Labshake search
Citations for Miltenyi Biotec :
2751 - 2800 of 3316 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... then astrocytes were isolated using the anti-ACSA-2 magnetic cell sorting kit and protocol (catalog #130097678, Miltenyi Biotec). RNA was purified and concentrated with proteinase K cell digestion ...
-
bioRxiv - Developmental Biology 2024Quote: ... The cell suspension was applied onto the MACS Column placed in a MACS Separator (Miltenyi Biotec, Bergisch Gladbach, Germany). We first collected the flow-through containing unlabeled cells ...
-
bioRxiv - Systems Biology 2024Quote: ... Splenic CD8+ T cells were isolated from mice (ZsGreen lox stop lox) by positive CD8a immunomagnetic selection (Miltenyi Biotec). Cells were labeled with antibodies against CD44 (IM7) ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells in the MNC-UCB fraction were isolated using CD34 MicroBead Kit UltraPure (130-100-453, Miltenyi Biotec) via LS Columns and MidiMACS Separator (Miltenyi Biotec) ...
-
bioRxiv - Cancer Biology 2024Quote: ... CD4+ T cells were then isolated from the spleen and lymph nodes of BALB/c mice (DO11.10) using a CD4+ T cell isolation kit according to the manufacturer’s instructions (Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were then washed with wash buffer and passed through an activated MS column (130-042-201, Miltenyi Biotec). The column was washed three times ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with wash buffer and then incubated with CD45 human Microbeads (130-045-801, Miltenyi Biotec) for 20 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were washed twice with PBS 2% FCS before analysis by flow cytometry using a MACSQuant cytometer (Miltenyi Biotec, Germany) and analysis was done with the software FlowJo (vers 7.6).
-
bioRxiv - Immunology 2021Quote: ... Total T cells from spleen of Fam13a KO mice and WT littermates were isolated with CD90.2 microbeads (130-121-278, Miltenyi Biotec). The cells were then stained with the antibody mix containing LIVE/DEAD® Fixable Near-IR (dilution 1:500) ...
-
bioRxiv - Cell Biology 2020Quote: Lineage negative and CD31 positive BMCs were isolated using first immunomagnetic Lineage Cell Depletion Kit (130-092-211, Miltenyi Biotec) followed by positive selection with CD31 MicroBead Kit (130-091-935 ...
-
bioRxiv - Cell Biology 2020Quote: ... Naïve CD4+ T cells were isolated from the lymph nodes and spleen of C57BL/6 mice by MidiMACS (Miltenyi Biotec). Cells were activated by plate-bound anti-CD3 (5 μg mL−1 ...
-
bioRxiv - Developmental Biology 2021Quote: ... and seeded at a density of 19,000 cells/cm2 on Matrigel in mTeSR1 supplemented with 10 μM Rho-associated protein kinase inhibitor Y-27632 (Miltenyi Biotec). Upon reaching 95% confluence ...
-
bioRxiv - Immunology 2021Quote: Naive CD4+ or CD8+ T cells were positively selected from single-cell suspensions prepared from spleen and lymph node by mechanical disruption using streptavidin MicroBeads according to manufacturer’s instructions (Miltenyi Biotec). Purified cells were activated in twelve-well plates coated with 0.1-10 μg/mL αCD3 (145–2C11 ...
-
bioRxiv - Developmental Biology 2021Quote: ... the tip epithelial cells were isolated by EPCAM+ magnetic-activated cell sorting (MACS) beads according to the manufacturer’s instruction (CD326 MicroBeads, human, Miltenyi Biotec) from the distal lung tissues ...
-
bioRxiv - Neuroscience 2021Quote: ... ACSA-2 labelled cells were flushed from the MS column with pre-warmed AstroMACS medium (Miltenyi Biotec, 130-117-031) supplemented 50 U/ml penicillin/streptomycin (Sigma ...
-
bioRxiv - Neuroscience 2021Quote: ... Red blood cells and debris were removed and astrocytes magnetically labelled using ACSA-2 microbeads (Miltenyi Biotec, 130-097-678) according to manufacturer’s protocol ...
