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Citations for Miltenyi Biotec :
2701 - 2750 of 3316 citations for Killer Cell Immunoglobulin Like Receptor 2DL3 KIR2DL3 Antibody PE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cell concentrates were enriched in CD34+ HSPCs using a CliniMACS Plus instrument (Miltenyi Biotec, Gaithersburg, MD, USA) and cryopreserved prior to use ...
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bioRxiv - Neuroscience 2023Quote: ... and processed to create a single-cell suspension using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) supplemented with Actinomycin D (#A1410 ...
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bioRxiv - Cancer Biology 2023Quote: Primary bone marrow-derived white blood cells were sorted for CD34 positivity using a CD34 MicroBead Kit (Miltenyi Biotec) and cultured for 10 days as detailed above (primary cultures ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Neuroscience 2023Quote: ... Analyses of the labeled cells were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). The proportion of eGFP+ cells that co-labelled with CD11b+CD45+ was assessed and compared between groups using MACSQuantify v2.13.0 software.
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cells were collected and incubated with CD45 magnetic microbeads (130-045-801; Miltenyi Biotec, Bergisch Gladbach, Germany) for 30 minutes at 4℃ in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Analysis for plasma- and cell line-derived EV samples was performed using the MACSQuant® Analyzer 16 (Miltenyi Biotec) and the corresponding software MACSQuantify™ (Version 2.13.3) ...
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bioRxiv - Immunology 2023Quote: ... Further purification of immune cells was done by magnetic enrichment of the CD45+ fraction (130-052-301, Miltenyi Biotec). All cell suspensions were kept in PEB buffer (0.5% BSA and 2 mM EDTA in PBS).
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bioRxiv - Neuroscience 2023Quote: Cells were isolated from the adult brain using the Adult Brain Dissociation kit (Miltenyi Biotec, cat # 130-107-677) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
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bioRxiv - Cancer Biology 2023Quote: ... after which CD34+ stem cells were isolated by MACS sorting using CD34 MACS microbeads (Miltenyi Biotec, Leiden, The Netherlands) following manufacturers recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated for 10 min with 25 µl of FcR Blocking Reagent (Miltenyi Biotec 130-059-901) (pre-diluted 1:50 with FACS buffer (PBS ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes of P2-TCRα retrogenic mice were enriched for CD4+ cells using CD4 microbeads (Miltenyi Biotec, 130-117-043). Teffs (≤ 140,000 cells per mouse ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Cancer Biology 2023Quote: ... CD138+ cells were isolated from bone marrow aspirate samples using Human CD138 MicroBeads (Miltenyi Biotec, Cat # 130-051-301). Selected cells were either viably cryopreserved in 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor samples were dissociated into single cells using GentleMACS tissue dissociator and its kit (Miltenyi Biotec, Gladbach, Germany). The tumor samples were cut into small pieces ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspension was used for the isolation of neurons using the Adult Neuronal Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads (Miltenyi Biotec, Ref#130-090-855) with auto MACS pro (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: Astrocytes were isolated by magnetic assisted cell sorting (MACS) approach using the anti-ACSA2 microbeads (Miltenyi Biotec Inc., Germany). Briefly ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspensions of the clinic tumor tissues mentioned above were prepared by Tumor Dissociation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: SIY-reactive CD8+ T cells were isolated from the spleen and lymph nodes from TCR-transgenic 2C CD45.1+ or 2C CD45.2+ Rag2-/- mice using the CD8a+ T cell isolation kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: We dissociated cells with Trypsin-EDTA or with the Embryoid Body dissociation kit (cod. 130-096-348 Miltenyi Biotec). Dissociated cells (1×106 cells/100 μl ...
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bioRxiv - Immunology 2023Quote: ... Human peripheral blood mononuclear cells purchased from STEMCELL (70025) was stimulated by OKT-3 (Miltenyi Biotec, 130-093-387) and CD28 (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: We prepared B cell tetramers of biotinylated S2hlx-Ex19 or FP-10 by mixing with Streptavidin-VB515 (Miltenyi Biotec) and Streptavidin-AlexaFluor 647 (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: Isolation of endothelial cells and fibroblasts from adult mouse hearts was performed with MACS-magnetic beads from Miltenyi Biotec based on the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... Tissue pieces were dissociated into single cell suspensions with the human tumor dissociation kit following manufacturer instructions (Miltenyi Biotec). Digestion was stopped with 20% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... in cell culture media supplemented with M-CSF (25 µg/mL, mouse M-CSF #130-101-704, Miltenyi Biotec) and antibiotics ...
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bioRxiv - Cancer Biology 2024Quote: ... and was dissociated into a single cell suspension using the Human Tumor Dissociation kit (Miltenyi Biotec, #130-095-929) on a gentleMACS™ Octo Dissociator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... These cells were mixed with CD4+ beads and separated via MACS LS Columns (both Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: The isolated NK cells were maintained in the NK MACS Basal medium supplemented with NK MACS supplement (Miltenyi Biotec), 5% human AB serum (Sigma) ...
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bioRxiv - Immunology 2024Quote: CD4+ T cells were pre-enriched from spleens of CD45.1 or CD45.1/2 OT-II mice by negative selection using the CD4+ T-cell isolation kit (Miltenyi Biotec). Live (SytoxBlue−)CD25− CD44loCD62L+TCRβ+vα2+ naïve OT-II T cells were FACS-isolated ...
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bioRxiv - Immunology 2024Quote: ... Purification of primary pDC was performed using the Plasmacytoid Dendritic Cell Isolation Kit II (#130-097-415 Miltenyi Biotec), reaching >93% of purity ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were purified by incubation with anti-CD4 microbeads and passing through a magnetic column (Miltenyi Biotec). The purity of cells was higher than 95% as determined by flow cytometry ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were positively selected from the tumor digest with Miltenyi LS columns (Miltenyi Biotec, cat. 130-042-401) and MidiMACS separator (Miltenyi Biotec ...
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bioRxiv - Microbiology 2024Quote: ... CD3+/CD4+ T cells were purified by positive selection using magnetic separation with a CD4+ isolation kit (Miltenyi Biotec) from PBMCs as per the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... then astrocytes were isolated using the anti-ACSA-2 magnetic cell sorting kit and protocol (catalog #130097678, Miltenyi Biotec). RNA was purified and concentrated with proteinase K cell digestion ...
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bioRxiv - Immunology 2024Quote: CD8 T cells were isolated from spleens of OT-1 mice using the CD8 mouse isolation kit (Miltenyi Biotec) according to the instructions of the manufacturer ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Naïve B cells (CD43-B220+CD3-) were obtained by negative selection (mouse CD43 Ly-48 MicroBeads; MACS Miltenyi Biotec) from spleens of WT and Lrba-/- mice ...
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bioRxiv - Cancer Biology 2024Quote: ... CD34+ cells in the MNC-UCB fraction were isolated using CD34 MicroBead Kit UltraPure (130-100-453, Miltenyi Biotec) via LS Columns and MidiMACS Separator (Miltenyi Biotec) ...