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Citations for Miltenyi Biotec :
2651 - 2700 of 3164 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... then the cells were separated into bound and non-bound fractions on an autoMACS™ separator column (Miltenyi Biotec GmbH). The purity of the isolated cells ...
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bioRxiv - Molecular Biology 2019Quote: ... CD14+ cell isolation was done using immunomagnetic bead selection according to manufacturer’s instructions to achieve 95–99% purity (Miltenyi Biotec). CD14+ monocytes were cultured in RPMI cell medium supplemented with 10% FCS ...
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bioRxiv - Cell Biology 2020Quote: ... prior to separation into CD34+ and flowthrough (CD34−) cells using magnetic beads (CD34 Microbead Kit: Miltenyi Biotec, 130-046-702).
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bioRxiv - Immunology 2020Quote: ... A pool of CD4+ and CD8+ T cells was isolated using CliniMACS CD4 and CD8 GMP MicroBeads (Miltenyi Biotec, Germany). GMP-grade lentiviruses ...
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bioRxiv - Systems Biology 2019Quote: ... after which the cells were incubated with streptavidin-coated magnetic beads and then passed through a magnetic column (Miltenyi Biotec). The eluted DN cells were either used directly for RNA-FISH analysis or further purified by staining with fluorescent antibodies to CD44 ...
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bioRxiv - Immunology 2021Quote: ... the sample was subjected to a “clean-up” step using Dead Cell Removal kit (Miltenyi Biotec, Catalog #130-090-101). Cells were washed again and resuspended in 500 ul of 0.04% BSA/PBS and counted ...
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bioRxiv - Immunology 2020Quote: ... PBMCs were depleted of B and NK cells via CD19+ and CD56+ microbeads according to the manufacturer’s protocol (Miltenyi Biotec). M1- and M2- macrophages were generated from blood monocytes in vitro ...
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bioRxiv - Immunology 2020Quote: ... for 30 minutes at 37°C and dissociated through a 30-mm pore cell strainer (Miltenyi Biotec, Bergisch Gladbach, Germany). For chemokine binding assays ...
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bioRxiv - Cell Biology 2019Quote: ... Monocyte-derived dendritic cells (MDDCs) were generated from plastic adherent or directly isolated monocytes (Monocyte Isolation Kit II, Miltenyi Biotec) through culture for 6 days in Roswell Park Memorial Institute medium (RPMI-1640 ...
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bioRxiv - Immunology 2019Quote: ... WT mouse by depleting T cells from the spleen using the CD3ε microbead kit according to the manufacturer’s instructions (Miltenyi Biotec). T cells were stained with CellTrace Violet as described in the above section and plated in 96-well flat-bottom plates at 1.5×105 T cells per well with/without 2×104 antigen presenting cells isolated from naïve WT spleen ...
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bioRxiv - Immunology 2021Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2021Quote: ... OT-1 cells were purified from OT-1 transgenic mice using negative selection with CD8a+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Cell Biology 2020Quote: ... was purified using magnetic-activated cell sorting to deplete the supernatant of CD45+ cellular debris (Miltenyi Biotec, Bergisch Gladbach, Germany)36,37.
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bioRxiv - Immunology 2021Quote: NK cell effectors were enriched from normal cynomolgus macaque (Macaca fascicularis) blood samples using a CD3 depletion Kit (Miltenyi Biotec). Target and effector cells were plated at E:T ratio of 2:1 in a 96 well round bottom plate ...
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bioRxiv - Immunology 2020Quote: ... cells were harvested from femur and tibiae of 6–9 week-old CD11c-DTR or CD11c-DTR Rag2−/− SJL mice and depleted of T cells using anti-CD3 and anti-CD8 magnetic beads according to the manufacturer’s instructions (Miltenyi Biotec). Wild type or Rag2−/− SJL mice were gamma-irradiated with 950 rads from a 60Co source ...