-
Optimization of AAV6 transduction enhances site-specific genome editing of primary human lymphocytesbioRxiv - Molecular Biology 2021Quote: ... Human CD4+ T cells were isolated by positive magnetic selection using human CD4 MicroBeads kit (Miltenyi Biotec, Bergisch Gladbach, Germany) per manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: CD4+ cells were isolated from C7 Tg mouse spleens using mouse CD4 (L3T4) MicroBeads from Miltenyi Biotec (130-117-043) according to the manufacturer’s protocol and cultured in T cell media at 2 x 106 cells/mL with 1 μM ESAT-6 peptide pool (NIH BEI Resources Repository catalog no ...
-
bioRxiv - Immunology 2020Quote: ... T cells were purified with either anti-CD8 or anti-CD4 magnetic beads using MACS columns (Miltenyi Biotec, Auburn, CA) prior to cell surface staining ...
-
bioRxiv - Genomics 2020Quote: ... CD34 positive cells were then bead enriched using the CD34 MicroBead Kit UltraPure (Miltenyi Biotec, catalog number 130-100-453) following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
-
bioRxiv - Immunology 2021Quote: ... Lungs from infected mice were digested using the Miltenyi lung cell isolation buffer and dissociated via GentleMACS (Miltenyi Biotec, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: ... Single cell suspensions were generated using the Neural Tissue Dissociation Kit – Postnatal Neurons (Miltenyi Biotec. Cat no. 130-094-802), following manufacturer’s instructions for manual dissociation ...
-
bioRxiv - Neuroscience 2020Quote: ... Cell suspension was obtained by automated dissociation using the Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Cat#130-092-628) and the gentleMACS™ Dissociator (Miltenyi Biotec ...
-
bioRxiv - Bioengineering 2021Quote: ... and the cell pellet was gently re-suspended in Red Blood Cell Lysis Solution (freshly prepared according to manufacturer’s instruction; 130-094-183; Miltenyi Biotec B.V ...
-
bioRxiv - Microbiology 2020Quote: ... CD4+ and CD8+ T cells were isolated by MACS from monocyte-depleted PBMC using CD4 and CD8 microbeads (Miltenyi Biotec). These primary cells were cultured in X-vivo 15 (Lonza ...
-
bioRxiv - Microbiology 2020Quote: ... T cells from the lungs of naïve or infected mice (day 8 pi) were enriched using the Pan T cell Isolation Kit II (Miltenyi Biotec) and co-cultured with infected BMDC at a ratio of 10:1 for 2-3 hours followed by the addition of Brefeldin A (5µg/ml ...
-
bioRxiv - Cell Biology 2021Quote: ... at 37°C for 1 hr and passed through a 70 μm and a 30 μm cell strainer (Miltenyi Biotec). CD45+ cells were isolated using magnetic beads (Miltenyi Biotec 130-052-301 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Single mouse tumor cell suspensions were obtained using a mouse Tumor Disassociation Kit from Miltenyi Biotec (Cat# 130-096-730). After leukocytes extraction using density gradient medium ...
-
bioRxiv - Molecular Biology 2022Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
-
bioRxiv - Immunology 2022Quote: ... total CD4+ T cells were directly isolated from buffy coats using the StraightFrom Buffy Coat CD4 MicroBead kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2022Quote: Primary NK cells were isolated and enriched the day of the experiment from the spleens of 8-12 week old female C57BL/6 mice using the NK Cell Isolation Kit II according to manufacturer’s instructions (Miltenyi Biotec). 50,000 NK cells were then seeded in the top chamber of a transwell insert (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec, 130097678). RNA was purified and concentrated with proteinase K cell digestion ...
-
bioRxiv - Immunology 2022Quote: ... PMEL T cells were isolated from the splenocytes of the Thy1.1+ PMEL TCR transgenic mice by CD8a+ negative selection (Miltenyi Biotec). Each irradiated Krt14-Kitl* mouse received 1×106 cells of PMEL plus 1.8×106 cells of the peptide-pulsed MHC-II+ ...
