzed per condition ...
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bioRxiv - Cancer Biology 2022Quote: Fresh tumor tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using the Multi Tissue Human Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and fresh tissue was minced to 2-4 mm sized pieces in a 6-cm dish and subsequently digested to single cell suspension using Multi Tissue Mouse Tumor Dissociation Kit 1 (Miltenyi Biotec) and a gentleMACS OctoDissociator (Miltenyi Biotec ...
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bioRxiv - Physiology 2022Quote: ... The cell pellet was re-suspended in a sorting buffer and then passed through a magnetized LS separation column (Miltenyi Biotec). The iron-containing superparamagnetic cells/aggregates were eluted from the demagnetized column ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... Lung cells were centrifuged and resuspended with fresh media with 20 μL/mL of PeptTivator® SARS-CoV-2 Prot_S (Miltenyi Biotec) and 5 μg/mL Brefeldin A (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Scotland) followed by CD14+ selection via magnetic cell separation using MACS CD14 MicroBeads and separation columns (Miltenyi Biotec, Bergisch Gladbach, Germany), as previously described (31) ...
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bioRxiv - Immunology 2022Quote: ... we assessed the capacity of NK cells to perform ADCC after being stimulated with IFN-α or IL-15 (Miltenyi Biotec). In such cases ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells were resuspended with 100 µl of Inside Perm Buffer (Inside Stain Kit, Miltenyi Biotec). Eight µl of anti-GFAP and 2.5 µl of anti-Iba1 were added to the cell suspensions and mixed by gently pipetting ...
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bioRxiv - Cancer Biology 2022Quote: ... the supernatant was removed and the cells resuspended with 400 µl of Running Buffer composed of MACS BSA stock solution (Miltenyi Biotec) 1:20 with autoMACS Rinsing Solution (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2022Quote: ... Biopsies were manually homogenized using sharp dissection between digest steps and by blunt homogenization through a 100 μm cell strainer (Miltenyi Biotec). Centrifugation steps were performed at 300 x g for 5 min at 4°C.
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bioRxiv - Immunology 2020Quote: ... the SILP and the CLP single cell suspensions were obtained using the Lamina Propria Dissociation Kit (Miltenyi Biotec SAS, Paris, France) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... depleted of CD8+ T cells using a negative selection step (CD8 MicroBead Kit; LD columns, QuadMACS Separator, all from Miltenyi Biotec) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... The isolation of lineage negative (Lin-) HSPCs or neutrophils were performed using Lineage Cell depletion kit or Neutrophil Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Ly6G+ cells were isolated by Neutrophil isolation Kit mouse and F4/80+ cells by Anti-F4/80 Microbeads Ultrapure mouse kit followed the manufactory instructions (MACS, Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... the corresponding mAbs were added for 30 minute and cells were washed with BD Perm/Wash Buffer 1X before acquisition in the MACSQuant Analyzer 10 flow cytometer (Miltenyi Biotec). The percentage of positive cells for CD107a ...
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bioRxiv - Neuroscience 2022Quote: ... the contents of the gentleMACS™ C Tube were strained through a 70 μm cell strainer (Miltenyi Biotec, # 130-110-916) placed on top of a 15 mL conical centrifuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were passed through a 45 μm Nitex mesh to remove undissociated cell clumps and after addition of myelin removal beads (Miltenyi Biotec), passed through a MACS column (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2022Quote: ... On day five ECs were dissociated with StemPro Accutase (diluted 1:6 in D-PBS) and separated via Magnetic-activated cell sorting (MACS, Miltenyi Biotec) using CD34 MicroBeads ...
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bioRxiv - Immunology 2022Quote: ... Fluorescence spillover compensation for cell sorting was performed using lymphocytes stained with CD4 (OKT4) in the respective colors and with compensation beads (Miltenyi Biotec) for flow cytometry ...
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bioRxiv - Cell Biology 2022Quote: ... incubated for 15 min at room temperature and applied onto the dead cell removal column (cat # 130-042-401, Miltenyi Biotec), which was attached to the MACS separator ...