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bioRxiv - Developmental Biology 2021Quote: ... with the program “possel.” Cells in the flow-through fraction were washed with MACS buffer and incubated with CD90.2 (THY1) MicroBeads (Miltenyi Biotec) on ice for 20 min to remove THY1-positive spermatogonia ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated from spleen of P2Y6fl/fl and P2Y6ΔNcr1 mice fed a normal chow diet using the mouse NK cell isolation kit from Miltenyi Biotec, (Bergisch Gladbach ...
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bioRxiv - Immunology 2021Quote: ... and monocytes were separated from lymphocytes by positive selection using magnetic cell sorting technique according to the manufacturer’s instructions (Miltenyi Biotec) and as described (34).
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bioRxiv - Immunology 2020Quote: ... spleen cells were depleted for CD4 and CD8 T cells using magnetic bead depletion techniques according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... Cells were rinsed once with media and then CD3+ T-cells were isolated via positive magnetic selection or pan T-cells were isolated via negative selection according to the manufacturer’s protocol (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... 8-12-week-old mice were intraperitoneally injected with 10 million CD4+ T cells (Miltenyi Biotec, Cat#130-104-454) from Bm12 mice purified by magnetic cell sorting and mice were sacrificed for analysis at indicated time points ...
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bioRxiv - Immunology 2020Quote: ... Lineage cells (Ter119+ F4/80+ Gr1+) were depleted using magnetic anti-biotin Microbeads (1:5) (Miltenyi Biotec 130-090-485) and MS columns (Miltenyi Biotec 130-042-201) ...
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bioRxiv - Immunology 2020Quote: ... CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic bead selection (Miltenyi Biotec, San Diego, CA, USA). To differentiate cells into macrophages ...
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bioRxiv - Immunology 2022Quote: ... pan splenic T cells were isolated from crushed spleens of naïve C57BL/6 mouse using the Mouse Pan T Cell Isolation Kit (Miltenyi Biotec). In the subsequent co-culture incubation ...
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bioRxiv - Cancer Biology 2022Quote: CD3+ T cells from healthy donors were separated from PBMCs using CD3 immunomagnetic beads (#130-097-043, Miltenyi Biotec, Germany), then amplifing using CD3/CD28 stimulation beads (#11131D ...
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ADAR2 deaminase activity promotes Th17 effector function and protects against intestine inflammationbioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2022Quote: ... cells were prepared from CD45.1 Thy1.2 C57Bl/6 mice by first crushing bones followed by T cell depletion using CD4 and CD8 MicroBeads (Miltenyi Biotec). CD45.2 Thy1.2 FoxP3/GFP+ CD4+ Treg were FACS sorted from CD8/CD19-depleted single cell suspensions from spleens and lymph nodes from CD45.2 Thy1.2 FoxP3GFP+ C57Bl/6 using a BD FACS Aria II ...
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Stromal transdifferentiation drives lymph node lipomatosis and induces extensive vascular remodelingbioRxiv - Pathology 2022Quote: ... CD45+ immune cells were depleted from the samples using CD45 beads and LS-columns following the manufacturer’s instructions (Miltenyi Biotec). The pellets were resuspended in Fc block (1:100 in FACS buffer containing EDTA and fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in sorting buffer and incubated with ACSA-2 MicroBeads (1:10, Miltenyi Biotec, 130-097-678) for 15 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the lung draining lymph nodes (DLN) and spleens were harvested and donor T cells were isolated using MACS (Miltenyi Biotec) based on their congenic marker (CD90.1) ...
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bioRxiv - Immunology 2022Quote: Mouse naive T cells were purified from spleens and lymph nodes of 8-12 weeks old mice using the Naive CD4+ T Cell Isolation Kit according to the manufacturer’s instructions (Miltenyi Biotec). Cells were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Immunology 2022Quote: ... The pellet was resuspended in 0.04% BSA and strained through MACS 30µm Cell Strainer (Miltenyi Biotec Ltd 130-041-407) twice ...
