Labshake search
Citations for Miltenyi Biotec :
2551 - 2600 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Flow cytometry with anti-cytokeratin-PE (Miltenyi Biotec, clone ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with antibodies against viability fixable dye (Miltenyi Biotec, #130-109-812), CD3 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: ... using CD4 microbeads (Miltenyi Biotec, Germany) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were purified by positive selection using Miltenyi magnetic beads (Miltenyi Biotec, Gladbach, Germany). These cells were stimulated with anti-CD3/CD28 magnetic Dynabeads (1 bead per cell ...
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bioRxiv - Cell Biology 2023Quote: ... The magnetically labelled samples were applied to the column which was placed in a mMACS Separator (Miltenyi Biotec). The column was washed 4x with Isolation Buffer and then placed in 1.5mL tubes ...
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bioRxiv - Cell Biology 2023Quote: ... The sample was eluted by adding 100µL RNA lysis buffer (Miltenyi Biotec) to the column and flushed out by firmly pushing the plunger into the column ...
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bioRxiv - Cell Biology 2023Quote: ... CD63 or CD81 MicroBeads (Miltenyi Biotec) overnight at 4°C in a tube rotator in the absence of light ...
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bioRxiv - Biophysics 2023Quote: ... Peripheral mononuclear cells were collected by density gradient centrifugation and enriched using a CD8+ T cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumor pieces were dissociated using a modified protocol based on Miltenyi (mouse) Tumor Dissociation Kit (Miltenyi Biotec, 130-096-730). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... total leukocytes were depleted from monocytes with CD14 MicroBeads UltraPure (Miltenyi Biotec) according to manufacturer’s instruction and treated in autologous media.
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells (CD4+CD44-CD62L+) were isolated from mouse spleen by negative selection using the Naive CD4+ T Cell Isolation Kit (Miltenyi Biotec, 130-104-453). Purified naïve T cells were activated with plate-bound anti-CD3ε (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... Primary mouse CD4+ T cells were cultured in complete TexMACS Medium (Miltenyi Biotec, #130-097-196) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: Previously cryopreserved PBMCs were thawed and CD8+ T cells were enriched by negative selection using a nonhuman primate CD8+ T cell isolation kit (Miltenyi Biotec). RNA was isolated from the enriched CD8+ T cells by TRIzol/phenol chloroform extraction with DNase treatment ...
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bioRxiv - Immunology 2023Quote: ... and CD38 (20 µl per 107 cells, from human CD38 MicroBead kit, Miltenyi Biotec) followed by depletion using magnetic anti-biotin beads (30 µl per 107 cells ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... and mitochondria were purified with the Human Mitochondria Isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Murine neutrophils were isolated from bone marrow of 7–12-week-old mice using an Anti-Ly-6G MicroBeads kit (Miltenyi Biotec; Cat. No. 130-120-337) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and anti-IgM-PE (clone PJ2-22H3, all purchased from Miltenyi Biotec, Bergisch Gladbach, Germany). Samples were measured using a FACSLyric flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... A MACS Quant analyzer (Miltenyi Biotec) and Flowjo (Tomy Digital Biology ...
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bioRxiv - Molecular Biology 2023Quote: ... 5.7 mg of beads from MACSflex™ MicroBead Starting Kit (Miltenyi Biotec) was reconstituted in 257 μl of Reconstitution Buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Human (Miltenyi Biotec), according to the manufacturer’s instructions with minor modifications ...
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bioRxiv - Microbiology 2023Quote: ... High-parasitemia schizont cultures were passed through MACS LD magnet columns (Miltenyi Biotec) and schizonts were collected ...
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bioRxiv - Microbiology 2023Quote: ... Single-cell suspensions of CD4+ or CD8+ T-cells were prepared from spleens by negative selection using magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Naive 6-week-old IFN-αβR-/- mice (n = 4-9 ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were harvested and the transfection efficiency as well as expression of the NSD2 constructs in HEK293 cells were evaluated based on the mVenus reporter by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and western blot using an anti GFP antibody (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... MLL-AF9 leukaemic blasts were generated by magnetic bead selection (Miltenyi Biotec) of c- KIT+ cells from whole mouse bone marrow and subsequent retroviral transduction with an MSCV-FLAGx3-MLL-AF9 construct ...
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bioRxiv - Microbiology 2023Quote: ... and 1:50 FcR Blocking Reagent (Miltenyi Biotec)) ...
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bioRxiv - Microbiology 2023Quote: ... 100 U/ml IL-2 (Miltenyi Biotec, Cat. No. 130-097-745) and 10 µM raltegravir (Selleck Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... lungs were harvested in 2 ml of Trizol and homogenized using a gentleMACS Octo dissociator (Miltenyi Biotec, Inc.). Total RNA was purified with trizol/chloroform ...
