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Citations for Miltenyi Biotec :
2551 - 2600 of 3164 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... brain cells were isolated using a 30%/80% Percoll gradient after homogenizing tissue on a gentleMACS dissociator (Miltenyi Biotec). Single cell suspensions were then incubated with purified anti-CD16/32 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... blood and peritoneal cavity cells were stained using the following antibodies: CD3 PerCP-Vio770 (130-119-656, Miltenyi Biotec), CD4-APC (130-123-207 ...
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bioRxiv - Cancer Biology 2024Quote: ... Depletion of CD45+ cells in NSCLC samples was performed using CD45 MicroBeads (Miltenyi Biotec, cat. no. 130-045-801) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... the unbound complexes were washed and the cells processed through magnetic columns (Miltenyi Biotec, Catalog No. 130-042-401) following manufacturer instruction ...
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bioRxiv - Immunology 2024Quote: ... Spleen qPCR samples were prepared by depletion of CD19+ TCRβ+ cells using biotinylated antibodies and Streptavidin MicroBeads (Miltenyi Biotec) according to the manufacturer’s instructions ...
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T-cell development and activation in humanized mice lacking mouse major histocompatibility complexesbioRxiv - Immunology 2024Quote: ... CD34+ cells were positively selected through two consecutive runs using immune magnetic beads kits (Miltenyi Biotec, Bergisch-gladbach, Germany) and cryopreserved ...
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bioRxiv - Immunology 2024Quote: ... synchronisation at trophozoite or schizont stage was performed by utilizing magnetic activated cell sorting (Miltenyi Biotec MACS LD columns).
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bioRxiv - Cell Biology 2024Quote: For single-cell sequencing experiments samples were additionally run on a gentleMACS™ Dissociator (Miltenyi Biotec, #130-093-235) with the custom program h_skin_01 (Duration ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were pelleted and resuspended in TMRE buffer and subjected to flow cytometry on a MACSQuant Analyzer (Miltenyi Biotec). For mitochondrial superoxide measurements ...
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bioRxiv - Immunology 2023Quote: hCD8+ T cells were isolated from commercially available sources (buffy coats from ZenBio Inc. and leukopaks from Miltenyi Biotec) using magnetic separation (positive selection ...
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bioRxiv - Immunology 2023Quote: ... Leukocytes were isolated from whole blood by hypotonic lysis of erythrocytes using Red Blood Cell Lysis Solution (Miltenyi Biotec) followed by ice cold PBS/1% BSA/2mM EDTA washes ...
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bioRxiv - Genetics 2022Quote: ... The following antibodies were used for cell surface staining: IgA (clone IS11-8E10) (Miltenyi Biotec, cat#: 130-114-002), IgD (clone IA6-2 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then pelleted and resuspended in 5 mL PBSF and sorted using a MidiMACS Separator magnet (Miltenyi Biotec) in combination with an LS column (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2022Quote: ... and dead cell removal following the manufacturer’s protocols (Miltenyi Biotec, 130-095-929, 130-104-694, 130-090-101). Suspensions of single cells were processed on a Fluidigm C1 instrument using a C1 Single-Cell mRNA Seq HT IFC and Reagent Kit v2 (Fluidigm ...
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bioRxiv - Immunology 2023Quote: In vitro cytotoxicity assays were performed by co-culturing sorted CD8 T-cells (Miltenyi Biotec catalogue # 130-104-075) with tumor cells in the presence or absence of antigen-pulsed macrophages ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspensions from tumor tissues were prepared using the Mouse Tumor Dissociation Kit (Miltenyi Biotec, Auburn, CA, USA) and Percoll density gradient separation according to the manufacturer’s recommendations (GE Healthcare ...
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bioRxiv - Cancer Biology 2023Quote: ... Spleen single-cell suspensions were generated by using the gentleMACS tissue dissociator with Gentle MACS tubes C (Miltenyi Biotec). Single cell suspensions from LNs were prepared by grinding the tissue through 70 mm cell strainers (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cell concentrates were enriched in CD34+ HSPCs using a CliniMACS Plus instrument (Miltenyi Biotec, Gaithersburg, MD, USA) and cryopreserved prior to use ...
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bioRxiv - Neuroscience 2023Quote: ... and processed to create a single-cell suspension using the Adult Brain Dissociation Kit (#130-107-677, Miltenyi Biotec) supplemented with Actinomycin D (#A1410 ...
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bioRxiv - Immunology 2023Quote: ... CD45.2+ splenic B cells from SWHEL donor mice were purified using anti-mouse CD43 (Ly-48) MicroBeads (Miltenyi Biotec). If not specified ...
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bioRxiv - Neuroscience 2023Quote: ... Analyses of the labeled cells were conducted on a MACSQuant Analyzer 10 Flow Cytometer (#130-096-343, Miltenyi Biotec). The proportion of eGFP+ cells that co-labelled with CD11b+CD45+ was assessed and compared between groups using MACSQuantify v2.13.0 software.
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bioRxiv - Biophysics 2023Quote: ... and resting mouse B-cell isolation kit (Cat# 130-090-862) were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany). Oligonucleotides were obtained from Integrative DNA Technologies (Coralville ...
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bioRxiv - Cell Biology 2023Quote: ... The mononuclear cells were collected and incubated with CD45 magnetic microbeads (130-045-801; Miltenyi Biotec, Bergisch Gladbach, Germany) for 30 minutes at 4℃ in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... Analysis for plasma- and cell line-derived EV samples was performed using the MACSQuant® Analyzer 16 (Miltenyi Biotec) and the corresponding software MACSQuantify™ (Version 2.13.3) ...
