Labshake search
Citations for Miltenyi Biotec :
1301 - 1350 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... CD25- cells underwent further selection for CD4 using the human CD4+ T cell isolation kit (130-096-533, Miltenyi Biotec) to establish the non-Treg population (CD4+CD25-) ...
-
bioRxiv - Immunology 2024Quote: ... Cell pellets of Treg and non-Treg cells were collected and stored at -80°C directly after isolation or after 72h stimulation using human Treg Suppression Inspector beads (130-092-909, Miltenyi Biotec) in X-VIVO 15 (02-060F ...
-
bioRxiv - Immunology 2024Quote: CD4+ and CD8+ T cells and CD14+ monocytes were isolated from healthy donor mononuclear cells (agreement ANG-2003-2) by positive magnetic sorting (Miltenyi Biotec, Germany). Macrophages were generated by culturing monocytes for 7 days with 50 ng/ml M-CSF or 50 ng/ml GM-CSF (ORF Genetics Kopavogur ...
-
bioRxiv - Immunology 2024Quote: ... kappa Isotype control (Miltenyi Biotec) was injected.
-
bioRxiv - Immunology 2024Quote: ... MACS was performed according to the manufacturer’s instructions using Anti-Mouse IgG Microbeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Cells were stored in RNAlater (Thermo Fisher Scientific Baltics UAB ...
-
bioRxiv - Immunology 2024Quote: ... human (Miltenyi Biotec, USA), following the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... dissected tissue fragments from freshly isolated biopsies were transferred in GentleMACS C tubes (Miltenyi Biotec) containing Hanks’ Balanced Salts Solution (HBSS ...
-
bioRxiv - Immunology 2024Quote: ... and Tconvs (CD4+CD25-) were purified (90-95% pure) by using the CD4+CD25+ Regulatory T Cell Isolation Kit (Miltenyi Biotec).
-
bioRxiv - Immunology 2024Quote: ... then stained with surface antibodies in MACS buffer (MACS wash buffer (Miltenyi Biotec), BSA (Mitlenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... In-vitro CD4 T cell survival assays were performed in complete RPMI medium supplemented with recombinant murine IL-2 (100 ng/ml, Miltenyi Biotec) or IL-7 (10 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... was used to lyse red blood cells before T cell isolation using the naive CD4+ T Cell Isolation Kit from Miltenyi Biotec or the MojoSort™ Mouse CD4 or CD8 Naïve T Cell Isolation Kits from BioLegend ...
-
bioRxiv - Immunology 2024Quote: All tumors were dissociated using the Human Tumor Dissociation kit (Miltenyi Biotec; Cat#130-095-929) within 30 minutes after surgery (Supplementary Methods ...
-
bioRxiv - Immunology 2024Quote: Peripheral blood mononuclear cells were separated by Ficoll-Paque PLUS (GE) density gradient centrifugation from which monocytes were enriched using CD14 magnetic beads (Human Monocyte Isolation Kit human, Miltenyi Biotec, RRID:AB_2665482) and T cells using Human T Cell Isolation Kit (negative selection ...
-
bioRxiv - Immunology 2024Quote: ... or 10 μg/mL plate-bound αPan-γδ TCR antibody (Miltenyi Biotec) and 2 μg/mL soluble αCD28 antibody (ThermoFisher) ...
-
bioRxiv - Immunology 2024Quote: ... lungs of infected mice were harvested and homogenized in 1 mL of minimal essential media with 10% bovine serum albumin using a gentleMACS C Tube (Miltenyi Biotec). Subsequently ...
-
bioRxiv - Immunology 2024Quote: ... and analyzed using Flowlogic™ software (Miltenyi Biotec).
-
bioRxiv - Immunology 2024Quote: ... Splenocytes were loaded into the autoMACS Pro Separator (Miltenyi Biotec, Bergisch Gladbach, Germany) for CD11b+ cell selection ...
-
bioRxiv - Immunology 2024Quote: ... Tissue dissociation was made on a GentleMACS Dissociator (Miltenyi Biotec) by using the “h_tumor 01_03” program ...
-
bioRxiv - Immunology 2024Quote: ... Samples were blocked with FcR blocker (Miltenyi Biotec) prior to staining with the following antibodies for 20 min at 4 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... membranes were probed overnight at 4°C with the corresponding antibodies : anti-GFP-conjugated with HRP (Miltenyi Biotec 130-091-833, 1:5000), anti-RFP (Abcam 34767 ...
-
bioRxiv - Plant Biology 2024Quote: ... Immunoprecipitation was carried out using micromagnetic bead-coupled GFP antibodies from µMACS GFP Isolation Kit (Miltenyi Biotec 130-091-125) following the manufacturer’s instruction ...
-
bioRxiv - Molecular Biology 2024Quote: ... 100 ng/mL FGF-2 (Miltenyi Biotec, #130-093-564), and 100 ng/mL VEGF-A were carefully added to the center of each well ...
-
bioRxiv - Immunology 2024Quote: ... 20ng/ml rmIL-1b (all from Miltenyi Biotec) and 20ng/ml rmIL-23 (R & D systems ...
-
bioRxiv - Immunology 2024Quote: ... naïve CD8+ T cells were purified by magnetic bead separation using mouse naïve CD8a+ T Cell Isolation Kit (Miltenyi Biotec, 130-096-543) following vendor instructions ...
