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Citations for Miltenyi Biotec :
2451 - 2500 of 3164 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... Total splenic B cells (CD19+) were purified using CD19 MicroBeads (130-121-301, Miltenyi Biotec, San Jose, CA), and CD19+CD43- non-B-1 cells were purified using Mouse B Cell Isolation Kit (130-090-862 ...
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bioRxiv - Biochemistry 2022Quote: ... Separation of yeast populations was done via magnetic-activated cell sorting (Miltenyi Biotec, cat. no. 130-091-051) using either streptavidin (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... cells were washed and labeled with CD154-Biotin followed by anti-Biotin MicroBeads (CD154 MicroBead Kit; Miltenyi Biotec) and magnetically enriched by two sequential MS columns (Miltenyi Biotec) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed twice with 1X assay buffer before being assessed by a MACSquant analyzer (Miltenyi Biotec, Germany) with a green fluorescence channel using a 488 nm laser source ...
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bioRxiv - Microbiology 2024Quote: ... Purity of CD4+ T cell population was measured by flow cytometry (MACSquant Analyzer using MACSQuantify software, Miltenyi Biotec), showing a fraction of at least 95% CD4+CD3+ double positive cells and less than 0.01% CD123+CD304+CD303+ cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells in DMEM+FBS were subsequently filtered through a 70µM filter (Miltenyi Biotec, Cat-Nr: 130-098-458) and transferred to 15ml conical Falcon tubes ...
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bioRxiv - Molecular Biology 2024Quote: ... Schizonts were isolated for imaging using magnetic purification (102) with a magnetic cell fraction column (MACS, Miltenyi Biotec) and a MidiMACS separator ...
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bioRxiv - Immunology 2024Quote: ... The cells were isolated from the colon lamina propria using the “Lamina Propria Dissociation Kit” from Miltenyi Biotec according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... ∼30h after transfection cells were submitted to MACS column selection using MS columns (130-042-201, Miltenyi Biotec) and LNGFR MicroBeads (130-091-330 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were cultured in medium supplemented with 5 μM of the ROCK inhibitor StemMACS™ Y27632 (Miltenyi Biotec). The differentiation of hiPSCs into midbrain neurons was performed as previously described (35) ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were then incubated with anti-APC magnetic beads (Miltenyi Biotec, Catalog No. 130-090-855, 1/4) for 20 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... and 2 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...
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The T-cell niche tunes immune function through modulation of the cytoskeleton and TCR-antigen forcesbioRxiv - Bioengineering 2024Quote: ... CD3+ T-cells were isolated using negative selection according to the manufacturer’s protocol (Miltenyi Biotec, # 130-095-130). T-cells were then resuspended in RPMI + 10% FBS and 200,000 cells were seeded per tension probe surface ...
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bioRxiv - Cancer Biology 2024Quote: ... The tumors were dissociated into single-cell suspension using the tumor dissociation kit (Miltenyi Biotec, 130-095-929) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... rested overnight at a density of 1×107 cells/mL in 100 IU/mL IL-2 (Miltenyi Biotec)-supplemented medium ...
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bioRxiv - Cancer Biology 2022Quote: ... CD34+ cells were isolated using the CD34 MicroBead Kit or CliniMACS (catalogue no. 130-100-454; Miltenyi Biotec). Patients gave informed consent in accordance with the Declaration of Helsinki and with approval of the National Health Service Greater Glasgow Institutional Review Board and Clyde Institutional Review Board ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared cell pellets were resuspended in 80 µl of AstroMACS separation buffer (Miltenyi Biotec, 130-117-336) per 107 total cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellets were resuspended and subjected to Miltenyi debris removal reagent (cat # 130-109-398, Miltenyi Biotec Inc) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The dissociated single-cell suspension was resuspended and incubated in FcR Blocking Reagent (130–097-678; Miltenyi Biotec) at 4 °C for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Approximately 1 x 106 RFP+ cells were collected in 0.04% BSA in PBS (Miltenyi Biotec 130-091-376) for single-nuclei isolation and multi-ome ATAC/sequencing (10X Genomics) ...
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bioRxiv - Developmental Biology 2023Quote: ... These samples were further processed using Debris Removal Solution and Dead Cell Removal Kit (Miltenyi Biotec., Gaithesburg, MD). Red blood cell depletion from cell suspensions were performed using anti-Mouse Ter-119 antibody (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: Splenic naïve B-cells were prepared using the CD43 negative selection commercial kit (Miltenyi Biotec.; 130-090-862). Spleens were pulled from 2-3 sacrificed sex- and age- matched animals mechanically disintegrated and filtered through a 70 µm cell strainer to prepare a single cell suspension essential for microbead separation ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were resuspended in 90µL of a buffer comprised of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB ...
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bioRxiv - Immunology 2023Quote: ... Cells harboring the receptor were enriched by using biotinylated anti-EGFR antibody and anti-biotin microbeads (Miltenyi Biotec).
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bioRxiv - Immunology 2023Quote: ... EGFRt-positive T cells were enriched using biotin-conjugated anti-EGFR mAb and anti-biotin beads (Miltenyi Biotec), and subsequently expanded using a rapid expansion protocol based on CD3-TCR-activation via OKT-3 (Miltenyi Biotec ...
