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Citations for Miltenyi Biotec :
1301 - 1350 of 8036 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... collected with BD FACSDiva 6.3.1 software and analyzed using Flowlogic software (Miltenyi Biotec). All antibodies used for extracellular and intracellular stainings are listed in the supplemental table 2.
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bioRxiv - Immunology 2024Quote: Tumors were mechanically dissociated with scissors and digested enzymatically using the Tumor Dissociation Kit mouse and the gentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... After filtration through a 70 μm cell strainer (Miltenyi Biotec), the single-cell suspension was washed two times with complete RPMI-1640 ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+CD44- T lymphocytes were subsequently isolated using the CD8α T Cell Isolation Kit (Miltenyi biotec) through addition of an anti-CD44 biotinylated antibody (clone IM7 ...
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bioRxiv - Immunology 2024Quote: ... Brains were immediately removed and dissociated using a Neural Tissue Dissociation kit (P) (Miltenyi Biotec, #130-092-628). Cells were then resuspended in 30% Percoll solution diluted in RPMI medium ...
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bioRxiv - Immunology 2024Quote: ... F4/80+ macrophages were positively selected using Microbeads (Catalog# 130-110-443, Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and tumors were harvested and digested with Tumor Dissociation Kit (Miltenyi Biotec) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Dead cells were removed using the Dead Cell Removal Kit (Miltenyi Biotec). T cells were isolated from target K562 cells using the Dynabeads™ Untouched™ Human T Cells Kit (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... mouse and AutoMACS® Pro Separator (Miltenyi Biotec), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Antibody-labeled cells were depleted by labeling with anti-FITC MACS beads (Miltenyi Biotec, catalog# 130-048-701) and subsequent negative sorting using autoMACS Pro Separator (Miltenyi Biotec) ...
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bioRxiv - Immunology 2024Quote: ... and subsequent negative sorting using autoMACS Pro Separator (Miltenyi Biotec). The enriched CD4+ T cell fraction was further stained with PerCP-Cy5.5-labeled anti-CD4 mAb and APC-labeled anti-CD62L mAb ...
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bioRxiv - Immunology 2024Quote: ... and GentleMACS Dissociator (Miltenyi Biotec) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The lungs were processed with Lung Dissociation Kit (Miltenyi Biotec, catalog# 130-095-927) and GentleMACS Dissociator (Miltenyi Biotec ...
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bioRxiv - Immunology 2024Quote: ... we enriched CD8+ T cells using a mouse CD8α T Cell Isolation kit (Miltenyi Biotec) to >95% purity as verified by flow cytometry (data not shown) ...
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bioRxiv - Immunology 2024Quote: ... securely placed in a MACSmix tube rotator (Miltenyi Biotec) in an incubator at 37°C with 5%CO2 ...
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bioRxiv - Immunology 2024Quote: ... total CD3+CD4+T cells or memory CD3+CD4+T cells were isolated using the human CD4+T Cell Isolation Kit or Memory CD4+T Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany), for respective cell types ...
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bioRxiv - Immunology 2024Quote: ... and anti-CD28 antibody coated beads (Miltenyi Biotec, Bergisch Gladbach, Germany) at a ratio of 3:1 (beads:cells) ...
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bioRxiv - Immunology 2024Quote: ... 0.5% BSA in PBS) and then incubated with 20 μl of anti-APC and anti-PE microbeads (Miltenyi Biotec, Cat #: 130-090-855 and 130-048-801) in 100 μl of MACS buffer at 4°C for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... the cells were resuspended in 1 mL of MACS buffer and applied to LS columns (Miltenyi Biotec, Cat #: 130-042-401) for enriching tetramer-bound T cells ...
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bioRxiv - Immunology 2024Quote: Primary human monocytes were isolated from PBMCs using negative selection human classical monocyte isolation kit (Miltenyi Biotec, 130-117-337) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... tightly placed in the QuadroMACS™ Separator (Miltenyi Biotec).
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bioRxiv - Immunology 2024Quote: ... SARS-CoV-2 Prot_S 130127953) (0.6 nmol/mL each) (Miltenyi Biotec; Bergisch Gladbach, Germany). After 6 days of incubation at 37°C ...
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bioRxiv - Immunology 2024Quote: ... was realized using the SARS-CoV-2 RBD B-cell MicroBead kit (Miltenyi Biotec, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were mechanically and enzymatically isolated using the Nuclei Extraction Buffer (Miltenyi Biotec, 130-128-024) following manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated using mouse CD4 (L3T4) microbeads (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Each mouse sample was processed with one LC column (Miltenyi Biotec) tightly placed in the QuadroMACS™ Separator (Miltenyi Biotec).