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bioRxiv - Immunology 2023Quote: ... Further purification of immune cells was done by magnetic enrichment of the CD45+ fraction (130-052-301, Miltenyi Biotec). All cell suspensions were kept in PEB buffer (0.5% BSA and 2 mM EDTA in PBS).
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bioRxiv - Neuroscience 2023Quote: Cells were isolated from the adult brain using the Adult Brain Dissociation kit (Miltenyi Biotec, cat # 130-107-677) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed twice with serum free medium prior to FACS analyses (MACSQuant Analyzer, Miltenyi Biotec, Bergisch Gladbach, Germany). A forward- and side-scatter gate was set to exclude dead cells with forward-scatter (FSC ...
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bioRxiv - Cancer Biology 2023Quote: ... after which CD34+ stem cells were isolated by MACS sorting using CD34 MACS microbeads (Miltenyi Biotec, Leiden, The Netherlands) following manufacturers recommendations ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated for 10 min with 25 µl of FcR Blocking Reagent (Miltenyi Biotec 130-059-901) (pre-diluted 1:50 with FACS buffer (PBS ...
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bioRxiv - Immunology 2023Quote: CD4+ T cells were isolated from spleens and non-pancreatic draining lymph nodes (ndLN) by MACS enrichment (Miltenyi Biotec). Treg and Teff cells were then sorted from CD4+ T cells (Teff ...
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bioRxiv - Immunology 2023Quote: ... Splenocytes of P2-TCRα retrogenic mice were enriched for CD4+ cells using CD4 microbeads (Miltenyi Biotec, 130-117-043). Teffs (≤ 140,000 cells per mouse ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with 10 ml of MACS buffer and loaded on LS columns (Miltenyi Biotec, 130-042-401) on the magnetic stand ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The tumor samples were dissociated into single cells using GentleMACS tissue dissociator and its kit (Miltenyi Biotec, Gladbach, Germany). The tumor samples were cut into small pieces ...
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bioRxiv - Cell Biology 2023Quote: Cells expanded following patient biopsy collection were subsequently enriched for myoblasts using anti-CD56-coated microbeads (Miltenyi Biotec GmbH). Primary vials were sourced ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspension was used for the isolation of neurons using the Adult Neuronal Isolation kit (Miltenyi Biotec) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Liver tissue was dissociated by following the ‘preparation of single cell suspensions from mouse liver’ protocol from Miltenyi Biotec. Cells were then washed in cold PBS+ 2% FBS buffer before staining ...
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bioRxiv - Developmental Biology 2023Quote: Cell suspension underwent two negative selection steps to deplete myelin (Myelin removal beads II, 130-096-733, Miltenyi Biotec) and CD45-positive cells (CD45 microbeads ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells labeled with APC-Cy7 were depleted by using anti-APC magnetic beads (Miltenyi Biotec, Ref#130-090-855) with auto MACS pro (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2023Quote: Astrocytes were isolated by magnetic assisted cell sorting (MACS) approach using the anti-ACSA2 microbeads (Miltenyi Biotec Inc., Germany). Briefly ...
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bioRxiv - Immunology 2023Quote: ... or anti-CD3 mAb (0.5 μg/mL) with T-cell depleted [CD90.2 depletion by the LD Columns (Miltenyi Biotec)] and Mytomycin C (KYOWA KIRIN)-treated (or non-treated ...
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bioRxiv - Cell Biology 2023Quote: ... were used to deplete lineage+ (Lin+) cells and Anti-Sca-1 (non-HSC) MicroBeads (130-106-641, Miltenyi Biotec) for mice for enriching with Sca+ cells ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed to remove unbound tetramer and were incubated with anti-PE or -APC microbeads (Miltenyi Biotec, Germany) for 15 minutes on ice ...
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bioRxiv - Genetics 2023Quote: ... The single-cell suspensions of the clinic tumor tissues mentioned above were prepared by Tumor Dissociation Kit (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: SIY-reactive CD8+ T cells were isolated from the spleen and lymph nodes from TCR-transgenic 2C CD45.1+ or 2C CD45.2+ Rag2-/- mice using the CD8a+ T cell isolation kit (Miltenyi Biotec), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: We dissociated cells with Trypsin-EDTA or with the Embryoid Body dissociation kit (cod. 130-096-348 Miltenyi Biotec). Dissociated cells (1×106 cells/100 μl ...
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bioRxiv - Immunology 2023Quote: We prepared B cell tetramers of biotinylated S2hlx-Ex19 or FP-10 by mixing with Streptavidin-VB515 (Miltenyi Biotec) and Streptavidin-AlexaFluor 647 (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... tumor cells were incubated 24 hours in R10 medium (i.e. supplemented or not with 200 ng·mL-1 IFNγ (Miltenyi Biotec)) ...
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bioRxiv - Genetics 2023Quote: ... and enriched for iPSC-RPE via magnetically activated cell sorting using anti-CD24 and anti-CD56 antibodies (Miltenyi Biotec). iPSC-RPE cells in the negative cell fraction were seeded on the desired surface pre-coated with Laminin (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... CD45+ cells were positively selected from the tumor digest with Miltenyi LS columns (Miltenyi Biotec, cat. 130-042-401) and MidiMACS separator (Miltenyi Biotec ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: Naïve B cells (CD43-B220+CD3-) were obtained by negative selection (mouse CD43 Ly-48 MicroBeads; MACS Miltenyi Biotec) from spleens of WT and Lrba-/- mice ...