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bioRxiv - Immunology 2022Quote: ... Cells were counted and CD11b+ cells were selected by MACS column purification using the CD11b MicroBead kit and LS columns (Miltenyi Biotec). CD11b+ cells were stained with Fc block α-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Remaining cells were then magnetically labeled with HLA-G – PE antibody and anti-PE microbeads and enriched for HLA-G+ EVTs by magnetic-activated cell sorting using an OctoMACS separator and MS columns (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2022Quote: ... MDSC enriched bone marrow cells were collected from culture as described above and subjected to M-MDSC magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: CD8+ T cells were isolated from the spleens of transplanted PyMT tumor-bearing mice with a CD8a+ T Cell Isolation Kit (#130-104-075, Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cells using dissociation kits according to the manufacturer’s protocol (preserving all surface epitopes, Miltenyi Biotec #130-095-927), then stained with APC-conjugated antibodies against CD45 (eBioscience #17-0451-83) ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were purified using a CD11b+ isolation kit per the manufacturer’s recommended protocol (Miltenyi Biotec, Auburn, CA; cat# 130-091-100). Prior to setting the culture ...
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bioRxiv - Neuroscience 2020Quote: ... The cell pellet was washed with DMEM/10% FBS followed by CD11b positive selection (Miltenyi Biotec, Cat. No. 130–093-636) using mini-MACS (Miltenyi Biotec ...
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bioRxiv - Immunology 2020Quote: Total CD4+ T cells from baseline leukapheresis were isolated from cryopreserved PBMCs by manual magnetic labeling and negative selection using the CD4+ T Cell Isolation Kit (Miltenyi Biotec).
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bioRxiv - Genomics 2020Quote: ... The cell suspension is pipetted through Falcon tubes with cell-strainer caps and then analyzed by MACSQuant Analyzer 10 Flow Cytometer (Miltenyi Biotec) for 10,000 events.
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bioRxiv - Cancer Biology 2021Quote: ... and from CD45− cells isolated from WT and CCN4-KO tumors by negative selection using a mouse CD45 MicroBeads kit (Miltenyi Biotec). Conditioned media were obtained by culturing 1 ×104 CD45− cells/well for 36 h in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... HTLV-1-infected cells were sorted as CD4+CADM1+ T-cells using FITC-labeled anti-CADM1 mAb (3E1, MBL) and anti-FITC MicroBeads (Miltenyi Biotec). The CADM1- fractions were used as normal CD4+ T-cells ...
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bioRxiv - Immunology 2020Quote: ... T cells were isolated from PBMCs by three sequential rounds of magnetic separation using CD3 Microbeads (Miltenyi Biotec, 130-050-101), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... CD34+ human hematopoietic progenitor cells were isolated from human fetal liver tissue (obtained from Advanced Bioscience Resources) using the CD34 MicroBead Kit (Miltenyi Biotec) following the protocol provided by the manufacturer ...
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bioRxiv - Immunology 2021Quote: CD8+ T cells were isolated from splenocytes of OT-I mice on C57BL/6 background using anti-CD8a microbeads (Miltenyi Biotec), and then fluorescently labeled with carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Immunology 2021Quote: ... WKy CD4+CD25high cells were isolated by magnetic and fluorescence-activated cell sorting and expanded with anti-CD3 and anti-CD28 coated beads (Miltenyi Biotec) and 1000 units/mL recombinant human IL-2 (rhIL-2 ...
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bioRxiv - Immunology 2021Quote: ... Four days later fresh DC culture medium was added and on day 8-9 of differentiation suspension and loosely adherent cells were harvested with gentle pipetting and HLA-DR+ cells were isolated by immunomagnetic bead selection (MACS) with HLA-DR microbeads (Miltenyi Biotec) following the manufacture’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... whole bone marrow (WBM) cells isolated by crushing femur and tibia from each mouse were incubated with 1x RBC Lysis Solution (Miltenyi Biotec) to eliminate red blood cells ...