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bioRxiv - Immunology 2024Quote: ... IMhu-M cells were cultivated in IL-4/GM-CSF MoDC differentiation medium (Miltenyi Biotec, Cat. No. 130-094-812), when indicated ...
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bioRxiv - Pathology 2024Quote: ... Cultures were negatively immunoselected for hematopoietic cells using CD45 and CD11b microbeads (Miltenyi Biotec, 130-052-301 & 130-097-142) and the autoMACS Pro Separator following the manufacturer instructions ...
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bioRxiv - Genomics 2024Quote: The obtained homogenate was filtered in 3-5 ml of Wash Buffer (1x DPBS, containing 10% FBS, 20mM HEPES, and 6mM glucose) through a prewetted 70μm cell culture filter (Miltenyi Biotec) and centrifuged for 5 minutes at 300xg (4 °C).
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bioRxiv - Immunology 2024Quote: IFN-γ production of CD8+ T cells was measured by adapting a commercially available IFN-γsecretion assay kit (Miltenyi Biotec). Briefly ...
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bioRxiv - Immunology 2024Quote: ... resuspended at 4 mio cells/ml and then cultured with HLA class I-enhanced neurons (see Human-iPSC-derived neuron preparation section above) at a density of 1.2mio cells/cm2 with IL-2 (1000UI/ml, Miltenyi Biotec) and a CD28 agonist antibody (5ug/ml ...
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bioRxiv - Cell Biology 2023Quote: CD34+ magnetic cell isolation was performed on the PBMCs according to the manufacturer’s protocol for the Direct CD34+ progenitor cell isolation kit (Miltenyi Biotec), to enrich for haematopoietic progenitor cells ...
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bioRxiv - Microbiology 2023Quote: ... and single-cell suspension was prepared using the mouse lung dissociation kit (Cat: 130-095-927; Miltenyi Biotec, Gaithersburg, MD) and GentleMACS™ Dissociator treatment following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cortices were dissociated into a single cell suspension using a neural tissue dissociation kit (Miltenyi Biotec; 130-092-628). Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed in a 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Microbiology 2023Quote: ... cells were permeabilized and stained in buffer from commercial kit (Inside Stain Kit, ref no. 130-090-477, Miltenyi Biotec) for 40 minutes at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Hearts were mashed with pestles for 2 min and cell isolation performed according to manufacturer’s instructions (Neonatal Heart Dissociation Kit, Miltenyi Biotec). Cell suspension was centrifuged for 5 min at 600 x g and cells were resuspended in cell culture medium and plated in 22.1 mm plates ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were then obtained using a negative magnetic bead-based selection kit for pan T-cell isolation following the manufacturer’s protocol (Miltenyi Biotec). A negative selection was chosen to minimize any activation of T-cells during the selection process ...
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bioRxiv - Developmental Biology 2023Quote: ... The flow-through was then subjected to MuSC enrichment using the satellite cell isolation kit (Miltenyi Biotec 130-104-268). Once each population was isolated ...
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bioRxiv - Microbiology 2023Quote: ... Cells from the colonic lamina propria (cLP) were isolated using a Lamina Propria Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) on the gentleMACS Dissociator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... 8.3 CD8 T cells were isolated from the LNs of non-diabetic NY 8.3 NOD female mice using two rounds of the CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: Naïve CD4+ T cells were purified from spleen and lymph nodes of 6-15 week old 2D2 mice or OT-II mice using the naïve CD4+ T cell isolation Kit (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD4 naïve T cells were isolated from pooled lymph nodes and spleens using the naïve CD4 T cell isolation kit (130-104-453, Miltenyi Biotec) and stained with BioTracker 655 Red Cytoplasmic Membrane Dye (SCT108 ...
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bioRxiv - Immunology 2023Quote: ... tumour suspensions were mashed through a 70 μm filter and live cells were enriched using debris removal solution (Miltenyi Biotec). For obtaining single cell suspensions for the KM macrophage datasets ...