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bioRxiv - Cancer Biology 2024Quote: ... Fresh tumor tissue was mechanically dissociated and enzymatically digested using the Brain Tumor Dissociation Kit (Miltenyi Biotec, 130-095-942) and gentleMACS dissociator (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2024Quote: ... and gentleMACS dissociator (Miltenyi Biotec, 130-096-427) for 30 min at 37 °C ...
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Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s diseasebioRxiv - Neuroscience 2024Quote: ... a debris removal solution (Cat #130-109-398, Miltenyi Biotec) was added to the nuclei suspension (and centrifuged at 3,000 g for 10 mins at 4°C ...
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FUS controls muscle differentiation and structure through LLPS mediated recruitment of MEF2 and ETV5bioRxiv - Neuroscience 2024Quote: ... and filtered through a 30 μm pre-separation filter (Miltenyi Biotec, 130-041-407). Then a total of 250,000 cells per well were seeded on PLO and laminin coated glass coverslips in 24-well plates ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 ng/mL FGF-2 (Miltenyi Biotec, #130-093-564), and 100 ng/mL VEGF-A were carefully added to the center of each well ...
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bioRxiv - Plant Biology 2024Quote: ... membranes were probed overnight at 4°C with the corresponding antibodies : anti-GFP-conjugated with HRP (Miltenyi Biotec 130-091-833, 1:5000), anti-RFP (Abcam 34767 ...
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bioRxiv - Microbiology 2024Quote: ... CD34+ HSPC were isolated from human fetal liver obtained from the UCLA CFAR Gene and Cellular Therapy Core Laboratory (Los Angeles, California) or Advanced BioScience Resources, Inc (Alameda, CA) using a CD34+ microbead isolation kit (Miltenyi Biotec, Auburn, CA).
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bioRxiv - Immunology 2024Quote: ... followed by labelling with magnetic anti-PE MicroBeads (Miltenyi Biotec, Bergisch Gladbach, Germany). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... and presentation of SIINFEKL peptide in the context of MHC Class I on the surface of MC-38 cells was determined by flow cytometry (H-2Kb/SIINFEKL, REA1002, Miltenyi Biotec). The MC-38 cell line was maintained as a pool ...
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bioRxiv - Immunology 2024Quote: ... anti-REA Comp beads (Miltenyi Biotec) and unstained cells and determined on the instrument using inbuilt software ...
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bioRxiv - Immunology 2024Quote: CD8 T cells were isolated from spleens of OT-1 mice using the CD8 mouse isolation kit (Miltenyi Biotec) according to the instructions of the manufacturer ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T cells were purified using MACS Miltenyi mouse CD8α T Cell Isolation kit (Miltenyi Biotec 130-104-075) before proceeding to single-cell emulsion with 15946 splenic CD8+ T cells and 11856 brain CD8+ T cells ...
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bioRxiv - Immunology 2024Quote: ... Tregs (CD4+ hCD2+ CD25+ DAPI-) were sorted from the spleen and LN (after B cell depletion with MACS CD19 microbeads, Miltenyi Biotec) of CD45.2 Foxp3-hCD2 WT and PI3KδD910A mice ...
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bioRxiv - Immunology 2024Quote: ... and MACS LS columns (Miltenyi Biotec) then sorted (CD11c+ DAPI-) ...
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bioRxiv - Immunology 2024Quote: ... OT-I T cells were purified with the MACS CD8a+ T cell isolation kit (Miltenyi Biotec) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... the organs were macerated using a tissue homogenizer (Miltenyi Biotec, Bergisch Gladbash, Germany) with PBS containing 1.5% Triton X-100 and 1% protease inhibitor cocktail (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... using the human NK cell negative selection kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The suspension was passed through a 100-µm filter (Miltenyi Biotec), washed ...
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bioRxiv - Immunology 2024Quote: ... Imaging was performed using a LaVision Ultramicroscope II (Miltenyi Biotec). Image stacks were acquired with a step size of 6 µm at x2 magnification using an optic zoom with a numerical aperture of 9 µm ...
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bioRxiv - Immunology 2024Quote: ... following the manufacturer’s recommendations (LS columns, Miltenyi Biotec, Germany).
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bioRxiv - Immunology 2024Quote: ... The CD11c+ DCs were isolated from the PP-derived cells using CD11c Micro Beads Ultra Pure (Miltenyi Biotec). The total RNA was isolated from the DCs using TRIzolTM reagent (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... mouse were purchased from Miltenyi Biotec (Bergisch Gladbach, Germany).
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bioRxiv - Immunology 2024Quote: ... the cells were harvested and CD11c+ population was enriched using CD11c Micro Beads UltraPure (Miltenyi Biotec, Bergisch Gladbach, Germany). The quality of BMDCs was analyzed by flow cytometry in every culture ...
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bioRxiv - Immunology 2024Quote: ... Fc-receptor blocking was performed following manufacturer’s instructions (Miltenyi Biotec). Cells were washed twice with PBS and centrifuged (800 g ...