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Citations for Miltenyi Biotec :
2401 - 2450 of 3163 citations for Primary Human Arterial Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... CD14+ cells were positively selected from PBMC using anti-CD14-coated magnetic beads (Miltenyi Biotec, Bergisch Gladbach, Germany). Then cells were cultured in 24-well plates (5×105 cells/mL) ...
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bioRxiv - Developmental Biology 2021Quote: ... the population of undifferentiated neonate spermatogonia cells were separated using CD90.2 (THY1.2+) MicroBeads (Miltenyi Biotec 130-121-278). Relative enrichment of cell populations was evaluated by STRA8 (c-Kit fractions ...
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bioRxiv - Cancer Biology 2020Quote: ... After washing with cold MACS buffer stained cells were incubated with anti-biotin-microbeads (Miltenyi Biotec, Auburn, CA) for an additional 15 min and subsequently subjected to positive selection of Gr-1+ cells on a MACS LS column according to the manufacturer’s instructions (Miltenyi Biotec).
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bioRxiv - Immunology 2021Quote: ... The isolated T cells were then stimulated with IL-7 and IL-15 (Miltenyi Biotec, Bergisch Gladbach, Germany) at a concentration of 25μg/2L bag of TexMACS media with 3% human AB serum (Innovative Research ...
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bioRxiv - Immunology 2021Quote: ... CD4+ T cells from differently treated mice were isolated using CD4+ microbeads according to manufacturer’s instruction (Miltenyi Biotec). The purity of CD4+ T cells was analyzed by flow cytometry after the staining with anti-CD4 antibody and reported to be > 95% ...
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bioRxiv - Cell Biology 2021Quote: ... freshly isolated PB and BM were initially depleted of lineage positive cells with MACS LD columns (Miltenyi Biotec), as previously described53 ...
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bioRxiv - Cancer Biology 2021Quote: ... EpSCs were isolated using magnetic-associated cell sorting using Anti-Sca-1 microbeads (Miltenyi Biotec, 130-106-641) and a MACS MultiStand system (Miltenyi Biotec ...
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bioRxiv - Systems Biology 2021Quote: ... The cell suspension was first filtered twice: through a 70μm and then through a 30μm (MACS, Miltenyi Biotec) filter and finally centrifuged ...
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bioRxiv - Cancer Biology 2020Quote: For CSR experiments spleen naive B cells were isolated by immunomagnetic depletion using anti-CD43 beads (Miltenyi Biotec). Purified naïve B cells were cultured at 1.2 x 106 cells/ml in complete RPMI medium supplemented with 10% FBS (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: CD3+T-cells were isolated from healthy C57/B6 mice using a Miltenyi kit (Miltenyi Biotec, Auburn, CA). Round bottom plates were coated with 10μg of functional CD3 antibody (eBiosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... cell cycle analysis was carried out by flow cytometry with a MACSQuant 10 or VYB cytometer (Miltenyi Biotec) and analysed with FlowLogic software.
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bioRxiv - Cell Biology 2020Quote: ... Thy1.2 cells were depleted via negative selection using the CD90.2 MicroBeads mouse Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions from whole mouse lungs were prepared using the lung dissociation kit (Miltenyi Biotec, Auburn, CA) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... CD11c+ cells were purified from mouse spleen using anti-CD11c MicroBeads UltraPure and MACS separation columns (Miltenyi Biotec). Purity checks and cell counts were performed using a BD Accuri C6 flow cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... density gradient centrifugation to isolate peripheral blood mononuclear cells (PBMC) and subsequently using CD4 magnetic MicroBeads (Miltenyi Biotec) according to the manufacturers’ protocols ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspension was depleted of microglia and myelin debris (Myelin Removal Kit, 130096733, Miltenyi Biotec, Bergisch Gladbach, Germany), then astrocytes were isolated using the Miltenyi Biotec’s anti-ACSA-2 magnetic cell sorting kit and protocol (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated from the spleen and peripheral lymph nodes using anti-CD4 microbeads (Miltenyi Biotec) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... CD19+ B cells were magnetically removed from freshly isolated PBMCs using CD19 Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany) according to manufacturer’s recommendations (depleted condition) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 1 mL PEB buffer and proceeded to magnetic separation on autoMACSpro separator (Miltenyi Biotec) with Possels program ...
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bioRxiv - Developmental Biology 2021Quote: ... Cortical cells were dissociated by using the Neural tissue dissociation kit (MACS Miltenyi Biotec, #130-092-628 P). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... MuSCs were selected using the Satellite Cell Isolation Kit mouse (130-104-268, Miltenyi Biotec, Bergisch Gladbach, Germany), seeded at 2000 cells/cm2 and grown for 3 days in growth medium (GM ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed in 1% PFA and analyzed by flow cytometry using the MACSQuant Analyzer 10x (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2020Quote: ... MACS-based purification was performed with Myelin Removal Beads II followed by Mouse Cell Depletion kit (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells at the interface were collected and further purified with CD11b+ magnetic microbeads (Miltenyi Biotec, 130-092-636) or CD45 beads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then washed and resuspended in MACS buffer plus 1:5 anti-mouse igG microbeads (Miltenyi Biotec) and rotated at 4°C for a further 15 min ...