-
bioRxiv - Immunology 2024Quote: ... CD4+ T cells were enriched by MACS (Miltenyi Biotec) and CD40L+4-1BB+ and CD40L-4-1BB- cells FACS sorted using an Aria SORP (BD) ...
-
bioRxiv - Immunology 2024Quote: ... and anti-CD40 (clone HB14, Miltenyi Biotec). CD4+ T cells were enriched by MACS (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... All samples were measured on a MACSQuant® Analyzer 16 (Miltenyi Biotec). Instrument performance was monitored prior to every measurement with Rainbow Calibration Particles (BD Biosciences).
-
bioRxiv - Immunology 2024Quote: ... Surface staining was performed for 15 min in the presence of 1 mg/ml of Beriglobin (CSL Behring) with the following fluorochrome-conjugated antibodies titrated to their optimal concentrations: anti-CD3-FITC (Miltenyi Biotec), anti-CD4-VioGreen (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... anti-CD8-VioBlue (Miltenyi Biotec), anti-CD38-APC (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... anti-CD38-APC (Miltenyi Biotec), and anti-HLA-DR-PerCpVio700 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... and anti-HLA-DR-PerCpVio700 (Miltenyi Biotec). During the last 10 min of incubation ...
-
bioRxiv - Immunology 2024Quote: ... and TGFβ (Miltenyi Biotec), or 10 µg/ml LPS (InvivoGen).
-
bioRxiv - Immunology 2024Quote: ... anti-CD4-VioGreen (Miltenyi Biotec), anti-CD8-VioBlue (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... Intracellular staining was carried out for 30 min in the dark at room temperature with anti-4-1BB-PE (Miltenyi Biotec), anti-CD40L- PEVio770 (Miltenyi Biotec) ...
-
bioRxiv - Immunology 2024Quote: ... The remaining tissue was placed in a gentleMACS C-tube and processed using the gentleMACS Mouse Adipose Tissue Dissociation Kit and the gentleMACS Octo Dissociator with heaters (Miltenyi Biotec). Cell suspensions were diluted to 107 cells/mL and kept on ice ...
-
bioRxiv - Immunology 2024Quote: ... CD4 4+ T cells and total T cells were purified from cell suspensions using Mouse CD4+ T cell and total T cell Isolation Kits from Miltenyi Biotec (Auburn, CA, USA) (Supplemental material) ...
-
bioRxiv - Immunology 2024Quote: ... Tumors were cut into small pieces using scissors before being dissociated using the m_impTumor01.01 protocol on a GentleMACs dissociator (Miltenyi Biotec). The tumor samples were then incubated with shaking for 30 min at 37°C ...
-
bioRxiv - Immunology 2024Quote: ... using human CD14 Microbeads and LS columns (Miltenyi Biotec). Cells were then plated at 1.6×106 cells in 6-well plates and allowed to differentiate for five to seven days in RPMI-1640 medium (GIBCO) ...
-
bioRxiv - Immunology 2024Quote: ... Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (Miltenyi Biotec). Monocytes (>95% CD14+ cells ...
-
bioRxiv - Immunology 2024Quote: ... washed with MACS buffer (Miltenyi biotec), and filtered with 40μm strainer ...
-
bioRxiv - Immunology 2024Quote: ... Samples were digested (100µg/ml DNase and 0.4 mg/ml collagenase in 10% FBS/RPMI) using GentleMacs Dissociator (Miltenyi biotec), followed by filtering with 70μm strainer using 10% FBS/RPMI ...
-
bioRxiv - Immunology 2024Quote: ... The resulting cell suspensions were then used for magnetic-activated cell sorting (MACS)-based purification using MS MACS columns (Miltenyi Biotec) following the manufacturer’s directions ...
-
bioRxiv - Immunology 2024Quote: ... Samples were then labeled with CD45 MicroBeads (Miltenyi Biotec) at 4°C for 15 minutes ...
-
bioRxiv - Immunology 2024Quote: ... for MACS separation using anti-CD45 magnetic microbeads (Miltenyi Biotec, 130-052-301) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... tumors and spleens underwent single-cell processing with a Mouse Tumor Dissociation Kit (#130-096-730; Miltenyi Biotec) and a Mouse Spleen Dissociation Kit (#130-095-929 ...
-
bioRxiv - Immunology 2024Quote: ... and splenic CD4+ T cells were enriched by the MACS kit (130-104-454, Miltenyi Biotec). The enriched CD4+ T cells were stained with antibody cocktails containing flow cytometric and Hashtag antibodies on ice for 30 minutes ...
-
bioRxiv - Immunology 2024Quote: ... MEFSK4-PE (clone mEF-SK4, 1:100, Miltenyi Biotec), CD64-PE/Cy7 (clone X54-5/7.1 ...
-
Kir6.1, a component of an ATP-sensitive potassium channel, regulates natural killer cell developmentbioRxiv - Immunology 2024Quote: ... NK-specific Kcnj8 KO and WT mice were injected intravenously with 100 µg poly I:C (Miltenyi Biotec) in 200 µL sterile PBS ...
-
bioRxiv - Immunology 2024Quote: ... using CD4 (Miltenyi Biotec, 130-117-043) or CD8a (Miltenyi Biotec ...
-
bioRxiv - Immunology 2024Quote: ... or CD8a (Miltenyi Biotec, 130-117-044) MicroBeads ...