-
bioRxiv - Immunology 2022Quote: Neutrophils were purified from freshly isolated mouse bone marrow cell suspensions using an autoMACS pro-selector cell separator with a MACS neutrophil isolation kit for negative selection in accordance with the manufacturer’s instructions (Miltenyi Biotec). Neutrophils were aged overnight in serum-free medium at 3 × 106 cells/ml at 37 °C and 5 % CO2 ...
-
bioRxiv - Immunology 2022Quote: ... Next the cells were washed and incubated for 20 min at 4°C with 100μl anti-FITC MACS magnetic beads (Miltenyi Biotec) per 108 cells ...
-
bioRxiv - Cell Biology 2022Quote: ... Fresh tumor tissue from NSCLC patients were enzymatically digested and single-cell suspensions were stained with CD14 microbeads (Miltenyi Biotec) and positively selected using AutoMACS ProSeparator (Miltenyi Biotec) ...
-
bioRxiv - Cell Biology 2021Quote: ... Human CD34+ cells were then enriched in the sample by immunomagnetic beads using an AutoMACSpro (Miltenyi Biotec, Bergisch Gladbach, Germany). After collection ...
-
bioRxiv - Neuroscience 2020Quote: ... The brain was dissociated into single cell suspension using the Neural Tissue Dissociation Kit P (Miltenyi Biotec, 130-092-628) according to the manufacturers protocol ...
-
bioRxiv - Immunology 2020Quote: Splenic B cells of both genotypes were CD43-depleted using magnetic beads (130-049-801, Miltenyi Biotec, Bergisch Gladbach Germany) and taken in culture in the presence of 1μg/ml anti-CD40 antibody (102901 ...
-
bioRxiv - Immunology 2020Quote: ... Tregs were purified after enrichment of CD25+ cells using biotinylated anti-CD25 mAb (7D4) and anti-biotin microbeads (Miltenyi Biotec), followed by CD4 staining (RM4.5 ...
-
bioRxiv - Microbiology 2021Quote: ... CV38-142 IgG used in the SPR assay was produced in CHO cells and was kindly provided by Miltenyi Biotec, Bergisch Gladbach ...
-
bioRxiv - Cell Biology 2020Quote: ... On day 15 of cardiac differentiation fixed cells were labeled for flow cytometry using alpha-actinin (Miltenyi Biotec, Cat.#130106937) and corresponding isotype control ...
-
bioRxiv - Microbiology 2020Quote: ... For the isolation of pDCs from PBMCs the Plasmacytoid Dendritic Cell Isolation Kit II from Miltenyi Biotec (# 130-097-415) was used ...
-
bioRxiv - Neuroscience 2021Quote: ... Positive selection of NeuN+ cells was then performed according to the standard MACS MS Columns protocol available from Miltenyi Biotec. After the first round of magnetic separation ...
-
bioRxiv - Neuroscience 2022Quote: ... Motoneurons were isolated from the spinal cord of Smn2B/+ and Smn2B/- embryos using 5.2% iodixanol density gradient centrifugation combined with p75-based magnetic cell isolation (Miltenyi Biotec) as we previously described (Soulard et al. ...
-
bioRxiv - Developmental Biology 2022Quote: A dissociated single-cell suspension was passed through 30 µm nylon mesh (Pre-Separation Filters; Miltenyi Biotec #130-041-407) to remove cell clumps that could clog the column ...
-
bioRxiv - Developmental Biology 2022Quote: ... Each purified hepatocyte pellet was resuspended in 5ml of red blood cell (RBC) lysis solution (Miltenyi Biotec 130-094-183) and incubated at room temperature for 10 mins ...
-
bioRxiv - Immunology 2022Quote: ... CD4 T cells from HLA-DRb1*04-positive donors were magnetically purified using CD4 microbeads according to the supplier’s instructions (Miltenyi Biotec), while CD3+/CD4+/CD8αlow/CCR6+/CXCR6+ F ...
-
bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing NPCs was counted and Kupffer cells (KC) were removed using anti-F4/80 Ultrapure microbeads (Miltenyi Biotec) following manufacturer’s guidelines ...