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bioRxiv - Physiology 2022Quote: ... samples were resuspended and incubated with 80 μL anti-PE microbeads per 107 cells (Miltenyi Biotec, Cologne, Germany) for 20 minutes at 4°C before Auto MACS cell separation (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: Microglia cells from adult young or old mice were isolated and cultured using immunomagnetic separation (Miltenyi Biotec, Germany). Mice were perfused via the left ventricle with 60 mL of cold saline and collected in HBSS buffer without calcium/magnesium (#14175-05 ...
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bioRxiv - Immunology 2022Quote: ... cells were blocked at 37 C for 15 min with 0.5 μg/mL anti-CD40 mAb (Miltenyi Biotec). A stimulation with an equimolar amount of DMSO was performed as negative control ...
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The transcription factor RUNX2 drives the generation of human NK cells and promotes tissue residencybioRxiv - Immunology 2022Quote: ... density gradient centrifugation followed by Magnetic-activated cell sorting using biotin MicroBead kit (Miltenyi Biotec, Leiden, The Netherlands), according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... both naïve and memory OT-1 CD8 T cell donors were magnetically sorted via magnetic beads (Miltenyi Biotec) with specific antibodies ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells were isolated by negative selection from spleens and lymph nodes (Miltenyi Biotec, #130-104-454) according to manufacturer’s instructions (typically >95% purity) ...
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bioRxiv - Cell Biology 2022Quote: ... ErbB2 (CD34) PE-Vio770 used for FACS sorting of HER2+ stable cells was obtained from Miltenyi Biotec (Germany).
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bioRxiv - Immunology 2022Quote: ... Acquisition of a minimum of 100,000 cells per sample was performed on a MACSQuant 10 analyzer (Miltenyi Biotec). Single-stained controls using mouse Ig/ κ compensation beads (BD Biosciences ...
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bioRxiv - Cancer Biology 2022Quote: ... fresh splenocytes were isolated as previously described and subjected to Pan-T cell magnetic bead isolation (Miltenyi Biotec) according to manufacturer’s protocols ...
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bioRxiv - Immunology 2020Quote: ... The excess of beads was washed out and cells were then separated over an LS-column (Miltenyi Biotec).
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bioRxiv - Immunology 2020Quote: ... and T cells were depleted using Thy1.1 or Thy1.2 positive selection and a magnetic separation kit (Miltenyi Biotec). Cells were counted ...
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bioRxiv - Immunology 2019Quote: ... Resuspended cells from the bone marrow were labeled using biotinylated anti-CD3 antibody and streptavidin microbeads (Miltenyi Biotec). Cells were then resuspended in PBS and 1×106 cells were transferred to each mouse.
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bioRxiv - Immunology 2019Quote: ... The cells were then labelled with magnetic streptavidin microbeads according to the manufacturer’s instructions (Miltenyi Biotec, Auburn, CA). After washing ...
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bioRxiv - Immunology 2021Quote: ... Released cells were collected in the supernatant and filtered through a 70 mm MACS Smart Strainer (Miltenyi Biotec) into a new 15 mL conical tube ...
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bioRxiv - Developmental Biology 2020Quote: ... resuspended pellets to single cells and incubated with Anti-SSEA-1 (CD15) Microbeads (Miltenyi Biotec, 130-094-530). After collected the positive cells ...
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bioRxiv - Immunology 2020Quote: ... Tissue was dissociated into single-cell suspensions with enzymic digestions using a gentleMACS dissociate per protocol (Miltenyi Biotec). Single-cell suspensions were cryopreserved (10% DMSO ...
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bioRxiv - Immunology 2020Quote: ... CD45+ hematopoietic cells were depleted using anti-CD45 magnetic beads by autoMACS with the depleteS program (Miltenyi Biotec). Total mTECs (EpCAM+UEA-1+Ly51lo) ...
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bioRxiv - Immunology 2021Quote: ... TCRβ+CD138- and TCR+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). Depending on the experimental objective ...
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bioRxiv - Immunology 2020Quote: ... Neutrophils were isolated from BM cells by positive selection using the neutrophil isolation kit (Miltenyi Biotec, Auburn, CA). WT and G184S KI neutrophils were stained by Alexa Fluor 568- and Alexa Fluor 488-conjugated anti-mouse Gr-1 antibodies ...
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bioRxiv - Immunology 2021Quote: ... Isolated cells were negatively selected tandemly first with Myelin Removal Beads II (Miltenyi Biotec, Cat# 130-096-731) and second with anti-CD11b MicroBeads (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: Naïve CD4 T cells were isolated from mice spleens and lymph nodes by magnetic bead separation (Miltenyi Biotec) following the manufacturers’ protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Single cell suspension was achieved using the 37_C Multi_tissue_dissociation B program on the gentleMACS octo-dissociator (Miltenyi Biotec). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... 0.5% BSA) and then incubated with 10µL per 106 cells each of IFN-γ Catch Reagent (Miltenyi Biotec), IL-2 Catch Reagent (Miltenyi Biotec) ...
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Activation of innate immune cGAS-STING pathway contributes to Alzheimer’s pathogenesis in 5×FAD micebioRxiv - Neuroscience 2022Quote: Cell samples were fluorescently stained with Anti-ACSA-2-PE (clone: IH3-18A3) (130-102-365, Miltenyi Biotec), examined by a BD FACSCanto II flow cytometer ...
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bioRxiv - Immunology 2022Quote: ... and 5 mM EDTA and then isolated using mouse B cell purification kit (Miltenyi Biotec, 130-090-862) and LS columns (Miltenyi Biotec ...