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bioRxiv - Cell Biology 2023Quote: Monocyte-derived dendritic cells and monocytes isolated with the CD14 MACS kit from Miltenyi biotec (130-050-201) were collected in a V-bottom plate at 105 cells/well (Thermo scientific # 10462012 ...
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bioRxiv - Immunology 2023Quote: ... Bead-bound cells were positively selected through a magnet-mounted MACS LS column (Miltenyi Biotec; 130-042-401). In some experiments ...
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bioRxiv - Immunology 2022Quote: Whole mouse lungs were digested and single cell suspension was prepared using dissociation kit (Miltenyi Biotec, Auburn, CA) as per the vendor’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Flow cytometry analysis were conducted using BD LSR Fortessa™ cell analyzer or MACSQuant® analyzer (Miltenyi Biotec). Data were prepared using FlowJo 10.1 (Tree Star ...
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bioRxiv - Cancer Biology 2023Quote: ... GCB cells were first enriched from mouse splenocytes using the PNA MicroBead Kit (Miltenyi Biotec, 130-110-479), followed by antibody incubation and sorting on BD Aria II sorter (RRID:SCR_018091 ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8 T cells were isolated from the LNs of NY 8.3 NOD mice using two rounds of the naïve 8.3 CD8 T cell isolation kit (Miltenyi Biotec) with LS columns (Miltenyi Biotec) ...
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bioRxiv - Physiology 2023Quote: Single-cell suspensions were prepared from mouse lungs using the Lung Dissociation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... germ cells were recovered using an anti-SSEA-1 antibody conjugated to MicroBeads (Miltenyi Biotec, 130-094-530) in accordance with the manufacturer’s instructions.
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... FLK1 expressing cells were sorted for by separation using a MACS LS column (Miltenyi Biotec, 130-042-401). FLK1 expressing HB cells were then taken for flow cytometry analysis and sequencing experiments as well as further differentiation in blast culture into HE1 ...
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bioRxiv - Cancer Biology 2023Quote: ... red blood cells were removed from the samples using RBC lysis reagent (Miltenyi Biotec, cat.no.130-094-183). After RBC lysis ...
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bioRxiv - Molecular Biology 2023Quote: ... and stimulated and expanded with the T Cell Activation/Expansion Kit (Miltenyi Biotec, Cat No. 130-091-441) according to the vendor’s manual ...
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bioRxiv - Biochemistry 2023Quote: Naïve CD4+ T cells were isolated by negative selection from mouse spleens using the isolation kit (Miltenyi Biotec) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2023Quote: ... CD11b-positive brain cells were selected from the brain with microbeads-conjugated CD11b antibody (clone M1/70.15.11.5; Miltenyi Biotec) and CD4-positive spleen cells from the spleen with Dynabeads magnetic beads-conjugated CD4 antibody (clone L3T4 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were suspended in 90µL of a buffer made of autoMACS Rinsing Solution (Miltenyi Biotec, #130-091-022) with 0.5% BSA (BB) ...
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bioRxiv - Neuroscience 2023Quote: ... OPC magnetic cell sorting was performed using anti-O4-coupled-beads (O4 MicroBead Kit, Miltenyi biotec, Paris, France) and MultiMACS Cell24 Separator Plus (Miltenyi biotec ...
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bioRxiv - Immunology 2023Quote: ... the enriched CD8+ T cells were stimulated with the CMVpp65 overlapping peptide pool PepTivator® CMVpp65 (Miltenyi Biotec) at a concentration of 1 µg of each peptide/ml for 4 hours ...
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bioRxiv - Immunology 2023Quote: ... and CD8+ T cells were purified using a magnetic bead separation kit (Miltenyi Biotec, Cat #130-104-075). Rag1-/- mice were reconstituted with CD8+ T cells by intravenous route (3 x 106 cells/mouse ...
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bioRxiv - Immunology 2023Quote: ... The splenocyte and tumor cell suspensions were then fractionated using CD8 (TIL) microbeads (Miltenyi Biotec, 130-116-478) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and B cells were isolated by negative selection directly from whole blood using MACSxpress® separator (Miltenyi Biotec) with Whole Blood B Cell Isolation Kit (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... Fixed cells were incubated with APC-conjugated cytochrome c antibody (1: 50 dilution in permeabilisation buffer)(Miltenyi Biotec) and analyesd by FACS on an LSR-II flow cytometer (BD Biosciences).
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bioRxiv - Cancer Biology 2023Quote: ... The cell pellets were resuspended with permeabilization buffer and incubated with 20 μl FcR blocking reagent (Miltenyi Biotec) for 10 min at 4°C in the dark ...
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bioRxiv - Cancer Biology 2023Quote: For CD44 staining gliomaspheres were dissociated into single cells and stained with anti-CD44-APC antibody (Miltenyi Biotec) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... PBMCs were depleted of CD14 mononuclear cells using anti-CD14 microbead antibodies (cat#130-050-201, Miltenyi Biotec) and the flowthrough was enriched for CD4+ T cells by positive selection using anti-CD4 microbead antibodies (cat#130-045-101 ...
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bioRxiv - Bioengineering 2023Quote: ... This was followed by the isolation of CPM+ lung progenitor cells using an autoMACS Pro Separator (Miltenyi Biotec) for the subsequent experiments.