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Citations for Miltenyi Biotec :
2751 - 2800 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell surface-stained cells were fixed for 30 min at room temperature with Foxp3 fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5523-00 or Miltenyi Biotec, 130-093-142). After a washing step with FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... SiglecF-positive cells were enriched using anti-PE MultiSort Kit (Miltenyi Biotec). 1x105 flow through and AM enriched fractions were co-cultured with 1x105 hCD1Tg BMDCs and 3x105 DN1 T cells for 48 hours and cells were stained for the expression of activation markers.
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bioRxiv - Developmental Biology 2023Quote: ... in autoMACS® Running Buffer (Miltenyi Biotec, Germany) for 30 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:50 in MACS® BSA Stock Solution and autoMACS® Rinsing Solution (1:20, Miltenyi Biotec, Germany) for 75 min at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... diluted 1:50 in MACS® BSA Stock Solution and autoMACS® Rinsing Solution (1:20, Miltenyi Biotec, Germany) for 1-2h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... then incubated with anti-Mouse IgG micro-bead (1:10 in autoMACS Running Buffer, Miltenyi Biotec, Germany) for 30 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: Cell separation was done using MACS® MicroBeads cell separation system (Miltenyi Biotec, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Microglia were isolated according to manufacturer’s instructions using Myelin Removal Beads II (Miltenyi Biotec). Microglia used for qRT-PCR analysis were further sorted using CD11b MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: B cells were purified from UCBMC using MACS CD20+ microbeads (Miltenyi Biotec, Waltham, MA). 50-100,000 B cells were plated per well and stimulated using a TLR agonist cocktail containing LPS (100 μg/μL) ...
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bioRxiv - Cancer Biology 2023Quote: Primary bone marrow-derived white blood cells were sorted for CD34 positivity using a CD34 MicroBead Kit (Miltenyi Biotec) and cultured for 10 days as detailed above (primary cultures ...
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bioRxiv - Bioengineering 2023Quote: ... cells were filtered through a 30 μm mesh-filter (Miltenyi Biotec), centrifuged ...
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bioRxiv - Bioengineering 2023Quote: ... with the exception that dissociated β-like cells were directly incubated with anti-CD19 MicroBeads (Miltenyi Biotec) instead of a primary antibody.
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bioRxiv - Bioengineering 2023Quote: ... [4] (Miltenyi Biotec), and the lineage-negative NSG HSPCs were collected after MACS depletion of the lineage-positive marrow cells ...
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bioRxiv - Immunology 2023Quote: Monocytes were isolated from the bone marrow of Apoe-/-LysMCre and Apoe-/-LysMCreCnr1flox/flox mice by magnetic separation monocyte Isolation Kit (130-100-629, Miltenyi Biotec). The purified monocytes were labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE ...
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bioRxiv - Cancer Biology 2023Quote: T cells were isolated using Pan T magnetic isolation beads (Miltenyi Biotec) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and placed into gentleMACS C-tubes (#130-093-237, Miltenyi Biotec), and processed to create a single-cell suspension using the Adult Brain Dissociation Kit (#130-107-677 ...
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bioRxiv - Neuroscience 2023Quote: ... the sample and collection tubes were placed in a cold Chill5 tube rack (#130-092-951, Miltenyi Biotec), collecting both positive and negative fractions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376, Miltenyi Biotec) and filtered through a 35 µm filter (#352235 ...
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bioRxiv - Neuroscience 2023Quote: ... and homogenized on the gentleMACS Octo Dissociator System (#130-095-937, Miltenyi Biotec), as previously described (12) ...
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bioRxiv - Neuroscience 2023Quote: ... the second aliquot containing 1 µL of CD11b-APC antibody (#130-113-793, Miltenyi Biotec); the third aliquot containing 1 µL of CD45-VioBlue antibody (#130-110-802 ...
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bioRxiv - Neuroscience 2023Quote: ... and processed to create a single-cell suspension using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) supplemented with Actinomycin D (#A1410 ...
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bioRxiv - Neuroscience 2023Quote: ... Following debris removal (#130-109-398, Miltenyi Biotec), cells were resuspended in 1 mL 0.5% BSA in D-PBS (#130-091-376 ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Developmental Biology 2023Quote: ... filtered through 30 uM and 20 uM filters (Miltenyi Biotec) and cleaned of debris using a Percoll (Sigma ...
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bioRxiv - Immunology 2023Quote: ... or MACSQuant (Miltenyi Biotec, Bergisch Gladbach, Germany) and data analyzed using FlowJo software V.9.0 or V.10.0 (Treestar ...
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bioRxiv - Immunology 2023Quote: ... using CD40L-multimere-kit and IL-4 (Miltenyi Biotec) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and the AutoMACS Pro magnetic cell separation system (Miltenyi Biotec). Samples were stained with DRAQ7 (BioStatus ...
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bioRxiv - Immunology 2023Quote: ... The numbers of viable cells were determined using propidium iodide and a MACSQuant (Miltenyi Biotec). Before cell sorting ...
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bioRxiv - Cancer Biology 2023Quote: ... Human tumor cells were isolated from primary human omental metastases with human tumor cell isolation kit (Miltenyi Biotec, Cat# 130-108-339) using MACS® cell separation technology following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cell concentrates were enriched in CD34+ HSPCs using a CliniMACS Plus instrument (Miltenyi Biotec, Gaithersburg, MD, USA) and cryopreserved prior to use ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue was disrupted by running program m_lung_01 on the gentleMACS dissociator (Miltenyi Biotec) followed by 30 min incubation at 37°C with continuous shaking ...
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bioRxiv - Genetics 2023Quote: ... supernatant was taken and then filtered using a 30 μm cell strainer (Miltenyi Biotec, 130-041-407) into a nuclease free collection tube ...
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bioRxiv - Genomics 2023Quote: ... the tissue was chopped into small pieces with scissors and then transferred along with the lysing buffer into a gentleMACS C tube (Miltenyi Biotec, Cat. 130-096-334). An additional 1 ml of lysing buffer was added into the C tube to make a final volume of 2 ml ...
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bioRxiv - Genomics 2023Quote: ... 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec, 130-091-222]).
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Immunology 2023Quote: ... B cells were obtained by negative selection using anti-CD43 beads (Miltenyi Biotec), as per the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... followed by anti-biotin beads (Miltenyi Biotec), as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2023Quote: ... on the gentleMACS Dissociator (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Excised tumours were harvested and dissociated with Tumour Dissociation Kit (130-096-730, Miltenyi Biotec B.V ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stained with antibodies specific for PD-L1 (Miltenyi Biotec) and ErbB2 (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... and tissue was dissociated on the gentleMACS Dissociator (Miltenyi Biotec) as previously described22 ...
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bioRxiv - Neuroscience 2023Quote: ... in mTeSR1 and fed daily with medium plus 50 ng/ml BMP4 (Miltenyi Biotec), 50 ng/ml VEGF (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ng/ml VEGF (Miltenyi Biotec), and 20 ng/ml SCF (R&D Systems) ...
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bioRxiv - Cancer Biology 2023Quote: ... on a MACS MultiStand (Miltenyi Biotec cat # 130-042-303). The cells collected in the flow-through and subsequent rinses were the unlabeled EpCAM-cells ...