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bioRxiv - Microbiology 2023Quote: ... were gained from EDTA blood by density separation (Lymphocyte Separation Medium (AnproTech)) and CD8+ T-cells were isolated with the human CD8+ T-cell isolation kit (Miltenyi Biotec) and a MidiMACS LS-column according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... APC-coupled anti-CD56 (Miltenyi Biotec (130-113-305, RRID AB_2726084)) ...
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bioRxiv - Molecular Biology 2023Quote: ... primary B cells were isolated from Rif1FH/FH and C57BL/6 (Rif1WT/WT) mice spleens using anti-CD43 MicroBeads (Miltenyi Biotec) and expanded in complete RPMI containing 5 µg/ml LPS (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue was disrupted by running program m_lung_01 on the gentleMACS dissociator (Miltenyi Biotec) followed by 30 min incubation at 37°C with continuous shaking ...
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bioRxiv - Genetics 2023Quote: ... supernatant was taken and then filtered using a 30 μm cell strainer (Miltenyi Biotec, 130-041-407) into a nuclease free collection tube ...
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bioRxiv - Genomics 2023Quote: ... the tissue was chopped into small pieces with scissors and then transferred along with the lysing buffer into a gentleMACS C tube (Miltenyi Biotec, Cat. 130-096-334). An additional 1 ml of lysing buffer was added into the C tube to make a final volume of 2 ml ...
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bioRxiv - Genomics 2023Quote: ... 2 mM EDTA; dilution of MACS BSA Stock Solution [Miltenyi Biotec, 130-091-376] 1:20 with autoMACS® Rinsing Solution [Miltenyi Biotec, 130-091-222]).
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bioRxiv - Genomics 2023Quote: Singlet rate was estimated on dissociated tissues using flow cytometry on a MACSQuant Analyzer 10 (Miltenyi Biotec, 130-096-343). Flow cytometry data was analyzed using Flowlogic v8 (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2023Quote: ... and cells were washed and labeled with the following antibodies for 30 min at 4 °C: rat anti-CD45 PerCP/Cyanine5.5 (1:100, catalog no. 130-102-469; Miltenyi Biotec); rat anti-SiglecH PE (1:100 ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.4 x 106 isolated T cells from the same donor and 0.4 x 106 T cell activation beads (Miltenyi Biotec, anti-CD2/3/28) were added ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary human monocytes and CD3+ T cells were isolated from PBMCs using CD14 MicroBeads™ and pan T cell isolation kit (Miltenyi Biotec), respectively ...
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bioRxiv - Molecular Biology 2023Quote: PBMCs and CD14-depleted PBMCs were cultured in 96-well u-bottom plates at a density of 5 × 105 per well and stimulated with anti-CD2/CD3/CD28-coated T-cell activation/expansion beads (Miltenyi Biotec) in the presence of IL-2 (20 ng/ml ...
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bioRxiv - Cell Biology 2023Quote: ... cut longitudinally into roughly 2-mm-long pieces and were isolated using the Liver Dissociation Kit (mouse) and GentleMACS Dissociator (Miltenyi Biotec, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... cell surface-stained cells were fixed for 30 min at room temperature with Foxp3 fixation/permeabilization buffer (Thermo Fisher Scientific, 00-5523-00 or Miltenyi Biotec, 130-093-142). After a washing step with FACS buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were filtered to remove dead cells and B-cell depletion was performed using human CD19 microbead (Miltenyi Biotec) labeling according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... SiglecF-positive cells were enriched using anti-PE MultiSort Kit (Miltenyi Biotec). 1x105 flow through and AM enriched fractions were co-cultured with 1x105 hCD1Tg BMDCs and 3x105 DN1 T cells for 48 hours and cells were stained for the expression of activation markers.
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bioRxiv - Systems Biology 2023Quote: ... supplemented with growth factors (Miltenyi Biotec, IL-3, IL-6 and G-CSF (10 ng/mL), h-SCF and FLt3-L (50 ng/mL) ...
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bioRxiv - Molecular Biology 2023Quote: ... using the autoMAC separator (Miltenyi Biotec) according to the manufacturer’s description ...
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bioRxiv - Cell Biology 2023Quote: ... biotinylated TU-tagged RNA was separated from untagged RNA using the µMacs Streptavidin kit (Miltenyi Biotec 130-074-101). High salt MPG buffer (100 mM Tris ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched CD4+ T cells were stained with fluorescent antibodies against CD4 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ memory T cells were enriched using the negative selection magnetic-activated cell sorting CD4+ T cell isolation kit II (Miltenyi Biotec, San Diego, CA). Enriched cells were stained with anti-CD4 ...